• BMB Reports
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• v.31 no.1
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• pp.25-30
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• 1998

Kinetic studies of L-Alanine dehydrogenase from Bacillus subtilis-catalyzed reactions in the presence of $Zn^{2+}$ were carried out. The substrate (L-alanine) saturation curve is hyperbolic in the absence of the metal ion but it becomes sigmoidal when $Zn^{2+}$ is added to the reaction mixture indicating the positive cooperative binding of the substrate in the presence of zinc ion. The cooperativity of substrate binding depends on the xinc ion concentration: the Hill coefficients ($n_H$) varied from 1.0 to 1.95 when the zinc ion concentration varied from 0 to $60\;{\mu}m$. The inhibition of AlaDH by $Zn^{2+}$ is reversible and noncompetitive with respect to $NAD^+$ ($K_i\;=\;5.28{\times}10^{-5}\;M$). $Zn^{2+}$ itself binds to AlaDH with positive cooperativity and the cooperativity is independent of substrate concentration. The Hill coefficients of substrate biding in the presence of $Zn^{2+}$ are not affected by the enzyme concentration indicating that $Zn^{2+}$ binding does not change the polymerization-depolymerization equilibria of the enzyme. Among other metal ions, $Zn^{2+}$ appears to be a specific reversible inhibitor inducing conformational change through the intersubunit interaction. These results indicate that $Zn^{2+}$ is an allosteric competitive inhibitor and substrate being a non-cooperative per se, excludes the $Zn^{2+}$ from its binding site and thus exhibits positive cooperativity. The allosteric mechanism of AlaDh from Bacillus subtilis is consistent with both MWC and Koshland's allosteric model.

• Korean Journal of Animal Reproduction
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• v.18 no.3
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• pp.191-197
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• 1994

It is generally known that mutations in any of several genes encoding photoreceptor-specific proteins have resulted in retinitis pigmentosa (RP), a disease characterized by losing photoreceptor function with progressive degeneration of photoreceptor cells and eventually leading to blindness. To study the procure and cure of photoreceptor degeneration, we produced transgenic mice. Transgene consisted of a 12.5kb genomic DNA fragment that contains mutated pig rhodopsin gene (Pro-347-Ser) including both the 5'-franking (4.0 kb) and the 3'-franking (2.9 kb) sequences. This gene was used for the production of transgenic mouse. The mutated rhodopsin DNA was microinjected into male pronuclei of fertilized mouse (C57BL /6]) embryos. We detected transgenic animals harboring mutated rhodopsin gene by PCR and Southern blot analysis. These transgenic mice showed stable transmission of microinjected rhodopsin gene into their offspring. Therefore these animals will provide a novel approach to study the mechanism of the photoreceptor degeneration and be provided as a disease model for the treatment of the blind in human.

• Journal of Pharmaceutical Investigation
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• v.24 no.3
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• pp.155-165
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• 1994

Some mucoadhesive polymers such as hydroxypropylcelluose (HPC) and carbopol-934 (CP) have been employed for the preparation of mucoadhesive polymeric systems, and their physical properties including mucoadhesion, swelling, and drug release were evaluated. A new simple experimental technique that can quantitatively measure the bioadhesive properties of various polymeric systems has been developed by the methods of detachment force test. As the polymeric systems, the discs of freeze-dried HPC/CP solid dispersions were prepared. The mucosa used in these tests were upper, middle, and lower parts of small intestine of male rats weighing $300{\sim}350\;g$. Detachment forces were increased as the mole fraction of CP increased in discs of HPC/CP solid dispersions. In the points of intestinal site dependence of mucoadhesion, the solid dispersions revealed non-specific mucoadhesion to the intestine. Swelling and drug release characteristics of mucoadhesive polymeric systems were studied extensively to find out the feasibility for the oral controlled delivery systems. Swelling ratio, expressed as the final height/initial height, has been determined in various pH buffer solutions. Hydrochlorothiazide (HCT) was employed as a model drug for release study. Apparent swelling and drug release rate constants, $K_s$ and $K_r$ respectively, were obtained from the square-root time plot of either swelling ratio or released amount of drug, particularly for the time periods before reaching the equilibrium. As a result, the swelling ratio of HPC/CP solid dispersions was increased as the weight percentage of CP increased. Similarly, the release of HCT from the solid dispersions was dependent on pH changes and CP contents, resulted in the slower release of HCT with the increases of pH and CP contents.

