Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Elicitor-InduciblePhytoalexin from Cell Suspension Cultures of Pepper(Capsicum annuum L.)

고추(Capsicum annuum L.) 배양세포의 Elicitor 유도성 Phytoalexin 생성

  • 권순태 (안동대학교 생명자원과학부) ;
  • 오세명 (안동대학교 생명자원과학부)
  • Published : 1999.08.01
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Abstract

Extracellular capsidiol, sesquiterpenoid phytoalexin, in the medium of pepper (Capsicum annuum L.) suspension cells was not identified from control cells, but highly accumulated in the elicitor-induced cells within 6 hours after the addition of 0.05$\mu\textrm{g}$/$m\ell$ cellulase. Capsidiol production in elicitor-induced cells was markedly suppressed by cytochrome P450 inhibitors, such as ancymidol and ketoconazole demonstrating that biosynthesis of capsidiol is catalyzed by at least on hydroxylation enzyme in the biochemical pathway. Based on protein electrophoresis, two bands, 23.0kDa and 27.5kDa, were identified as newly synthesized polypeptides in the elicitor-induced suspension cells, suggesting that pepper cells which were subjected to elicitor treatment activate specific gene(s) for capsidiol biosynthesis in cultured cells.

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References

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