• Journal of applied mathematics & informatics
• /
• 제12권1_2호
• /
• pp.155-164
• /
• 2003

A method is proposed to predict the distances between given residue pairs (between C$\sub$${\alpha}$/ atoms) of a protein using a sequence to structure mapping by indefinite quadratic approximation. The prediction technique requires a data fitting in three dimensional space with coordinates of the residues of known structured proteins and leads to a numerical ref resentation of 20 amino acids by minimizing a large least norm iteratively. These approximations are used in distance prediction for given residue pairs. Some computational experience on a test set of small proteins from Brookhaven Protein Data Bank are given.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제20권2호
• /
• pp.37-43
• /
• 2010

The wild silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae) is a polyphagous silk producing insect that feeds on Terminalia arjuna, T. tomentosa and Shorea robusta and is distributed in the forest belts in different states of India. Phenotypically distinct populations of the A. mylitta are called "eco-race" or "ecotypes". Genetic improvement of this wild silkworm has not progressed much due to lack of adequate information on the factors that control the expression of most of the economically important traits. Considering the amazing technological advances taking place in molecular biology, it is envisaged that it is now possible to take greater control on these intractable traits if a combination of genetic, molecular and bioinformatics tools are used. Linkage disequilibrium (LD) mapping is one such approach that has extensively been used in both animal and plant system to identify quantitative trait loci (QTLs) for a number of economically important traits. LD mapping has a number of advantages over conventional biparental linkage mapping. Therefore, LD mapping is considered more efficient for gene discovery to meet the challenge of connecting sequence diversity with heritable phenotypic differences. However, care must be taken to avoid detection of spurious associations which may occur due to population structure and variety interrelationships. In this review, we discuss how LD mapping is suitable for the dissection of complex traits in wild silkworms (Antheraea mylitta).

• 정보처리학회논문지B
• /
• 제17B권3호
• /
• pp.183-188
• /
• 2010

본 연구에서는 여러 이미지를 이용하여 사실적인 3차원 장면의 모델을 얻는 방법이 구현되었다. 이미지는 파라메터를 모르는 카메라를 이용하여 여러 위치에서 획득한 것을 사용하였다. 먼저 특징점 추출 및 추적 방법을 사용하여 모든 이미지에 대한 대응점들을 구하고 이 점들을 사용하여 사영복원을 구한다. 그 다음 사영 복원된 값에 여러 제약조건을 사용하여 유클리디언 복원을 하면 특징점들의 3차원 좌표값이 계산된다. 이 좌표값을 이용하여 삼각형 메쉬를 구한 후 이 면에 텍스처 맵핑을 하면 사실적인 복원이 완성된다. 전체 시스템은 C++언어로 구현하였으며, 사용자 인터페이스는 Qt 라이브러리로, 텍스처 맵핑과 모델 가시화 부분은 OpenGL 그래픽스 라이브러리로 구현하였다. 구현된 시스템의 효용성을 보이기 위해 모의 데이터와 실제 이미지 데이터를 이용하여 실험한 결과를 포함하였으며 만족할 만한 복원 결과를 얻을 수 있었다.

• 한국통신학회논문지
• /
• 제40권6호
• /
• pp.1005-1013
• /
• 2015

본 논문에서는 밀리미터파를 활용한 이동무선백홀에서 상향링크의 파일럿 신호 구조를 제안하였다. Zadoff-Chu 시퀀스를 이용하여 생성된 파일럿 신호는 두 개의 안테나 포트에 대한 송신 다이버시티를 위하여 주파수 축 상에서 교차 매핑 또는 연속 매핑되는 구조가 가능하며, 파일럿 신호의 매핑 방법에 따라 채널 추정 알고리즘이 달라진다. 시뮬레이션을 통해 지하철 환경을 가정한 레일리 페이딩 채널에서 성능을 분석한 결과, 교차 매핑 방법이 연속 매핑 방법에 비해 채널 추정의 성능 열화가 없었고 이 방법을 사용하여 상향링크 채널 추정기의 구현 복잡 도를 줄일 수 있었다.

• The Plant Pathology Journal
• /
• 제18권4호
• /
• pp.187-191
• /
• 2002

This paper describes the cloning and sequence analysis of the 5'-terminal region and full-length cDNA production of genomic RNA of Lily symptomless virus (LSV), a Species Of the genus Carlavirus. A sing1e DNA band about 600 bp harboring the 5'-end of genomic RNA of the virus was successfully amplified by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), and was cloned for nucleotide sequence determination. Sequence analysis of selected RACE cDNA clones revealed that the LSV 5'non-translated region consists of 67 nucleotides long of AT rich stretch followed GC rich from the 5'-end. To produce full-length cDNA products for the viral genomic RNA, a set of LSV-specific primers could be designed based on the obtained sequence in this study and the known sequences of 3'-terminal region for the virus. Full-length cDNA copies of LSV, an 8.4 kb long, were directly amplified by the long-template RT-PCR technique from the purified viral genomic RNA samples. This full-length cDNA copies were analyzed by restriction mapping. The molecules produced in this study can be useful for the production of in vitro infectious cDNA clone, as well as, for the completion of genomic RNA sequence and genome structure for the virus.

