• Journal of Applied Biological Chemistry
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• v.53 no.2
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• pp.77-81
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• 2010

The seed of cowpea (Vigna sinensis K.) was extracted with 80% aqueous methanol (MeOH). And the concentrated extract was partitioned with ethyl acetate (EtOAc), n-butanol (n-BuOH) and $H_2O$, successively. The repeated silica gel and octadecyl silica gel (ODS) column chromatographic separations for the EtOAc and n-BuOH fractions led to isolation of four sterols. And the chemical structures of the compounds were determined as a mixture of stigmasterol and $\beta$-sitosterol with the ratio of 4 to 3 (1), 7-ketositosterol (2), and stigmasterol 3-O-$\beta$D-glucopyranoside (3) from the interpretation of spectroscopic data including nuclear magnetic resonance (NMR) spectrum metric, mass (MS) spectrum metric and infrared (IR) spectroscope. This study reports the first isolation of $\beta$-sitosterol, 7-ketositosterol, and stigmasterol 3-O-$\beta$-D-glucopyranoside from the seed of Vigna sinensis K. In addition, compound 2, 7-ketositosterol, is rarely occurred in natural source including plant.

• Horticultural Science & Technology
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• v.30 no.5
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• pp.573-578
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• 2012

A total of eleven Chinese cabbage accessions were used for microspore culture and were grown to take basal data. Based on the collected data, breeding materials were chosen to develop new improved Chinese cabbage cultivars. The range of microspore-derived embryoid taken from flower buds was 1.6 to 35.4 embryoids. The embryoids from IT26110 and IT26153 among the Chinese cabbages were more than 34 per flower bud. The viability rate after cold treatment was low from 0.2 to 11.7%. The range of fertility rate was 7.7 to 58.8% in general but the IT26118, IT26122, IT26128, IT26130, and IT26164 were more than 50%. The result of their seed production ability by selfing was 11.9 seeds per siliqua in IT26128 while the others were less than 10 seeds. In the microspore culture using parents of different hereditary, the number of embryoids, the number of plants, the rate of fertility and their pure seed production ability appeared to be very different in doubled haploid lines obtained from fertile plants of Chinese cabbage.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.121-126
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• 2012

To determine whether FT-IR spectroscopy combined with multivariate analysis for whole cell extracts can be used to discriminate major leguminous plant at metabolic level, seed extracts of six leguminous plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from seed extracts were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). The PCA could not fully discriminate six leguminous plants, however PLS-DA could successfully discriminate six leguminous plants. The hierarchical dendrogram based on PLS-DA separated the six leguminous plants into four branches. The first branch was consisted of all three Vigna species including Vigna radiata var. radiate, Vigna angularis var. angularis and Vigna unguiculata subsp. Unguiculata. Whereas Pisum sativum var. sativum, Glycine max L and Phaseolus vulgaris var. vulgaris were clustered into a separate branch respectively. The overall results showed that metabolic discrimination system were in accordance with known phylogenic taxonomy. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from seed extracts represented the most probable chemotaxonomical relationship between six leguminous plants.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.580-589
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• 2013

This study was carried out to evaluate the suitability of microsatellite markers for varietal identification and genetic relationship of 170 commercial pepper cultivars. The relationship between marker genotypes and 11 pepper cultivars with different morphological traits was also analyzed. Of the 302 pairs of microsatellite primers screened against 11 pepper cultivars, 24 pairs were highly polymorphic in terms of number of alleles. These markers were applied for the construction of DNA profile data base for 170 commercial pepper cultivars. A total of 164 polymorphic amplified fragments were obtained from 24 microsatellite primers. The average polymorphism information content was 0.673 ranging from 0.324 to 0.824. One hundred and sixty four microsatellite alleles were used to calculate Jaccard's distance coefficients using unweighted pair group method. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into 3 major groups corresponding to morphological traits. The phenogram discriminated all varieties by markers genotypes. These microsatellite markers will be useful as a tool for protection of plant breeders' intellectual property rights through variety identification in distinctness, uniformity and stability test.

