• Journal of Plant Biotechnology
• /
• v.46 no.2
• /
• pp.79-87
• /
• 2019

One of wild diploid Solanum species, Solanum chacoense, is one of the excellent resources for potato breeding because it is resistant to several important pathogens, but the species is not sexually compatible with potato (S. tuberosum) causing the limitation of sexual hybridization between S. tuberosum and S. chacoense. Therefore, diverse traits regarding resistance from the species can be introgressed into potato via somatic hybridization. After cell fusion, the identification of fusion products is crucial with molecular markers. In this study, S. chacoense specific markers were developed by comparing the chloroplast genome (cpDNA) sequence of S. chacoense obtained by NGS (next-generation sequencing) technology with those of five other Solanum species. A full length of the cpDNA sequence is 155,532 bp and its structure is similar to other Solanum species. Phylogenetic analysis resulted that S. chacoense is most closely located with S. commersonii. Sequence alignment with cpDNA sequences of six other Solanum species identified two InDels and 37 SNPs specific sequences in S. chacoense. Based on these InDels and SNPs regions, four markers for distingushing S. chacoense from other Solanum species were developed. These results obtained in this research could help breeders select breeding lines and facilitate breeding using S. chacoense in potato breeding.

• Steel and Composite Structures
• /
• v.29 no.6
• /
• pp.749-758
• /
• 2018

This article derived a hybrid coupling technique using the higher-order displacement polynomial and three soft computing techniques (teaching learning-based optimization, particle swarm optimization, and artificial bee colony) to predict the optimal stacking sequence of the layered structure and the corresponding frequency values. The higher-order displacement kinematics is adopted for the mathematical model derivation considering the necessary stress and stain continuity and the elimination of shear correction factor. A nine noded isoparametric Lagrangian element (eighty-one degrees of freedom at each node) is engaged for the discretisation and the desired model equation derived via the classical Hamilton's principle. Subsequently, three soft computing techniques are employed to predict the maximum natural frequency values corresponding to their optimum layer sequences via a suitable home-made computer code. The finite element convergence rate including the optimal solution stability is established through the iterative solutions. Further, the predicted optimal stacking sequence including the accuracy of the frequency values are verified with adequate comparison studies. Lastly, the derived hybrid models are explored further to by solving different numerical examples for the combined structural parameters (length to width ratio, length to thickness ratio and orthotropicity on frequency and layer-sequence) and the implicit behavior discuss in details.

• Journal of Veterinary Science
• /
• v.22 no.6
• /
• pp.66.1-66.9
• /
• 2021

Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%-86.8% and 67.7%-75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.

• Journal of the Korean Society of Industry Convergence
• /
• v.26 no.1
• /
• pp.201-209
• /
• 2023

Recently, fiber-reinforced composites have been widely used in various industrials fields. In this study, the mechanical behavior, especially fracture behavior, of biomimetic fiber-reinforced composites subjected to pressure loading was analyzed using finite element analysis (FEA). The fiber alignments in the biomimetic composites formed a helicoidal structure, wherein a stacking sequence involved a gradual rotation of each ply in the multi-layered laminated composites. For comparison, cross-ply composite samples with fibers arranged at 0° and 90° were prepared and analyzed. In addition, the mechanical behavior was analyzed based on combinations of the stacking sequence of carbon-fiber composites and glass-fiber composites. The FEA results showed that, when compared with the cross-ply samples, the mechanical properties of the biomimetic composites were considerably improved under pressure loading, which was applied to one side of the composites. Thus, the biomimetic helicoidal structure significantly improved the mechanical properties of the composites. Placing materials having high elasticity and strength in the outermost layers (the layer of the side on which pressure was applied and the opposite side layer) of the composites also significantly contributed to improving the mechanical properties of the composites.

• 제어로봇시스템학회:학술대회논문집
• /
• 1997.10a
• /
• pp.597-600
• /
• 1997

In this paper, the authors propose a new method for linearizing a nonlinear dynamical system by use of polynomial compensation. In this method, an M-sequence is applied to the nonlinear system and the crosscorrelation function between the input and the output gives us every crosssections of Volterra kernels of the nonlinear system up to 3rd order. We construct a polynomial compensation function from comparison between lst order Volterra kernel and high order kernels. The polynomial compensation function is, in this case, of third order whose coefficients are variable depending on the amplitude of the input signal. Once we can get compensation function of nonlinear system, we can construct a linearization scheme of the nonlinear system. That is. the effect of second and third order Volterra kernels are subtracted from the output, thus we obtain a sort of linearized output. The authors applied this method to a saturation-type nonlinear system by simulation, and the results show good agreement with the theoretical considerations.

