• Journal of Ginseng Research
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• 제43권3호
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• pp.421-430
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• 2019

Background: The ginsenoside Rg3, one of active components of red ginseng, has chemopreventive and anticancer potential. Cancer stem cells retain self-renewal properties which account for cancer recurrence and resistance to anticancer therapy. In our present study, we investigated whether the standardized Korean Red Ginseng extract (RGE) and Rg3 could modulate the manifestation of breast cancer stem cell-like features through regulation of self-renewal activity. Methods: The effects of RGE and Rg3 on the proportion of $CD44^{high}/CD24^{low}$ cells, as representative characteristics of stem-like breast cancer cells, were determined by flow cytometry. The mammosphere formation assay was performed to assess self-renewal capacities of breast cancer cells. Aldehyde dehydrogenase activity of MCF-7 mammospheres was measured by the ALDEFLUOR assay. The expression levels of Sox-2, Bmi-1, and P-Akt and the nuclear localization of hypoxia inducible $factor-1{\alpha}$ in MCF-7 mammospheres were verified by immunoblot analysis. Results: Both RGE and Rg3 decreased the viability of breast cancer cells and significantly reduced the populations of $CD44^{high}/CD24^{low}$ in MDA-MB-231 cells. RGE and Rg3 treatment attenuated the expression of Sox-2 and Bmi-1 by inhibiting the nuclear localization of hypoxia inducible $factor-1{\alpha}$ in MCF-7 mammospheres. Suppression of the manifestation of breast cancer stem cell-like properties by Rg3 was mediated through the blockade of Akt-mediated self-renewal signaling. Conclusion: This study suggests that Rg3 has a therapeutic potential targeting breast cancer stem cells.

• BMB Reports
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• 제51권2호
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• pp.92-97
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• 2018

B cell leukemia/lymphoma 3 (Bcl3) plays a pivotal role in immune homeostasis, cellular proliferation, and cell survival, as a co-activator or co-repressor of transcription of the $NF-{\kappa}B$ family. Recently, it was reported that Bcl3 positively regulates pluripotency genes, including Oct4, in mouse embryonic stem cells (mESCs). However, the role of Bcl3 in the maintenance of pluripotency and self-renewal activity is not fully established. Here, we report the dynamic regulation of the proliferation, pluripotency, and self-renewal of mESCs by Bcl3 via an influence on Nanog transcriptional activity. Bcl3 expression is predominantly observed in immature mESCs, but significantly decreased during cell differentiation by LIF depletion and in mESC-derived EBs. Importantly, the knockdown of Bcl3 resulted in the loss of self-renewal ability and decreased cell proliferation. Similarly, the ectopic expression of Bcl3 also resulted in a significant reduction of proliferation, and the self-renewal of mESCs was demonstrated by alkaline phosphatase staining and clonogenic single cell-derived colony assay. We further examined that Bcl3-mediated regulation of Nanog transcriptional activity in mESCs, which indicated that Bcl3 acts as a transcriptional repressor of Nanog expression in mESCs. In conclusion, we demonstrated that a sufficient concentration of Bcl3 in mESCs plays a critical role in the maintenance of pluripotency and the self-renewal of mESCs via the regulation of Nanog transcriptional activity.

• 한국임상보건과학회지
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• 제8권2호
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• pp.1436-1443
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• 2020

Purpose: The purpose of this study was to differences in the expression rate of Porphyromonas gingivalis according to smoking status, smoking amount and period of smoking. Methods: At the time of investigation, 30 smokers and non-smokers were recruited among patients with periodontitis with a probing pocket depth(PPD) of 4 mm or more. General information was collected using a self-questionnaire, and the average value was used by a dentist to measure the probing pocket depth of three times each for the first or second molar. Plaque collection and analysis were performed by collecting only subgingival plaque using a conventional method, and the expression rate of P. gingivalis was confirmed using polymerase chain reaction (PCR). For statistical analysis, the SPSS Ver 25.0 program was used. Results: Smoking did not have a significant effect on the expression of P. gingivalis, but it did affect the expression of more type II genotypes (p<0.05). In addition, smokers had more slight periodontal pocket, and the amount and duration of smoking did not affect the expression of P. gingivalis. Conclusions: In the future, it is necessary to reinforce the group of smokers and non-smokers with healthy oral conditions, and to investigate the quantitative difference in the expression rate and genotype of P. gingivalis over time of harmful substances in smoking.

• 가정과삶의질연구
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• 제20권2호
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• pp.203-214
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• 2002

This study of the relationships between children's depression and anxiety and their children's self-esteem and emotional intelligence had a sample of 984 5th grade children The higher the levels of children's depression and anxiety were, the lower their self-esteem consisting of scholastic competence, social acceptance, atheletic competence, physical appearance, behavioral conduct, and global self-worth were. The higher the levels of children's depression and anxiety were, the lower their emotional intelligence(EI) consisting of self-regulation and emotion utilization, perception of emotion, other-regulation and self-expression were. For boys, scholastic competence and social competence among children's self-esteem factors were the factors best explained by children's depression and anxiety. In addition, boy's perception of emotion was explained by their depression and anxiety. For girls, on the other hand global self-worth and social competence among self-esteem factors were the two factors best explained by their depression and anxiety. Self-regulation and emotion utilization was the El factor best explained by depression and anxiety among girls.

