• 한국수산과학회지
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• 제54권6호
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• pp.1007-1016
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• 2021

This study examined the characteristics and seasonal distribution patterns of subtidal marine algal communities along the east coast of Korea, from 2016 to 2017, and recorded the coverage ratio (%) by the algal type. During the study period, 134 marine algae species (16 green, 29 brown, and 89 red) were identified at the survey sites. The maximum number of species was observed at the Nagok site (70 species), and the minimum at the Gisamoon site (50 species). The total average biomass (dry weight) during the survey period was 66.22 g/m², including green algae (2.19 g/m²), brown algae (38.10 g/m²), and red algae (25.94 g/m²). The dominant seaweeds in the vertical distribution based on coverage rates (%) were Sargassum yezoense and Dictyopteris divaricata at the upper, Symphyocladia latiuscula and Undaria pinnatifida at the middle, and Agarum clathratum subsp. yakishiriense and Plocamium telfairiae at the lower sections of the distribution. Richness, evenness, and diversity index, calculated based on the biomass of the abundant species, were estimated to be 6.36, 0.55, and 1.98, respectively, over the entire sea area. Based on the evaluation of the environmental states using community indices, the ecological evaluation index (EEI-c) of subtidal marine algal communities along the east coast of Korea was marked as 'good-moderate'.

• 한국수산과학회지
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• 제40권5호
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• pp.323-331
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• 2007

Barren ground is an abnormal phenomenon in coastal ecosystems where seaweeds are destroyed and largely replaced by coralline algae containing calcium carbonate components. To restore the seaweed forest, we attempted reconstruction in an area of Samcheok, Korea, where barrenness is extensive. We developed two methods of seaforest construction underwater longline and seed transplantation for the brown seaweed Laminaria japonica, a fast-growing edible seaweed. The blade length of L. japonica attached to an underwater long line was $93.9{\pm}38.2cm$ and blade weight $7.6{\pm}2.1g$ in February 2004. Seaweed size declined after August 2004. A Similar pattern was observed using the transplantation method with maxima of $179.3{\pm}40.3cm\;and\;14.9{\pm}3.2g$ for blade length and weight, respectively in July 2004. The transplanted seaweeds matured in October as did a wild population. These results indicate that transplanted seaweed acclimate to new environments.

• 한국해양학회지:바다
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• 제21권1호
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• pp.44-47
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• 2016

Real-time PCR을 적조출현주의보 발령에 활용하였다. Cochlodinium polykrikoides 적조예보를 위해 $10cells\;mL^{-1}$ 이상에서 적조출현주의보를 발령하였는데 현미경 검경은 저밀도에서 정확도가 낮기 때문에 이를 보완하기 위해 C. polykrikoides 특이적 real-time PCR을 사용하였다. 6월 말경에 C. polykrikoides이 통영, 남해 해역에 저밀도로 광범위하게 출현하였고 2015년 8월 2일 적조출현주의보를 발령하였다.

• 한국수산과학회지
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• 제36권4호
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• pp.365-371
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• 2003

The reared fries of black sea bream, Acanthopagrus schlegeli were released in Gamak Bay and initial adaptation to the environment was evaluated. Thirty thousand fries were marked with magnetic tag, released and recaptured and its stomach content were analysed; the neighboring zooplankton, beuthic algae and other young fishes were investigated. The fries were released on July 30, 2001 in summer season. Water temperature of surface and bottom were $24^{\circ}C\;and\;21^{\circ}C,$ respectively. $Chlorophyll\;\alpha$ content was 4.5 ${\mu}g/L$ with high primary production. Salinity was $32.0-32.5\%_{\circ}.$ Water depth of releasing site was about 30 m. The sea jungle was formed at the first point of releasing and it kept the fries for about 3 days in the bay with plenty of food organisms within 5 m depth. Stomach analysis of the fries revealed that real feeding started from the 15 days after releasing and full feeding took place after the tenth day.

