• 제목/요약/키워드: screen tests

검색결과 184건 처리시간 0.022초

Preliminary screening to assess the antimicrobial activities of extracts of evergreen woody species from South Korea against Staphylococcus aureus

  • Jang, Bo Kook;Chi, Lai Won;Cho, Ju Sung;Lee, Cheol Hee
    • Journal of Plant Biotechnology
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    • 제47권1호
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    • pp.90-99
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    • 2020
  • This study aimed to screen for plants with antimicrobial potential among the evergreen woody species of South Korea that are used for horticulture and landscaping and to provide basic information about plants with proven antimicrobial activity to underpin future research. The plant materials were extracted under various conditions, and the antimicrobial activities of the extracts were evaluated by agar diffusion assay. The screening tests demonstrated that the crude extracts of 43 species had inhibitory effects against S. aureus. The inhibitory activities of four species (Elaeocarpus sylvestris, Camellia japonica, Cleyera japonica, and Quercus salicina) were relatively higher than that of the synthetic antimicrobial agents methylparaben and phenoxyethanol. The highest inhibitory activity was observed with the leaf extracts (extracted with methanol for 30 minutes) of E. sylvestris, based on induction of the largest inhibition zone of 23.3 mm in size. In addition, solvent fractions of E. sylvestris were evaluated. The largest inhibitory zone of 23.1 mm was observed for the n-butanol fraction, which is likely to contain effective compounds that exhibit inhibitory activity against S. aureus. In contrast, n-hexane and residual aqueous fractions showed no antimicrobial activity. Overall, our findings confirm that evergreen woody plants native to South Korea have potential antimicrobial activity.

Phytochemical Screening and Antibacterial Activity Coix lacryma-jobi Oil

  • Diningrat, Diky Setya;Risfandi, Marsal;Harahap, Novita Sari;Sari, Ayu Nirmala;Kusdianti, Kusdianti;Siregar, Henny Kharina
    • Journal of Plant Biotechnology
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    • 제47권1호
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    • pp.100-106
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    • 2020
  • Coix lacryma-jobi (Hanjeli) is known to posses anti-microbial properties. Therefore, phytochemical compounds of C. lacryma-jobi have been studied to produce novel antimicrobial agents as treatments against antibiotic-resistant bacteria.The objective of this study was to determine the phytochemical composition and antibacterial activity of the C. lacryma-jobi oil against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The phytochemical composition of the oil was determined via gas chromatography mass spectrophotometry (GC-MS). Moreover, agar disk and agar well diffusion were employed to screen the antibacterial activity of the oil. An agar well diffusion test was implemented to determinate MIC's (minimum inhibitory concentrations). Dodecanoic acid, tetradecanoic acid, 2,3-dihydroxypropylester, 1,3-dioctanoin, N-methoxy-N-methyl-3,4-dihydro-2H-thiopyran6-carboxamide, propanamide, 5-Amino-1-(quinolin-8-yl)-1,2,3-triazole-4-carboxamide, and pyridine were identified in the C. lacryma-jobi oil. The MIC value of the oil was 0.031 g/L and the MBC of the oil was 0.125 g/L effective in all test bacteria. Dodecanoic acid displayed inhibitory activity against gram-positive and gram-negative bacteria. Therefore, our research demonstrated C. lacryma-jobi (Hanjeli) oil exhibited antibacterial activity against E. coli, S. aureus, and B. subtilis. These research suggest that C. lacryma-jobi root oil could be used for medicinal purposes; however clinical and in vivo tests must be performed to evaluate its potential as an antibacterial agent.

스마트폰 연동 차량의 온보드 고장진단 기능 구현과 근거리 무선통신 호환성 시험에 관한 연구 (A Study on the Implementation of the On-Board Diagnostic Function on the Smart Phone and the Compatibility Test for Short-Range Wireless Communications)

