• 원예과학기술지
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• 제17권6호
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• pp.747-749
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• 1999

박종자의 발아율 향상과 발아촉진을 위한 종자처리시 hydropriming과 priming 처리와 종자의 발아성을 비교 검토하고, 종자처리 기술의 실용화를 위해 처리 화학물질 및 농도, 처리기간 및 처리온도, 발아온도에 따른 효과를 비교 검토하였다. 증류수(hydropriming)나 50mM $KH_2PO_4$와 50mM $KNO_3$로 $20^{\circ}C$에서 12시간 처리시 $T_{50}$ 및 평균발아소요일수 단축에 효과적 이었다. 무처리종자에 비하여 hydropriming이나 priming에 의한 발아촉진 효과는 발아온도 $25^{\circ}C$나 $35^{\circ}C$보다 $20^{\circ}C$에서 현저하게 나타났다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.88-88
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• 2018

In order to increase the germination speed and uniformity of tomato seeds, sonication and modified drum priming treatments were investigated to produce high quality seeds for export. Sonication treatment was performed for 5, 10 and 20minutes at an intensity of 5.2, 10.4 and 15.7kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $KNO_3$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 60, 72 and 84h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. Germination speed were highly improved by sonication with osmotic priming. The seed treatment of osmotic priming or hydro priming after sonication or sonication without priming enhanced germination percentage (GP) on the $2^{rd}$day after sowing to 46%, 43% and 28%, respectively, while untreated seeds resulted in only 1% GP. These treatments also highly improved mean germination time (MGT) to 1.4, 1.8 and 2.6days, respectively, when compared to 3.5days MGT of untreated seeds. The modified drum priming treatment (72h incubation after 60% SMC hydration) significantly improved results of 74% GP(on the $3^{rd}$day after sowing), 2.6days MGT and $39%{\cdot}day^{-1}$ germination rate (GR), however, untreated seeds showed 19% GP, 4.1 MGT, and $25%{\cdot}day^{-1}$ GR. Although osmotic priming after sonication, hydro priming showed similar improved germination characteristics, however, modified drum priming is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.

• 한국작물학회지
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• 제39권2호
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• pp.128-138
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• 1994

발아의 신속성과 균일성을 높이기 위하여 벼, 보리, 콩 종자에 대하여 몇가지 화학물질처리와 종자의 흡습건조처리를 통하여 종자퇴화지연효과를 검토하였다. 처리한 생장촉진제 및 노화지연제간에 경미한 차이는 있었으나 GA$_3$가 벼의 발아율을 높인것 외에는 뚜렷한 종자세 개선의 효과를 보이지 않았다. 노화종자를 PEG용액에 흡습건조처리할 경우 용액농도가 높을수록 평균발아일수가 길어지고 발아율이 낮아지는 경향이나 발아율 유지의 효과나 발아균일성향상의 효과는 없었다. 저온, 과습 및 삼투조건 등 불량한 환경에 대한 발아반응도 미미하였다. 어느 처리도 종자세 개선에 뚜렷한 효과를 보이지는 않았으나 GA$_3$ 처리가 전체적으로 발아반응이 그 중 좋은 편이었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.334-334
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• 2017

Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.53-53
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• 2017

Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

• 생물환경조절학회지
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• 제12권3호
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• pp.132-138
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• 2003

섬쑥부쟁이의 대량번식 체계를 구명하고 발아 및 입 묘율 향상을 위한 기초 자료를 얻기 위해 온도와 광조건, 그리고 priming 처리약제, 처리농도, 처리기간이 발아에 미치는 영향에 대하여 실험한 결과는 다음과 같았다. 온도에 따른 발아율은 20, 25$^{\circ}C$ 처리구가 가장 높았으며 30$^{\circ}C$에서 발아율이 급격히 낮아졌고 35$^{\circ}C$에서는 전혀 발아하지 않았다. 발아개시일은 15$^{\circ}C$에서 가장 늦어졌으며 온도가 높아질수록 빨라지는 경향을 보였으나 높은 온도에서는 쉽게 부패하였다. 발아세는 25$^{\circ}C$에서 가장 높게 나타났으며 15, 20, 30$^{\circ}C$ 순으로 낮이졌다. 굉조건 실험에서 발아율, 발아개시일, 발아세, $T_{50}$ 등이 명 처리에서 증진되는 경향을 보였으나 유의 차는 없었다 Priming 처리기간별로 발아율을 보면 평균적으로 3 시간 처리가 30 분처리보다 높았다. 섬쑥부쟁이 종자에 0.3M $KNO_3+K_3PO_4$를 3시간 처리할 경우 발아율이 83.3%로 가장 높았고 역시 $T_{50}$도 가장 짧았다. $T_{50}$과 발아개시일은 30분 처리가 3시간 처리보다 좋은 결과를 보였으며 모든 priming 처리가 대조구보다 좋은 결과를 보였다. 발아세는 모든 처리가 대조구보다 높았으나 처리시간에 따른 효과가 없었다.

