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Classification of Safflower(Carthamus tinctorious L.) Collections by RAPD Analysis (RAPD 분석에 의한 홍화의 품종군 분류)

  • Bang, Kyong-Hwan;Kim, Young-Guk;Park, Hee-Woon;Seong, Nak-Sul;Cho, Joon-Hyeong;Kim, Hong-Sig;Cho, Yong-Gu
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.3
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    • pp.225-231
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    • 2001
  • This studies were conducted to provide the basic information on safflower collections and to identify the variations which could be utilized in safflower breeding programs, The RAPDs was used to clarify the genetic relationships among safflower collections and to classify them into distint genetic groups. Among 30 of 10 mer primers in RAPD analysis, twenty were selected as the appropriate primers for identification of the genetic characters in safflower collections. Amplified PCR showed the highly reproducible bands at $3.0{\sim}0.2kb$. The number of bands amplified with the each primer showed the variations ranged from 2 to 11, with the average of 5.6. Total of 111 bands were identified among 20 selected primers used in PCR reaction and 84 bands (75.7%) showed polymorphism. Based on the similarity value of 0.14 in dendrogram derived from the cluster analysis using RAPD-PCR, the 30 safflower collections were classified into 11 groups. The two main groups, VII and VIII included 7 collections (23%) and 8 collections (27%), respectively. Most of the collections in group VII were the Korean collections (85%).

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LED Sensitive Light System Development by Brain-wave (LED감성조명 장치 개발을 통한 뇌파분석)

  • Choi, Keum-Yeon;Eo, Ik-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.1
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    • pp.61-66
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    • 2010
  • The purpose of this experiment is to analyze the basic status of brain. Which are consist of rest, attention and concentration, of the brain by measuring the temperature of color by changing RGB color after manufacturing LED-illumination stand. Basic status (rest, attention and concentration) of experimenter were measured temperature of colors having three difference temperature like as $2,300^{\circ}K$, $4,000^{\circ}K$ and $6,000^{\circ}K$. The results was shown that experimenter feels more comfortable and relaxation by decreasing the temperature of color. For example we can see the little increase of concentration index at $4,000^{\circ}K$ condition and we can estimate that right brain can be more activated at the $4,000^{\circ}K$ condition. But we can not find out any different at the $6,000^{\circ}K$ condition. Main cause of no difference from the color temperature was the similarity of color temperature under the general fluorescent lamp. And interface temperature of radiant heat design results LED and PCB was approximately 80 degrees to COMSOL Multiphysics, and changed until approximately 50 degrees until a floor plane of PCB, and verification as arranged chip LED to metal PCB, and it was possible, and a near radiant heat design was confirmed to an approximate value of, as a result, acid manufacture.

WordNet-Based Category Utility Approach for Author Name Disambiguation (저자명 모호성 해결을 위한 개념망 기반 카테고리 유틸리티)

  • Kim, Je-Min;Park, Young-Tack
    • The KIPS Transactions:PartB
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    • v.16B no.3
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    • pp.225-232
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    • 2009
  • Author name disambiguation is essential for improving performance of document indexing, retrieval, and web search. Author name disambiguation resolves the conflict when multiple authors share the same name label. This paper introduces a novel approach which exploits ontologies and WordNet-based category utility for author name disambiguation. Our method utilizes author knowledge in the form of populated ontology that uses various types of properties: titles, abstracts and co-authors of papers and authors' affiliation. Author ontology has been constructed in the artificial intelligence and semantic web areas semi-automatically using OWL API and heuristics. Author name disambiguation determines the correct author from various candidate authors in the populated author ontology. Candidate authors are evaluated using proposed WordNet-based category utility to resolve disambiguation. Category utility is a tradeoff between intra-class similarity and inter-class dissimilarity of author instances, where author instances are described in terms of attribute-value pairs. WordNet-based category utility has been proposed to exploit concept information in WordNet for semantic analysis for disambiguation. Experiments using the WordNet-based category utility increase the number of disambiguation by about 10% compared with that of category utility, and increase the overall amount of accuracy by around 98%.

