• BMB Reports
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• v.45 no.12
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• pp.748-753
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• 2012

A sequence of 10 amino acids at the C-terminus region of methylglyoxal synthase from Escherichia coli (EMGS) provides an arginine, which plays a crucial role in forming a salt bridge with a proximal aspartate residue in the neighboring subunit, consequently transferring the allosteric signal between subunits. In order to verify the role of arginine, the gene encoding MGS from a thermophile species, Thermus sp. GH5 (TMGS) lacking this arginine was cloned with an additional 30 bp sequence at the 3'-end and then expressed in form of a fusion TMGS with a 10 residual segment at the C-terminus ($TMGS^+$). The resulting recombinant enzyme showed a significant increase in cooperativity towards phosphate, reflected by a change in the Hill coefficient (nH) from 1.5 to 1.99. Experiments including site directed mutagenesis for Asp-10 in TMGS and $TMGS^+$, two dimentional structural survey, fluorescence and irreversible thermoinactivation were carried out to confirm this pathway.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.09a
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• pp.426-430
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• 2003

Subsurface biobarrier technology has potential applications to contain contaminated groundwater and/or to degrade toxic pollutants in groundwater. Effective biobarrier formation is need to assess of hydrogeologic characteristics and to conduct practical operation strategies and design based on this prior to design biobarrier. Thus, in this study, we examined hydrogeologic characteristics in biobarrier construction site. Hydraulic conductivities which calculated from slug test data have shown difference with each well as 1.20$\times$10$^{-3}$ -6.00$\times$10$^{-5}$ cm/sec. Tracer test is a method in which concentration of tracer solution during withdrawal in each well by vacuum extraction system is measured with time. Tracer solution was continuously injected by constant head tank. Measured tracer concentration versus time data were fitted to analytical solution of convection dispersion equation (CDE). The fitting data of CDE to the measured data at each extraction well yielded were 0.61cm/min(pore velocity), 5.38$\textrm{cm}^2$/min(dispersion coefficient) for discharge rate of 0.47 1/min and 1.75cm/min(pore velocity), 36.34$\textrm{cm}^2$/min(dispersion coefficient) for discharge rate of 0.93 1/min. As a result, we acquired fundamental parameters which need to design biobarrier and operation strategies.

• Journal of Korean Society for Atmospheric Environment
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• v.21 no.3
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• pp.285-293
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• 2005

This study was aimed at estimation of gas-particle partitioning of polychlorinated biphenyls (PCBs) in ambient air. The samples were collected at urban site in Japan from March 2002 to January 2003. The concentration of total PCBs (from 4 CB to 10 CB) and TEQ (Toxic equivalent) ranged from 62 to $247\;pg/m^3$ and from 2 to $14\;fgTEQ/m^3 $, respectively. The average contribution $(\%)$ of gas phase to total PCBs concentration was above $80\%$, which suggests that in the atmosphere PCBs predominantly existed in the gas phase. The weak correlations between total PCBs concentration and temperature was found. However this result was due to a typhoon during summer and raining during sampling period. The gas-particle partition coefficient (Kp) was obtained as a function of temperature. The partition ratio of gaseous and particulate phase PCBs can be estimated for an arbitrary temperature. The plot of gas/particle partition coefficient (log Kp) vs. sub-cooled liquid vapor pressure $(log\;P_L)$ had reasonable correlations for individual samples but the slope varied among the samples (coefficients of determination for log Kp versus log $P_L$ plot were> 0.76 $(p<0.0001)$, except for 3 samples). As a result, the variations in the slope among the sampling period may be due to change of temperature, raining during sampling period and wind in this study.

• Journal of Korean Society of Water and Wastewater
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• v.7 no.2
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• pp.9-23
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• 1993

Emission of hazardous and volatile organic chemicals from solid waste landfill site was become to important issue because of environmental pollution and health risk by such chemicals. Laboratory batch and continuous experiments were conducted respectively to elucidate isothermal sorption behaviors and transport phenomena(by gas through unsaturated solid waste layer) in wet solid waste-gas system. Source separated and size reduced refuse(bulky waste) and incinerated ash were used after controlling water content, and trichloroethylene(TCE) was chosen among many such chemicals because of it's generality among those man-created pollutants. Isothermal TCE sorption equilibria wet solid waste-gas system can be described in linear equation and partition coefficient in this system can be estimated approximately by the simple equation derived from schematic structure of the system. Transport equation modified by instantaneous equilibrium sorption fraction and kinetic sorption rate(overall mass transfer capacity coefficient) simulated well the column experiment results.

• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.204-208
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• 2004

In the forthcoming 21C, the barometer of cultural lives depends on that the water demand will increase or not. On the opposite site of that, the small watersheds will influence directly on how to cover the surface of watersheds with land use, no planning developing watersheds, and the rearrangement of small rivers. Espacially as the exordinary climatic phenomena, water resources and water content of the small watersheds will be confused oil exactly not to make a plan of water resources. This study area has four small watersheds groups in Gangwon-Do Province, that is, group I five small river watersheds including Changchoncheon etc., group II fiver rivers watersheds including to Hwalsanmogicheon etc., group III five small river watersheds including Singicheon etc., group IV including to Sabulanggolcheon etc. According to the land use such as dry field(or farm), ice field, forest land, building lot arid others, in small watersheds, the amount of runoff will be impacted by precipitation. The comparison between the runoff was getting from Kajiyama Formular and calculated runoff from multi-linear regressed equations by land use percentage was performed. Its correlation which was estimated by coefficient of correlation will be accepted or not, as approched 1.00000 values. As the monthly water resources amount is estimated by multi-linear regressed equations, we make a plan to demand and supply the water quantity from small river watersheds during any return periods.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.321-325
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• 2007

