• Journal of Life Science
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• v.28 no.4
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• pp.454-464
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• 2018

Black soybeans are used as food sources as well as for traditional medicines because they contain an abundance of natural phenolic compounds. In this study, total phenolic contents (TPCs) of Korean black seed coat soybean varieties Socheongja (SCJ), Socheong 2 (SC2) and Cheongja 2 (CJ2) as well as their antioxidant capacities were investigated. Among them, TPCs were abundantly present in the order of CJ2$H_2O_2$-stimulated HaCaT human keratinocytes. Our results revealed that treatment with SCJ and SC2 prior to $H_2O_2$ exposure significantly increases the viability of HaCaT cells, indicating that the exposure of HaCaT cells to SCJ and SC2 conferred a protective effect against oxidative stress. SCJ and SC2 also effectively inhibited $H_2O_2$-induced apoptotic cell death through the blocking of mitochondrial dysfunction. SCJ and SC2 also attenuated the phosphorylation of Histone H2AX. Furthermore, they effectively induced the levels of thioredoxin reductase (TrxR) 1, a potent antioxidant enzyme, which is associated with the induction of nuclear transcription factor erythroid-2-like factor 2 (Nrf2); however, the protective effects of SCJ and SC2 were significantly reversed by Auranofin, a TrxR inhibitor. These results indicate that they have protective activity through the blocking of cellular damage related to oxidative stress via the Nrf2 signaling pathway. In conclusion, our study indicated that SCJ and SC2 might potentially serve as novel agents for the treatment and prevention of skin disorders caused by oxidative stress.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.333-345
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• 2017

The potato tuber is known as a rich source of essential nutrients, used throughout the world. Although potato-breeding programs share some priorities, the major objective is to increase the genetic potential for yield through breeding or to eliminate hazards that reduce yield. Glycoalkaloids, which are considered a serious hazard to human health, accumulate naturally in potatoes during growth, harvesting, transportation, and storage. Here, we used the AMMI (additive main effects and multiplicative interaction) and GGE (Genotype main effect and genotype by environment interaction) biplot model, to evaluate tuber yield stability and glycoalkaloid content in six potato cultivars across three locations during 2012/2013. The environment on tuber yield had the greatest effect and accounted for 33.0% of the total sum squares; genotypes accounted for 3.8% and $G{\times}E$ interaction accounted for 11.1% which is the nest highest contribution. Conversely, the genotype on glycoalkaloid had the greatest effect and accounted for 82.4% of the total sum squares), whereas environment and $G{\times}E$ effects on this trait accounted for only 0.4% and 3.7%, respectively. Furthermore, potato genotype 'Superior', which covers most of the cultivated area, exhibited high yield performance with stability. 'Goun', which showed lower glycoalkaloid content, was the most suitable and desirable genotype. Results showed that, while tuber yield was more affected by the environment, glycoalkaloid content was more dependent on genotype. Further, the use of the AMMI and GGE biplot model generated more interactive visuals, facilitated the identification of superior genotypes, and suggested decisions on a variety of recommendations for specific environments.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.144-155
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• 1987

Water extracts of polygonum hydropiper and Polygonum aviculare completely inhibited the germination of lettuce seeds. Methanol extracts from these two species also inhibited the seed germination of lettuce (Lactuca sativa) and Oenothera odorata. Fifteen phenolic acids in total were identified by GLC from P. hydropiper and eighteen from P. aviculare. The most common phenolic acids identified from P. hydropiper were sinapic, salicylic+vanillic and ferulic acid presented in all the fractions. In addition, salicylic+vanillic, tannic+gallic, sinapic, ferulic and p-coumaric acid seemed to be important phenolic compounds in terms of quantity. However, salicylic+vanillic acids were the unique phenolic acids occurred in all the fractions of P. aviculare. The others such as tannic+gallic, sinapic, ferulic, p-coumaric acid, p-cresol and catechol present in large amount appeared also the important phenolic substances influencing allelopathic effects of P. aviculare. Linolenic acid and oxalic acid were the major fatty and organic acids in both plant species, presented in 2.38mg/g and 20.588mg/g in P. hydropiper, 3.70mg/g and 14.288mg/g in P. aviculare, respectively, which seem to be exhibiting allelopathic effects of these plants. Total alkaloids were presented in low amount such as 0.20% in P. hydropiper arid 0.22% in P. aviculare which may not be important elements. Pet. ether extracts were 2.42% in P. hydropiper and 1.65% in P. aviculare, which exhibit another potential for allelopathic effects that need further investigation. Various authentic phenolic compounds at different concentrations inhibited the germination of lettuce seed, indicating that the phenolic substances identified here may be directly related to biologically active substance.