• Applied Microscopy
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• v.28 no.1
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• pp.91-105
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• 1998

The present study is undertaken to investigate the hypertrophical changes of the corpus allatum (corpora allata, CA) after the ovariectomy in Blattella germanica. In particular, the ultrastructural aspects of the normal and ovariectomized conditions, and induced factors of the hypertrophic phenomenon are focused. Ultrastructure of the CA from an immediately emergent adult is similar to that of the last larval stage that has stopped secreting juvenile hormone. The CA is composed of undifferentiated cells, exhibiting an electron-lucent matrix, a few mitochondria and less smooth endoplasmic reticulum. The karyoplasm occupy most of the cytoplasm. Electron-dense materials are filled with the intercellular spaces and gap junctions are also found. Almost no ultrastructural changes have been noticed during seven days until the oviposition. However, considerable changes in structure have been detected soon after the oviposition. Mitochondria are increased dramatically in number and cristae, and changed to the filamentous form with a high electron density. In addition, Golgi complexes, microtubules, and polysomes are also increased. After an oviposition, the total volume of the CA are decreased again. The volume of the CA are increased continuously, hypertrophy, after the ovariectomy. Morphological aspects of the CA in an early stage after the removal are similar to the structure of the secondary egg maturation. Large and electron dense globules are observed in the ovariectomized CA cytoplasms and they are present in those cells for a long period of time. Yet such a hypertrophical phenomenon occur only in the specific cells. The hypertrophy are caused by hollowing the part of the CA cells and later filling such site with polysomes. In 42 days after the ovariectomy, the nuclear membranes disappear in the CA cells, thus, exhibiting the prokaryotic-like features. Some results of the current study will contribute to the establishment of the model that explain unusual changes accompanied by certain treatment in insects and/or further in animals.

• Earthquakes and Structures
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• v.9 no.3
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• pp.603-623
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• 2015

The scale and nature of the recent earthquakes in the world and the related earthquake disaster index coerce the concerned community to become anxious about it. Therefore, it is crucial that seismic lateral load effect will be appropriately considered in structural design. Application of seismic isolation system stands as a consistent alternative against this hazard. The objective of the study is to evaluate the structural and economic feasibility of reinforced concrete (RC) buildings with base isolation located in medium risk seismic region. Linear and nonlinear dynamic analyses as well as linear static analysis under site-specific bi-directional seismic excitation have been carried out for both fixed based (FB) and base isolated (BI) buildings in the present study. The superstructure and base of buildings are modeled in a 3D finite element model by consistent mass approach having six degrees of freedom at each node. The floor slabs are simulated as rigid diaphragms. Lead rubber bearing (LRB) and High damping rubber bearing (HDRB) are used as isolation device. Change of structural behaviors and savings in construction costing are evaluated. The study shows that for low to medium rise buildings, isolators can reduce muscular amount of base shears, base moments and floor accelerations for building at soft to medium stiff soil. Allowable higher horizontal displacement induces structural flexibility. Though incorporating isolator increases the outlay, overall structural cost may be reduced. The application of base isolation system confirms a potential to be used as a viable solution in economic building design.

• Journal of the Korea Society of Computer and Information
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• v.20 no.11
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• pp.143-150
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• 2015