A Study on the Nonlinear Deterministic Characteristics of Stock Returns (주식 수익률의 비선형 결정론적 특성에 관한 연구)

  • Chang, Kyung-Chun;Kim, Hyun-Seok
    • The Korean Journal of Financial Management
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    • v.21 no.1
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    • pp.149-181
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    • 2004
  • In this study we perform empirical tests using KOSPI return to investigate the existence of nonlinear characteristics in the generating process of stock returns. There are three categories in empirical tests; the test of nonlinear dependence, nonlinear stochastic process and nonlinear deterministic chaos. According to the analysis of nonlinearity, stock returns are not normally distributed but leptokurtic, and appear to have nonlinear dependence. And it's decided that the nonlinear structure of stock returns can not be completely explained using nonlinear stochastic models of ARCH-type. Nonlinear deterministic chaos system is the feedback system, which the past incidents influence the present, and it is the fractal structure with self-similarity and has the sensitive dependence on initial conditions. To summarize the results of chaos analysis for KOSPI return, it is the persistent time series, which is not IID and has long memory, takes biased random walk, and is estimated to be fractal distribution. Also correlation dimension, as the approximation of fractal dimension, converged stably within 3 and 4, and maximum Lyapunov exponent has positive value. This suggests that chaotic attractor and the sensitive dependence on initial conditions exist in stock returns. These results fit into the characteristics of chaos system. Therefore it's decided that the generating process of stock returns has nonlinear deterministic structure and follow chaotic process.

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Effects of Forest Fire on the Forest Vegetation and Soil (I) - The First Year's Results after Fire at Mt. Gwanag - (황폐산지(荒廢山地)에서의 산불이 삼림식생(森林植生) 및 토양(土壤)에 미치는 영향(影響)에 관한 연구(研究)(I) - 관악산(冠岳山) 뱀골계곡(溪谷)에서의 초기영향(初期影響) -)

  • Woo, Bo Myeong;Kwon, Tae Ho
    • Journal of Korean Society of Forest Science
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    • v.62 no.1
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    • pp.43-52
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    • 1983
  • The purpose of this study was to measure the changes in soil properties and forest vegetation after surface fire, which had occured on June 5, 1983 in Mt. Gwanag, Kyunggi-do. Moisture content, organic matter and acidity of soil increased just after the fire and then dropped down up to those of unfired areas as time goes. Also total nitrogen, available phosphorous exchangeable base had the similar trend to moisture, organic matter and acidity. Most of the exchangeable bases in surface soil except for sodium were higher than those in sub-soil. No changes in soil texture by the fire were found. Increasers, decreasers, invaders and neutral species could be classified according to the relative importance value of each species. Species diversity was reduced just after the fire and increased gradually afterward. Diversity in the southeast slope was higher than that in the southwest slope. Due to the fire, evenness of woody plants decreased continuously while that of herbs increased. Species similarity was shown greater at fired areas than at unfired areas.

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Bacillus oryzicola sp. nov., an Endophytic Bacterium Isolated from the Roots of Rice with Antimicrobial, Plant Growth Promoting, and Systemic Resistance Inducing Activities in Rice

