• The Korean Journal of Physiology and Pharmacology
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• v.24 no.1
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• pp.89-99
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• 2020

Although microscopic analysis of tissue slides has been the basis for disease diagnosis for decades, intra- and inter-observer variabilities remain issues to be resolved. The recent introduction of digital scanners has allowed for using deep learning in the analysis of tissue images because many whole slide images (WSIs) are accessible to researchers. In the present study, we investigated the possibility of a deep learning-based, fully automated, computer-aided diagnosis system with WSIs from a stomach adenocarcinoma dataset. Three different convolutional neural network architectures were tested to determine the better architecture for tissue classifier. Each network was trained to classify small tissue patches into normal or tumor. Based on the patch-level classification, tumor probability heatmaps can be overlaid on tissue images. We observed three different tissue patterns, including clear normal, clear tumor and ambiguous cases. We suggest that longer inspection time can be assigned to ambiguous cases compared to clear normal cases, increasing the accuracy and efficiency of histopathologic diagnosis by pre-evaluating the status of the WSIs. When the classifier was tested with completely different WSI dataset, the performance was not optimal because of the different tissue preparation quality. By including a small amount of data from the new dataset for training, the performance for the new dataset was much enhanced. These results indicated that WSI dataset should include tissues prepared from many different preparation conditions to construct a generalized tissue classifier. Thus, multi-national/multi-center dataset should be built for the application of deep learning in the real world medical practice.

• The Journal of the Korea institute of electronic communication sciences
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• v.8 no.12
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• pp.1875-1882
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• 2013

Micro-array technology contributes numerous achievements such as ordering of gene network and integration of genomic. This technology is well established as means for investigating patterns of gene expression. DNA micro-arrays utilize Affymetric chips where a large quantity of DNA sequences may be synthesized. There are two general type of conventional DNA array spotter: contact and piezoelectric. The contact technology used spotting pin technology to make contact with the glass slide surface. This may caused damage or scratches to the surface matrix where protein will be contaminated and may not bind specifically. Piezoelectric technology available at this present time on the other hand requires the analyzer to print the result that can only be done within the laboratory despite of mass production. Therefore, in this paper, high-throughput technology is developed for providing greater consistency in feature spot without touching the glass slide surface.

• Journal of the Korean Society of Clothing and Textiles
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• v.34 no.4
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• pp.553-562
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• 2010

This survey investigates the conditions of summer working uniforms for contracted foodservice workers. The data were obtained from 67 workers through in-depth interviews (July 2005~October 2005). The results of study are as follows: The working environment changed to menu and cooking method (air temperature $28\sim37^{\circ}C$, humidity 72~86 %RH, radiant temperature $27\sim37^{\circ}C$, air velocity 0.14~0.37m/sec). They answered that the working environment has high temperatures, humidity, excessive noise, and liability to slide. The typical accidents were burns, cuts, slide, and ligament injuries in the workplace. Work duties consisted of cooking, serving food, washing, and cleaning up leftover food. All the employees carried out multi tasks. The primary working postures and motions were standing, crouching, and lifting. The female workers usually wore underwear (panty and brassiere), upper and lower work wear, aprons, waterproof-aprons, cotton-gloves, rubber-gloves, socks, and rubber-boots. The satisfaction of the uniform was relatively low for trousers and waterproof-aprons. The answer about the fit was generally "comfortable." They answered "back," "chest," and "head" were wet with perspiration during work. The uncomfortable parts were the crotch and neck. Questions concerning their satisfaction with the material of uniforms indicated a high rate of dissatisfaction, particularly for ventilation and absorbency. In case of the colors of the working uniform, workers preferred white color for the upper part, and black color for the lower part.