• Journal of KIISE
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• v.42 no.4
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• pp.504-511
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• 2015

Recently, the amount of data is rapidly increasing with the popularity of the SNS and the development of mobile technology. So, it has been actively studied for the effective data analysis schemes of the large amounts of data. One of the typical schemes is a Voronoi diagram based on kNN join algorithm (VkNN-join) using MapReduce. For two datasets R and S, VkNN-join can reduce the time of the join query processing involving big data because it selects the corresponding subset Sj for each Ri and processes the query with them. However, VkNN-join requires a high computational cost for constructing the Voronoi diagram. Moreover, the computational overhead of the VkNN-join is high because the number of the candidate cells increases as the value of the k increases. In order to solve these problems, we propose a MapReduce-based kNN-join query processing algorithm for analyzing the large amounts of data. Using the seed-based dynamic partitioning, our algorithm can reduce the overhead for constructing the index structure. Also, it can reduce the computational overhead to find the candidate partitions by selecting corresponding partitions with the average distance between two seeds. We show that our algorithm has better performance than the existing scheme in terms of the query processing time.

• Korean Journal of Construction Engineering and Management
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• v.18 no.3
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• pp.63-73
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• 2017

In contemporary society, it is increasingly common to spend more time indoors. As such, there is a continually growing desire to build comfortable and safe indoor environments. Along with this trend, however, there are some serious indoor-environment challenges, such as the quality of indoor air and Sick House Syndrome. To address these concerns the government implements various systems to supervise and manage indoor environments. For example, green building certification is now compulsory for public buildings. There are three categories of green building certification related to indoor air in Korea: Health-Friendly Housing Construction Standards, Green Standard for Energy & Environmental Design(G-SEED), and Indoor Air Certification. The first two types of certification, Health-Friendly Housing Construction Standards and G-SEED, evaluate data in a drawing plan. In comparison, the Indoor Air Certification evaluates measured data. The certification using data from a drawing requires a considerable amount of time compared to other work. A 2D tool needs to be employed to measure the area manually. Thus, this study proposes an automatic assessment process using a Building Information Modeling(BIM) model based on 3D data. This process, using open source Industry Foundation Classes(IFC), exports data for the certification system, and extracts the data to create an Excel sheet for the certification. This is expected to improve the work process and reduce the workload associated with evaluating indoor air conditions.

• Korean Journal of Geomatics
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• v.5 no.1
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• pp.27-34
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• 2005

Unsupervised classification is an important area of research in image processing because supervised classification has the disadvantages such as long task-training time and high cost and low objectivity in training information. This paper focuses on unsupervised classification, which can extract ground object information with the minimum 'Spectral Angle Distance' operation on be behalf of 'Spectral Euclidian Distance' in the clustering process. Unlike previous studies, our algorithm uses the unit vector, not the spectral distance, to compute the cluster mean, and the Single-Pass algorithm automatically determines the seed points. Atmospheric correction for more accurate results was adapted on the Hyperion data and the results were analyzed. We applied the algorithm to the Hyperion and ETM+ data and compared the results with K-Means and the former USAM algorithm. From the result, USAM classified the water and dark forest area well and gave more accurate results than K-Means, so we believe that the 'Spectral Angle' can be one of the most accurate classifiers of not only multispectral images but hyperspectral images. And also the unit vector can be an efficient technique for characterizing the Remote Sensing data.

• Journal of the Korean Society of Radiology
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• v.17 no.1
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• pp.149-156
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• 2023

The purpose of this study is to compare of growth and chlorophyl between old-barley seed (OBS, 2019) and New-barley seed (NBS, 2020) exposed by X-ray. After germination the OBS and NBS, experimental group was exposed by 30 Gy X-ray using linear accelerator (Clinac IS, VERIAN, USA), by 6 MV X-ray, SSD 100 cm, 18 × 10 cm², 600 MU/min. Length was measured every day until 9th day, and chlorophyl was analyzed using spectrophotometer(uv-1800, shimadzu, japan) after measuring weight in 9th day. Data analysis was performed the Independent T-test using SPSS ver 26.0(Chicago, IL, USA). NBS grow more faster than OBS in control group, but OBS grow more faster than NBS in experimental group. Length of control group was longer significantly every day than that of experimental group in OBS. NBS weighted more than OBS in control group, but OBS weighted more than NBS in experimental group. In comparing chlorophyl density, NBS high more than OBS in control group as well as experimental group. Growth and weight of OBS was effected more those than NBS by X-ray, but NBS in chlorophyl by X-ray. It is expected to be used as basic data for future X-ray research in barley seed.