• KSBB Journal
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• 제26권6호
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• pp.538-542
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• 2011

The research was purposed production of oligosaccharide from alginate hydrolysis the main composition in cell walls of sea weed. We was isolated 252 strains from sea water and mud flat, the highest alginate lyase activity was selected, and identified as Sanguibacter keddieii NC9 by 16S rDNA sequence analysis. In this study was select the sodium alginate concentration, pH, temperature for the production of alginate lyase activity. Alginate lyase activity was confirmed from plate assay with 10% cetylpyridinium chloride. The optimum culture conditions for the production of alginate lyase were sodium alginate 10 g/L, peptone 5 g/L, $40^{\circ}C$, pH 9 and 36 hours incubation time. Sanguibacter keddieii NC9, its alginate lyase would be useful for the production of bioenergy and biofunctional oligosaccharides from sea weed.

• 한국해양바이오학회지
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• 제15권1호
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• pp.24-32
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• 2023

The Laminaria japonica Aresch (Sea tangle) belongs to the brown algae and has a long history as a food material in Asia, including Korea. Recent studies have found that the fermented Sea tangle extract (FST) inhibited the differentiation of osteoclasts and protected osteoblasts from oxidative damage. This study aims to explore the possibility that FST can induce the differentiation of osteoblasts and identify the responsible mechanism. According to our results, FST induced differentiation into osteogenic cells in the presence of osteoblastic MC3T3-E1 cells under non-toxic conditions.. This finding was confirmed by phalloidin staining, increased alkaline phosphatase activity, and calcium deposition. Additionally, it was found that this process was achieved by increasing the expression of key factors involved in osteoblast differentiation, such as runt-related transcription factor-2, osterix, β-catenin, and bone morphogenetic protein-2. Moreover, FST increased autophagy, which may contribute to the maintenance of the bone formation homeostasis, and is associated with the activation of the phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase signaling pathways. Although further research about the bioactive substances contained in FST and the tests of their efficacy are required, the results of this study indicate that FST has incredible applicability as a functional material for maintaining the bone homeostasis.

• 생명과학회지
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• 제16권6호
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• pp.1044-1051
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• 2006

The barren ground is an abnormal phenomenon of coastal ecosystem in which seaweeds, are destroyed and mostly replaced by the coralline algae containing the calcium carbonate components. To restore the seaweed forest, We have exerted an effort in the local areas, Samchuck, Korea, where barren phenomena are profound. Two methods of seaweed forest construction developed in the present study are underwater longline and seed transplantation for the brown seaweed Costaria costata, a fast growing edible seaweed. The sizes of C. costata attached on the underwater longline were $96.7{\pm}2.2mm$ of blade length and $83.6{\pm}7.7g$ of blade weight in April. Thereafter the sizes declined from May. Similar pattern was obtained from in the transplantation method with maxima of $90.4{\pm}15.8mm$ and $70.1{\pm}31.7g$ for blade length and weight, respectively in April. It appeared totality maturation from two methods in May. This maturation time is the same like that of wild C. costata.

• 한국패류학회지
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• 제25권2호
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• pp.105-112
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• 2009

키조개 양식은 근래에는 오염과 자연산 모패의 남획 등으로 점차 자원량이 감소하고 있어 이들의 양식의 필요성이 커지고 있다. 본 연구에서는 키조개의 성성숙 조절기구를 규명하기 위하여 미세조류 종류에 따른 키조개의 비만도와 생식소 및 유생의 발달단계를 비교 분석하였다. 또한 이를 바탕으로 키조개 모패로부터 인공종묘생산을 시도하였다. 키조개의 비만도를 살펴보면 어미의 연체부 지수는 사육초기에는 변화가 없었으나, 5월 중순부터 전 실험구가 먹이생물을 매우 활발히 섭취하는 경향이 있었고, 실험 종료 시에는 연체부 지수가 급격히 증가하는 경향이 있었으며, 각부 용적지수도 유사한 경향을 나타냈다. 이러한 키조개 생식소의 성숙 발달단계는 비 활성기, 초기 활성기, 후기 활성기, 완숙기, 방출 및 퇴화기의 5 단계로 구분할 수 있는데, 시험 개시일은 후기활성기로 판단되며, 시험 종료시에는 전 시험구가 완숙기와 방출기에 도달하였다. 본 연구에서는 미세조류 3 종, Chlorella ellipsoidea, Tetraselmis tetrathele, Pheasant ylum tricornutum과 키조개 성성숙의 상관관계에 대해 조사해본 결과, 키조개 암, 수 모두 Tet. 실험구에서 조직학적으로 빠른 성숙을 나타냈으며, 성성숙 유도율과 생존율 또한 Tet. 실험구에서 높은 수준을 나타냈다. 따라서 Tetraselmis tetrathele가 키조개 어미사육에서 매우 유용한 먹이생물로 판단되었다. 자연 상태의 키조개 어미로는 인공종묘생산을 기대하기는 어려운 것으로 판단되었으나, 성숙실험한 키조개 어미로부터는 5 회의 채란기간 중 반응이 있었고, D형 유생이 정상적으로 발달된 것으로 나타나 키조개는 실내 인공성숙관리에 의해 채란하는 것이 유용한 것으로 판단된다. 키조개 유생을 사육한 결과, I. galbana, Chaetoceros sp., P. lutheri 및 혼합구 전 실험구에서 실험 시작 3-5일 사이에 생존율이 매우 낮아 미세조류 종류에 따른 유의한 결과를 얻을 수 없었다. 따라서 본 연구에서는 키조개 어미 인공사육관리에 의한 인공종묘생산의 가능성을 제시하였으며, 앞으로 키조개 인공종묘 생산기술을 개발하기 위하여, 유생사육 및 치패 생산에 관한 연구가 더욱 요구된다 할 수 있다.

