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Angiogenic Responce to Transmyocardial Mechanical Reveascularization(TMMR) with Polymer Myocardial Stent (고분자 중합체 심근 스템트를 이용한 기계적 경심근 혈류재건술의 혈관생성 반응)

  • Choi, Ho;Lee, Cheol-Joo;Moon, Kwang-Deok;Kim, Young-Jin;Kang, Joon-Kyu;Hong, Jun-Wha;Jee, Kyung-Soo;Han, Man-Jung;Cho, Sang-Ho
    • Journal of Chest Surgery
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    • v.33 no.6
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    • pp.494-501
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    • 2000
  • Background: Transmyocardial laser revascularization(TMLR) for revascularizing ischemic myocardium in patients was originally based on the assumption that laser channels remain their patency much longer. But recent studies show that laser channels did not remain open and that TMLR could achieve treatment benefits without long-term channel patency. The angiongencesis is currently thought to be induced by non-specific inflammatory response to mechanical tissue injury. This study is to evaluate hypothesis that various transmyocaridal mechanical revascularization(TMMR) may induce the angiogenic responses similar to that seen with TMLR, and transmyocaridal polymer stent revascularization(TMSR), the polymer stent in the myocardial tissue is hydrolyzed in 2 weeks, may enhance the non-specific inflammatory reaction resulting angiogenesis. Furthermore, polymer myocaridal stent channels remain long-term patency. Material and Method: Eight domestic pigs underwent ligation of the proximal circumflex artery, and 2 weeks later they were randomized to undergo transmycardial acupunctural revascularization (TMPR, Group I) of the left lateral wall with 18-G needle(n=2), to undergo transmyocardial (TMDR, Group II) with industrial 2mm steel drill(n=2), to undergo transmyocardial polymer stent revascularization (TMSR, Group III) after drilling the infarcted myocardium(n=2), the stent is poly(lactic acid-co-glycolic acid), which is self-degradated in the myocardium, and to a control group the ischemic zone was unterated(n=2). All the pigs were sacrificed after 4 weeks TMMR. Sections from the ischemic zone were submitted for vascular endothelial growth factor (VEGF) ELISA and histology. Result: There were makedly increase in the VEGF immunoassay in the ischemic zone of the TMMR group compared to the ischemic zone of the control group(control: each 30.85 and 43.15pg/mg protein, TMPR: each 44.14 and 68.61 pg/mg protein, TMDR: each 65.92 and 78.65 pg/mg protein, TMSR: each 177.39 and 168.87 pg/mg protein). TMSR channels caused greatest VEGF expression than channels made by other group and the polymer stent channels remained vacuole after 4 weeks. Conclusion: Transmyocardial polymer stent revascularization promoted the most angiogenci response by the VEGF immunoassay, although our study did not show the statistical significancy. The channels remained but the flow patency was not verified. Transmyocardial polymer stent revascularization (TMSR) is desirable in future experimental trials and in view of the significant cost implications comparable to that of laser.

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Morphological Characteristics and Pathogenicity of Streptococcus iniae (Streptococcus iniae의 형태학적 특성과 병원성)

  • Kim, Hyun-Jeong;Woo, Sung-Ho;Kim, Jin-Woo;Park, Soo-Il
    • Journal of fish pathology
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    • v.18 no.2
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    • pp.167-178
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    • 2005
  • Streptococcus iniae is one of the major bacterial pathogens of cultured olive flounder, Paralichthys olivaceus in Korea. This study was carried out to investigate the interaction between morphological characteristics and immune response such as phagocytosis and serum killing activity. They were differentiated phenotypically into two groups, the viscous colonies and the non-viscous colonies, but the strains in both groups were similar in physiological and biochemical characteristics. Electron microscopic examination of the viscous form revealed thick, electron-dense exopolysaccharide materials surrounding polycationic ferritin-stained cells, while the exopolysaccharide material was absent around the non-viscous form of S. iniae. The viscous type strains were disclosed more virulent than those of non-viscous type for olive flounder. The viscous strains were resistant to normal serum killing activity, while the non-viscous strains were susceptible to bactericidal activity of normal serum from olive flounder. The viscous strains were more resistant to opsonophagocytosis and decreased the chemiluminescence response of head kidney macrophages of olive flounder. All of the tested non-viscous S. iniae strains were efficiently destroyed by the macrophage after phagocytosis. Both the viscous and the non-viscous strains invaded and replicated in cultured fish cell line (CHSE-2l4). This cellular invasion may contribute to the pathogenesis of invasive S. iniae infection.

