• 대한수의학회지
• /
• 제32권4호
• /
• pp.641-647
• /
• 1992

This study was conducted to determine the serum antibodies against toxoplasma in the artificially infected dogs, pet and street dogs by latex agglutination (LA) and indirect fluorescent antibody (IFA) test. LA test was carried out with commercial Toxo-MT kit (Eiken chemical Co.) and IFA test was carried out with rabbit-anti-dog IgG labelled with FITC (Cappel Co.) and toxo-antigen slides prepared in laboratory. The results obtained were as follows ; 1. Antibodies to Toxoplasma gondii in the artificially infected dogs were detected firstly at the Day 8 in IFA and Day 9 in LA test after inoculation. Positive antibody reactions by these tests were declined gradually afterward but maintained up to 12 weeks. 2. In LA test serum antibody titers in 310 test sera were shown as 10 cases(32%) in 1 : 32.5(1.0%) in 1 : 64, 4(1.3%) in 1 : 128 and 2(0.7%) in 1 : 256. In IFA test serum antibody titers 310 test sera were shown as 17 cases(5.5%) in 1 : 64, 8(2.6%) in 1 : 128 and 5(1.6%) in 1 : 256. 3. In the total of 310 sera from pet and street dogs toxoplasma antibody positive rates were 21 cases (6.8%) by LA and 30 cases (9.7%) by IFA test and the positive detection rates between these two groups by LA and IFA test were not significant(p<0.05). 4. In the total of 115 sera from pet dogs toxoplasma antibody positive rates were 12 cases(10.4%) by LA and 15(13.0%) by IFA test. And in the 195 street dogs the positive rates were 9 cases(4.6%) by LA and 15(7.7%) by IFA test. Also, the positive detection rates between these two groups by LA and IFA test were not significant(p<0.05). 5. Agreement of reactivity between LA and IFA test for 310 sera was 91.3% in total of 283 cases consisting of 12 cases(3.9%) of both LA and IFA positive and 271 cases(87.4%) of LA and IFA negative. 6. LA test was almostly equivalent to the IFA test in producibility and proved to be a simple tool for the screening of toxoplasma antibody in laboratory.

• 한국동물위생학회지
• /
• 제29권3호
• /
• pp.267-276
• /
• 2006

To investigate serum antibody titers of Newcastle disease (ND) virus, serum samples were collected from 19,774 slaughtered chickens from 862 farms in Geoje, Goseong, Tongyung and other province during the period from January 2001 to December 2005. The results obtained are summarized as follows; 1. The negative percentage of ND antibody titer with below 1.0 levels decreased from 46.7% in 2001 to 40.5% in 2004 but those of it increased to 47.0% in 2005 and the positive percentage of ND antibody titer with higher 5 levels increased from 15.8% in 2001 to 26.2% in 2005. 2. The season levels of ND antibody titer showed various levels according to seasons but the general trend of ND antibody titer decreased in the later half of the year than those of the first half of the year. 3. The negative percentage of ND antibody titer on provinces decreased from 52.0% (2001) to 36.2% (2004) in Goseong but those of it increased to 51.4% in 2005. The negative percentage of ND antibody titer of Tongyung and other provinces increased from 51.0%, 28.5% (2001) to 55.0% and 44.3% (2005), respectively. 4. The year average levels of ND antibody titer increased from 2.2 to 2.6 during 2001- 2004 but those of it decreased to 2.4 in 2005. The provinces average levels of ND antibody titer increased from 2.0 to 2.8 during 2001 - 2005 and others provinces except Gyeongnam decreased from 3.3 to 2.6 during the 2001-2005. 5. The farm average levels of ND antibody titer with below 1.0 levels decreased from 26.3% in 2001 to 10.2% in 2004 but those of it increased to 17.6% in 2005 and those of below 1.0 or 2.0 levels increased from 17.9% (2001) to 27.7% (2005). The protective levels of ND antibody titer with higher 5.0 levels increased from 2.1% in 2001 to 8.8% in 2005.

• 한국동물위생학회지
• /
• 제29권3호
• /
• pp.365-376
• /
• 2006

To identify immune response of leukocytes in peripheral blood of cattle vaccinated with an attenuated live Akabane virus vaccine, leukocytes were reacted with monoclonal antibodies which are specific to bovine lymphocyte surface antigens and assayed by the flow cytometry. Serum neutralizing (SN) test was used to measure antibody titers after vaccination, SN antibody was appeared to 7 days post-vaccination (PV) and 2-8 antibody titers were observed in 14 days PV. Proportion of $CD8^-$ MHC $class II^+$ expressing cells were rapidly increased at 3 days PV. $CD8^+$ MHC $class II^-$ cells were increased at 7 days PV. $CD4^+CD8^-,\;WC^+CD4^-,\;CD4^+CD8^+,\;WC1^-CD4^+, \;WC1^-CD8^+$, and $CD4^-CD8^+$ cells were highly increased at 3, 3, 7, 7, 14, 14 days PV, respectively.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권5호
• /
• pp.671-676
• /
• 2008