3개의 매개변수(location, scale, shape)로 이루어진 GEV와 GLO 분포는, 미국의 공식적인 홍수빈도 분포인 Log Pearson Type III와 함께 수문분야에서 중요한 위치를 차지하고 있다. 본 연구에서는 Monte Carlo 실험을 이용하여 GEV와 GLO 분포에서 서로 다른 두 지점의 유출량 자료를 생성하여 L-CV(L-moment Coefficient of Variation; $\tau_2$)와 L-CS(L-moment Coefficient of Skewness; $\tau_3$)를 추정하였으며, L-moment 추정값들 간의 교차상관$(\tau_2-\tau_2,\;\tau_3-\tau_3,\;\tau_2-\tau_3)$과 유출량 자료간의 교차상관의 관계를 Simple Power 함수를 이용하여 유도하였다. 실험 과정에서 GEV와 GLO 분포가 비현실적인 음수 유출량을 생성하여, 실험 결과에 큰 영향이 있음을 확인하여, 두 분포에서 생성된 유출량 자료에서 음수값을 제외한 GEV+와 GLO+ 분포를 이용하여 관계식을 유도하고 이를 GEV와 GLO 분포의 결과와도 비교하였다. 본 연구에서 도출된 관계식은 향후 Generalized Least Square 회귀식을 이용하여 홍수분포의 지역 매개변수를 추정하기 위해 활용성이 클 것으로 기대한다.

• Journal of Ocean Engineering and Technology
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• v.2 no.2
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• pp.51-60
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• 1988

A data transform is performed by using a point-electrode focusing method in order to obtain accurate and objective interpretation of the borehole normal resistivity data. Two new synthetic curves can be generated through the data transform. The one is an approximate apparent resistivity curve, which would be used to predict the true resistivity of the formation. The other one is a bed boundary coefficient curve, which would be used to distinguish bed boundaries. The accuracy of the normal data interpretation can be improved and this method takes much less computational time than a linear inversion technique. Moreover, this method does not require an initial guess model and limitation of number of unknown parameters. Since this algorithm can be run on a personal computer, an immediate interpretation would be possible at the field work site. If an additional set of electrodes(a=125cm)is attached to a normal resistivity tool which is being used (a=25cm, 50cm, 100cm), the apparent resistivity for the point-electrode focusing device can be calculated, and it would maximize the use of short and long normal resistivity data and promote the accuracy of the interpretation.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.33 no.8
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• pp.580-588
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• 2009

This paper summarizes the experimentally-measured performance of small-scale, vertical-axis wind turbine for the purpose of improving the aerodynamic efficiency and its controllability. The turbine is designed to have a Savonius-Type rotor with an inlet guide-vane and an side guide-vane so that it achieves a higher efficiency than any lift- or drag-based turbines. The main design factors for this high-efficient, vertical wind turbine are the number of blades (Z), and the aspect ratio of Height/Diameter (H/D) among many. The basic model has the diameter of 580mm, the height of 464mm, and the blade number of 10. The maximum power coefficient of 0.50 was experimentally measured for the above-mentioned specifications. The inlet-guide vane ensures the maximum efficiency when the angle of attack to the rotor blade lies between $15^{\circ}$ and $20^{\circ}$. This experimental results for the vertical-axis wind turbine can be applied to the preliminary design of turbine output curve based on the wind characteristics at the proposed site by controlling its aerodynamic performance given as a priori.

• Nuclear Engineering and Technology
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• v.53 no.4
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• pp.1318-1322
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• 2021

To unveil and delineate the elements applicable to the radiation protection of a femoral entry shield, calculate its mass attenuation coefficient, and demonstrate its dose reduction efficacy for interventional radiologist performing transarterial embolization (TAE) of ruptured hepatocellular carcinoma (rHCC). The lead equivalency of the shield was firstly validated. Electron microscopy was used to confirm the femoral entry shield being lead-free and to analyze the elemental content, with which the mass attenuation coefficient of the shield was calculated. An adult phantom, irradiated at the upper abdomen to simulate the TAE of rHCC, was used together with a dosimeter attached to the palm of a hand phantom. The dose rates at the hand phantom were measured, with the rHCC clinical protocol, without and with the femoral entry shield placed over the right femoral access site of the adult phantom. Without using the shield, the average hand dose rate was measured to be 0.325 µSv/sec. While using the shield, it was determined to be 0.110 µSv/sec. There was significant 66% dose reduction to the hand dose of IRs performing angiographic intervention with the femoral entry shield.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.19 no.1
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• pp.1-7
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• 2021

The pH dependence of sorption distribution coefficient (Kd) of Np(IV) on MX-80 in Ca-Na-Cl type solution with the ionic strength of 0.3 M, which was similar to one of the reference groundwaters in crystalline rock, was experimentally investigated under the reducing conditions. The overall trend of Kd on MX-80 was independent of pH at 5 ≤ pH ≤ 10 but increased as pH increased at pH ≤ 5. The 2-site protolysis non-electrostatic surface complexation and cation exchange model was applied to the experimentally measured pH dependence of Kd and the optimized surface complexation constants of Np(IV) sorption on MX-80 were estimated. The values of surface complexation constants in this work agreed relatively well with those in the Na-Ca-Cl solution previously evaluated, suggesting that compared to Na+, the competition of Ca2+ with Np(IV) for surface complexation on MX-80 was not much strong in Ca-Na-Cl solution. The sorption model well predicted the pH dependence of Kd values but slightly overestimated the sorption at the low pH region.