• Korean Journal of Plant Resources
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• v.32 no.5
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• pp.423-432
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• 2019

In order to select potential plant resources as functional materials and natural antioxidants, we evaluated antioxidant activity and serotonin derivatives of safflower germplasm collected from five countries. N-(p-Coumaroyl) serotonin (CS) and N-feruloylserotonin (FS) were analyzed by using Ultra Performance Liquid Chromatography (UPLC). Total polyphenol content (TPC) was determined by Folin-Ciocalteu method and antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), Ferric reducing antioxidant power (FRAP) and Reducing power (RP) assays. The TPC showed a range of 28.25 to $90.53{\mu}g$ gallic acid equivalent (GAE)/mg dried extract (DE). DPPH, ABTS, FRAP and RP assay were in the range of 18.76 to 93.98, 48.91 to 163.73, 3.80 to 132.29 and 26.32 to $80.08{\mu}g$ ascorbic acid equivalent (ASCE)/mg DE, respectively. Among the five countries, safflower seed collected from Iran had the highest levels of serotonin derivatives and antioxidant activities than other countries (p<0.05). CS showed high correlation with TPC, ABTS and DPPH (r=0.673,0.727,0.820), and FS showed high correlation with DPPH (r=0.740). Accessions IT321214 and IT321215 could be useful for development of new functional materials and could be used as a source of valuable natural antioxidant materials.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.512-516
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• 2008

A new naked oat cultivar 'Choyang' (Avena sativa L.) was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2007. It was derived from a cross between 'Sikyonggwiri' and 'Gwiri23'. The cultivar, Sikyonggwiri is early heading while the breeding line Gwiri23 has a high yielding potential with large-size grain. Bulk method combined with pedigree selection program was employed in subsequent generations, and the promising line SO96025-B-303-44-2-5 was selected for agronomic performance in 2002. The line showed both high yield and good husking rate of seed in the Yield Trial tested at Suwon in 2003 to 2004, and was subsequently designated as 'Gwiri57'. Gwiri57 was evaluated for winter hardiness, earliness, and yield in four locations, Gimje, Iksan, Jeongeup and Jinju from 2005 to 2007 and was released as Choyang. Choyang headed 11 days earlier and matured 5 days earlier than the check cultivar 'Sunyang' in the paddy field condition. The new cultivar Choyang had 97 cm of culm length and 23.4 cm of spike length, 658 spikes per $m^2$, 75 grains per spike, 28.8 g of 1,000-grain weight, and 636 g of test weight. Choyang showed better winter hardiness than that of the check cultivar Sunyang, and similar seed quality to the check cultivar in respect to crude protein (12.9%) and ${\beta}$-glucan content (4.7%). However, it showed higher husking rate than the check cultivar. The grain yield of Choyang in the regional yield trial for 3 consecutive years was averaged $4.67MT\;ha^{-1}$, which was 38% higher than that of the check cultivar Sunyang. Choyang is recommended for the fall sowing cropping only in the southern area where daily minimum mean temperatures are averaged higher than $-4^{\circ}C$ in January, and is not recommended the in mountain area where frost damage is presumable.

• Polymer(Korea)
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• v.32 no.2
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• pp.103-108
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• 2008

Osmotic pellet, which consisted of water-swellable seed layer, drug layer, and porous membrane layer, has been widely utilized in oral drug delivery system. In this work, we describe the preparation of osmotic pellet with nifedipine as model drug and a mixture of cellulose acetate (CA) and Eudragit RS as membrane layer, and then examined the drug release behavior on the variation of the thickness change of membrane layer (CA and Eudragit RS) and release media. Furthermore, we examined the nifedipine release behavior using sodium alginate as a potential membrane candidate. Osmotic pellet was obtained in the quantitative yield by fluidized bed coater. Osmotic pellet exhibited the round morphology and the size ranging $1500{\sim}1700{\mu}m$ in SEM. The nifedipine release decreased as the thickness of membrane layer (CA and Eudragit RS) increased. In addition, it observed that there is difference of release amount in between intestinal juice (pH 6.8) and gastric juice (pH 1.2). In the case of osmotic pellet coated with sodium alginate, nifedipine release behavior depended on the crosslinking of sodium alginate layer. In conclusion, we found that various membrane layers could control the release amount of nifedipine.

• Journal of Bio-Environment Control
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• v.21 no.3
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• pp.199-206
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• 2012

This study was conducted in a glasshouse to examine the possibility of producing tomato plug seedlings in a newly-developed inert phenolic foam medium. Plug seedlings of 'Madison' tomato were grown in four pellet type media, Grodan rockwool, UR rockwool, phenolic foam LC, and phenolic foam LC-lite. Seed germination was checked for 7 days. Seedling growth was measured at 19 days after sowing. The greatest germination was obtained in the phenolic foam LC and phenolic foam LC-lite. Plant height, hypocotyl length, leaf area, dry weight, and fresh weight were significantly greater in the rockwool medium than those in the other media. However, the T/R ratio and stem diameter were the greatest in the phenolic foam LC than those in the other media. The total porosity and container capacity of the phenolic foam LC was higher than in the other media. The air space (%) was lowest in the phenolic foam LC. Overall, the phenolic foam LC and phenolic foam LC-lite produced seedlings with similar growth as the rockwool. These results suggested that both phenolic foam LC and phenolic foam LC-lite have potential to be used in production of plug seedlings of 'Madison' tomato.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.40-44
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• 2003