• Journal of Forest and Environmental Science
• /
• v.24 no.2
• /
• pp.61-68
• /
• 2008

Acrylamide is present as a contaminant in heated food products, predominantly from the precursor asparagine. Nonanchored inter simple sequence repeats (ISSRs) are arbitrary multiloci markers produced by PCR amplification with a microsatellite primer. In order to assess the feasibility of microsatellite primers as markers for DNA damage, the study was conducted on common bean (Phaseolus vulgaris L.) exposed to different concentrations of acrylamide. Polymorphisms were abundant among plant samples treated with acrylamide in comparison to control (untreated one) tested with 4- tri-nucleotide, 2 tetra-nucleotide, and 3- dinucelotide primers. The primer (CCG)4 was the best tested primer to generate polymorphism between the DNA of plants treated or not by acrylamide. Polymorphisms became evident as the presence and absence of DNA fragments in treated samples compared with the untreated one. The highest number of DNA variation on ISSR patterns was observed at the micromollar concentrations of acrylamide. Acrylamide was able to induce DNA damage in non concentration-dependent manner with effectiveness at micromollar concentrations. This study demonstrated that ISSR markers can be highly reliable for identification of DNA damage induced by acrylamide.

• Journal of The Korean Astronomical Society
• /
• v.34 no.3
• /
• pp.143-147
• /
• 2001

UBVI CCD photometry of open cluster NGC 2324 is presented. C-M diagrams of this cluster show well-defined main sequence with a red giant clump centered at B - V =1.05, V =13.45. We derived the major cluster characteristics; E(B - V)=0.17$\pm$0.12 from color-color diagram and mean color of red giant clump stars, (m - M)o=13.1$\pm$0.1 from zero age main sequence fitting, and [Fe/H]$\~$-0.32 from comparison the theoretical model developed by Bertelli et al. (1994) to the observed C-M diagrams. We estimate the age of NGC 2324 to be log t$\~$8.8 by applying isochrone fitting and morphological age index method.

• Animal cells and systems
• /
• v.13 no.3
• /
• pp.345-350
• /
• 2009

We have isolated and characterized a new insect chicken type (c-type) lysozyme gene from the common cutworm, Spodoptera litura. The full-length cDNA of Spodoptera lysozyme is cloned by rapid amplification of cDNA ends PCR (RACE-PCR). The isolated cDNA consists of 1039 bp including the coding region for a 142-amino acid residue polypeptide, which included a signal peptide of 21-amino acid residue and a mature protein of 121-amino acid residue. The predicted molecular weight of mature lysozyme and its theoretical isoelectric point from amino acid composition is 13964.8 Da and 9.05, respectively. The deduced amino acid sequence of Spodoptera lysozyme gene shows the highest similarity (96.7%) to Spodoptera exigua lysozyme among other lepidopteran species. Amino acid sequence comparison with other the c-type lysozymes, Spodoptera lysozyme has the completely conserved $Glu^{32}$ and $Asp^{50}$ of the active site and eight Cys residues are completely conserved in the same position as that of other lepidopteran lysozymes.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.125.1-125
• /
• 2003

Pepper anthracnose is one of the major limiting factors in pepper production. Boring last over 10 years, Colletotrichum gloeosporioides has been known as the most prevalent species among five Colletotrichum spp. involved as anthracnose causing agents. Recently, however, the change of major species with pepper anthracnose has been proposed. Identification study was peformed on 12 test isolates collected from anthracnose disease symptoms on pepper during 2001-2002 and 25 reference isolates obtained from several other host plants. The identification of the isolates with morphological observation and IfS region sequence comparison resulted that 11 ones from 12 test isolates colleted from pepper anthracnose during 2001-2002 were identified as C. acutatum. PCR using species-specific primers designed from ITS region sequence suggested a rapid diagnosis method in identifying C. acutatum from C. gloeosporioides.

• Journal of Korean Society for Quality Management
• /
• v.17 no.2
• /
• pp.121-141
• /
• 1989

This paper is concerned with a development and evaluation of heuristics for the multi-stage sequence dependent flowshop sequencing. Eighteen heuristics, CAM1, CAM2, and etc., are proposed. The performance measure is a makespan which is to be minimized. The experiment for each algorithm is designed for a 4*3*3 factorial design with 360 observations. The experimental factors are PS(ratio of processing times to setup times), M(number of machines), N(number of jobs). The makespan of the proposed heuristics is compared with the optimal makespan obtained by the complete enumeration of schedules. This yardstick of comparison is called a relative error. The mean relative errors of the eighteen heuristics are from 2.048% to 8.717%. The computational results are analysed using SPSS. The experimental results show that the three factors are statistically significant at 5% level. The simulation for the large size problems is conducted to show having the similar computational results like the small size problems.