Synthesis of splinter-type and plate-type potassium titanate as reinforcements in brake pad for automobile (스플린터 및 판상형 티탄산칼륨염 합성 및 브레이크 보강재로서의 평가)

  • Kim, Sung-Hun;Kim, Jong-Young;Shim, Wooyoung;Lee, Jung Ju;Kwon, Sung Wook
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.29 no.6
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    • pp.329-337
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    • 2019
  • We synthesized potassium titanates having splinter and plate shape and evaluated frictional and wear properties of brake pad using them as reinforcements in friction materials. For splinter-shaped potassium titanates, potassium tetratitanate (K2O·4TiO2, PT4) with plate shape was prepared, then K ion of the titanate was leached by acid to make potassium hexatitanate (K2O·6TiO2, PT6), which was transformed to splinter-shaped PT6 by thermal treatment at 800℃. Plate-shaped potassium magnesium titanate (K0.8Mg0.4Ti1.6O4, PMT) was prepared by adding Mg in the potassium titanate using KCl as a flux. Using PT6 and PMT as reinforcements in friction materials of brake pad, we evaluated frictional and wear properties using 1/5-scale dynamometer. According to dynamometer test results, both reinforcements shows similar friction coefficient and fade & recovery behavior to conventional material and plate-shaped PMT exhibits higher wear resistance than splinter-shaped PT6.

Printability of coating layer with nano silica sol for inkjet printing high-end photo paper (나노 실리카 졸을 이용한 잉크젯 프린팅용 고품질 인쇄용지 도공층의 인쇄적성)

  • Kim, Hye-Jin;Nahm, Sahn;Han, Kyu-Sung;Hwang, Kwang-Taek;Kim, Jin-Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.29 no.6
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    • pp.352-358
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    • 2019
  • In recent years, printing paper with a function of information delivery and aesthetic value has attracted a great attention with increasing market demand for coated paper that is capable of high quality printing. The coated paper for inkjet printing with high-quality of photorealistic grades requires the coating layer with a good wettability and porous surface structure in order to improve the printability of ink. In this study, the coated paper was prepared using polyvinyl alcohol (PVA) and surface treated nano silica sol with silane coupling agent. It was confirmed that the coating layer with surface treated nano silica sol showed a uniform pore distribution and flat surface roughness. Glossiness of the prepared printing paper was similar to that of commercial high quality photo paper. Especially, the coated paper with surface treated nano silica sol showed improved printability with excellent roundness of the printed dot of ink. These results indicates that the coating layer with excellent wettability and uniform pore distribution can be formed by using the nano-silica particles with improved dispersibility through the surface treatment of the silane coupling agent.

Fracture strength of zirconia ceramic crowns according to tooth position (치아 부위에 따른 지르코니아 도재관의 파절강도)

  • Lee, In-Seob;Kim, Jeong-Mi;Dong, Jin-Keun
    • The Journal of Korean Academy of Prosthodontics
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    • v.48 no.2
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    • pp.94-100
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    • 2010
  • Purpose: The purpose of this study was to compare the fracture strength of the zirconia ceramic crowns according to tooth position. Material and methods: After 10 metal dies were made for each group, the zirconia ceramic crowns were fabricated using CAD/CAM system ($Lava^{TM}$ All-Ceramic System) and each crown was cemented on each metal die with resin cement (Rely $X^{TM}$ Unicem). The cemented zirconia ceramic crowns mounted on the testing jig were inclined with 30 degrees to the long axis of the tooth and the universal testing machine was used to measure the fracture strength. Results: 1. The fracture strength of the zirconia ceramic crown in the lower 1st molar (2963 N) had the highest and that in the lower central incisor (1035 N) had the lowest. 2. The fracture strength of zirconia ceramic crown was higher than that of the IPS Empress crowns in all tooth position. 3. The fracture mode of the crowns was similar. Most of fracture lines began at the loading area and extended through proximal surface perpendicular to the long axis of the crowns. 4. There were no significant differences on the fracture strength of the zirconia ceramic crowns according to tooth position except in premolar group. Conclusion: Within the limitations of this study, the results suggested that strength of zirconia ceramic crown is satisfactory for clinical use.