Soil-borne infectious disease including Pythium aphanidermatum and Rhizoctonia solani causes severe damage to plants, such as cucumber. This soil-borne infectious disease was not controlled effectively by chemical pesticide. Since these diseases spread through the soil, chemical agents are usually ineffective. Instead, biological control, including antagonistic microbe can be used as a preferred control method. An efficient method was developed to select an antagonistic strain to be used as a biological control agent strain. In this new method, surface tension reduction potential of an isolate was included in the ‘decision factor’ in addition to the other factors, such as growth rate, and pathogen inhibition rate. Considering these 3 decision factors by a statistical method, an isolate from soil was selected and was identified as Bacillus sp. GB16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth was observed when Bacillus sp. GB16 was used. Therefore this strain was considered as plant growth promoting rhizobacteria (PGPR). The action of surface tension reducing component was deduced as the enhancement of wetting, spreading, and residing of antagonistic strain in the rhizosphere. This result showed that new selection method was significantly effective in selecting the best antagonistic strain for biological control of soil-borne infectious plant pathogen. The antifungal substances against P. aphanidermatum and R. solani were partially purified from the culture filtrates of Bacillus sp. GB16. In this study, lipopeptide possessing antifungal activity was isolated from Bacillus sp. GB16 cultures by various purification procedures and was identified as a surfactin-like lipopeptide based on the Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), high performance liquid chromatography mass spectroscopy (HPLC-MS), and quadrupole time-of-flight (Q-TOF) ESI-MS/MS data. The lipopeptide, named GB16-BS, completely inhibited the growth of Pythium aphanidermatum, Rhizoctonia solani, Penicillium sp., and Botrytis cineria at concentrations of 10 and 50 mg/L, respectively. A novel method to prevent the foaming and to provide oxygen was developed. During the production of surface active agent, such as lipopeptide (surfactin), large amount of foam was produced by aeration. This resulted in the carryover of cells to the outside of the fermentor, which leads to the significant loss of cells. Instead of using cell-toxic antifoaming agents, low amount of hydrogen peroxide was added. Catalase produced by cells converted hydrogen peroxide into oxygen and water. Also addition of corn oil as an oxygen vector as well as antifoaming agent was attempted. In addition, Ca-stearate, a metal soap, was added to enhance the antifoam activity of com oil. These methods could prevent the foaming significantly and maintained high dissolved oxygen in spite of lower aeration and agitation. Using these methods, high cell density, could be achieved with increased lipopeptide productivity. In conclusion to produce an effective biological control agent for soil-borne infectious disease, following strategies were attempted i) effective screening of antagonist by including surface tension as an important decision factor ii) identification of antifungal compound produced from the isolated strain iii) novel oxygenation by $H_2O_2-catalase$ with vegetable oil for antifungal lipopeptide production.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.651-654
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• 2011

Cymbidium 'Orange Bowl' (Lucky Rainbow 'Randevous' ${\times}$ 'Eastern Star') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made between the pink colored flower C. Lucky Rainbow 'Randevous' as maternal line and pure yellow colored flower, C. 'Eastern Star' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2006. The line was named as Wongyo F1-18 and phenotype was characterized in 2006 as a new 'Orange Bowl'. The 'Orange Bowl' has having light yellow basal color (RHS, YO21D) and orange line (RHS, OR30B) on both of sepal and petal with red lip (RHS, OR30B). 'Orange Bowl' has about 10.9 flowers per flower stalk and flower size of 7.4 cm. General appearance of the petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid winter) under optimal culture condition. Leaf attitude and twisting is half- erect and very weak respectively. This hybrid has attractive floral arrangement, long flower stalk (71.8 cm) and vigorous growth. We expect that 'Orange bowl' has a great potential for exporting to Chinese market.

• The Korean Journal of Pesticide Science
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• v.13 no.4
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• pp.275-282
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• 2009

The effect of two plant growth-promoting rhizobacteria (PGPR) on plant growth and systemic protection against soft rot disease and stem rot disease of canola (Brassica napus), caused by Erwinia carotovora and Sclerotinia sclerotiorum was investigated in a laboratory and a greenhouse. Selected PGPR strains C4 and D8 were treated to canola seeds by soaking. Strains C4 and D8 significantly not only increased plant height and root length about 74% and 40.3% and also reduced disease severity of soft rot disease by 80% by C4 and D8 respectively, compared to the control. Especially strain C4 showed antifungal activity against 6 fungal pathogens, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Colletotrichum acutatum. In greenhouse experiment, the seed treatment of both of them increased plant height, leaf width and leaf length of canola plant to 19.5% and 24.9%, 11.3% and 15.3%, and 14.1% and 20.7% by C4 and D8, respectively, and reduced disease severity of S. sclerotiorium. These results indicate that these two PGPR strains can decrease disease severity and increased plant growth under greenhouse condition. Therefore, these two bacteria have a potential in controlling Sclerotinia stem rot of canola. These strains have to investigate under field condition to determine their role of antibiosis, induced systemic resistance and plant growth promotion on canola.