• 한국작물학회지
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• 제62권4호
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• pp.373-381
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• 2017

The main objective of this study was to increase the germination percentage of kenaf seeds with less number of times under non-saline and saline conditions. Therefore, the first goal was to assess the response of kenaf seeds to NaCl. The second goal was to evaluate the effects of $KNO_3$ on kenaf seed germination. The germination percentage exhibited a decreasing tendency in germination rate. Plant dry weight was approximately 0.2 g in all treatments at 5 days after germination. As time passed, the electrical conductivity (EC) value of hydro-priming (HP) consistently increased by 8.7 mS/cm at 24 hours of immersion. However, seeds primed with $KNO_3$ showed no difference in EC values even as times passed. Regarding the priming effect, priming in 100 mM $KNO_3$ concentration for 12 hours increased germination up to 85% in $H_20$ solution and in 0 mM $KNO_3$ concentration upto 73.8% under 0.3% NaCl solution, compared to that of Control. Germination synchronization, shoot length, and leaf unfolding of primed seeds were greater than those of the Control. In addition, main root and hair roots appeared more rapidly in the treated seeds and were more abundant compared to that of the Control. The T50 (times to reach 50% of the final germination percentage) of the Control in both $H_20$ and 0.3% NaCl solutions was 18 and 22 hours, respectively. However, when treated $KNO_3$ priming (0 to 100 mM) in $H_20$ and 0.3% NaCl solution, 9 hours was sufficient to reach T50. Primed (hydro-priming and $KNO_3$) seeds had a lower MDG (mean days untill germination; 0.6-0.62) compared to that of the Control (1.13-1.31) in $H_20$ and 0.3% NaCl solutions. Regarding dry weight of plants after priming, an increasing tendency after the priming treatment in the H20 solution was observed. Furthermore, no significant difference in plant dry weight under 0.3% NaCl stress was observed between the Control and primed seeds. Taken together, the results suggest that 50-100 mM $KNO_3$ priming for 24 hours optimize seed germination rate in less number of times of exposure with great vigor. Therefore, it is recommended for kenaf seed invigoration before planting.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 춘계학술대회
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• pp.77-77
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• 2018

• 한국초지조사료학회지
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• 제26권4호
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• pp.277-284
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• 2006

종자의 priming은 유식물의 초기 정착에 중요한 기술 중의 하나이다. 따라서 본 시험에서는 목초의 주요 초종인 톨 페스큐, 오처드그라스, 알팔파 및 화이트 클로버의 발아율 향상과 균일도 증가를 위하여 priming 약제의 종류와 농도를 달리하여 발아율을 조사하였다. 본 시험은 분할구 배치법으로 주구는 $KNO_3,\;KH_2PO_4,\;K_3PO_4,\;Ca(NO_3){_2}$ 및 PEG 약제를, 세구는 50mM(또는 10%), 100mM(또는 20%) 및 200mM(또는 30%)의 약제농도를 두었다. Priming 목초의 발아율은 약제의 종류 및 농도에 따라 다르게 나타났다. 화본과 목초인 톨 페스큐는 $Ca(NO_3){_2}$와 $K_3PO_4$가 다른 약제보다 발아율이 높았으며, 오처드그라스는 약제간에 차이가 없었으나 $KH_2PO_4$가 다른 약제보다 발아율이 높았다. 한편 두과목초인 알팔파와 화이트 클로버는 모두 PEG에서 높은 발아율을 나타내었다. 한편 약제의 농도는 약제의 종류에 따라 달랐다. 이상의 결과를 종합해 보면 목초의 priming은 초종에 따라 적정 약제종류와 농도가 달랐다.

• 아시안잔디학회지
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• 제8권1호
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• pp.1-11
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• 1994

Studies were conducted to evaluate the effect of priming and preimbibition on gerraination of unscarified and KOH-scarified zoysiagrass (Zoysia japonica Steud.) seeds, respectively. Germi-nation percentages at 35˚C of seeds primed in $KNO_3$ and Ca($NO_3$)$_2$ were significantly higher than that of untreated seeds. The optimum conditions for priming unscarified seeds were in aerated $KNO_3$ solution at O4 M for 4 days at 20˚C. No additional benefit of $GA_3$ on increasing germination was not found in seed priming with solution of $KNO_3$ + $GA_3$ or in seeds preimbibed with $GA_3$ after priming. Scarified seeds preimbibed with distilled water(DW) for 4 days at 20˚C germinated higher and with fewer days to 50% of total germination(T 50) than untreated seeds. Preimbibition with $GA_3$ solution at 50 ppm for 4 days at 5, 10, 15 and 20˚C enabled up to 97% of seeds to germinate and reduced days to T 50 about 1.1 to 1.2 days at 35˚C Especially, seeds preimbibed with $GA^2$˚$_3_2_1$$\times$ had greatly higher germination and lower T 50 than untreated or DW-preimbibibed seeds at 30˚C, which is inadequate to germination in general. Preimbibed seed performance was unchanged after storage at room temperature for 50 days. Seeds preimbibed with GA $_3$ had higher rate of seedling emergence and faster growth of shoot than untreated seeds after sowing at 30˚C in growing medium in growth chamber.