  • Chung, Eu Jin;Hossain, Mohammad Tofajjal;Khan, Ajmal;Kim, Kyung Hyun;Jeon, Che Ok;Chung, Young Ryun
    • The Plant Pathology Journal
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    • v.31 no.2
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    • pp.152-164
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    • 2015
  • Biological control of major rice diseases has been attempted in several rice-growing countries in Asia during the last few decades and its application using antagonistic bacteria has proved to be somewhat successful for controlling various fungal diseases in field trials. Two novel endophytic Bacillus species, designated strains YC7007 and $YC7010^T$, with antimicrobial, plant growth-promoting, and systemic resistance-inducing activities were isolated from the roots of rice in paddy fields at Jinju, Korea, and their multifunctional activities were analyzed. Strain YC7007 inhibited mycelial growth of major rice fungal pathogens strongly in vitro. Bacterial blight and panicle blight caused by Xanthomonas oryzae pv. oryzae (KACC 10208) and Burkholderia glumae (KACC 44022), respectively, were also suppressed effectively by drenching a bacterial suspension ($10^7cfu/ml$) of strain YC7007 on the rhizosphere of rice. Additionally, strain YC7007 promoted the growth of rice seedlings with higher germination rates and more tillers than the untreated control. The taxonomic position of the strains was also investigated. Phylogenetic analyses based on 16S rRNA gene sequences indicated that both strains belong to the genus Bacillus, with high similarity to the closely related strains, Bacillus siamensis KACC $15859^T$ (99.67%), Bacillus methylotrophicus KACC $13105^T$ (99.65%), Bacillus amyloliquefaciens subsp. plantarum KACC $17177^T$ (99.60%), and Bacillus tequilensis KACC $15944^T$ (99.45%). The DNA-DNA relatedness value between strain $YC7010^T$ and the most closely related strain, B. siamensis KACC $15859^T$ was $50.4{\pm}3.5%$, but it was $91.5{\pm}11.0%$ between two strains YC7007 and $YC7010^T$, indicating the same species. The major fatty acids of two strains were anteiso-$C_{15:0}$ and iso $C_{15:0}$. Both strains contained MK-7 as a major respiratory quinone system. The G+C contents of the genomic DNA of two strains were 50.5 mol% and 51.2 mol%, respectively. Based on these polyphasic studies, the two strains YC7007 and $YC7010^T$ represent novel species of the genus Bacillus, for which the name Bacillus oryzicola sp. nov. is proposed. The type strain is $YC7010^T$ (= KACC $18228^T$). Taken together, our findings suggest that novel endophytic Bacillus strains can be used for the biological control of rice diseases.

Differential Cytotoxic Effects of Jaspine B in Various Cancer Cells (다양한 암세포주에서 Jaspine B의 함암활성 비교)

  • Lee, Jihoon;Choi, Kwangik;Kwon, Mihwa;Lee, Dongjoo;Choi, Min-Koo;Song, Im-Sook
    • Journal of Life Science
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    • v.26 no.12
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    • pp.1392-1399
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    • 2016
  • Jaspine B is an anhydrophytosphingosine that is isolated from a marine sponge. Because of its structural similarity to sphingosine, it shows anti-cancer effects in human carcinomas. Therefore, this study aims to investigate its anti-proliferative effect on various cancer cells and to correlate its association with the intracellular accumulation of Jaspine B in relevant cancer cells. The anti-proliferative effect of Jaspine B in various cancer cells was determined by a cell viability test, and the intracellular concentration of Jaspine B in relevant cancer cells was determined using mass spectrometry coupled with liquid chromatography. The correlation coefficient and p value between the cytotoxicity and the cell accumulation of Jaspine B were determined using SPSS 16.1. The cytotoxicity of Jaspine B varied depending on the type of cancer cell when compared the $EC_{50}$ values of Jaspine B. Breast and melanoma cancer cells were susceptible to Jaspine B, whereas renal carcinoma cells were resistant. The intracellular concentrations of Jaspine B had a reciprocal correlation with the $EC_{50}$ values in the same cells (r = 0.838). The results suggested that the anti-proliferative effect of Jaspine B was associated with the cellular accumulation of this compound. However, Jaspine B was not a substrate for P-glycoprotein and breast cancer resistance protein, as major efflux pumps caused multidrug resistance. The maintenance of a high intracellular concentration is crucial for the cytotoxic effect of Jaspine B; however, efflux pumps may not be a controlling factor for Jaspine B-related resistance in cancer cells.

The Development of Image Processing System Using Area Camera for Feeding Lumber (영역카메라를 이용한 이송중인 제재목의 화상처리시스템 개발)

