Analysis of Medicinal Plants in Cosmetics(II) The HPLC Separation and Quantitation of Artemisia Extract in Cosmetic SOAP

화장품중 생약성분의 분석(II) 화장비누중의 쑥추출액의 함량분석

A method was described for the analysis of Artemisia extract in cosmetic soap. Esculetin-6-methylether was used as indicator ingredient for analysis of Artemisia extract. It was isolated from the plant and purified with silicagel column. The structure was conformed with IR, NMR and MASS spectroscopy. The Artemisia extract in sample was isolated with chloroform and interfering com- pounds were eliminated with silicagel column. The Artemisia extract was determined by reverse phase high- performance liquid chromatography with a fluorescence detector and a solvent system of H2O/THF/MeOH. The recorveries of Artemisia extract were 93.9-106.1% in the cosmetic soap.