• Journal of fish pathology
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• v.19 no.1
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• pp.65-72
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• 2006

Nervous necrosis virus (NNV) that causes severe mortality during seed production of red drum (Sciaenops ocellatus) is known to be vertically transmitted from infected spawners. This study was conducted to produce NNV free seeds by testing spawners for NNV infection and using virus free eggs for seed production. RT-PCR analysis of 40 spawners showed 18 positives and 22 negatives. NNV was not detected from eggs obtained from the negative spawners but was detected from those obtained from the positives. NNV was not detected from culturing seawater in tanks and Chlorella spp., Brachionus plicatilis., and Brine shrimp those were provided as live feed. The survival rates of fry from NNV positive and negative spawners were 80% and 85%, respectively by two weeks after hatching. The mortality increased from 25days after hatching and the final survival rate of seeds from NNV positive and negative spawners were 0% and 18.3%, respectively on 41 days after hatching. These results suggested that virus free red drum seeds can be obtained by using virus-free spawners.

• Journal of Life Science
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• v.30 no.2
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• pp.178-185
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• 2020

Rice bakanae is a typical seed-borne and seed-transmitted disease caused by infection by Fusarium fujikuroi. Seed disinfection using chemical fungicides (such as benomyl and prochloraz) is most effective in controlling the disease, but the emergence of fungicide-resistant strains has recently been increasing. Slightly acidic hypochlorous acid water (SAHW) is a safe and environmentally friendly disinfectant that has a potent and broad spectrum of antimicrobial activity against viruses, bacteria, and fungi. In this study, we aimed to investigate the effectiveness of SAHW against F. fujikuroi strains, including chemical fungicide-resistant strains, as an alternative to conventional chemical fungicides in the management of bakanae disease. SAHW showed strong but similar levels of antifungal activity among the F. fujikuroi strains with a minimum inhibitory concentration (MIC₉₀) of 5±2.5 ppm of free available chlorine (FAC). In addition, F. fujikuroi cells lost viability completely within 5 min of SAHW treatment due to the lethal damage to cell integrity. When the rice seeds infected by F. fujikuroi were treated with SAHW containing 20±10 ppm of FAC for 12 hr, the efficiencies of seed disinfection and disease control were 95-98% and 90.1-92.6%, respectively. Altogether, our data suggest that SAHW is an effective compound for controlling rice bakanae disease.

• Research in Plant Disease
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• v.13 no.2
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• pp.82-87
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• 2007

The genetic diagnostic method of virion capture (VC)/RT-PCR was developed for the simultaneous detection of three rod shaped viruses of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus(KGMMV) and Zucchini green mottle mosaic virus (ZGMMV) transmitted by seed in Cucurbit. Out of 12 primer combinations for the three tobamoviruses, a primer set of CGMMV-C724, KGMMV-K513 and ZGMMV-Z407A was useful for mono and triplex VC/RT-PCR. The triplex VC/RT-PCR for the three tobamovirus in Cucurbit could detect specifically without interference among primers and/or plant species of watermelon, gourd, cucumber, melon, pumpkin, squash and Nicotiana benthamiana.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.41 no.11
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• pp.87-91
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• 2004

The continuing development of the information technology industry and wireless networking has increased the use of mobile device, which provides both portability and mobility. As follows, demands for extended services within the wireless Internet are increasing rapidly. Because it still in its initial stages of development, the wireless Internet presents continuing problems in security and limitations in the content of services. Furthermore, most mobile equipment utilizes the touch pad input method. This input method is inconvenient when a user needs to input a long sentence. This has led to the more convenient development of image selection by using a pen mouse. In order to provide security under these conditions, a HASH code may be used to transmit an array of information and input values, created by the image input at the early stages. Thus, authentication and key exchange are completed securely. Messages are encoded and transmitted, preventing both information drain by insiders and interference from outside.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.12
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• pp.815-821
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• 2015

Maritime safety has been more critical recently due to the occurrence of shipboard accidents involving prime movers. As such, the propulsion shafting design and construction plays a vital role in the safe operation of the vessel other than focusing on being cost-efficient. Smaller vessels propulsion shafting system normally install high speed four-stroke diesel engine with reduction gear for propulsion efficiency. Due to higher cylinder combustion pressures, flexible couplings are employed to reduce the increased vibratory torque. In this paper, an actual vibration measurement and theoretical analysis was carried out on a propulsion shafting with V18.3L engine installed on small car-ferry and revealed higher torsional vibration. Hence, a rubber-block type flexible coupling was installed to attenuate the transmitted vibratory torque. Considering the flexible coupling application factor, reduction gear stability due to torque variation was analyzed in accordance with IACS(International Association of Classification Societies) M56 and the results are presented herein.

• Journal of Korean Library and Information Science Society
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• v.29
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• pp.105-140
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• 1998

This is a way of establishing the information sovereignty in the industrial knowledge of society. The occurrence of the industrial knowledge of society builds knowledge and information as digital system. Digitalized knowledge and information has some characteristics of which the information should be light and variable, so that it can be transmitted rapidly. At this moment, if information super highway is built, and the digitalized information of the advanced information country comes to us, we are to get easily information dependence unless we are ready. Nowadays, our country is accepting the knowledge and information of the advanced information countries without filtering it properly. What is the reason? This means that we are lack of traditional knowledge resourses as the foundation of digital system. This paper deals with (1) the historical reason of the lackness of the traditional knowledge resourses, (2) the aspect of the consumer culture about knowledge and information, and (3) the aspect of the production culture about the knowledge and information. In order to overcome the information dependence, in this paper, we should change both production and consumption of our knowledge and information into a new pattern, establish the seed knowledge resourses, and build libraries everywhere we can use easily. For this case study, this paper takes examples of maeul (which may mean traditional villiage in Korea.) libraries on Changwon City.

