• Title/Summary/Keyword: sequence to structure mapping

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A QUADRATIC APPROXIMATION FOR PROTEIN SEQUENCE TO STRUCTURE MAPPING

  • Oh, Se-Young;Yun, Jae-Heon;Chung, Sei-Young
    • Journal of applied mathematics & informatics
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    • v.12 no.1_2
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    • pp.155-164
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    • 2003
  • A method is proposed to predict the distances between given residue pairs (between C$\sub$${\alpha}$/ atoms) of a protein using a sequence to structure mapping by indefinite quadratic approximation. The prediction technique requires a data fitting in three dimensional space with coordinates of the residues of known structured proteins and leads to a numerical ref resentation of 20 amino acids by minimizing a large least norm iteratively. These approximations are used in distance prediction for given residue pairs. Some computational experience on a test set of small proteins from Brookhaven Protein Data Bank are given.

Prospects of Application of Linkage Disequilibrium Mapping for Crop Improvement in Wild Silkworm (Antheraea mylitta Drury)

  • Vijayan, Kunjupillai;Singh, Ravindra Nath;Saratchandra, Beera
    • International Journal of Industrial Entomology and Biomaterials
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    • v.20 no.2
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    • pp.37-43
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    • 2010
  • The wild silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae) is a polyphagous silk producing insect that feeds on Terminalia arjuna, T. tomentosa and Shorea robusta and is distributed in the forest belts in different states of India. Phenotypically distinct populations of the A. mylitta are called "eco-race" or "ecotypes". Genetic improvement of this wild silkworm has not progressed much due to lack of adequate information on the factors that control the expression of most of the economically important traits. Considering the amazing technological advances taking place in molecular biology, it is envisaged that it is now possible to take greater control on these intractable traits if a combination of genetic, molecular and bioinformatics tools are used. Linkage disequilibrium (LD) mapping is one such approach that has extensively been used in both animal and plant system to identify quantitative trait loci (QTLs) for a number of economically important traits. LD mapping has a number of advantages over conventional biparental linkage mapping. Therefore, LD mapping is considered more efficient for gene discovery to meet the challenge of connecting sequence diversity with heritable phenotypic differences. However, care must be taken to avoid detection of spurious associations which may occur due to population structure and variety interrelationships. In this review, we discuss how LD mapping is suitable for the dissection of complex traits in wild silkworms (Antheraea mylitta).

Realistic 3D Scene Reconstruction from an Image Sequence (연속적인 이미지를 이용한 3차원 장면의 사실적인 복원)

  • Jun, Hee-Sung
    • The KIPS Transactions:PartB
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    • v.17B no.3
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    • pp.183-188
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    • 2010
  • A factorization-based 3D reconstruction system is realized to recover 3D scene from an image sequence. The image sequence is captured from uncalibrated perspective camera from several views. Many matched feature points over all images are obtained by feature tracking method. Then, these data are supplied to the 3D reconstruction module to obtain the projective reconstruction. Projective reconstruction is converted to Euclidean reconstruction by enforcing several metric constraints. After many triangular meshes are obtained, realistic reconstruction of 3D models are finished by texture mapping. The developed system is implemented in C++, and Qt library is used to implement the system user interface. OpenGL graphics library is used to realize the texture mapping routine and the model visualization program. Experimental results using synthetic and real image data are included to demonstrate the effectiveness of the developed system.

Uplink Pilot Signal Design for Mobile Wireless Backhaul (이동무선백홀을 위한 상향링크 파일럿 신호 설계)

  • Choi, Seung Nam;Kim, Ilgyu;Kim, Dae Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.40 no.6
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    • pp.1005-1013
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    • 2015
  • In this paper, an uplink pilot signal structure is proposed for millimeter wave(mmWave)-based mobile wireless backhaul. For the transmit diversity of two antenna ports, uplink pilot signals generated from the Zadoff-Chu sequence can be mapped in an interleaved mode or continuous mode on the frequency axis, and channel estimation algorithms are different depending on the pilot signal mapping schemes. Through a simulation under Rayleigh fading channel assuming a subway scenario, the interleaved mapping scheme showed no performance degradation compared to the continuous mapping scheme and the implementation complexity of the uplink channel estimator was reduced due to the interleaved mapping scheme.

Cloning of the 5'-end and Amplification of Full-Length cDNA of Genomic RNA of Lily symptomless virus

  • Park, Seon-Ah;Ryu, Ki-Hyun
    • The Plant Pathology Journal
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    • v.18 no.4
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    • pp.187-191
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    • 2002
  • This paper describes the cloning and sequence analysis of the 5'-terminal region and full-length cDNA production of genomic RNA of Lily symptomless virus (LSV), a Species Of the genus Carlavirus. A sing1e DNA band about 600 bp harboring the 5'-end of genomic RNA of the virus was successfully amplified by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), and was cloned for nucleotide sequence determination. Sequence analysis of selected RACE cDNA clones revealed that the LSV 5'non-translated region consists of 67 nucleotides long of AT rich stretch followed GC rich from the 5'-end. To produce full-length cDNA products for the viral genomic RNA, a set of LSV-specific primers could be designed based on the obtained sequence in this study and the known sequences of 3'-terminal region for the virus. Full-length cDNA copies of LSV, an 8.4 kb long, were directly amplified by the long-template RT-PCR technique from the purified viral genomic RNA samples. This full-length cDNA copies were analyzed by restriction mapping. The molecules produced in this study can be useful for the production of in vitro infectious cDNA clone, as well as, for the completion of genomic RNA sequence and genome structure for the virus.