Social network services can be defined as an individual web page which enables online, human-relationship building by collecting useful information and sharing it with specific or unspecific people. Recently, as the social network services(SNS) such as Twitter and Facebook have been paid attention in many fields of the society. SNSs are also one of the fastest channels to get news which people may not be able to see on TV or newspaper. The number of people who feel they are benefiting from social network services are increasing dramatically. A number of researches about SNS are underway. The study based on the Technology Acceptance Model empirically investigates the relationship between characteristics of SNS (system, service, information, and emotional) and user satisfaction of SNS. The study also analyzes how the relationshipa between SNS characteristics, satisfaction and user acceptance are moderated by country type of SNS users and inclination toward SNS acceptance. To achieve these research purposes, the study conducted various statistical analyses using questionnaire of the Korean and Chinese SNS users. The results of the study are followings. First, SNS characteristics have a positive effect to the user satisfaction. Second, SNS satisfaction have a positive effect to the user acceptance. Third, the relationship between SNS characteristics and user satisfaction is moderated by the country type of SNS users and inclination toward SNS acceptance. The study results could provide some implications to researchers who have interest in studying SNS, also could help business managers to operate and develop their SNS site more effectively.

• Journal of the Korea Institute of Building Construction
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• v.2 no.4
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• pp.123-129
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• 2002

This research is aiming to specify the requirement of the investment such as initial cost, running cost earning rate to make effective investment considering the purpose of remodeling and economical value of store building enough to meet the initial purpose of remodeling. The review of earning rate for economical evaluation was performed by the on-site auditing on the structure and function of the building and applying the assessment simulation program, which is to find the possible business model to identify the requirement of building owner through case study. After the research, the following results are obtained. First, it is important that many aspects should be carefully analysis and the best method should be selected as characteristics of remodeling can tie defer each other in their implementations. Second, though the remodeling of exist buildings to promote the functions has been applied. no suitable assessment tool has bee developed for deriding the level of remodeling in the view of economical efficiency so far. Third, the economical benefit was evaluated by analysing annual earning rate which is applied by investment items and recovery period for the investment. More specific data base should be established to apply the suggested economic accession in business enough to forecast the future circumstances. More researches should be promoted on this area continuously as well as the integrated economic evaluation of remodeling on existing building.

• Journal of the Korea Institute of Building Construction
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• v.17 no.3
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• pp.287-294
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• 2017

Recently the construction project is becoming large-sized, complicated, and modernize. This has increased the uncertainty of construction risk. Therefore, studies should be followed regarding scientifically identifying the risk factors, quantifying the frequency and severity of risk factors in order to develop a model that can quantitatively evaluate and manage the risk for response the increased risk in construction. To address the problem, this study analyze the probability distribution of risk causes, the probability of occurrence and frequency of the specific risk level through Monte Carlo simulation method based on the accident data caused at construction sites. In the end, this study derives quantitative analysis by analyzing the amount of risk and probability distributions of accident causes. The results of this study will be a basis for future quantitative risk management models and risk management research.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.21 no.4
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• pp.39-49
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• 2022

In this study, data on traffic volume and weight of small trucks were collected from three National Highways to identify the overload status of small trucks excluded from domestic overloaded vehicle enforcement. These data were classified according to the vehicle type, day of the week, and time of day to analyze the overload distributions by the vehicle total weight. From the analysis results by vehicle type, on average, the overload ratios for trucks of 1.5 tons or less, 2.5 tons or less, 3.5 tons or less were 6.2%, 31.7%, and 13.7%, respectively. In addition, for the same vehicle model, there was a difference by collection point, and the smaller the total weight of the small truck, the higher the overload ratio. From the results according to the day of the week, there was no tendency for the overload ratio to appear high on a specific day, but there was a predictable occurrence pattern at each site. In addition, from the results by the time of day, the overload ratio was the highest on average after lunch (13:00-15:59) at all sites.

• Korea Trade Review
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• v.43 no.3
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• pp.219-243
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• 2018

This study focused on the rapidly growing Chinese market by developing an evaluation model for overseas direct sales websites for Chinese consumers. In addition, we developed specific strategic alternatives for each sub-market by conducting multi-group analysis for each consumer's characteristics. Results showed that the level of concern about site use had a stronger impact on persistent use behavior. In addition, among antecedents that affect the level of assurance and concern, we found that these includes not only the perceived benefits and perceived risk factors identified in previous studies but also social-cultural factors. In addition, this study compares the evaluation of overseas direct sales website according to the level of involvement of users. This study analyzes the differences in coefficients among the paths and suggest practical implications for each group.