Alveolar ridge preservation with an open-healing approach using single-layer or double-layer coverage with collagen membranes

  • Choi, Ho-Keun;Cho, Hag-Yeon;Lee, Sung-Jo;Cho, In-Woo;Shin, Hyun-Seung;Koo, Ki-Tae;Lim, Hyun-Chang;Park, Jung-Chul
    • Journal of Periodontal and Implant Science
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    • v.47 no.6
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    • pp.372-380
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    • 2017
  • Purpose: The aim of this prospective pilot study was to compare alveolar ridge preservation (ARP) procedures with open-healing approach using a single-layer and a double-layer coverage with collagen membranes using radiographic and clinical analyses. Methods: Eleven molars from 9 healthy patients requiring extraction of the maxillary or mandibular posterior teeth were included and allocated into 2 groups. After tooth extraction, deproteinized bovine bone mineral mixed with 10% collagen was grafted into the socket and covered either with a double-layer of resorbable non-cross-linked collagen membranes (DL group, n=6) or with a single-layer (SL group, n=5). Primary closure was not obtained. Cone-beam computed tomography images were taken immediately after the ARP procedure and after a healing period of 4 months before implant placement. Radiographic measurements were made of the width and height changes of the alveolar ridge. Results: All sites healed without any complications, and dental implants were placed at all operated sites with acceptable initial stability. The measurements showed that the reductions in width at the level 1 mm apical from the alveolar crest (including the bone graft) were $-1.7{\pm}0.5mm$ in the SL group and $-1.8{\pm}0.4mm$ in the DL group, and the horizontal changes in the other areas were also similar in the DL and SL groups. The reductions in height were also comparable between groups. Conclusions: Within the limitations of this study, single-layer and double-layer coverage with collagen membranes after ARP failed to show substantial differences in the preservation of horizontal or vertical dimensions or in clinical healing. Thus, both approaches seem to be suitable for open-healing ridge preservation procedures.

Quality Characteristics and Antioxidant Activity of Buckwheat Soksungjang Prepared with Different Material Formula (원료 배합비를 달리하여 제조한 메밀 속성장의 이화학적 특성 및 항산화 활성)

  • Eom, Hyun-Ju;Kang, Hye Jeong;Park, Jung-Mi;Kim, Sang Hee;Song, In Gyu;Yoon, Hyang-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.8
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    • pp.1236-1241
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    • 2013
  • This study was conduct in order to provide information on formula optimization, salinity, and water content for commercialization of traditional buckwheat Soksungjang. To do this, we determined quality characteristics such as total microbial flora counts, amino-type nitrogen content, total polyphenol content, antioxidant activity, and performed a sensory evaluation. The moisture contents showed a gradual decrease during the fermentation period. There was no significant difference in all samples. During 70 days of fermentation, the pH showed a similar trend starting at a pH of 6, and dropping sharply to 5.5, except Experiment C (15% buckwheat powder in the meju, 10% NaCl, and 130% water in the total Soksungjang). On the other hand, total acidity increased regularly, however, its ranges were various points in all samples. The microbial population was reduced by 1~3 log cycle in all samples during the fermentation period. The content of amino-type nitrogen showed a gradual increase during fermentation, ranging from 160 to 213 mg% after 70 days. No remarkable difference in total polyphenol content and antioxidant activity was observed in all samples. In sensory evaluation, Soksungjang with 30% (control) and 15% (Experiment C) buckwheat showed a significantly higher overall score.

A preliminary study of the hydraulic-geometrical relations of bed slope in some selected alluvial rivers (우리 나라 沖積河川 河床傾斜의 水理機何 特性에 관한 연구)

  • ;;;Woo, Hyoseop;Yu, Kwonkyu;Park, Jongkwan
    • Journal of the Korean Geographical Society
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    • v.29 no.3
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    • pp.253-265
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    • 1994
  • The hydraulic-geometrica1 relations between the riverbed slope and water discharge and other hydraulic variables in some selected alluvial rivels in Korea have been investigated. The rivers from which the data relevant to this study were collected are mainly the first tributaries, considered to be mostly in the equilibrium state, of the 10 major rivers in Korea. The investigating methods adopted in this study are similar to the one suggested by Leopold and Maddock and the one suggested by Garde. All of 18 rivers their drainage areas of which range between 100-2,000 $\textrm{km}^2$ were considered and the changes in riverbed slope, drainage area, bed material size along the downstream river distance were measured. It is found in this study that the change in the riverbed slope, S, along the downstream can be expressed in terms of the coefficient, $\beta$, expressing the change in the drainage area along the downstream and the drainage area, A, by an empirical relation as 0.0063 0.0063 S = $S_{ 0}$ $A_{0}$$^{-------- +0.51}$A-$^{-------- -0.51}$. $\beta$ $\beta$ According to this relation, the riverbed slope of the river reaches investigated in this study appear to be proportional to the -0.6th power of the drainage area. This result is consistent with the previous ones obtained by Hack.k.