• KSBB Journal
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• 제24권5호
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• pp.483-488
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• 2009

본 연구는 갈조류인 다시마, 모자반, 톳을 효소적 방법으로 가수분해하여 이를 이용한 바이오 에탄올 생산 가능성을 확인하고자 하였다. 가수분해 효소 분리 및 바이오 에탄올 생산 실험 결과는 다음과 같이 요약할 수 있다. 효소는 해수 및 채취한 갈조류 시료에서 분리하였으며, 갈조류의 주요 다당류인 alginate와 laminaran에 대한 효소 활성을 측정하였다. 또한 다시마, 모자반, 톳에 대한 직접적인 갈조류 가수분해 효과를 확인 하였다. 조효소에 의한 가수분해는 alginate에서 특히 높게 나타났으며, laminaran에서도 일부 활성을 보였다. 갈조류의 전처리에서 환원당의 생성은 외분비 효소와 전체 조효소에서 크게 차이가 없었으며, 기질로는 다시마에서 최대 1.90 g/L로 가장 높게 확인되었다. 모자반과 톳에서의 가수분해가 12 h 안에 완료되는 것에 비해 다시마는 72 h 동안 반응이 지속적으로 일어났다. 에탄올 발효는 환원당 생성량과는 무관하게 나타났는데 이는 전처리 방법에 따라 갈조류 다당류의 가수분해에 미치는 영향이 다르기 때문으로 생각되며, 또한 전처리 과정에서 생성되는 부산물이 영향을 미치기 때문인 것으로 생각된다. 갈조류 에탄올 발효에서 기질로 다시마를 이용했을 때 에탄올 생산 수율이 가장 높았다. 다시마를 이용한 발효에서는 발효균주 및 효소처리에 따른 에탄올 생산량이 대략 0.90 g/L로 유사하게 나왔다. 모자반에서의 에탄올 생산은 발효균주를 Saccharomyces cerevisiae로 하였을 때 최대 0.14 g/L로 확인 되었으며, 톳에서는 Saccharomyces cerevisiae를 이용한 발효에서 에탄올생산이 전혀 확인되지 않았으며, Pachysolen tannophilus 에서만 0.09 g/L의 에탄올 생산이 생산되었다.

• 한국식품영양학회:학술대회논문집
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• 한국식품영양학회 2001년도 동계 학술심포지움
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• pp.125-125
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• 2001

Total lipid contents were 0.58∼3.00% in 9 kinds of brown algaes, 0.47∼2.16% in 5 kinds of red algaes and 0.55∼2.99% in 2 kind of green algeas, respectively. Fatty acid compositions of the algaes were determined by gas liquid chromatography. Total polyenes and n-3 polyenes of linoleic acid(18:3), stearidonic acid(18:4), eicosapentaenoic acid(20:5) and docosahexaenoic acid(22:6) were 3.88∼57.57% and 1.46∼25.67% in the brown algaes, 5.30∼39.75% and 1.17∼21.91% in the red algaes, and 7.76∼19.27% and 3.67%∼10.61% in the green algaes, respectirely. The fatty acid contents and compositions of total polyenes and n-3 polyenes were vary different in the algae groups and sepecies.