• Journal of the Korean Wood Science and Technology
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• v.31 no.1
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• pp.16-21
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• 2003

The methanol extractives from root bark of Ulmus davidiana var japonica nakai were fractionated with n-hexane, ethyl ether, ethyl acetate and waster, the water soluble fraction was also fractionated with silicagel column chromatograhy. The chemical structure of purifided compounds were identified with UV, IR, ¹H-NMR and ¹³C-NMR spectra and the antibacterial activities also were investigated. Two different antibacterial compounds (compound A and B) were fractionated with silicagel chromatography and TLC. Compounds B was identified as a catechin rahmnoside. The both of compounds had antibacterial activity on Staphylococcus aureus and Salmonella typhimurium.

• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.501-505
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• 1990

Streptomyces sp. KISl3 isolated from soil was found to produce low molecular weight thiol protease inhibitors. The protease inhibitor production was closely linked to the cell growth and regulated by growth condition. The inhibitor was purified from the culture broth through butanol extraction, silicagel 60 column chromatography, Sephadex LH-20 gel filtration and preparative HPLC. The inhibitor showed specific inhibitory activity to thiol protease such as papain, picin and bromelain. The mode of inhibition against papain to Hammersten casein as a substrate was non-competitive.

• Journal of Environmental Health Sciences
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• v.2 no.1
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• pp.5-9
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• 1975

For analysis of Xanthene dyes according to the developing solvent and adsorbent was applied to Thin-layer chromatography with silicagel and cellulose plate. Silicagel chromato-plate used were prepared under different condition of activation. Using eight developing solvent, the influence of the condition for activation upon the separation of Xanthene dyes was investigated. The results are shown in Table 3. Methyl ethyl ketone+Acetone+$H_2O$ (10:0.1:0.4) mixture and n-butanol+Ammonia water (4:1) mixture gave clear separation for Xanthene dyes, including Fluorescein, Erythrosine Rhodamin B, Eosine, Rose bengale, phloxine and Acid red those Rf values decrease in the described ordor. Methyl ethyl ketone+Acetone+$H_2O$ (10:0.1:0.4) was applied to two adsorbents which were purchased from different manufactures. The results of Chromatograms are obtained Figure 6.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.249-254
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• 1997

From Korean cultivated peony root, paeoniflorin and albiflorin, which are generally considered to be principal components of peony root, were isolated by silicagel column chromatography. Their structures were identified by spectroscopic methods $(UV,\;FT-IR,\;^1H{\cdot}^{13}C-NMR)$ and their purities were 98% and 93%, respectively. The concentrations of paeoniflorin and albiflorin in ten Korean cultivated peony lines were determined by reverse phase HPLC. The concentrations of paeoniflorin ranged from 1. 56 to 4.04% and those of albiflorin ranged from 0.04 to 1. 98% in ten Korean cultivated lines. In the ten cultivated lines, the concentrations of albiflorin in Punggi lines were higher than other lines.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.199-201
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• 2005

This paper describes a simple procedure for the quantitative analysis of 7 PCBs (polychlorinated biphenyls) in soils on the waste reclaimed land, The procedure involved sample clean up using silicagel column, acetonitrile partition and sulfuric acid procedures. The instrumental technique is applied GC/PDD(gas chromatography/pulsed discharge detector) and GC/ECD(gas chromatography/electron capture detector). Concentration of $sub-{\mu}g/g$ level was attainable with 20g soils on the waste reclaimed land.

• Korean Journal of Pharmacognosy
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• v.6 no.3
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• pp.149-150
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• 1975

Silicagel colum chromatography of the ether extract of the fruits of Angelica decursiva $F_R$. et SAV. gave four crystalline products of coumarins and one steroid. The following furocoumarins and ${\beta}-sitosterol$ were identifed by UV, IR, NMR spectra and physicochemical tests: ${\beta}-sitosterol$, iso-imperatorin, imperatorin, bergapten, umbelliferone.

• Proceedings of the Ginseng society Conference
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• 1974.09a
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• pp.45-48
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• 1974

[ $\beta$-Sitosterol and oleanolic acid were isolated in a pure form from Radix Panax Ginseng, the genuine Ginseng drug, by column chromatography on Silicagel and aluminium oxide (Woelm). TLC indicates the presence of at least three other triterpene sapogenins.

• Microbiology and Biotechnology Letters
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• v.17 no.2
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• pp.121-125
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• 1989

Actinomycetes GF 155-2, which produced an extracellular pepsin inhibitor, was isolated from soil samples. Optimal conditions of inhibitor production by flask-shacking culture were 2% glucose, 0.7% polypeptone, initial pH 1.0, culture time 60 hours and temperature 30%. Effect of in-organic salts was not observed. About 5mg of colorless crystalline inhibitor was obtained from 5L culture broth in jar tormentor by means of ammonium sulfate precipitation, methanol extraction, and column chromatographies on Amberlite IR-120, XAD-2 and silicagel 60.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.16 no.1
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• pp.40-46
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• 1990

A method was described for the analysis of Artemisia extract in cosmetic soap. Esculetin-6-methylether was used as indicator ingredient for analysis of Artemisia extract. It was isolated from the plant and purified with silicagel column. The structure was conformed with IR, NMR and MASS spectroscopy. The Artemisia extract in sample was isolated with chloroform and interfering com- pounds were eliminated with silicagel column. The Artemisia extract was determined by reverse phase high- performance liquid chromatography with a fluorescence detector and a solvent system of H2O/THF/MeOH. The recorveries of Artemisia extract were 93.9-106.1% in the cosmetic soap.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.215.2-216
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• 2003

Velutin and betulinic acid were isolated as a cytotoxic principle from the dichloromethane extract of Korean mistletoe (Viscum album var. coloratum) by repeated silicagel chromatography and recrystallization. In in vitro analysis of cytotoxic activity using NIH-3T3 cells, dichloromethane extract of Korean mistletoe was shown to be highly cytotoxic against tumor cells. And we obtained active compound (reffered to VD-2 and VD-6) from the dichloromethane extract. (omitted)