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Comparison of Fecal Microbiota of Mongolian and Thoroughbred Horses by High-throughput Sequencing of the V4 Region of the 16S rRNA Gene

  • Zhao, Yiping;Li, Bei;Bai, Dongyi;Huang, Jinlong;Shiraigo, Wunierfu;Yang, Lihua;Zhao, Qinan;Ren, Xiujuan;Wu, Jing;Bao, Wuyundalai;Dugarjaviin, Manglai
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.9
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    • pp.1345-1352
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    • 2016
  • The hindgut of horses is an anaerobic fermentative chamber for a complex and dynamic microbial population, which plays a critical role in health and energy requirements. Research on the gut microbiota of Mongolian horses has not been reported until now as far as we know. Mongolian horse is a major local breed in China. We performed high-throughput sequencing of the 16S rRNA genes V4 hypervariable regions from gut fecal material to characterize the gut microbiota of Mongolian horses and compare them to the microbiota in Thoroughbred horses. Fourteen Mongolian and 19 Thoroughbred horses were used in the study. A total of 593,678 sequence reads were obtained from 33 samples analyzed, which were found to belong to 16 phyla and 75 genera. The bacterial community compositions were similar for the two breeds. Firmicutes (56% in Mongolian horses and 53% in Thoroughbred horses) and Bacteroidetes (33% and 32% respectively) were the most abundant and predominant phyla followed by Spirochaete, Verrucomicrobia, Proteobacteria, and Fibrobacteres. Of these 16 phyla, five (Synergistetes, Planctomycetes, Proteobacteria, TM7, and Chloroflexi) were significantly different (p<0.05) between the two breeds. At the genus level, Treponema was the most abundant genus (43% in Mongolian horses vs 29% in Thoroughbred horses), followed by Ruminococcus, Roseburia, Pseudobutyrivibrio, and Anaeroplasma, which were detected in higher distribution proportion in Mongolian horses than in Thoroughbred horses. In contrast, Oscillibacter, Fibrobacter, Methanocorpusculum, and Succinivibrio levels were lower in Mongolian horses. Among 75 genera, 30 genera were significantly different (p<0.05) between the two breeds. We found that the environment was one of very important factors that influenced horse gut microbiota. These findings provide novel information about the gut microbiota of Mongolian horses and a foundation for future investigations of gut bacterial factors that may influence the development and progression of gastrointestinal disease in horses.

Comparative Study on the Bond Strength between Direct Tensile Test and Indirect Tensile Test for Bonded Concrete Overlay (직접인장 및 간접인장 실험방법에 따른 접착식 콘크리트 덧씌우기의 부착강도 비교 고찰)

  • Kim, Young Kyu;Lee, Seung Woo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.3
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    • pp.1153-1163
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    • 2013
  • Bonded concrete overlay is a favorable maintenance method since the material properties are similar to existing concrete pavements. In addition, bonded concrete overlay has advantage of structural performance based on being bonded together, both for the overlay layer and the existing pavement which perform as one monolithic layer. Therefore, it is important to have a suitable bond strength criteria for long term performance of bonded concrete overlay. This study aimed to investigate the affecting of bond strength on various bond characteristics, and to compare the bond strength between direct tensile test and indirect tensile test due to various conditions such as overlay materials, compressive and flexure strength of existing pavement, and deterioration status of existing pavement. As a result of this study, bond strength occurred by both of direct and indirect tensile test due to monotonic load is highly correlated such as coefficient of determination of 0.75 and P-value of 0.002. However, bond strength by indirect tensile test was relatively higher than bond strength by direct tensile test. It was known that correlation between direct and indirect tensile test was possible to use the characteristics analysis of bond fatigue behavior based on bond strength due to cyclic load which can simulate real field behavior of bonded concrete overlay.