• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.231-231
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• 2017

Drought limits rice production under upland condition. This study quantified the effect of rhizobacteria inoculation on rice root system developmental response to drought and its role in maintaining high soil water use, and dry matter production under drought using NSIC Rc192 (rainfed lowland rice variety). The source of inoculant was Streptomyces mutabilis, a recently isolated rhizobacteria containing plant growth promoting compounds such as ACC deaminase, indole-3-acetic acid and phosphatase (Cruz et al., 2014, 2015). In the first experiment, pre-germination inoculation of seeds with S. mutabilis significantly increased the shoot and root (radicle) length as well as root hair lengths, relative to the non-inoculated control. In the second experiment, rice plants inoculated with S. mutabilis and grown in rootbox with soil generally had greater total root length under drought regardless of the timing of inoculations, relative to the non-inoculated control. Consequently, improved root system development contributed to the increase in soil water uptake under drought and thus, dry matter production. Among inoculation treatments, one-time inoculation of S. mutabilis either at pre-germination or pre-drought stress at 14 days after sowing (DAS), had significantly greater shoot dry matter production than three-time inoculation at pre-germination, at thinning (3 DAS) and at pre-drought (14 DAS). This study demonstrated the effectiveness of rhizobacteria (S. mutabilis) containing growth promoting compounds for enhancing drought dehydration avoidance root traits and improving the growth of rice plants under drought condition.

• 한국약용작물학회지
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• 제11권4호
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• pp.289-297
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• 2003

Using Agrobacterium-me야ated transformation method the auxin-regulated cotton GST (Gh-5) constructs were used to transform Rehmannia glutinosa L. The PCR analysis was conducted to verify transgenicity. Based on the PCR analysis, there was verified that the 988 bp DNA band had showed in transgenic plant genomes in PCR anaJysis using Gh5-1 and Gh5-2 primers. The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of Chinese foxglove (Rehmannia glutinosa L.) were investigated. Factors such as cocultivation period, use of acetosyringone, postcultivation in darkness, and different kanamycin concentrations for selection were assessed. In vitro regeneration, the number of leaves, shoot lengths and numbers on MS medium were superior to on B5 and WPM medium, and the shoot formation rate was highest level of 95% in cultured base part containing leaf stalk. Addition of acetosyringone at concentration of $200{\mu}M$ to cocultivation medium and 3-day of cocultivation improved transformation frequencies. Exposure of explants to darkness for 4 weeks on selection medium resulted in further increased the regeneration frequency of transgenic shoots. In PCR analysis, the amplified fragments of Gh5 gene were detected (988 bp), and GST-expressing transgenic R. glutinosa L. plants had approximately three-fold higher activity in leaf extracts compared with control plant.

• 생물환경조절학회지
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• 제20권4호
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• pp.341-345
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• 2011

이 연구는 화단용 국화의 식재 효율성을 높이기 위한 목적으로 기존에 개발된 식생매트에 삽수를 이용하여 최적의 매트조건을 구명하고자 수행하였다. 8가지 처리 조건의 매트에 Dendranthema grandiflorum 'Ford'를 삽목하고 난 후 50일 후 생육을 조사한 결과, 매트 내 뿌리 분포는 10mm 두께의 중간에 배양토를 삽입한 coir net(C 처리)에서 가장 좋았고, 초장과 지상부 생체중도 각각 29cm, 5.6g으로 동일 조건에서 가장 높은 값을 나타냈다. 뿌리가 형성된 매트를 포장에 정식 40일 후 초장은 12mm 두께의 중간에 배양토를 삽입한 Jute net가 가장 좋았으나, 지상부 생체중은 C 처리와 차이가 없었다. 매트 내 뿌리 형성과 정식 후 삽수 길이별 생육은 길이 5cm인 삽수가 3과 8cm인 것 보다 좋은 결과를 나타냈다.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.883-888
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• 2012

The efficacy of cowdung, Bangladesh Institute of Nuclear Agriculture (BINA)-biofertilizer, and Bangladesh Agricultural University (BAU)-biofungicide, alone or in combination, was evaluated for controlling foot rot disease of lentil. The results exhibited that BINA-biofertilizer and BAU-biofungicide (peat soil-based Rhizobium leguminosarum and black gram bran-based Trichoderma harzianum) are compatible and have combined effects in controlling the pathogenic fungi Fusarium oxysporum and Sclerotium rolfsii, which cause the root rot of lentil. Cowdung mixing with soil (at 5 t/ha) during final land preparation and seed coating with BINA-biofertilizer and BAU-biofungicide (at 2.5% of seed weight) before sowing recorded 81.50% field emergence of lentil, which showed up to 19.85% higher field emergence over the control. Post-emergence deaths of plants due to foot rot disease were significantly reduced after combined seed treatment with BINA-biofertilizer and BAU-biofungicide. Among the treatments used, only BAU-biofungicide as the seed treating agent resulted in higher plant stand (84.82%). Use of BINA-biofertilizer and BAU-biofungicide as seed treating biocontrol agents and application of cowdung in the soil as an organic source of nutrient resulted in higher shoot and root lengths, and dry shoot and root weights of lentil. BINA-biofertilizer significantly increased the number of nodules per plant and nodules weight of lentil. Seeds treating with BAU-biofungicide and BINA-biofertilizer and soil amendment with cowdung increased the biomass production of lentil up to 75.56% over the control.

