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Effect of Silk in Silk/PLGA Hybrid Films on Attachment and Proliferation of Human Aortic Endothelial Cells (실크/PLGA 하이브리드 필름에서 실크가 인간 대동맥 내피세포의 부착과 증식에 미치는 효과)

  • Lee, Jihye;Lee, Sojin;Kim, Seulji;Kim, Kyounghee;Kim, Younglae;Song, Jeongeun;Lee, Dongwon;Khang, Gilson
    • Polymer(Korea)
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    • v.37 no.2
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    • pp.127-134
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    • 2013
  • The vascular endothelial cells are the inner layers of blood vessels. It regulates the function of blood vessels and proliferation of vascular smooth muscle cells. Poly(lactide-co-glycolic acid) (PLGA) is a biodegradable synthetic polymer with a well-controlled degradation rate and an acceptable mechanical strength. It can be easily fabricated into many shapes. Silk consists of 18 amino acids. It found important for attaching cells cultured in vitro, and maintaining cell functions. In this study, we fabricated silk/PLGA biomaterial hybrid films of 0, 10, 20, 40 and 80 wt% silk. We performed MTT, SEM, ELISA, and immunocytochemistry analyses. We confirmed the adhesion and the proliferation of HAECs on silk/PLGA according to the content of silk, and 40 wt% silk/PLGA hybrid films have superior adhesion and proliferation properties. These results demonstrate that silk/PLGA hybrid films provide suitable surfaces for HAECs, and there is the effect of silk on cell growth and proliferation.

Preparation and Characterization of $CaCO_3$ Encapsulation by PMMA Core-Shell latex (PMMA와 캡슐화된 $CaCO_3$ Core-Shell 라텍스 제조와 물성연구)

  • Lim, Jong-Min;Seul, Soo-Duk
    • Elastomers and Composites
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    • v.38 no.4
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    • pp.303-315
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    • 2003
  • Inorganic/organic composite particles were also synthesized by changing an initiator an it's concentration, concentration of an adsorbed surfactant, reaction temperature, and agitation speed in the presence of $CaCO_3$ adsorbed SDBS. The polymerization conditions were optimized according to the conversion of the core-shell composite particles. In the inorganic/organic core-shell composite particle polymerization, $CaCO_3$ absorbed surfactant SDBS of 0.5 wt % was prepared first and then core $CaCO_3$ was encapsulated by sequential emulsion polymerization using MMA, concentration of APS $3.16{\times}10^{-3}mol/L$ to minimize the formation of new PMMA particle during MMA shell polymerization. The structure characterization of the inorganic/organic core-shell particles was verified by measuring the decomposition degree of $CaCO_3$ using HCl solution. It was found that $CaCO_3$ was encapsulated by shell PMMA due to having excellent dispersion in the epoxy resin, smooth surface distinctly from spindle shape, and broad particle distribution after the capsulation.

Case Study on the Type of Subsidence using Seismic Refraction Survey (탄성파 굴절법을 사용한 지반침하 형태분석 적용사례)

  • Yun Sang-Ho;Ji Jun;Lee Doo Sung
    • 한국지구물리탐사학회:학술대회논문집
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    • 2000.09a
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    • pp.132-146
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    • 2000
  • Seismic refraction survey was performed for 10 lines along NE-SW and NW-SE directions above Nampoong gallery at Makyo-ri, Dogye, Samcheok, Kangwon-do. 48 geophones were laid in line with the interval of 1m, and a 5Kg hammer was used as a source at 5 points for each line. Data processing was done using reciprocal time method, GRM, and traveltime tomography which utilizes wavefront expansion method for forward process and STRT for inversion. The result shows that the first layer has its lower boundary between 3.49m and 8.88m. The P-wave velocity of the first and the second layer were estimated as 270${\~}$360m/s and 1550${\~}$1940m/s respectively. When the boundary of the first and second layer is smooth enough and the velocity difference is large enough, GRM has little advantage over reciprocal time method. The result of reciprocal method and traveltime tomography shows consistency. The northeast part of the boundary has syncline structure, which is similar to the topography above. This implies that the collapse of the cavities of Nampoong gallery result in the subsidence of the ground surface. The subsidence is in progress across the Youngdong railroad, therefore a proper reinforcement work is required.

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A Study on Characteristics of Magnetism from Hydrothermal Vent Area on Esmeralda Bank in Mariana Arc (Mariana 해령 Esmeralda Bank 해저열수분출 지역에서의 지자기 특성 연구)

  • Kim, Ho;Kim, Chang-Hwan;Jeong, Eui-Young;Park, Chan-Hong;Kim, Jong-Uk;Park, Chung-Hwa
    • 한국지구물리탐사학회:학술대회논문집
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    • 2008.10a
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    • pp.27-32
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    • 2008
  • Detailed bathymetry and magnetic survey data for Esmeralda Bank obtained by R/V Onnuri of Korea Ocean Research & Development Institute in September 2007 were analyzed to estimate the locations of possible hydrothermal vents. The shape of Esmeralda Bank is caldera type opened in the western part. The summit is very shallow, about 50 meter b.s.l. and the bottom is about 1300 meter b.s.l. The western part of Esmeralda Bank is more steeper and topographic irregular than eastern part, and showed the valley made by erosion or collapse. The magnetic anomaly patterns of Esmeralda Bank located low anomalies over the north and high anomalies over the south. The magnetic anomalies are steep over the summits and gently smooth over the deep bottom. Two low magnetization zones are located in the summit and westside of Esmeralda Bank. The low magnetization zones of the summits of Esmeralda Bank suggest the possible existence of hydrothermal vent.

