• Journal of Life Science
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• v.9 no.4
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• pp.408-413
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• 1999

Extracellular capsidiol, sesquiterpenoid phytoalexin, in the medium of pepper (Capsicum annuum L.) suspension cells was not identified from control cells, but highly accumulated in the elicitor-induced cells within 6 hours after the addition of 0.05$\mu\textrm{g}$/$m\ell$ cellulase. Capsidiol production in elicitor-induced cells was markedly suppressed by cytochrome P450 inhibitors, such as ancymidol and ketoconazole demonstrating that biosynthesis of capsidiol is catalyzed by at least on hydroxylation enzyme in the biochemical pathway. Based on protein electrophoresis, two bands, 23.0kDa and 27.5kDa, were identified as newly synthesized polypeptides in the elicitor-induced suspension cells, suggesting that pepper cells which were subjected to elicitor treatment activate specific gene(s) for capsidiol biosynthesis in cultured cells.

• Proceedings of the Korean Society of Life Science Conference
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• 2001.09a
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• pp.30-30
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• 2001

• Korean Journal of Plant Tissue Culture
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• v.28 no.5
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• pp.249-254
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• 2001

Effects of ultraviolet stress and elicitors, cellulase and jasmonic acid (JA), for the production of capsidiol, sesquiterpenoid phytoalexin, in seedlings and suspension cultures of pepper (Capsicum annum L. cv, Soobicho) were examined. Extracellular capsidiol in the medium of suspension cultures was absent from control cells, but accumulated in the elicitor treated cells with 0.05 $\mu\textrm{g}$/mL of cellulase or 0.1 $\mu\textrm{g}$/mL JA. Elicited cells gradually decreased their viability and eventually died within 48 hours of elicitor treatment by the toxicity of capsidiol accumulated in the culture medium. Capsidiol production in the leaves of pepper seedlings was markedly increased by the treatment of ultraviolet stress and reached maximum level at 48 hours of irradiation. Infiltration of elicitors, 0.05 $\mu\textrm{g}$/mL cellulase or 1.0 $\mu\textrm{g}$/mL JA, to the surface of leaf or fruit, stimulated the elicitation of the cells which resulted in the production of capsidiol and expansion of pathogene-like lesion around the elicitor treated region.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.279-284
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• 1997

The extracellular sesquiterpenoids were accumulated in cell and hairy root cultures of Hyoscyamus muticus by elicitation using extracts of Rhizoctonia solani. The vetispiradiene synthase (VS) which is the first committed step in biosynthetic pathway leading to formation of solavetivone, lubimin, and rishitin from isoprenoid intermediate farnesyl pyrophosphate was induced upon elicitation, whereas no sesquiterpenoids and VS activity were detected in both control cell and hairy root cultures. VS activity increased rapidly and reached its maximum 12 h in both cell and hairy root cultures upon elicitor treatment. VS activities were paralleled with the absolute levels of VS polypeptide(s). Interestingly, the profiles of sesquiterpenoid accumulation in hairy root cultures were different from those in cell cultures. The hairy root culture seemed to fail to metabolize solavetivone further to lubimin.

• Korean Journal Plant Pathology
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• v.12 no.3
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• pp.285-290
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• 1996

3-hydroxy-3-methyglutaryl coenzyme A reductase (HMGR)는 phytoalexin을 포함하는 수 많은 isoprenoid화합물의 생합성을 조절하는 효소이다. 토마토의 경우 sesquiterpenoid phytoalexin류가 식물방어를 위한 반응산물로서 축적되는 것이 알려져 있다. Verticil-lium albo-atrum이나 Fusarium oxysporum으로부터 추출한 elicitor를 토마토의 배양세포에 처리하는 경우 처리량의 증가에 따른 2.7kb 크기의 HMGR mRNA의 상당한 유도증가가 토마토의 HMG2 DNA를 이용한 northern hybridization에 의해 관찰되었다. 토마토의 잎, 뿌리, 줄기 등에 기계적 상해를 가하는 경우에서도 HMGR mRNA는 2단계를 걸쳐 증가함이 관찰되었다. HMGR mRNA는 양 실험의 경우 모두 9시간에서 12시간 사이에서 최대발현됨이 관찰되었다.