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Studies on Skin Permeation with Polymer Micelles and the Cell Penetrating Peptide of Pyrus Serotina Var Stem Extracts

  • An, Gyu Min;Park, Su In;Kim, Min Gi;Heo, Soo Hyeon;Shin, Moon Sam
    • Korean Chemical Engineering Research
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    • v.58 no.1
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    • pp.21-28
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    • 2020
  • The stem extract from Pyrus serotina var has natural antioxidant ability, but the extraction method does not result in a soluble compound in cosmetic formulations. This study investigated the cosmetic efficacy of the Pyrus serotina var stem extract and its epidermis permeation ability when combined with polymer micelles and a cell penetrating peptide. The total concentration of polyphenol compounds was determined to be 103.1644 ± 1.38 mg/g in the ethanol extract and 78.97 ± 1.45 mg/g in the hydrothermal extract. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effects were 55.94 ± 0.22% in the ethanol extract at 1,000 mg/L. Superoxide dismutase (SOD) activity rates were 104.05 ± 3.28% in the ethanol extract at 62.5 mg/L. The elastase inhibition rate was 67.21 ± 2.72% in the ethanol extract at 1,000 mg/L. An antimicrobial effect was observed in the Propionibacterium acnes strain. In the epidermal permeability experiment, it was confirmed that formulation of the polymer micelle containing the Pyrus serotina var stem extract and cell penetrating peptide (R6, hexa-D-arginine) showed small particle size and much better skin permeability. The cumulative amount of total Pyrus serotina var stem extract that penetrated to the skin over time increased over 24 hours in three formulations. The three formulations showed 51.61 ㎍/㎠ (Formulation 0), 75.97 ㎍/㎠ (Formulation 1) and 95.23 ㎍/㎠ (Formulation 2) skin penetration, respectively. Therefore, it was confirmed that the ethanol extracts of Pyrus serotina var stem showed good cosmetic efficacy and excellent epidermis permeation ability when combined with a polymer micelle and cell penetrating peptide. Thus, this extract has the potential to be used as a safe and natural cosmetic material in the future.

An optical fibre monitoring system for evaluating the performance of a soil nailed slope

  • Zhu, Hong-Hu;Ho, Albert N.L.;Yin, Jian-Hua;Sun, H.W.;Pei, Hua-Fu;Hong, Cheng-Yu
    • Smart Structures and Systems
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    • v.9 no.5
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    • pp.393-410
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    • 2012
  • Conventional geotechnical instrumentation techniques available for monitoring of slopes, especially soil-nailed slopes have limitations such as electromagnetic interference, low accuracy, poor longterm reliability and difficulty in mounting a series of strain sensors on a soil nail bar with a small-diameter. This paper presents a slope monitoring system based on fibre Bragg grating (FBG) sensing technology. This monitoring system is designed to perform long-term monitoring of slope movements, strains along soil nails, and other slope reinforcement elements. All these FBG sensors are fabricated and calibrated in laboratory and a trial of this monitoring system has been successfully conducted on a roadside slope in Hong Kong. As part of the slope stability improvement works, soil nails and a toe support soldier-pile wall were constructed. During the slope works, more than 100 FBG sensors were installed on a soil nail, a soldier pile, and an in- place inclinometer. The paper presents the layout and arrangement of the instruments as well as the installation procedures adopted. Monitoring data have been collected since March 2008. This trial has demonstrated the great potential of the optical fibre monitoring system for long-term monitoring of slope performance. The advantages of the slope monitoring system and experience gained in the field implementation are also discussed in the paper.

Effects of Load Carrying Capacity with Method of Application of Prestress on Long-Span Temporary Bridges (장지간 가설교량에서 프리스트레스의 도입방법과 텐던배치에 따른 내하력의 영향)

  • Sim, Jai-Hyun;Park, Jeong-Ung;Park, Kil-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.6
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    • pp.1275-1280
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    • 2009
  • In recent bridge design, studies on application of external prestress have actively been conducted. When prestress is applied to steel structures, the limit value of elastic strain with large load increases with reduction of steels, this method is economic in cost. According to study by Brodka (1969), steel plate bridges with prestress has an effect on cost saving of about 15% compared with structures without prestress. For that reason, our country recently adopted this method in construction of temporary bridges and various engineering technologies have been developed which made stress correction, droop correction and long-span construction possible with relatively small cross sections. This study verifies the method of application of prestress in temporary steel structures, the influence of high-strength tendon arrangement and the effects of composite structures of steel plates and high-strength tendons based on existing method.