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Development of a Direct Evaluation Method to Measure the Rancidity of Yeonhaeju Soybean (Bazaz) Powders during Storage via the Fluorescence Spectrum Test (Fluorescence spectrum test를 이용한 연해주 대두(Bazaz) 분말의 저장 중 산패도 측정법 개발)

  • Uhm, Joo-Tae;Yoon, Won-Byong
    • Food Science and Preservation
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    • v.19 no.5
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    • pp.639-644
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    • 2012
  • The rancidity of soybean (Glycine max L.) from Yeonhaeju, called "Bazaz", in powder forms was evaluated through a fluorescence spectrum test (FST). The results from the FST were validated by comparing the TBA and acid values. Soybean powders were stored in 25, and $90^{\circ}C$ for 20 days. The maximum excitation and maximum emission of fluorescent compounds generated from the soybean powder during storage were observed at the 360 nm and 430-440 nm wavelengths, respectively. The mean particle size of soybean powder was maintained at $40{\mu}m$ to avoid the dependence of the reaction area during measurement. According to the FST results, lipid oxidation did not actively progress during storage at $25^{\circ}C$. The fluorescence intensity (FI) from FST on the first day of storage was not significantly different from that on the last day of storage (day 20; p < 0.05), but the FI dramatically increased at $90^{\circ}C$. A smooth increase was observed in the initial stage; then, after 11 days of storage, the FI value increased by nearly 100% compared to that on the first day. The FI values were compared with TBA and acid values that were measured under the same storage conditions. All the values at $25^{\circ}C$ showed similar patterns during storage, but at $90^{\circ}C$, the FI and acid values showed similar patterns but the TBA decreased after reaching the maximum values on storage day 12. The results demonstrated that FST may be useful for measuring the rancidity of the powder form of soybean because it does not require extraction to measure the rancidity.

Comparative seed morphology of the tribe Alsineae (Caryophyllaceae) in Korea and its taxonomic implications (한국산 Alsineae (석죽과) 종자의 형태비교 및 분류학적 유용성)

  • Song, Jun-Ho;Kim, Ju-Mi;Ok, Min-Kyoung;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.369-379
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    • 2015
  • The seed morphology of 25 taxa (8 genera) of Alsineae (Alsinoideae, Caryophyllaceae) in Korea was examined using stereo (SM) and scanning electron microscopy (SEM) in order to evaluate their diagnostic values and taxonomic implications. Circular, comma-shaped, ellipsoid, ovate, pyriform, reniform, and sub-circular seed shapes were identified. The seeds of the examined taxa range from 0.28 to 4.10 mm in length and from 0.33 to 3.10 mm in width; the smallest one was found in Sagina japonica, while the largest was in Honkenya peploides var. major. A hilar appendage was found in Cerastium, Honkenya, Moehringia, and strophioles were only observed in the genus Moehringia. Based on the examination of epidermal cells of the seed surface, four micromorphological types can be distinguished, viz., the colliculate-papillate (Col-Pap), colliculate-columellate (Col-Cll), colliculate-simple (Col-Sim), and smooth (Sm) types. Descriptions of the testa shape, margin, and the degree of elevation of the anticlinal cell walls, and the outer periclinal cell walls are summarized for the tribe. Taxonomic implications of the seed micromorphological characters were also discussed, in comparison with an earlier taxonomic system.

Comparison of Anticariogenic Effect after Applying Fluoride Varnish on Sound and Artificial Caries Enamel (정상법랑질과 인공우식법랑질에 불소바니쉬 도포 후 항우식 효과 비교)

  • Jeong, Moon-Jin;Lim, Ji-Hyang;Min, Ji-Hye;Jeong, Soon-Jeong;Son, Jung-Hui;Lim, Do-Seon
    • Journal of dental hygiene science
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    • v.13 no.4
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    • pp.461-470
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    • 2013
  • In order to examine the anticariogenic effect after fluoride varnish was applied to sound enamel and artificial caries enamel, anterior teeth of healthy cattle were used and divided into four groups such as group 1 (sound enamel), group 2 (application of fluoride varnish to sound enamel), group 3 (artificial caries enamel) and group 4 (application of fluoride varnish to artificial caries enamel). Remineralization on the surface of enamel and changes of crystalline structure after demineralization were observed by using a field emission scanning electron microscope (FE-SEM). Quantitative analysis of Ca and P was measured by using the energy dispersive X-ray spectrophotometer (EDS). The following conclusions were obtained: 1)Surface pattern of enamel was the roughest in group 3 due to the defects caused by porosity and microcracks. Group 4, group 1 and group 2 were followed in order; 2) It was found that pattern of crystalline structures in a group of application of fluoride varnish and a group of no application showed bigger change in artificial caries enamel than that in sound enamel. In other words, groups 4 and 1 showed a similar pattern; 3) The contents of Ca and P were higher in groups of application of fluoride varnish (group 2 and group 4) than in groups of no application of fluoride varnish (group 1 and group 3). Taken results of this study together, in the case of application of fluoride varnish, crystalline structure was changed by remineralization even in the sound enamel. In particular, porous structures showed a smooth and uniform pattern due to the recalcification in the artificial caries enamel. In addition, according to results of EDS analysis, the contents of Ca and P were increased and it had great anticariogenic effects which inhibit decalcification of sound enamel and artificial caries enamel.