Forty-eight Naemi lambs (avg. BW 31.7 kg) were transported by truck for a distance of 1,450 km from Al-Jouf to Riyadh, Saudi Arabia. On arrival day, the lambs were randomly allocated to four groups receiving diets supplemented with 0.0, 0.3, 0.6 and 0.9 ppm organic chromium (Cr). Each group consisted of four separately housed replicates of three lambs each. The animals were fed ad libitum on a grower diet for 84 days. Blood samples were obtained shortly before transportation, upon arrival and at weekly intervals thereafter from all lambs for analysis of plasma and serum. Plasma glucose and serum cortisol, total protein, albumin, urea-N and total cholesterol concentrations were determined. A cursory clinical examination of the lambs, along with rectal temperature, was undertaken at different intervals during the experiment. The lambs were inoculated each with 2 ml i.v. chicken red blood cells (CRBC) on days 0, 21, and 42. Serum total, IgG and IgM antibody titers were determined at weekly intervals post-immunization. An in vivo intradermal hypersensitivity test was carried out on 6 lambs from each group on days 10 and 70. Transportation of the lambs resulted in a significant (p<0.001) elevation of serum cortisol, total protein and albumin levels, as well as increased plasma glucose concentration, with corresponding decrease in total cholesterol, while blood urea-N remained largely unchanged. These constituents returned to normal levels during subsequent weeks, with no significant differences in their concentrations being observed between the Cr-supplemented groups and controls. Rise in rectal temperature after transportation was reduced to a greater extent (p<0.05) in Cr-supplemented versus control lambs. Total, IgG and IgM antibody titers against CRBC rose significantly (p<0.05) during immunizations in all groups, with significantly and linearly higher (p<0.05) total and IgG titers in Cr-supplemented versus control lambs. By contrast, no significant effect due to Cr supplementation was recorded in IgG titers, which increased equally in Cr-fed and control groups. Skin thickness in response to intradermal inoculation of phytohaemagglutinin (PHA) was also significantly (p<0.01) increased as a result of Cr supplementation. These results indicate that dietary Cr supplementation might be useful during stress especially for enhancing immune responses in transport-stressed lambs.

• 한국동물위생학회지
• /
• 제18권3호
• /
• pp.36-41
• /
• 1995

This study was conducted to determine the serum antibodies against toxoplasma in bovine from gyeongnam district by latex agglutination(LA) test. LA test was carried out with Toxo-MT kit(Eikon chemical co). The result obtained were summerized as follow： 1. Positive rates of toxoplasma antibodies in 1,488 bovine sera was 5.0%(75 cases) by LA test. 2. The toxoplasma antibody detection rates against 823 korean cattle and 865 dairy cattle were 1.8%(11 cases) and 7.4%(64 cases) respectively. 3. In LA test serum antibody titers in 75 positive sera were shown as 54 cases(72.0% in 1：32, 9(12.0%) in 1：64, 7(9.4%) in 1：128, 3(3.40%) in 1：256, 1(1.3%) in 512 and 1(1.3%) in 1：2,048 respectively. 4. Positive rates of toxoplasma antibodies in cattls sera from each area were 0.2∼22.0%.

• Journal of Periodontal and Implant Science
• /
• 제30권3호
• /
• pp.687-700
• /
• 2000

Antigen-specific T cell clones were obtained from mice immunized with Fusobacterium nucleatum ATCC 10953(F .nucleatum) and/or Porphyromonas gingi valis 381(P. gingivalis). 10 Balb/c mice per group were immunized with F. nucleatum followed by P. gingivalis, or with P. gingivalis alone by intraperitoneal injection of viable microorganisms. Spleen T cells were isolated and stimulated in vitro with viable P. gingivalis cells to establish P. gingivalisspecific T cell clones. T cell phenotypes and cytokine profiles were determined along with T cell responsiveness to F .nucleatum or P. gingivalis. Serum IgG antibody titers to F. nucleatum or P. gingivalis were also determined by ELISA. All the T cell clones derived from mice immunized with F. nucleatum followed by P. gingivalis demonstrated Th2 subsets, while those from mice immunized with P. gingivalis alone demonstrated Th1 subsets based on the flow cytometric analysis and cytokine profiles, All T cells clones from both groups were cross-reactive to both P. gingivalis and F. nucleatum antigens. Phenotypes of T cell clones were all positive for CD4. Mean post-immune serum IgG antibody levels to F. nucleatum or P . gingivalis were significantly higher than the pre-immune levels(p <0.01, respectively). There were no significant differences in the antibody titers between the two groups. It was concluded that P. gingivalis-specific T cells initially primed by cross-reactive F. nucleatum antigens were polarized to Th2 subsets, while T cells stimulated with P. gingivalis alone maintained the profile of Th1 subset.