Timing of Fusarium Head Blight Infection in Rice by Heading Stage

  • Kim, Yangseon;Kang, In Jeong;Shin, Dong Bum;Roh, Jae Hwan;Heu, Sunggi;Shim, Hyeong Kwon
    • Mycobiology
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    • v.46 no.3
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    • pp.283-286
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    • 2018
  • Fusarium graminearum causes the devastating plant disease Fusarium head blight and produces mycotoxins on small cultivated grains. To investigate the timeframe of F. graminearum infection during rice cultivation, a spore suspension of F. graminearum was applied to the rice cultivars Dongjin 1 and Nampyeongbyeo before and after the heading stage. The disease incidence rate was the highest (50%) directly after heading, when the greatest number of flowers were present, while only 10% of the rice infected 30 days after heading showed symptoms. To understand the mechanism of infection, an F. graminearum strain expressing green fluorescent protein (GFP) was inoculated, and the resulting infections were visually examined. Spores were found in all areas between the glume and inner seed, with the largest amount of GFP detected in the aleurone layer. When the inner part of the rice seed was infected, the pathogen was mainly observed in the embryo. These results suggest that F. graminearum migrates from the anthers to the ovaries and into the seeds during the flowering stage of rice. This study will contribute to uncovering the infection process of this pathogen in rice.

Rapid identification and toxin type analysis of Clostridium perfringens isolated from healthy or diseased stocks with necrotic enteritis in chichen (닭의 괴사성 장염 및 건강 계군에서 분리한 Clostridium perfringens의 신속동정 및 독소형 분석)

  • Kim, Hong-jib;Kang, Mun-il;Chung, Un-ik
    • Korean Journal of Veterinary Research
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    • v.37 no.1
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    • pp.137-146
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    • 1997
  • About Clostridium perfringens causing clinically necrotic enteritis or isolated from the intestinal contens of healthy chicken, We examined the usefulness of a rapid identification method by gas-liquid chromatography as well as the types of toxins. For this study, there were used 169 chickens including 116 broilers, 27 layers and 26 breeders which collected from 9 healty flock and 21 diseased flock showing necrotic enteritis. Among them, Cl perfringens was isolated from 30 chickens(17.8%) including 7 breeders(26.9%), 5 layers(18.5%) and 18 broilers(15.5%). Isolation of Cl perfringens was mainly from ceca (100%) and followed by small intestines(70.0%) and livers(16.7%), respectively. Average concentration of the pathogen in intestinal contents was $10^{3.8}CFU/g$ in cases occuring necrotic enteritis and on the contrary $10^{3.8}CFU/g$ in healthy cases. All isolates tested showed the same characterstics in biochemical tests compared to those in standard strain. Analysis of gas-liquid chromatography to volatile fatty acids produced by Cl perfringens in PYG broth showed the typical peaks of acetic and butyric acids compatible with the standard chromogram and was confirmed as a effective and reliable tool for rapid identification of the bacteria. Toxin types of 30 strains were mostly classified in A type(26 isolates) and the rest in C type(2 isolates) and unidentifed type(2 isolates). All the isolates were highly susceptible to amphicillin, amoxicillin and cephalothin.

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Analytic adherend deformation correction in the new ISO 11003-2 standard: Should it really be applied?

  • Ochsner, A.;Gegner, J.;Gracio, J.
    • Journal of Adhesion and Interface
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    • v.5 no.2
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    • pp.14-26
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    • 2004
  • For reliable determination of mechanical characteristics of adhesively bonded joints used e.g. as input data for computer-aided design of complex components, the thick-adherend tensile-shear test according to ISO 11003-2 is the most important material testing method. Although the total displacement of the joint is measured across the polymer layer directly in the overlap zone in order to minimize the influence of the stepped adherends, the substrate deformation must be taken into account within the framework of the evaluation of the shear modulus and the maximum shear strain, at least when high-strength adhesives are applied. In the standard ISO 11003-2 version of 1993, it was prescribed to perform the substrate deformation correction by means of testing a one-piece reference specimen. The authors, however, pointed to the excessive demands on the measuring accuracy of the extensometers connected with this technique in industrial practice and alternatively proposed a numerical deformation analysis of a dummy specimen. This idea of a mathematical correction was included in the revised ISO 11003-2 version of 2001 but in the simplified form of an analytical method based on Hooke's law of elasticity for small strains. In the present work, it is shown that both calculation techniques yield considerably discordant results. As experimental assessment would require high-precision distance determination (e.g. laser extensometer), finite element analyses of the deformation behavior of the bonded joint are performed in order to estimate the accuracy of the obtained substrate deformation corrections. These simulations reveal that the numerical correction technique based on the finite element deformation modeling of the reference specimen leads to considerably more realistic results.