Design and Implementation of Response type of Flickering Green Signal System using Beacon Message (비콘메세지를 이용한 반응형 녹색점멸 신호시스템 설계 및 구현)

  • An, Hyo-In;Mun, Hyung-Jin;Kim, Chang-Geun
    • Journal of Digital Convergence
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    • v.14 no.11
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    • pp.241-247
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    • 2016
  • As a domestic traffic control signal system, either the system with which a traffic signal turns into green at regular intervals or the system with which an amber or a red signal flickers on local roads without heavy traffic at midnight has been utilized. However, when the former system is used for roads with light traffic at midnight, delays and congestion can be incurred. Besides, in case of the latter signal system, the risk of vehicle crash is high. This study proposes a response type of flickering green signal system that rearranges signal system after analyzing beacon messages including sensor data. The proposed system, on a trunk road or a branch road at midnight, makes the signal keep flickering in green; When a vehicle enters the range of RSE, the transfer coverage, it transmits beacon messages regularly and Agent System analyzes the messages and alters the signal. It is a system by which vehicles move following the altered signal system, which will not only ensure smooth flow but also prevent vehicles from crashing on a road with light traffic. As a result of a simulation, traffic throughput and the average waiting time displayed 10 to 30 percent better improvement than existing signal systems, in terms of performance.

Designed of rPP/d2w®/ZnO Nanocomposite Flexible Film for Food Packaging and Characterization on Mechanical and Antimicrobial Properties (산화분해촉매를 함유한 rPP/ZnO 나노컴포지트 유연식품포장필름 제조 및 물성 특성 연구)

  • Lee, Jin-kyoung;Gil, Bo-min;Lee, Dong-jin;Lee, Ik-mo
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.24 no.1
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    • pp.1-11
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    • 2018
  • In this study, pro-oxidant($d2w^{(R)}$) and rPP/ZnO nanocomposite flexible films for food packaging were prepared, and their mechanical and antimicrobial properties were investigated. As a result, the carbonyl index and hydroxyl index increased with exposured time to heat and UV rays. Surface analysis showed that the addition of zinc oxide improved the dispersibility and compatibility of the polymer, so that the surface of the composite film was smooth and the zinc oxide particles were smaller than the compared film. And it kept the physical properties by heat and UV ray blocking effect, and it worked to reduce decomposition. In the antimicrobial activity test, the microbial reduction rate was 3 logs or more at the use concentration of zinc oxide. The tensile strength was increased and the elongation was decreased. Oxidative degradability of multi-layered film in UV exposured for 72 hours, the molecular weight of the film decreased by 75.6%, 1,294 g/mol Mn and 5,920 g/mol Mw. In the safety analysis of food packaging materials, we obtained that are in standard of polypropylene, a food contact material of domestic law.

An Effective Crossover Filter Choice of Multi-Way Loudspeaker System (멀티웨이 스피커 시스템의 효과적인 크로스오버 필터 선택)

  • Kang, Seong-Hoon;Jung, Han-Kyo
    • The Journal of the Acoustical Society of Korea
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    • v.33 no.3
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    • pp.184-190
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    • 2014
  • Although it is ideal that the sound with the range of 20 ~ 20,000 Hz is produced by the loudspeaker system composing of only one loudspeaker unit, it is almost impossible now. Therefore, the audible frequency range is now produced by the loudspeaker system, which is consisted of more than two loudspeaker units. The multi-way loudspeaker system certainly requires a crossover filter, which is divided the audible frequency into low and high frequency ranges. The crossover frequency of filter usually is in the range of 1 ~ 4 kHz, the frequency range can largely affect to the sound articulation and quality. It is an example that the phase and amplitude at the crossover frequency can be different from each other, due to the variously reasons. Conversely, they can not produce the smooth frequency response due to the different distance between the two loudspeaker units and electrical properties. As a result, the sound articulation and quality can be degraded. Therefore, the phase and amplitude response at the crossover frequency has to be deeply considered, in order to exactly match the woofer and tweeter. In this study, it is proposed the methods which are the flat frequency and phase response to be obtained by adjusting of the delay time between loudspeaker units, and the choice of filter to be considered the roll-off properties of each unit. It is achieved the frequency response with ${\pm}1$ dB, and the sound articulation is also improved.