• BMB Reports
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• v.57 no.2
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• pp.86-91
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• 2024

The fatty acids content of castor (Ricinus communis L.) seed oil is 80-90% ricinoleic acid, which is a hydroxy fatty acid (HFA). The structures and functional groups of HFAs are different from those of common fatty acids and are useful for various industrial applications. However, castor seeds contain the toxin ricin and an allergenic protein, which limit their cultivation. Accordingly, many researchers are conducting studies to enhance the production of HFAs in Arabidopsis thaliana, a model plant for oil crops. Oleate 12-hydroxylase from castor (RcFAH12), which synthesizes HFA (18:1-OH), was transformed into an Arabidopsis fae1 mutant, resulting in the CL37 line producing a maximum of 17% HFA content. In addition, castor phospholipid:diacylglycerol acyltransferase 1-2 (RcPDAT1-2), which catalyzes the production of triacylglycerol by transferring HFA from phosphatidylcholine to diacylglycerol, was transformed into the CL37 line to develop a P327 line that produces 25% HFA. In this study, we investigated changes in HFA content when endogenous Arabidopsis PDAT1 (AtPDAT1) of the P327 line was edited using the CRISPR/Cas9 technique. The successful mutation resulted in three independent lines with different mutation patterns, which were transmitted until the T4 generation. Fatty acid analysis of the seeds showed that HFA content decreased in all three mutant lines. These findings indicate that AtPDAT1 as well as RcPDAT1-2 in the P327 line are involved in transferring and increasing HFAs to triacylglycerol.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.4
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• pp.266-287
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• 2004

The non infecting, plant associated bacteria have attracted increased attention for stimulating plant growth and as environmental friendly plant protecting agents. Pink-pigmented facultatively methylotrophic bacteria (PPFMs), classified as Methylobacterium spp., are persistent colonizers of plant leaf surfaces. As the leaves of most or all plants harbor PPFMs that utilize leaf methanol as their sole source of carbon and energy, which is a specific attribute of the genus Methylobacterium. Although they are not well known, these bacteria are co-evolved, interacting partners in plant metabolism. This claim is supported, for example, by the following observations: (1) PPFMs are seed-transmitted, (2) PPFMs are frequently found in putatively axenic cell cultures, (3) Low numbers of seed-borne PPFMs correlate with low germinability, (4) Plants with reduced numbers of PPFM show elevated shoot/root ratios, (5) Foliar application of PPFMs to soybean during pod fill enhances seed set and yield, (6) Liverwort tissue in culture requires PPFM-produced vitamin B12 for growth, (7) treated plants to suppress or decrease disease incidence of sheath blight caused by Rhizoctonia solani in rice, and (8) the PPFM inoculation induced number of stomata, chlorophyll concentration and malic acid content, they led to increased photosynthetic activity. Methylobacterium spp. are bacterial symbionts of plants, shown previously to participate in plant metabolism by consuming plant waste products and producing metabolites useful to the plant. There are reports that inform about the beneficial interactions between this group of bacteria and plants. Screening of such kind of bacteria having immense plant growth promoting activities like nitrogen fixation, phytohormone production, alleviating water stress to the plants can be successfully isolated and characterized and integration of such kind of organism in crop production will lead to increased productivity.

• Journal of Digital Convergence
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• v.15 no.3
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• pp.221-228
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• 2017

With the recent development of IoT technology, medical services using IoT technology are increasing in many medical institutions providing health care services. However, as the number of IoT sensors attached to the user body increases, the healthcare information transmitted to the server becomes complicated, thereby increasing the time required for analyzing the user's healthcare information in the server. In this paper, we propose a deep learning based health care information management method to collect and process healthcare information in a server for a large amount of healthcare information delivered through a user - attached IoT device. The proposed scheme constructs a subnet according to the attribute value by assigning an attribute value to the healthcare information transmitted to the server, and extracts the association information between the subnets as a seed and groups them into a hierarchical structure. The server extracts optimized information that can improve the observation speed and accuracy of user's treatment and prescription by using deep running of grouped healthcare information. As a result of the performance evaluation, the proposed method shows that the processing speed of the medical service operated in the healthcare service model is improved by 14.1% on average and the server overhead is 6.7% lower than the conventional technique. The accuracy of healthcare information extraction was 10.1% higher than the conventional method.

• Korean journal of applied entomology
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• v.20 no.4 s.49
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• pp.191-195
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• 1981

Cucurbits including pumpkin (Cucurbita pepo), gourd (Lagenariaa siceraria), cucumber (Cucumis sativus), melon(Cucumis melo) and watermelon(Cucurbita anguria) were diseased with mosaic symptoms. The causal virus was identified as watermelon mosaic virus(WMV). The WMV was transmitted by Myzus persicae Sulzer, and no seed borne virus was found. The virus caused large local lesions on the inoculated leaves of the Chenopodium amaranticolor and mosaic symptom on the upper leaves of Cucumis melo, Cucumis sativus, Lagenaria siceraria, Cucurbita anguria and Cucurbita pepo. There were no symptoms on the inoculated leaves of the Nicotiana tabacum var. Bright yellow, Nicotiana glutinosa, Vigna unguiculata. Petunia hybrida and Datura stramonium. Thermal inactivation point was $55\~65^{\circ}C$, dilution end point was $10^{-4}\;10^{-5}$ and longevity in vitro of the virus was $7\~8$ days. The virus showed positive reaction against watermelon mosaic virus antiserum in microprecipitin tests. The virus particles were flexuous rods in size of 750 nm.