• Journal of Applied Biological Chemistry
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• 제62권3호
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• pp.247-250
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• 2019

Wheat is one of the most important grains. Its consumption is increasing globally. Many countries are making efforts to increase the extent of wheat harvest. It is known that plant growth promoting rhizobacteria (PGPRs) have beneficial effects on various plants. Two PGPRs including Paenibacillus pabuli strain P7S (PP7S) and Pseudomonas nitroreducens strain IHB (PnIHB) were employed to investigate effects of PGPRs on early growth of three wheat cultivars (Koso, Seakumkang, and Jokyung). While PP7S had adverse effects on Seakumkang and Jokyung, PP7S had positive effects on Koso except root length compared to control group having no treatment of PP7S. However, all treatments with PnIHB had adverse effects on germination rate, root/shoot lengths, vigor index, and dry root/shoot weights of all three wheat cultivars. These positive effects with PP7S on Koso might be related to the earlier emergence of wheat seed above soil which is known to be an indicator of increased yield. Results of the present study suggest that if proper PGPR strains are selected, they could have positive effects on early growth rate of a wheat cultivar.

• 한국환경과학회지
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• 제21권8호
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• pp.931-938
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• 2012

The effects were investigated of different lengths and numbers of slitwalls in drainless containers on growth and change in soil moisture volumes on the growth of Diffenbachia amoena 'Marianne' and Begonia rex. Drainless containers filled with amended soil, with square shape ($240mm{\times}240mm$) were used, as well as three different sets of slitwalls (2, 4 or 8, respectively) in addition to non-slitwall containers. Two indoor foliage plants were grown in slitwall containers in randomized blocks with 3 replications in greenhouse conditions, from March to September, 2009. Soil moisture volumes per container were measured by weighing containers every 2 hours during the day. The change in soil moisture volumes showed considerable differences among slitwalls tested in comparison to control containers before and after twice-weekly irrigation. Particularly, the differences in the S2 (195mm, slitwall 2) containers were significantly greater than other containers tested. For Diffenbachia amoena 'Marianne', plant height, length of leaf, dry weight and fresh weight were higher with S2 containers than with those grown in other containers tested. The Begonia rex with the best quality in terms of plant height, length of leaf and width of leaf was grown in S8 (360mm, slitwall 8) containers. Particularly, statistical analysis has indicated that shoot fresh weights of Begonia rex grown in S8 were 3-fold higher than those grown in CS8 containers. The different results obtained within the two species led us to hypothesize a species-specific influence on indoor foliage plant performance. However, plants of both species grown in slitwall containers showed good results compared with plants grown in non-slitwall containers.

• 농업과학연구
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• 제45권2호
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• pp.143-153
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• 2018

Soil characteristics along with various container lengths have an important role in the early survival rate and growth of seedlings by influencing the seedling quality. This experiment was conducted to investigate the effect of container length and different soil mixtures on the growth of poplar in a greenhouse. Two types of soil, homogeneous vs. heterogeneous, were used along with two container lengths (30 vs. 60 cm). The heterogeneous soil was made by dividing 50% vermiculite from a mixture of 25% vermicompost and 25% nursery soil in volume. For the homogeneous soil, the above three soil types were mixed together. Populus euramericana clone cuttings were planted in late April, and then, the growth height, root collar diameter (RCD) and biomass were measured in August. The height of the poplar was not significantly affected by container length and soil type, but the RCD was significantly affected by soil type. Leaf and root biomass was higher at the long container than at the short container for both soil treatments, but stem biomass was lower at the heterogeneous soil than at the homogeneous soil treatment. Root to shoot biomass ratio was higher at the heterogeneous soil treatment than at the homogeneous soil treatment by 12%. In conclusion, heterogeneous soil along with a long container is suitable to increase the carbon allocation into the root.