  • Kim, Byung Nam;Lee, Hyoung Woo;Kim, Kwang Mo
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.1
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    • pp.37-47
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    • 2009
  • For the inspection of wood, machine vision is the most common automated inspection method used at present. It is required to sort wood products by grade and to locate surface defects prior to cut-up. Many different sensing methods have been applied to inspection of wood including optical, ultrasonic, X-ray sensing in the wood industry. Nowadays the scanning system mainly employs CCD line-scan camera to meet the needs of accurate detection of lumber defects and real-time image processing. But this system needs exact feeding system and low deviation of lumber thickness. In this study low cost CCD area sensor was used for the development of image processing system for lumber being fed. When domestic red pine being fed on the conveyer belt, lumber images of irregular term of captured area were acquired because belt conveyor slipped between belt and roller. To overcome incorrect image merging by the unstable feeding speed of belt conveyor, it was applied template matching algorithm which was a measure of the similarity between the pattern of current image and the next one. Feeding the lumber over 13.8 m/min, general area sensor generates unreadable image pattern by the motion blur. The red channel of RGB filter showed a good performance for removing background of the green conveyor belt from merged image. Threshold value reduction method that was a image-based thresholding algorithm performed well for knot detection.

Intraspecific Genetic Relation of Wasabia japonica Matsum. Based on RAPD Analysis (RAPD를 이용한 고추냉이의 유연관계 분석)

  • Heo, Su-Jeong;Kwon, Soon-Bae;Byeon, Hak-Soo;Seo, Jeong-Sik;Yoo, Ki-Oug
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.1
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    • pp.31-35
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    • 2004
  • The genetic variation and intraspecific relationships between 10 individuals of seven cultivars and one Ulleungdo native of Wasabia japonica were investigated using RAPD (Randomly Amplified Polymorphic DNA) analysis. The 21 primers out of 50 random primers were amplified for all tested plants. The 68 (47.2%) among 144 bands derived from 21 primers showed polymorphism, and 3.2 bands per primer were observed. Number of bands per primer was ranged from 2 to 13, and average numbers were 6.8. The phenograms for 11 analyzed individuals by RAPD markers were not matched well with those of the result by morphological characters since they were clustered monophyletic at the similarity coefficient value ranged from 0.81 to 0.96. The Ulleungdo native individual was clustered sister to Daruma, Simanesairal, Sawa, and Hujidaruma cultivars. The RAPD markers were not useful to evaluate the intraspecific variations in Wasabia japonica cultivars, therefore need to more specific molecular phylogenetic characters such as AFLP technology and gene sequence of nuclear and chloroplast DNA.

The Chemotoxonoic Relationship of Vibrio cholerae non-O1 by Fatty Acid Compositions (지방산 조성에 의한 vibrio cholerae non-O1의 화학분류학적 관계)

  • 성희경;이원재;장동성
    • Journal of Food Hygiene and Safety
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    • v.13 no.2
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    • pp.149-154
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    • 1998
  • The authors attempted utilization of fatty acid composition of vibrios as a tool for identification of the strains. Fatty acid of 49 strains of Vibrio cholerae non-O1, V. cholerae O1, V mimicus, V vulinificus and V parahaemolyticus was analyzed by gas-liquid chromatography column. According to the statistical analysis of the fatty acid data, the relationship between the Vibrio species and serotypes of the strains was discussed. Forty one kinds of fatty acid were detected from the tested strains and 35 kinds of fatty acids among the detected fatty acids were significant factors to identify the vibrios. The predominant fatty acids were 16:0, 16:1 cis 9, 18:1 trans 9/6/cis 11 and 15:0 iso 2OH/16:1 cis 9 as above about 20% in total. Fatty acid compositions of the Vibrio species were an important factor in identifying their subspecies either predominant fatty acids or minor ones. According to the analysed results by a conventional statistical processing method (UPGMA) and prepared dendrogram, V cholerae non-01 had more closer relationship with V. mimicus compared with V. cholerae 01. Moreover, the distribution of hydroxy acid was a significant factor for identifying V cholerae subspecies. Comprising all the 10 serotypes detected from V. cholerae non-01 examined such as O2, O5, O8, O10, O14, O27, O37, O39, O45 and O69, we could group them into seven subspecies by cluster analysis with the similarity value of fatty acid composition as above 92%. It means that there is a significant relationship between serotypes and fatty acid composition of V. cholerae. These results indicated that numerical analysis of fatty acid composition data of V cholerae non-01 could classifY them into subspecies, and also which may provide a useful epidemiologic information or a basis for further analysis such as PCR and DNA probe analysis.

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