• 대한수학회논문집
• /
• 제11권3호
• /
• pp.725-742
• /
• 1996

In this paper we give some sharp expressions of the weakly convergent sequence coefficient WCS(X) of a Banach space X. They are used to prove fixed point theorems for involution mappings T from a weakly compact convex subset C of a Banach space X with WCS(X) > 1 into itself which $T^2$ are both of asymptotically nonexpansive type and weakly asymptotically regular on C. We also show that if X satisfies the semi-Opial property, then every nonexpansive mapping $T : C \to C$ has a fixed point. Further, some questions for asymtotically nonexpansive mappings are raised.

• 한국컴퓨터그래픽스학회논문지
• /
• 제5권2호
• /
• pp.43-52
• /
• 1999

본 논문에서는 캐릭터의 운동 표현에 필요한 데이터 구조를 시각적으로 기술할 수 있는 사용자 인터페이스에서 모션 매핑 기술을 이용하여 3차원 캐릭터의 애니메이션을 쉽게 생성할 수 있는 도구 개발에 대해 기술한다. 본 논문에서의 모션 매핑이란 한 번 생성된 애니메이션을 그대로 다른 캐릭터에 적용하여 같은 모션을 생성시키는 것으로 정의한다. 본 애니메이션 도구는 3차원 캐릭터의 기하 데이터를 이용하여 애니메이션의 생성과 변형을 대화적 방법으로 쉽게 생성시킬 수 있다. 이것은 본 연구팀에서 앞서 개발한 캐릭터 개발도구에서 생성된 3차원 캐릭터 데이터를 위의 인터페이스에서 모션 생성의 구조를 대화적으로 변형시키면서 애니메이션을 생성하도록 구성한 것이다. 기존의 다른 애니메이션 도구와 구별되는 기능으로는 캐릭터의 모델링 데이터와 모션 데이터를 분리하여 모델링 데이터가 없는 상황에서도 독립적으로 애니메이션만을 실행시킬 수 있으며 한 번 생성된 애니메이션을 모션 매핑으로 다른 캐릭터에 그대로 적용시킬 수 있다는 것이다.

• Journal of Microbiology and Biotechnology
• /
• 제3권3호
• /
• pp.146-155
• /
• 1993

A DNA fragment from an alkali-tolerent Bacillus sp., conferring strong promoter activity, was subcloned into the promoter probe plasmid pPL703 and the nucleotide sequence of this promoter region was determined. The sequence analysis suggested that this highly efficient promoter region containing the complex clustered promoters comprised three kinds of promoters (P1, P2 and P3), which are transcribed by $\sigma^B (formerly \sigma^{37}), \sigma^E(formerly \sigma^{29}) and \sigma^A (formerly \sigma^{43})$ RNA polymerase holoenzymes which play major rules at the onset of endospore formation, during sporulation and at the vegetative phase of growth, respectively. S1 nuclease mapping experiments showed that all three promoters had staggered transcription initiation points. The results of chloramphenicol acetyltransferase assay after the subcloning experiments also indicated that the expression of these clustered promoters was correlated with the programs of growth and endospore development. Promoter P1, P2 and P3 were preceded by 75% AT, 79% AT and 81% AT regions, respectively, and a partial deletion of AT-rich region prevented transcription from promoter P1 in vivo. Two sets of 5 -AGTGTT-3 sequences and inverted repeat sequences located around the promoter P1 were speculated as the possible cis acting sites for the catabolite repression in B. subtilis. In vivo transcripts from these sequence regions may be able to form a secondary structure, however, the possibility that a regulatory protein induced by the excess amount of glucose could be bound to such a domain for crucial action remains to be determined.

• Journal of Ginseng Research
• /
• 제19권1호
• /
• pp.56-61
• /
• 1995

Molecular cloning and restriction mapping on ATPase $\alpha$-subunit gene (atpA) were carried out to obtain genomic information concerned with the gene structure and organization in Korean ginseng mitochondria. Two different clones containing the homologous sequence of atpA gene were selected from SalI and PstI libraries of mitochondrial DNA (mtDNA) of Korean ginseng. The sizes of mtDNA fragments inserted in SalI and PstI clones were 3.4 kb and 13 kb, respectively. Southern blot analysis with [$^{32}P$] labelled Oenothera atPA gene probe showed that atpA gene sequence was located in 2.0 kb XkaI fragment in PstI clone and in 1.7 kb XbaI fragment in SalI clone. A partial sequening ascertained that the SalI clone included about 1.2 kb fragment from SalI restriction site to C-terminal sequence of this gene but about 0.3 kb N-terminal sequence of open reading frame was abscent. The PstI fragment was enough large to cover the full sequence of atpA gene. The same restriction pattern of the overlapped region suggests that both clones include the same fragment of atiA locus. Data of Southern blot analysis and partial nucleotide sequencing suggested that mtDNA of Korean ginseng has a single copy of atpA gene. Key words ATPase a-subunit, mitochondrial DNA, Panax ginseng.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제10권2호
• /
• pp.79-86
• /
• 2005

Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.