• Journal of Microbiology and Biotechnology
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• v.30 no.2
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• pp.259-270
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• 2020

Listeria monocytogenes is a gram-positive, facultative anaerobe food pathogen responsible for the listeriosis that mostly occurs during the low-temperature storage of a cold cut or dairy products. To understand the systemic response to a wide range of growth temperatures, L. monocytogenes were cultivated at a different temperature from 10℃ to 42℃, then whole cell proteomic analysis has been performed both exponential and stationary cells. The specific growth rate increased proportionally with the increase in growth temperature. The maximum growth rate was observed at 37℃ and was maintained at 42℃. Global protein expression profiles mainly depended on the growth temperatures showing similar clusters between exponential and stationary phases. Expressed proteins were categorized by their belonging metabolic systems and then, evaluated the change of expression level in regard to the growth temperature and stages. DnaK, GroEL, GroES, GrpE, and CspB, which were the heat&cold shock response proteins, increased their expression with increasing the growth temperatures. In particular, GroES and CspB were expressed more than 100-fold than at low temperatures during the exponential phase. Meanwhile, CspL, another cold shock protein, overexpressed at a low temperature then exponentially decreased its expression to 65-folds. Chemotaxis protein CheV and flagella proteins were highly expressed at low temperatures and stationary phases. Housekeeping proteins maintained their expression levels constant regardless of growth temperature or growth phases. Most of the growth related proteins, which include central carbon catabolic enzymes, were highly expressed at 30℃ then decreased sharply at high growth temperatures.

• Journal of Korea Port Economic Association
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• v.24 no.1
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• pp.1-10
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• 2008

This paper analyses the dynamic causal relationship between vessel export and economic growth using annual data over the period from 1977 to 2006. Tests for ADF unit-roots, the dynamic vector using Johansen's multiple cointegration procedure, dynamic vector error correction model and impulse response function are presented. The findings of the Granger test suggest that vessel export Granger-causes economic growth in the short-run and economic growth Granger-causes exports in the short and long-run. The empirical results of impulse-response analysis show that the vessel export to a shock in real GDP responds positively and the real GDP responds positively to the shocks in vessel export. Also, the results indicate that the impact of vessel export shock on the real GDP is short-lived.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.7
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• pp.777-787
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• 2012

The transient analysis for the output voltage of a piezoresistive microaccelerometer takes a relatively high computation time because at least two iterations are required to calculate the piezoresistive-structural coupled response at each time step. In this study, the high computational cost for calculating the transient output voltage is considerably reduced by an approach integrating the mode superposition method and the least square method. In the approach, data on static displacement and output voltage calculated by piezoresistive-structural coupled simulation for three acceleration inputs are used to develop a quadratic regression model, relating the output voltage to the displacement at a certain observation point. The transient output voltage is then approximated by a regression model using the displacement response cheaply calculated by the mode superposition method. A high-impact microaccelerometer subject to several types of acceleration inputs such as 100,000 G shock, sine, step, and square pulses are adopted as a numerical example to represent the efficiency and accuracy of the suggested approach.

• Knowledge Management Research
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• v.19 no.2
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• pp.1-19
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• 2018

This study aims to identify the causal relationship among public technology transfer, technological performance, and research and development (R&D) productivity. Using the impulse-response function(IRF) of a panel vector autoregressive model (panel VAR), this study suggests the results of how long the factors such as technological performance (patent), public technology transfer, and R&D productivity takes and lasts if a one-unit shock of standard deviation occurs. As a result, first, the increase of public technology transfer activities has no power to increase the technology performance but improve the R&D productivity. If the public institute increases its technology transfer activities by one unit, the R&D productivity will increase within five years. Second, the impact of increasing technological performance on improvement of public technology transfer and R&D productivity is an insignificant. Third, the effect of R&D productivity on the public technology transfer creates a substantial reaction after a current time. Considering the structural relationships among public technology transfer, technological performance, and R&D productivity, if policy makers intend to construct the active R&D circumstance, technology suppliers should be motivated to run the active R&D mechanism because they achieve gains.

