• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.39 no.5
• /
• pp.473-479
• /
• 1994

A seed coating system was developed to screen non-viable seeds in the Brassicaceae. The crops studied were radish, chinese cabbage, broccoli, cauliflower and brussel sprout. Sinapine leaked more from artificially deteriorated seeds than non-deteriorated seeds. Seed coating with cellulose was to trap the sinapine leakage in the non-viable Brassicaceae vegetable seeds. The seeds were first hydrated, then coated with cellulose powder to capture the leakage. Coated seeds were dried, then sorted two fractions-fluorescent seeds and non-fluorescent seeds under the UV light. The ratio of the fluorescent seeds were higher in bad seedlot than good one. The germination rate were increased 3∼35% by eliminating the fluorescent seeds in tested Brassica vegetable seeds. Sowing non-fluorescent seeds resulted in a greater percent normal seedling than non-coated seeds in all seedlots. The fluorescent seeds contained a high percentage of the dead and abnormal seedlings.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 1990.10a
• /
• pp.38-39
• /
• 1990

• Korean Journal of Pharmacognosy
• /
• v.18 no.1
• /
• pp.41-49
• /
• 1987

Mustard leaf and seed showed the protective effect on lipid peroxidation in mouse liver. Their active principle was turned out to be sinapine.

• Applied Biological Chemistry
• /
• v.40 no.3
• /
• pp.238-242
• /
• 1997

Seeds of five radish varieties were soaked in water for three hours and cellulose was coated. The seeds were classified as three groups in UV light; non-fluorescent(NF), partly fluorescent(PF), and fully fluorescent(FF) seeds. Germination rate was less in the order of NF>PF>FF seeds. The seed coat structure of NF seeds was dense and showed round shape, while those of PF and FF seeds were wrinkled or ruptured. The cotyledon and hypocotyl of NF seeds were normal, while those of PF and FF seeds were dwarf and showed some scars in the cotyledons.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.40 no.3
• /
• pp.314-321
• /
• 1995

Experiments were conducted to obtain information on separation of nonviable seeds from seed lots of rape, Chinese cabbage and radish, by nondestrutive ways. Seeds were artificially aged at 90% relative humidity(RH) and 45$^{\circ}C$ to get different seed qualities. Large amounts of amino acids, proteins and sinapine were leaked from the dead seeds into water in the course of 4~8 hours soaking, while high quality seeds did not leak in all crops. Percentage of normal seedlings from nonfluorescent cellulose coated seeds of rape, Chinese cabbage and radish under ultraviolet light was 96, 96 and 74%, while that of fluorescent seeds was 8, 9 and 1%, respectively. Cellulose coating and storage of Chinese cabbage and rape seeds at 60 and 75% RH for 3 months and 90% RH for 2 months did not deteriorate seed quality. But percentage of normal seedlings from nonfluorescent radish seeds stored at 60, 75 and 90% RHs for 3 months was 63, 64 and 2%, respectively.

• Korean Journal of Medicinal Crop Science
• /
• v.20 no.6
• /
• pp.415-420
• /
• 2012

In order to evaluate the effect of seed weight on different aspect of safflower (Carthamus tinctorius L.) seed germination and growth characteristics. Quantity of sinapine leaked from seed was greater as the viability of seeds was dropped by the time elapsed of seed aging model and long storage condition in safflower (Carthamus tinctorius L.). The cultivar of safflower was Jin-Sun and the seeds that are separated to three different weights of small, middle, and large were used in this experiment. Large seeds revealed the highest germination percent, coleoptiles fresh weight, coleoptiles dry weight, radicle fresh weight and 1000 seed weights than other seed weight. Seed weight had little effect on yield while seed number exerted a positive influence. Interestingly, yield per plant and its major components, number of capsules and capsule weights, revealed a negligible relationship with oil content.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.40 no.4
• /
• pp.533-541
• /
• 1995

The leakage characteristics of electrical conductivity, inorganic constituents-K, Ca, Mg and Na, total sugar and total amino acid from the exudates of some vegetable seeds of viable and non-viable(artificially aged) were quantified to get basic information about the detection of the non-viable seeds. The crops studied were radish, cabbage, broccoli, onion and carrot. The time course electrolyte leakage was different from viable and non-viable seed of cruciferae but not sensitive in onion and carrot seed In inorganic constituents, potassium leakage was the greatest amount and difference between viable and non-viable seeds, but Ca, Mg and Na leakages were not as much differences as potassium. Total sugar as glucose and total amino acid as glycin leaked a lot more in aged radish, cabbage and broccoli seed than non-aged seed and the large differences were appeared after 4 hour imbibition. As a results, in general the leakages from the aged seeds were greater than from the non-aged seeds in most components tested but they were varied depending on species or varieties and components.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.42 no.6
• /
• pp.783-789
• /
• 1997

This study was conducted to identify leakage of fluorescent substances out of 56 crops and to develop sorting methods of nonviable seeds of Chinese cabbage. Fluorescent substances were detected from 36 species, Chinese cabbage, radish, broccoli, cauliflower, egg plant and rape seed etc. In Chinese cabbage, the germination rate of non-sorted seeds decreased rapidly with ageing of seeds at 45$^{\circ}C$ and 100％. After Chinese cabbage seeds soaked for 4hrs and coated with cellulose, the sorting ratio of non fluorescent seeds were decreased 91.3％ to 1.7％ by increasing ageing periods from control to 8 days, while fluorescent seeds were increased drastically from 8.7％ to 98.3％. Also, incubation of water soaked dead seeds of Chinese cabbage treated with 0.5-1.0％ ninhydrin and 0.01N sodium thiosulfate for 30~60 minutes at 35$^{\circ}C$ developed purple color.

• Journal of the Korean Applied Science and Technology
• /
• v.33 no.1
• /
• pp.155-167
• /
• 2016

Surface-active substances in defatted rapeseed cake were obtained using a supercritical fluid extraction method. Then, it was purified by removing sinapine in the extract through a series of steps using a mixed solvent: diethyl ether and ethyl acetate (1:1, v/v). Emulsifying properties of purified surface-active substances were investigated, including fat globule size, zeta potentials and creaming stability and its antioxidant activity in emulsion systems were also studied by peroxide value and $^1H$-NMR spectrum. It was found that fat globules in emulsions with purified surface-active substances were much smaller than ones with the unpurified. In addition, as pH of the emulsion lowered and with increasing NaCl concentration in the emulsion, they were observed to increase, which led to worse creaming stability. These properties were reflected in changes of zeta potentials of emulsions. The oxidative stability was better in emulsions with purified surface-active substances than ones with Tween 20 or commercial lecithin, possibly resulted from the existence of sinapic acid in the extract. It was concluded that purified surface-active substances from defatted rapeseed cake could be simultaneously used as emulsifier and antioxidant agent in emulsion system.