• The Korean Journal of Nuclear Medicine
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• v.4 no.1
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• pp.43-49
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• 1970

In recent years with development of immuno-electrophoresis, more acurate analysis of the serum protein became possible. However, there is few reports in the literature which investigated the changes of the immunoglobulin compared with electrophoretically fractioned serum thyroglobulin in the patients with various thyroid diseases. The purpose of this report is to investigate the changes of thyroglobulin in various thyroid diseases by the method of immuno-electrophoresis and to compare the results with.serum protein fractionated by the method of agar-gel micro-electrophoresis. Materials and Methods: Sera from 9 patients with diffuse toxic goiter, 2 nodular nontoxic goiter, 2 thyroiditis, 3 hypothy, roidism, 1 thyroid cancer, 7 cystic degeneration of the thyroid gland, and 10 normal subject were taken. All cases were confirmed by various laboratory thyroid function tests and thyroid needle biopsy. Immuno-electrophoretic analysis of the serum were performed by Scheidegger's modified micro-immuno-electrophoretic method. The antiserum was obtained from the Travenol Laboratories International, Hyland Products Division and was rabbit anti-human thyroglobulin. Microscope slide agar-gel electrophoresis for serum protein fractionation was performed at $4^{\circ}C$ using veronal buffer, pH 8.6 and ionic strength 0.05, with 54 volts and 2.8 mA for 60 minutes. The fractionated slide was stained with 0.1% thiazine red. The results were as follows: 1) Increase of immune-globulin macroglobulin (IgM), alphaglobulin, and immune-globulin A (IgA) by 95.8%, 100%, 29.2% respectively was found in the serum from various thyroid diseases. 2) Thyroglobulin fraction was found to be increased in 50%, no change in 41.7%, and no line in 8.3% with all of the various goiter patients. On the other hand, 10 normal control group showed only 2 cases of increase, 5 cases of no change and 3 cases of no line.

• Journal of fish pathology
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• v.33 no.1
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• pp.15-21
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• 2020

We found skin flukes in snubnose pompano (Trachinotus blochii) from a public aquarium and attempted clear identification of them to the species level by morphology and molecular analyses. Skin flukes were collected from snubnose pompano showing dyspnea, anorexia and mild hemorrhage on the skin. All the fish samples (n=2) were infected with the flukes on the skin, gill and eyes, covered with excessive mucus. The isolated worms were transferred for making slide specimen and PCR amplification targeting 18S rDNA, 28S rDNA, mitochondrial cytochrome c oxidase subunit 1 (mt cox1) and cytochrome b (Cytb) genes for further analyses. Morphology and measurements data of our slide specimen coincided with those of Neobenedenia girellae. The sequence data of 2 genes (28S rDNA and Cytb) and the phylogenetic trees revealed that our specimen consistently belonged to the N. girellae clade. For 18S rDNA and mt cox1 genes, there was no sequence of either of these 2 Neobenedenia species from the type host available in GenBank. This is the first record of N. girellae in snubnose pompano, but it is still unclear if the snubnose pompano is a natural host for N. girellae or not because N. girellae is known to have an unusual broad host range and the host-switching can occur particularly in captive conditions such as aquarium or aquaculture facilities.

• The KIPS Transactions:PartC
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• v.13C no.2 s.105
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• pp.249-258
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• 2006

WebDAV is an IETF standard protocol, which is an extended version of HTTP 1.1, supporting asynchronous collaborative authoring of various contents on the web. Recently, to precisely control access to the resources on a WebDAY server, the WebDAV Access Control Protocol was published. This protocol helps developers to implement advanced server functionalities in a convenient way by providing various control methods to the resources on the WebDAV server. Based on the WebDAV access control protocol, we developed Open Workspaces, which systematically support exchanging and sharing data for general visitors. An Open Workspace can be supplied as a Download-Only Open Workspace, an Upload-Only Open Workspace, or an Upload-Download Open Workspace. Any registered user can create these workspaces without bothering the system administrator. To support the Open Workspaces, we extended the Slide WebDAV server and the DAVExplorer WebDAV client, which are being used popularly.