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.378-390
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• 1997

Ten total populations of Korean fir (Abies koreana Wilson) and Manshurian fir [A. nephrolepis (Traut.) Maxim.] were sampled from south Korea to investigate patterns of intraspecific variation in these species and to evaluate a recognition of the two species. Principal components analysis and cluster analysis were performed both on seed-cone data and on needle morphology data. The characters that contributed most to the separation between A. koreana and A. nephrolepis along three principal components axis were leaf width, length of seed, width of seed wing, length of seed wing, cone width, width of scale, and length of bract tip, but these characters were not diagnostic because of overlap in reality. Therefore, all these characters were not reliable in distinguishing these two taxa including bract position (exerted and recurved vs. exerted and straight). The individuals of A. koreana from Mt. Chi-ri appeared quite unique probably on account of its larger cone size and longer scale tip, while those from Mt. Hal-la of A. koreana were generally distinct from others in terms of their larger seed and seed wing and longer scale width. The Mt. Duk-yu specimens of A. korecana appeared somewhat smaller but more data were needed due to the small sampling size. Generally, the gradual clinal geographic trends made evident by the position of resin ducts in leaves of A. koreana can be detected. The southern populations, Mt. Hal-la (an insular population) were generally distinct from the northern populations (Mt. Chi-ri, Mt. Ga-ya and Mt. Duk-yu) in terms of their position of resin duct (medial, within mesophyll vs marginal, close to epidermis : 100% vs 75 or 50%). Although no sharp boundary separating these two species could be detected based on cone and needle morphology, the observed clinal pattern was distinct in northern populations of A. koreana and southern population of A. nephrnlepis. In a preceding study of the flavonoids variation of 20 species in eastern Asia, flavanone (5-deoxyflavanone) was found to be characteristic of A. faxoniana Rehder et Wilson, A. georgei Orr of China and A. koreana of Korea. A. faxoniana, which is assumed to be primitive species, has position of resin duct relative to both the medial and the marginal, while A. georgei and A. koreana are identified by marginal position of resin duct. With respect of foliar flavonoids chemistry, A. koreana was distinct from A. nephrolepis : the southmost samples (Mt. Hal-la and Mt. Chi-ri) contained additional flavonoids derivatives (mainly flavanone) that were not found in the northmost samples of A. nephrolepis except a few individuals from Mts. Seo-rak and Tae-bak populations of Kwang-won province. The presence of A. koreana type flavonoids in two Chinese species suggested that position of resin duct may be a phyletic character. Abies koreana including two Chinese taxa, exhibited the most elaborate and specialized flavonoids profile within the Abies in eastern Asia. Contrary to our initial expectations, the apparent intermediates between A. nephrolepis and A. koreana in Duk-yu and Ga-ya mountains were found. The pattern of variation on position of resin duct and flavonoids chemistry in these populations of A. kareana suggested that genetic interchange or natural hybridization had occurred between these two species. The evidence needed to resolve the status of this taxon is still inconclusive in our opinion until intermediate individuals from Mts. Duk-yu and Ga-ya show indication of hybridization between the two species.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.04a
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• pp.141-150
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• 2000

In this study liquefaction hazard potential was assessed by modified Seed and Idriss method and maps of liquefaction hazard potential utilized by LPI(Liquefaction Potential Index) and FE(Equivalent Liquefaction Factor of Safety) were constructed in two dimensional space, Comparisons of liquefaction hazard maps assessed by LPI and FE are represented to verify the FE method proposed in this study. Based on the results of comparing liquefaction hazard map using LPI and FE there is similar distribution trend of zonation indices. from the result of comparison of liquefaction hazard maps of FE base using Hachinohe and ofunato PGA(Peak ground Acceleration) data at one site of port and harbor in Korea the values of FE in liquefaction hazard map using Hachinohe data are underestimated. And in the view of quantitative analysis FE is more convenient than LPI because types of results from FE are factor of safety that widely used in geotechnical practice and aseismic design standard for port and harbor in Korea.