• 한국임상수의학회지
• /
• 제11권2호
• /
• pp.585-592
• /
• 1994

A consecutive Jiao Chio acupuncture therapy was performed for 3 days in the 45-90 days old 11 calves of which have been shown severe watery diarrhea. The discharge of the infected calves was yellowish brown in color. Two calves of these patients were infected wi pulmonary disease as well as diarrhea. Thus, Su Qi and Fei Yu acupuncture therapy was carried out additionally after dosing with antibiotics twice for The two infected calves. Blood chemical values and serum neutralizing antibody titers were checked, and total blood cell count was also carried out to know the therapeutic effect before and after(21 days) acupuncture therapeutics. The results are as follows ; 1, The diarrhea has ceased one day after begining of the acupuncture therapy in 5 calves, and the cessation of the diarrhea in remaining calves occurred in 1 calf each on 3rd and 4th day, and 2 calves on 6th day, respectively. Two calves infected with pulmonary disease as well diarrhea were cured 8 days after the begining of the therapcutics. 2. Rotaviruses wire detected in the feces of 2 calves, and bovine diarrhea viruses were detected in the 8 calves by the test for serum neutralizing antibody titers, and bovine coronaviruses were also detected in 5 calves. Four calves of the 5 bovine coronavirus infected calves were also infected with bovine diarrhea viruses. 3. Total leucocyte number, total amount of serum protein, and amount of fibringen were slightly increased, while total erythrocyte number, and erythrocyte packed cell volume were slightly decreased. These valucs were statistically not significant. Electrolytes of Na/sup +/, K/sup +/ and Cl/sup -/ were slightly decreased but these values also were not significant. These results indicate that the acupuncture therapeutics arc significantly effective to remove the viral diarrhea in the young calves.

• 한국동물위생학회지
• /
• 제42권4호
• /
• pp.177-181
• /
• 2019

Q fever is a zoonosis caused by Coxiella burnetti that is important not only for livestock but also for humans. This research aims to investigate infection rate of Q fever by examining the prevalence of antibody titers in cattle in Sejong City. From March to October 2018, 505 samples of Hanwoo serum and 47 samples of bulk-tank milk were collected and analyzed using ELISA. According to the result, 25 (53.2%) out of 47 bulk-tank milk samples were antibody positive and 28 (5.5%) out of 505 cows were antibody positive. The seroprevalence of C. burnetii increased with age (<1 y=0%, 1~2 y=4.1%, 3~5 y=7.5%, ≥6 y=9.6%) and was higher in females (6.5%) compared to males (0%).

• 대한수의학회지
• /
• 제56권2호
• /
• pp.121-123
• /
• 2016

A 7-year-old castrated male domestic short-hair cat presented with anorexia, constipation, depression, and voice alteration. Physical and neurological examinations revealed hyperthermia ($40.5^{\circ}C$), ventroflexion of the neck, reduced responses to external stimuli, generalized muscle weakness, and exercise intolerance. Thoracic radiographs revealed the presence of a cranial mediastinal mass. The history, clinical signs, and other examination results were compatible with acquired myasthenia gravis (MG). Acetylcholine receptor (AChR) antibody titers were determined to confirm MG and the serum AChR antibody concentration was 1.24 nmol/L (reference interval, < 0.3 nmol/L). This is the first diagnosis of acquired MG in a cat in Korea.

• 한국동물위생학회지
• /
• 제14권2호
• /
• pp.148-153
• /
• 1991

To investigate epidemological sitution of bovine viral diarrhea infection, serological survey in cattle being raised in Young Dong province were conducted. Bovine sera collected ramdomly from August 1990 to December 1990 were tested for bovine viral diarrhea virus serum neutralizing antibody titers. The results were as follows 1. BVDV SN antibody levels were considerably varies and positive rate was 58(108 heads out of 186) 2. BVDV SN antibodies to breeds of cattle was various and positive rates showed that diary cattle, beef, native cattle(Korean) were 67.52%, 59.38%, 27.00％ respectively followed in that order. 3. In the regional prevalence of BVD SN antibodies in cattle, Alpine(92％) was the highest, Young Dong south(59%) middle(44%), and North 30% followed in that order 4. In the age relatated prevalence of BVD SN antibodies, the younger than 6 month old group was the highest 65.7%, and older than 25 month old group was also at 62.2％. Then, 7 to 12 moth old group and 13 to 24 month old group showed to 58.5%, 52.1％ respectively. 5. The geometric mean titer (log2) of 108 cattle serum samples showing positive BVD SN antibodies was 4.3. 6. In the geometric mean titer(log2) according to age, younger than 6 month old group (5.2) was the highest, then 7 to 12 month old group 2.8(SD=1.94 standard deviation) was lowliest.