• Structural Engineering and Mechanics
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• v.77 no.3
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• pp.305-314
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• 2021

In order to reduce enormous cost of real-scale underwater explosion experiments on ships, the mechanical response of the ships have been analyzed by combining scaled-down experiments and Hopkinson's scaling law. However, the Hopkinson's scaling law is applicable only if all variables vary in an identical ratio; for example, thickness of ship, size of explosive, and distance between the explosive and the ship should vary with same ratio. Unfortunately, it is infeasible to meet such uniform scaling requirement because of environmental conditions and limitations in manufacturing scaled model systems. For the facile application of the scaling analysis, we propose a generalized scaling law that is applicable for non-uniform scaling cases in which different parts of the experiments are scaled in different ratios compared to the real-scale experiments. In order to establish such a generalized scaling law, we conducted a parametric study based on numerical simulations, and validated it with experiments and simulations. This study confirms that the initial peak value of response variables in a real-scale experiment can be predicted even when we perform a scaled experiment composed of different scaling ratios for each experimental variable.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.70-77
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• 1999

An extremely cadmium-tolerant budding yeast, Hansenula anomala B-7 underwent a morphological switch in response to either heat shock treatment or cadmium stress, respectively. It exhibited a morphological transition from a unicellular yeast form to a pseudohyphae-like coagulation when subjected to prolonged heat shock treatment. In contrast, the yeast cells showed an irregularity in surface morphology when given thermal stress for a short time. Patterns of proteins expressed in the pseudohyphae-like cells demonstrated that several proteins were overexpressed while others were underexpressed in comparison with those prepared from the cells in the yeast form. It was a striking feature, however, that nearly 40％ of the proteins extracted from the cells in the pseudohyphae form appeared to be composed of a single polypeptide. This polypeptide was apparently overexpressed during the pseudohyphae phase and its molecular weight was estimated to be 58 kDa according to SDS-PAGE analysis. However, a significant level of the protein was not observed in the cells before transition to pseudohyphae. The architecture of the cell shape was also damaged when incubated in a medium containing more than 1,000 ppm (8.9mM) of cadmium ions, although able to proliferate at a slow rate. However, the irregularity in the cell morphology exerted either by the brief heat shock treatment or by the cadmium stress with the high concentrations of the metal ions was not repaired, even though the damaged cells were allowed to grow for sufficient time in fresh, cadmium-free medium.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.799-804
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• 2004

Dynamic characteristic of CD/DVD deck for vehicle is analyzed in this paper. Generally CD/DVD deck for vehicle is used from inferior environment with the vibration of the vehicle and shock of outside and so on. Therefore it must have the structure which is stabilized from the vibration to prevent read error. For this purpose, vibration characteristic of the deck for vehicle should be identified. To analyze characteristic of the deck system, we perform the signal analysis and modal testing using the FFT analyzer. Also we change the design factor degrading the performance of the deck system and verify the efficiency improvement using the acceleration measurement occurring to the sound discontinuation.

• Computers and Concrete
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• v.6 no.5
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• pp.377-390
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• 2009

In this paper an improved one-dimensional frame element for modelling of reinforced concrete beams and columns subjected to impact is presented. The model is developed in the framework of a flexibility fibre element formulation that ignores the shear effect at material level. However, a simple shear cap is introduced at section level to take account of possible shear failure. The effect of strain rate at the fibre level is taken into account by using the dynamic increase factor (DIF) concept for steel and concrete. The capability of the formulation for estimating the element response history is demonstrated by some numerical examples and it is shown that the developed 1D element has the potential to be used for dynamic analysis of large framed structures subjected to impact of air blast and rigid objects.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.29 no.7 s.238
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• pp.1035-1041
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• 2005

Dynamic characteristics of CD/DVD deck fur vehicle are analyzed in this paper. Generally CD/DVD deck for vehicle is used from inferior environment with the vibration of the vehicle, shock of outside and so on. Therefore it must have the structure which is stabilized from the vibration to prevent read error. For this purpose, vibration characteristic of the deck for vehicle should be identified. To analyze characteristic of the deck system, we perform the signal analysis and modal testing using the EFT analyzer. Also we change the design factor degrading the performance of the deck system and verify the efficiency improvement using the acceleration measurement occurring to the sound discontinuation.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.33 no.7
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• pp.33-39
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• 2005

A mathematical model, GUNBLAST, of blast waves emitted from a gun muzzle is established, and structural response analyses for the blast load are performed. The blast wave can be divided into two kinds of waves, free field and reflected blast waves. In this research, the free field blast wave model is established by the use of a scaling approach, and the reflected blast wave is calculated by using the oblique shock theory and computational fluid dynamic calculation. GUNBLAST is applied to two kinds of structural models. To investigate the effect of the muzzle distance from a structural surface, the blast waves on a plate for various muzzle distances are compared to uniform loads. Moreover, the transient response analysis of an aircraft wing model with a 12.7mm gun is carried out by using MSC/NASTRAN. From the results, it can be shown that the blast wave can cause broad random vibration and high frequency damage to equipments mounted in the aircraft.