• Korean Journal of Veterinary Research
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• v.43 no.1
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• pp.67-75
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• 2003

A total of 260 dogs were randomly selected from two different treed kennels that brucellosis has occurred (group 1, 126 dogs), and random selected breed kennel (group 2, 134 dogs), and monitored for Brucella canis (B. canis) by 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and bacterial culture method. For the differentiation, PCR-RFLP using omp-31, wbkA and per genes used for 52 of B canis strains (strain I) isolated in this study and 3 of B. canis strains (strain II) isolated in 1994 in Korea. 2ME-RSAT revealed that 63/126 dogs (50.0%) and 12/134 dogs (9.0%) were positive in group I and group II, respectively. Bacterial culture revealed that 47/126 dogs (37.3%) and 5/134 dogs (3.7%) were positive in group I and group II, respectively. As the results of PCR-RFLP, $\underline{omp}-31$ was amplified from all Brucella spp, except B. abortus. All B. canis isolates showed unique PCR-RFLP pattern following digestion with Bmel8I. However, all Brucella spp. showed the same PCR-RFLP pattern following digestion with SalI. PCR-RFLP analysis of wbkA revealed that all Brucella spp. showed the same pattern following digestion with HindIII. PCR-RFLP analysis of per revealed that B. abortus 544 and B. melitensis 63/9 showed the same pattern, but different from B. suis and B. canis following digestion with HindIII.

• Journal of the Ergonomics Society of Korea
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• v.33 no.2
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• pp.155-165
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• 2014

Objective: The aim of this study is to develop an office chair enabling to keep working at reclined sitting posture. Background: Sedentary workers are supposed to change the posture frequently during long hours of sitting. A reclined sitting position has been recommended to reduce disc pressure. But slumped sitting posture caused by the buttock sliding forward without any adjustment of back reclining is commonly observed. The worker seems to have tendency to change the sitting posture maintaining working condition. We assumed the reason to be their hands movement away from the working space when tilting backward. Method: Slide mechanism allowing seat to move forward was designed to maintain the hand position in working space during reclining. A prototype was manufactured and tilting motion was analyzed using motion capture system. Four experiment chairs were tested including the manufactured prototype chair and three other commercial chairs. Results: A backward movements of the hand position were 13.0mm, 101.7mm, 156.1mm and 139.3mm at the prototype chair, compared to chair B, chair C and chair D, respectively. And the movement was remarkably small at the prototype chair. Conclusion: The developed seat sliding chair allows back tilting maintaining hand position at working space. We expect the user tilting back more often than normal tilting chair during seated work. But further investigation is required to figure out the effectiveness of the developed chair using prolonged working hours. Application: The developed office chair directly affects commercialization.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.40 no.4
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• pp.232-239
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• 2003

This paper discusses the design and implementation of a human-oriented interface based on computer vision that efficiently controls presentation slides. The user does not have to be confined to a keyboard or mouse any more, and can move around more freely because slides for presentation can be up and down using a general laser pointer that is used for presentation. Regions for virtual buttons are set on the slide so that the user can conveniently point the buttons using the laser pointer. We have proposed a simple and efficient method that computes the button areas in the image without complicated calibration. The proposed method has been implemented based on Microsoft PowerPoint ; moreover it can be applied to other PowerPoint-like presentation softwares. Our method for human-centered slide control enables the user to give audiences a more interactive presentation in a natural way.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.22 no.2
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• pp.206-215
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• 2013

In this paper, a synchronization control technique of dual-servo motor driven press system is proposed. An independent cascade PID control technique has been applied to the conventional press system for advancement of control stability. However, it is not easy to reduce synchronous error using the independent cascade PID control technique when some different load disturbances are involved in each motor. The eccentric error of the slide caused by the problem degrade the control performance of the BDC(Bottom Dead Center). In order to achieve reduction of the synchronous error between two servo motors and accurate position control simultaneously, a new control scheme comprised with cascade PID control loop and cross-coupling loop is proposed. In simulation using Matlab SIMULINK, the AC servo system is designed. The control performance of proposed technique is compared with conventional control technique to the model of AC servo system. Also, the sub-scale model of dual-servo motor driven press system which can replicate the slide motion is constructed for experimental verification for the performance of the proposed control technique. The cross-coupling control technique reveals more precise and stable performances in the position and synchronization controls.