  • 구제길;양성열;송종욱;이충혁;양재수
    • 정보처리학회논문지:컴퓨터 및 통신 시스템
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    • 제5권9호
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    • pp.285-292
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    • 2016
  • 최근 차량에 블루투스와 와이파이 등 근거리 통신기능을 부가하여 스마트폰과 연동함으로써, 최신 자동차는 차량 전자제어장치인 ECU를 통하여 자동차 위치정보, 고장 진단정보 등 다양한 정보를 파악할 수 있도록 엔터테인먼트화 되어 가고 있다. 본 연구를 통하여, 온보드 진단기와 스마트폰의 근거리 통신 기능을 활용하여, 차량 온보드 진단기와 연관하여 여러 가지 스마트폰 모델과 블루투스 및 근거리 무선통신인 NFC와의 호환성 시험을 실시하고, 이를 분석해 보았다. 더 나아가, 스마트폰 연동 블루투스 및 NFC 인터페이스 기능을 갖는 온보드 진단기의 단말기 구성과 고장진단 기능 구현을 비롯하여, 고장진단용 디버깅 프로그램을 개발하였다. 또한, OBD-II 인터페이스를 통해 차량의 고장진단 데이터를 추출하였으며, 끝으로 온보드 진단기 CAN 프로토콜 구현 제시와 함께 이의 결과를 분석하였다.

In vivo와 In vitro 평가모델을 利用한 韓藥抽出物의 毛髮成長 및 促進에 미치는 實驗的 硏究 (Extracts for the Hair Growth Stimulation using In vivo and In vitro Test Models)

  • 강학천;이수형;김남권;임홍진;황충연
    • 한방안이비인후피부과학회지
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    • 제15권2호
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    • pp.53-79
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    • 2002
  • To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL/6 mouse and then hair growth promoting effect was measured using hair growth index. As a result, Prunus mume, black bean, Brassica campestris subsp. black sesame and Rubi Fructus showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Prunus mume, Eriobotryae Folium showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of the effect of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT - PCR analyses were performed. However, there were no plant extracts, which have profound effect on the gene expression of several growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another tests for inhibition of microbial such as P. acne were also carried out to find whether these plant extracts have anti -microbial activities. Rubi Fructus showed anti -microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

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The Effects of Online Home Learning in Connection with Extracurricular Activities for Lifelong Education for the Disabled at University on Cafeterias Cooking Assistance Skills of Job Search Persons with Developmental Disabilities

  • Kim, Young-Jun;Park, Jae-Kook;Kwon, Ryang-Hee
    • International Journal of Advanced Culture Technology
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    • 제9권3호
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    • pp.188-201
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    • 2021
  • The purpose of this study is to analyze the effects of online home learning in connection with extracurricular activities for lifelong education for the disabled in university on the cooking aids skills of cafeterias for the job search persons with developmental disabilities. Three people with job search developmental disabilities who have been in a state of unemployment for three years after graduating from a special high school course participated in the experiment. In order to verify the meaningful functional relationship between independent variables and dependent variables, multiple probe design across subjects, one of the main techniques of a single object study, was used. The experimental conditions according to the research design consisted of the steps of baseline, intervention, maintenance, and generalization. The dependent variable of this study is the restaurant cooking aid skills in the cafeteria, and three subskills such as side dish arrangement, sink arrangement, and dish washing were combined by task analysis. And the independent variable of this study was composed of procedures and methods to teach the environment, tools and materials related to the performance of dependent variables to the developmental disabled people at home by using real-time image technique through zoom service, and the contents of the performance by stages of task analysis. In addition, independent variables were applied to the subjects in the course of the extracurricular activities with the theme and contents of lifelong education for the disabled at university. Students who completed the above extracurricular activities practiced the intervention scene of the researcher through the screen sharing of zoom service. As a result, the subjects with developmental disabilities effectively acquired and maintained the positive response performance of dependent variables through independent variables. The subjects also showed high positive responses to generalization tests conducted in kitchens in cafeterias located elsewhere in the same university.

제주도에서 CADASIL 연구의 중요성: 역학, 진단 및 임상양상에 대한 고찰 (Importance of CADASIL research in Jeju: a review and update on epidemiology, diagnosis, and clinical spectrum)