• 패션비즈니스
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• 제2권2호
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• pp.66-76
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• 1998

The purpose of this study was to examine the proximity of clothing to self and proximity of cosmetic to self by Degree of Cosmetics. The scales used in this study include the arranged on the basis of Sontag (1978) 's study for proximity of clothing to self, and the scale developed by the researcher for proximity of cosmetic to self. The subjects of this study were 885 adult women in Taegu. The data collected were analyzed by using MANOVA, ANOVA, and the Cronbach a was also applied. The results of this study were summarized as follows: 1. The persons with high degree of cosmetics showed high degrees of joy of change, self-value expression, novelty, self-consciousness and consciousness to others in the sub-factors of proximity of clothing to self, while there was not significant difference in physical satisfaction. 2. The persons with high degree of cosmetics showed high degrees of social confidence, skin care, joy of change, self-satisfaction, instrument of disguise in the sub-factors of proximity of cosmetic to self.

• International Journal of Advanced Culture Technology
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• 제11권4호
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• pp.429-434
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• 2023

This is a single-group pre-post study conducted to determine the effect of a calligraphy program on nursing students' career decision-making self-efficacy and self- leadership. The data collected from 231 second-year nursing students were analyzed using SPSS WIN 25.0 to determine descriptive statistics, paired t-test to analyze the effect of calligraphy, and Pearson correlation coefficient to determine the relationship between career decision self-efficacy and self-leadership. After the program, both career decision self-efficacy (t=7.23, p<.001) and self-leadership (t=6.81, p<.001) improved. The analysis of career decision self-efficacy and self-leadership after the program showed a strong correlation (r=.849, p<.001). The results of this study are significant in that they validate the effectiveness of a calligraphy program in promoting self-leadership and career decision-making self-efficacy among nursing students who do not have clear career goals. Students' overall satisfaction with the calligraphy program was 4/5 points, and they most highly evaluated the smooth interaction between fellow students through the program. At a time when the importance of cultural arts education is gradually increasing in social and educational fields around the world, calligraphy has the advantage of laying the foundation for the promotion of basic arts for the revitalization of cultural arts, so educational support is needed not only in terms of improving the quality of life and pursuing self-actualization of individuals with emotional expression and creative problem-solving skills, but also at the social level.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제11권2호
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• pp.63-71
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• 2005

The purpose of this study is to find the complementary idea of the psychological elements in aesthetic expression of architectural space in the sense of healing. Marks $L\ddot{u}scher$, the swiss psychologist suggested reasonable categories of emotional character according to the couple combinations among Content, Self-Esteem, Confidence, and Liberty. His theory on healing is not only the concept but the practical method to understand and control the conflict between contrary elements of emotion. Compared to the Freud's objective theory of cause and consequence, his idea is focused on the subjective and autonomous harmony. This theory based on complementary idea is applied to psychological attitude of architects on Spatial Expression in this study. Refuge, Prospect, Flow and Void are assumed as equivalent spatial elements to the four major emotional conditions suggested by $M.L\ddot{u}scher$. The couple combinations among them also characterize the architectural characters definitely and it is expected to be the reasonable criteria in analysing aesthetic value and motive of creating human space.

• 간호행정학회지
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• 제16권4호
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• pp.428-436
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• 2010

Purpose: The purposes of this study were to describe events resulting in perceived hurts in nursing, and to identify factors influencing forgiveness in clinical nurses. Methods: The study was a descriptive correlation design. From May to July, 2009, the researcher used interviews to collect data from 148 clinical nurses from five hospitals in D city. Results: Clinical nurses received perceived hurt from peer-nurses, care-givers, and doctors, and the reasons for the perceived hurts were blame, neglect, and valuation. Levels of forgiveness in clinical nurses were different according to who caused the perceived hurt, the degree of the perceived hurt, and endeavors to resolve the perceived hurts. Levels of forgiveness in clinical nurses were negatively correlated with age, degree of perceived hurt, and degree of anger expression, and positively correlated with degree of self-esteem. The strongest predictors of forgiveness in clinical nurses were degree of anger expression, age, and degree of hurt. Conclusion: The findings of the study suggest that nursing staff should be able to identify reasons for perceived hurt in clinical nurses and provide a forgiveness program for each nursing situation.

• Molecules and Cells
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• 제39권8호
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• pp.619-624
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• 2016

Glioblastoma stem cells (GBM-SCs) are believed to be a subpopulation within all glioblastoma (GBM) cells that are in large part responsible for tumor growth and the high grade of therapeutic resistance that is so characteristic of GBM. MicroRNAs (miR) have been implicated in regulating the expression of oncogenes and tumor suppressor genes in cancer stem cells, including GBM-SCs, and they are a potential target for cancer therapy. In the current study, miR-203 expression was reduced in $CD133^+$ GBM-SCs derived from six human GBM biopsies. MicroRNA-203 transfected GBM-SCs had reduced capacity for self-renewal in the cell sphere assay and increased expression of glial and neuronal differentiation markers. In addition, a reduced proliferation rate and an increased rate of apoptosis were observed. Therefore, miR-203 has the potential to reduce features of stemness, specifically in GBM-SCs, and is a logical target for GBM gene therapy.

• 임상간호연구
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• 제20권3호
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• pp.348-358
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• 2014

Purpose: This was a descriptive correlation study to identify emergency room (ER) nurses' coping types and factors affecting compassion satisfaction (CS) and compassion fatigue. Methods: During March and April 2013, 170 questionnaires were distributed to ER nurses working in general hospitals in D and C regions. Data were analyzed using descriptive statistics, t-test, ANOVA, correlation analysis, and stepwise regression analysis. Results: Compassion fatigue was most strongly affected by emotional expression for burnout and by practical support pursuit for secondary traumatic stress. Positive coping, emotional expression, position, positive reinterpretation, and self-blame explained 42% of CS. Conclusion: Results indicate that nurses with moderate levels of career had high compassion fatigue and low compassion satisfaction. Thus these nurses need to be given an opportunity to achieve self-development and improve their ability as nurses; interventions that can reinforce positive coping strategies should be provided as personal coping strategies affect compassion fatigue and compassion satisfaction.