Some results on metric fixed point theory and open problems

  • Kim, Tae-Hwa;Park, Kyung-Mee
    • Communications of the Korean Mathematical Society
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    • v.11 no.3
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    • pp.725-742
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    • 1996
  • In this paper we give some sharp expressions of the weakly convergent sequence coefficient WCS(X) of a Banach space X. They are used to prove fixed point theorems for involution mappings T from a weakly compact convex subset C of a Banach space X with WCS(X) > 1 into itself which $T^2$ are both of asymptotically nonexpansive type and weakly asymptotically regular on C. We also show that if X satisfies the semi-Opial property, then every nonexpansive mapping $T : C \to C$ has a fixed point. Further, some questions for asymtotically nonexpansive mappings are raised.

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A Character Animation Tool Based on Motion Mapping (모션 매핑 기반의 캐릭터 애니메이션 개발 도구)

  • Lee, Minguen;Lee, Myeong Won
    • Journal of the Korea Computer Graphics Society
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    • v.5 no.2
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    • pp.43-52
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    • 1999
  • In this paper, we present an animation toolkit based on motion mapping technique in a graphics user interface that can represent data structures necessary for generating character motions. The motion mapping means that an animation sequence generated once can be mapped to another object directly according a data structure in the graphics user interface. Users can generate animation sequences interactively using a mouse. These are obtained automatically by modifying motion data structures interactively. Compared with other conventional tools, the toolkit has different features that two hierarchical structures necessary for representing modeling and animation data are managed independently each other, and that animations generated can be applied to any other characters by connecting the two hierarchical structures in the user interface.

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Structure and Regulation of a Complex Promoter Region from an Alkali-tolerent Bacillus sp.

  • Kim, Jin-Man;Park, Hee-Kyung;Park, Young-Seo;Yum, Do-Young;Bai, Dong-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.3 no.3
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    • pp.146-155
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    • 1993
  • A DNA fragment from an alkali-tolerent Bacillus sp., conferring strong promoter activity, was subcloned into the promoter probe plasmid pPL703 and the nucleotide sequence of this promoter region was determined. The sequence analysis suggested that this highly efficient promoter region containing the complex clustered promoters comprised three kinds of promoters (P1, P2 and P3), which are transcribed by $\sigma^B (formerly \sigma^{37}), \sigma^E(formerly \sigma^{29}) and \sigma^A (formerly \sigma^{43})$ RNA polymerase holoenzymes which play major rules at the onset of endospore formation, during sporulation and at the vegetative phase of growth, respectively. S1 nuclease mapping experiments showed that all three promoters had staggered transcription initiation points. The results of chloramphenicol acetyltransferase assay after the subcloning experiments also indicated that the expression of these clustered promoters was correlated with the programs of growth and endospore development. Promoter P1, P2 and P3 were preceded by 75% AT, 79% AT and 81% AT regions, respectively, and a partial deletion of AT-rich region prevented transcription from promoter P1 in vivo. Two sets of 5 -AGTGTT-3 sequences and inverted repeat sequences located around the promoter P1 were speculated as the possible cis acting sites for the catabolite repression in B. subtilis. In vivo transcripts from these sequence regions may be able to form a secondary structure, however, the possibility that a regulatory protein induced by the excess amount of glucose could be bound to such a domain for crucial action remains to be determined.

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Molecular Cloning of ATPase $\alpha$-Subunit Gene from Mitochondria of Korean Ginseng (Panu ginseng C.A. Meyer) (고려인삼(Panax ginseng C.A. Meyer) ATPase $\alpha$-subunit 유전자의 Cloning)

  • Park, Ui-Sun;Choi, Kwan-Sam;Kim, Kab-Sig;Kim, Nam-Won;Choi, Kwang-Tae
    • Journal of Ginseng Research
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    • v.19 no.1
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    • pp.56-61
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    • 1995
  • Molecular cloning and restriction mapping on ATPase $\alpha$-subunit gene (atpA) were carried out to obtain genomic information concerned with the gene structure and organization in Korean ginseng mitochondria. Two different clones containing the homologous sequence of atpA gene were selected from SalI and PstI libraries of mitochondrial DNA (mtDNA) of Korean ginseng. The sizes of mtDNA fragments inserted in SalI and PstI clones were 3.4 kb and 13 kb, respectively. Southern blot analysis with [$^{32}P$] labelled Oenothera atPA gene probe showed that atpA gene sequence was located in 2.0 kb XkaI fragment in PstI clone and in 1.7 kb XbaI fragment in SalI clone. A partial sequening ascertained that the SalI clone included about 1.2 kb fragment from SalI restriction site to C-terminal sequence of this gene but about 0.3 kb N-terminal sequence of open reading frame was abscent. The PstI fragment was enough large to cover the full sequence of atpA gene. The same restriction pattern of the overlapped region suggests that both clones include the same fragment of atiA locus. Data of Southern blot analysis and partial nucleotide sequencing suggested that mtDNA of Korean ginseng has a single copy of atpA gene. Key words ATPase a-subunit, mitochondrial DNA, Panax ginseng.

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Inter Simple Sequence Repeat (ISSR) Polymorphism and Its Application in Mulberry Genome Analysis

  • Vijayan Kunjupillai
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.2
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    • pp.79-86
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    • 2005
  • Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.