  • 최재철;이정석;김기태
    • Journal of Medicine and Life Science
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    • 제17권3호
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    • pp.65-73
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    • 2020
  • Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a single-gene disease of the cerebral small blood vessels caused by mutations in the NOTCH3 gene on chromosome 19. Although CADASIL was known as a rare disease, recent research has suggested that the NOTCH variants could be found frequently even in the general population. The main clinical features included recurrent stroke, migraine, psychiatric symptoms, and progressive cognitive decline. On brain magnetic resonance imaging, patients with CADASIL showed multifocal white matter hyperintensity lesions, lacunar infarcts, microbleeds, and brain atrophy. Among them, lacunar infarcts and brain atrophy are important in predicting the clinical outcomes of patients with CADASIL. In the Jeju National University Hospital, we have diagnosed 213 CADASIL patients from 2004 to 2020. Most NOTCH3 mutations were located in exon 11 (94.4%), and p.Arg544Cys was the most common mutation. The mean age at diagnosis was 61.0±12.8 years. The most common presenting symptoms were ischemic stroke (24.4%), followed by cognitive impairment(15.0%), headache (8.9%), and dizziness(8.0%). Although the exact prevalence of CADASIL in Jeju is still unknown, the disease prevalence could be as high as 1% of the population considering the prevalence reported in Taiwan. Therefore, it is necessary to discover efficient biomarkers and genetic tests that can accurately screen and diagnose patients suspected of having CADASIL in this region. Ultimately, it is urgent to explore the exact pathogenesis of the disease to identify leading substances of treatment potential, and for this, multi-disciplinary research through active support from the Jeju provincial government as well as the national government is essential.

The development of a new type of functional fresh apple juice using prebiotic fibers, ginger extract, and cardamom essential oil: Antioxidant capacity and chemical analysis

  • Hamed Hassanzadeh;Mohammadyar Hosseini;Yaseen Galali;Babak Ghanbarzadeh
    • 한국식품저장유통학회지
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    • 제30권5호
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    • pp.743-757
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    • 2023
  • The formulation of a novel functional fresh apple juice enriched with dietary prebiotic fiber (inulin or polydextrose), ginger extract (GE), and cardamom essential oil (CEO) was carried out based on a combined D-optimal design. In the first stage, sensory evaluation was performed to screen and select the optimum sample for further experiments. The sensory evaluation showed that the sample containing inulin 0.25 g/100 g GE and 0.03 g/100 g CEO had the highest organoleptic score. In the second stage, various chemical experiments, including pH, acidity, formalin index, total phenol, flavonoids, antioxidant capacity, and vitamin C content, were evaluated on the selected enriched apple juices. The addition of GE and CEO caused changes in nutritional characteristics, including antioxidant capacity, total phenol, flavonoids, vitamin C, and IC50, from 35 g/100 g, 350 mg GAE/g, 17 mg/L, 370 mg/kg, and 1,800 mg/kg to 45 g/100 g, 460 mg GAE/g, 21 mg/L, 420 mg/kg, and 1,200 mg/kg respectively. The steady shear flow and dynamic oscillatory shear rheological tests were also performed on the screened samples, and results showed that the addition of dietary fiber in apple juices increased the apparent viscosity, storage modulus, loss modulus, and complex viscosity. In general, adding plant extracts and processed essential oil to apple juice increased the nutritional-nutraceutical value and sensory attributes of apple juice.

주의력결핍 과잉행동장애 한국형 치료 권고안 개정안(II) - 진단 및 평가 - (The Revised Korean Practice Parameter for the Treatment of Attention-Deficit Hyperactivity Disorder (II) - Diagnosis and Assessment -)

  • 이문수;박수빈;김경미;김현진;박상원;김윤신;이영식;권용실;신동원
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • 제28권2호
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    • pp.58-69
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    • 2017
  • Attention-deficit hyperactivity disorder (ADHD) is a highly prevalent, impairing, and comorbid disorder that persists into adulthood. ADHD should be diagnosed in the same manner as other common adult mental health disorders. The three most important components in the comprehensive evaluation of patients with ADHD are the clinical interview, medical examination, and completion and scoring of behavior rating scales. The diagnostic evaluation of ADHD should include questions about the symptoms, family history, prior evaluation and treatment of ADHD, as well as other problems including alcohol and drug use. Screening interviews or rating scales, as well as interviews, should be used. When it is feasible, clinicians may wish to supplement these components of the evaluation with the objective assessments of the ADHD symptoms, such as through psychological tests. These tests are not essential to reaching a diagnosis, however, or to treatment planning, but may yield further information about the presence and severity of cognitive impairments that could be associated with some cases of ADHD. As comorbidity is the rule rather than the exception, clinicians should carefully screen for comorbid disorders as part of a comprehensive assessment of ADHD. To receive a diagnosis of ADHD, the person must be experiencing significant distress or impairment in his or her daily functioning, and must not meet the criteria for other mental disorders which might better account for the observed symptoms, such as mental retardation, autism or other pervasive developmental disorders, mood disorders and anxiety disorders. This report aims to suggest practice guidelines for the assessment and diagnosis of children, adolescents and adults with ADHD in Korea.