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Dynamic Bearing Pressure of Inverted T-type Retaining Walls Subjected to Seismic Motion (지진시 동토압을 받는 역 T형 옹벽의 접지압 산정에 관한 연구)

  • Lee, Jin-Sun
    • Journal of the Earthquake Engineering Society of Korea
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    • v.16 no.2
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    • pp.35-45
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    • 2012
  • Pseudo static analysis using the Mononobe-Okabe method and numerical analysis considering a small strain nonlinearity from the soil were performed to determine the bearing pressure changes of the inverted T-type retaining wall subjected to earthquake motions. In many cases, an inverted 'T' type retaining wall of more than 10 m shows bearing capacity failure under earthquake conditions, despite showing sufficient bearing capacity during normal conditions. The most important reason for this is the change of the effective base area during an earthquake. In this paper, the change of the effective base area of an inverted 'T' type wall is analyzed by using finite difference element code (FLAC). In addition, the effect of dynamic bearing capacity coefficients (which has been suggested by several researchers but not adopted in current design codes and procedures) was verified.

Flexural Strength Evaluation of PSC Beam with Loss of PS Tendon Area (PS강재의 단면적 감소에 따른 PSC보의 휨강도 평가)

  • Park, Soon-Hyung;Kim, Yong-Tae;Youn, Seok-Goo;Kim, Eun-Kyum
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.309-312
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    • 2008
  • This paper describes ultimate load tests which were performed to show the effects of prestress loss and tendon corrosion on the flexural strength of post-tensioned concrete beams and the occurrence of wire fracture. Five test specimens were fabricated in laboratory with the variations of the prestress of tendons and the loss of tendon area. For two specimens, small area of tendon at the center of the beam was exposed by using diameter 25mm drill and the exposed tendon was corroded using accelerated corrosion equipment. During the tests, deflections, crack width, and strain changes were measured and acoustic events were monitored with two acoustic sensors. Tests results show that the ultimate flexural strength of test specimens with corroded tendons is smaller than the predicted flexural strength which is calculated considering the loss of tendon area. It is considered that estimation of flexural strength of PSC beams with corroded tendons is very complicated just based on the loss of tendon area obtained by one-side visual inspection.

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Finite element modelling of the shear behaviour of profiled composite walls incorporating steel-concrete interaction

  • Anwar Hossain, K.M.;Wright, H.D.
    • Structural Engineering and Mechanics
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    • v.21 no.6
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    • pp.659-676
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    • 2005
  • The novel form of composite walling system consists of two skins of profiled steel sheeting with an in-fill of concrete. The behaviour of such walling under in-plane shear is important in order to utilise this system as shear elements in a steel framed building. Steel sheet-concrete interface governs composite action, overall behaviour and failure modes of such walls. This paper describes the finite element (FE) modelling of the shear behaviour of walls with particular emphasis on the simulation of steel-concrete interface. The modelling of complex non-linear steel-concrete interaction in composite walls is conducted by using different FE models. Four FE models are developed and characterized by their approaches to simulate steel-concrete interface behaviour allowing either full or partial composite action. Non-linear interface or joint elements are introduced between steel and concrete to simulate partial composite action that allows steel-concrete in-plane slip or out of plane separation. The properties of such interface/joint elements are optimised through extensive parametric FE analysis using experimental results to achieve reliable and accurate simulation of actual steel-concrete interaction in a wall. The performance of developed FE models is validated through small-scale model tests. FE models are found to simulate strength, stiffness and strain characteristics reasonably well. The performance of a model with joint elements connecting steel and concrete layers is found better than full composite (without interface or joint elements) and other models with interface elements. The proposed FE model can be used to simulate the shear behaviour of composite walls in practical situation.

Formation of Induced Anisotropy in Amorphous Sm-Fe Based Alloy Thin Films (비정질 Sm-Fe계 합금 박막의 유도자기이방성 형성)

  • 송상훈;이덕열;한석희;김희중;임상호
    • Journal of the Korean Magnetics Society
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    • v.8 no.5
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    • pp.261-269
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    • 1998
  • Induced anisotropy with the energy of $6{\times}10^4\; J/m^3$ is obtained in amorphous Sm-Fe based thin films which are fabricated by rf magnetron sputtering under a magnetic field of 500~600 Oe. Compared with conventional thin films, the anisotropic thin films exhibit a similar "saturation" magnetostriction, but show a very large anisotropy in magnetorstiction which is of significant practical importance due to increased strain at a particular direction. It is shown from a systematic investigation over a wide composition range for binary Sm-Fe alloys that anisotropy is also induced, though small, during a normal sputtering procedure due to the stray field, and the largest anisotropy is observed in the composition range of 25~30 at.% Sm. Furthermore, induced anisotropy is also found to be formed by magnetic annealing, but the anisotropy energy is much smaller than that by magnetic sputtering. This may be because the volume diffusion by which atoms move during magnetic annealing to from induced anisotropy is much slower than the surface diffusion which is expected to be a dominant factor during magnetic sputtering.puttering.

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