• 원예과학기술지
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• 제32권4호
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• pp.421-426
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• 2014

본 연구에서는 '천중도백도' 복숭아(Prunus persica)의 고품질 다수확 생산을 하는데 적합한 결과지를 구명하기 위하 여 몇 가지 결과지 방향 및 굵기 처리에 따른 신초장, 과중, 당도 등의 변화를 조사하였다. 각 조사항목의 평균치를 보면 결과지 직경의 경우 6.9mm, 신초장은 32.6cm, 과중은 333.6g, 당도는 $11.2^{\circ}Brix$였으며, 결과지 직경과 신초장은 변이계수가 각각 35.8%와 75.3%로 높았다. 254개 결과지 및 과실 특성 중 직경 9.0mm 이하 결과지가 76.4%, 40cm이하 신초가 70.0%, 무게가 310-340g 과실이 24.8%, 그리고 당도가 $10.5-12.5^{\circ}Brix$인 과실이 66.9%를 차지하였다. 신초장은 결과지 방향에 관계없이 결과지 굵기의 영향을 크게 받았고, 과중은 결과지 방향 및 굵기의 상호작용의 영향을 크게 받았다. 그리고 당도는 결과지 방향에 관계없이 결과지 굵기의 영향을 크게 받았다. 결과지 직경과 과중간 회귀 분석 결과 하향 결과지에서는 정의 상관($r^2=0.246^{**}$)을 보였고 상향 결과지에서는 부의 상관($r^2=0.094^*$)을 보였다. 따라서 고품질 '천중도백도' 과실 생산을 위해서는 하향이 면서 직경이 9.0mm 이하인 결과지를 활용하는 것이 좋을 것으로 판단된다.

• The Korean Journal of Ecology
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• 제27권1호
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• pp.49-55
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• 2004

해안사구 식물인 통보리사초에 대해 생육 특성을 규명하기 위해 충청남도 태안군 원북면 신두리 해안 사구에서 2001년 4월부터 2003년 6월까지 통보리사초의 지상부와 지하부를 조사하였다. 그 결과, 해안사구에서 통보리사초는 갯그령 다음으로 만조선에 가까이 출현하는 식물종이었으며 밀도는 퇴적이 일어나는 지역에서 150개체/㎡으로 가장 높았다. 그리고 개화율은 모래 퇴적지역에서 70% 이상을 유지하였으나 안정지역에서는 10% 이내이었다. 따라서 통보리사초는 모래의 퇴적지역에 적응력이 탁월한 식물종으로 나타났다. 그리고 지상부는 대단히 불규칙한 분포를 하였다. 모래의 퇴적지역과 안정지역에서 토양의 깊이별 지하경의 분포를 조사한 결과 전자에서는 깊이별로 큰 차이가 없었으나 후자에서는 대부분 20 ㎝ 이내에 위치하였다. 이러한 결과에 의하면 지하경은 퇴적지역에서는 수직방향으로, 안정지역에서는 수평방향으로 생장하는 것으로 판단할 수 있었다. 새로운 지하경을 발생시킬 수 있는 지하경 의 능력은 지표면으로부터 150 ㎝ 이하 혹은 5년 전의 것이었다. 새로 발생하는 지하경의 수와 평균길이는 전년도의 지상부 크기와 관계가 깊었다. 즉, 지상부의 건중량이 0.5 g 미만에서는 1개, 2.0∼2.5 g에서는 2개, 3 g 이상에서는 5개의 지하경을 각각 생산하였다. 한편,지하경의 길이는 10∼100 ㎝ 범위에 있었으며 전년도 지상부의 건중량이 0.5 g미만일 경우는 13.0 ㎝ 이었지만 3 g 이상의 경우 평균 57.6 ㎝로 약 4.4배에 달하였다.

• Journal of Microbiology
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• 제45권2호
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• pp.171-174
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• 2007

The growth stimulation of wild plants by several bacterial species showing plant growth-promoting capabilities was examined in a barren lakeside area at Lake Paro, Korea. Microbial numbers and activities in the field soil were monitored for 73 days after inoculation of the bacteria. The acridine orange direct counts for the total soil bacterial populations ranged between $2.0-2.3{\times}10^{9}\;cells/g$ soil and $1.4-1.8{\times}10^{9}\;cells/g$ soil in the inoculated and uninoculated soils, respectively. The numbers of Pseudomonas spp., which is known as a typical plant growth-promoting rhizobacteria, and the total microbial activity were higher in the inoculated soil compared to those in the uninoculated soil. The average shoot and root lengths of the wild plants grown in the inoculated soil were 17.3 cm and 12.4 cm, respectively, and longer than those of 11.4 cm and 8.5 cm in the uninoculated soil. The total dry weight of the harvested wild plants was also higher in the inoculated soil (42.0 g) compared to the uninoculated soil (35.1 g). The plant growth-promoting capabilities of the inoculated bacteria may be used for the rapid revegetation of barren or disturbed land, and as biofertilizer in agriculture.