Rapid detection of aneuploidy using FISH in uncultured amniocytes for prenatal diagnosis : 8-year experience

  • Hwang, Do-Yeong;Lee, Dong-Suk;Choe, Jin;Choi, Hyeh-Sook;Min, Jeong-Yong;Lee, Soo-Min;Kim, Ki-Chul
    • Journal of Genetic Medicine
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    • 제4권2호
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    • pp.190-195
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    • 2007
  • 목 적:산전진단에 있어 빠른 진단을 위해 그 유용성이 널리 알려져 있는 FISH 방법을 미배양 세포에 적용할 때, 그 민감도를 높이기 위해 본 연구소의 경험과 기준을 소개하고자 한다. 방 법:1999년 5월부터 2006년 6월까지 본연구소에서 다운증후군 고위험군, 에드워드 증후군 고위험군, 고령산모, 초음파 이상소견 등의 적응증을 주소로 시행한 7,893례의 양수검체를 대상으로 빠른 진단을 위해 8,613례의 미배양 양수세포에 FISH 검사를 시행하였다. 분석은 함춘유전연구소의 기준에 따랐으며, 기존의 세포유전학적 결과와 최종 비교하였다. 결 과:8613례의 FISH 검사 결과, 30개 이상의 세포관찰이 가능하고, 정상인 경우 정상세포의 비율이 75%, 비정상의 경우 비정상 세포의 비율이 70%에 해당하는 8,502례의 결과를 얻었으며, 세포유전학적 결과와도 일치하였다. 결 론:산전진단 시 빠른 진단을 위한 FISH검사는 매우 유용하며, 정확한 분석을 위해 그 기준을 마련하는 것은 매우 중요하다 하겠다. 그러나 비용과 인력이 많이 소요되는 한계점을 가지고 있다.

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LOCA이후 환경에서 원자로건물집수조 여과기의 수두손실에 대한 화학적 영향 (Chemical Effects on Head Loss across Containment Sump Strainer under Post-LOCA Environment)

  • 구희권;정범영;홍광;정은선;정현준;박병기;이인형;박종운
    • 한국산학기술학회논문지
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    • 제10권11호
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    • pp.3260-3268
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    • 2009
  • 냉각재상실사고이후 원전의 원자로건물집수조 여과기에서 화학적 영향을 고려한 수두손실을 종합적으로 평가하기 위한 시험장치를 개발하였다. 시험장치에서 원자로건물집수조와 시험장치에서 물 부피에 대한 여과기 면적의 비가 일치하도록 시험조건을 설정하고 시험을 수행하였다. TSP pH 조절제 조건에서 칼슘실리케이트는 시험 초기에 수두손실을 급격히 상승시켰기 때문에 원자로건물에서 모든 칼슘실리케이트를 제거하여야 함을 확인하였다. 비상노심냉각계통 살수지속시간의 차이에 따른 시험결과는 장기살수조건이 단기살수조건에 비해 12배 정도 높은 수두손실을 보였다. 살수조건 시험결과를 화학적 영향이 없는 수두손실과 비교하면 단기살수와 장기살수의 각 조건에서 5.6배 및 60.8배 수두손실이 증가하는 결과를 보였다. 화학적 영향은 재순환수에 노출된 물질의 양에 따라 초기의 일정기간 동안 알루미늄 및 아연도금 판의 부식에 의해 급격히 증가하고 이들이 부동피막을 형성한 이후에는 NUKONTM 및 콘크리트 등에서 침출된 화학종의 침전에 기인하여 증가율이 감소하는 경향을 보였다. 실험결과는 TSP에 의한 알루미늄의 부동피막 형성이 살수시간이 길어지고 알루미늄의 양이 많을 경우 효과적이지 않다는 것을 보였다.