• Korean Journal of Plant Resources
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• v.4 no.1
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• pp.5-12
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• 1991

A combination of acerocarininc-Wright C-banding technique was utilized to identify each chromosomes in durum wheat ,Triticum durum var. Hordeiforme (2n=4x=28 AABB), This technique elucidated qualitativr and quantitative traits of the indi-vidual chromosomes In coinplement. Most comspicuous bands were observed at thecentromere of B-genome chronmosomes. Each chromosomes of A-genome had some-what weak centromeric, proximal and terminal bands. Chromosomes 2A and 4A hasa small subterminal bands. 6A is smallest and metacentric chromosome and , has two faint interstitial band. Chromosomes 1B and 6B showed satellite and constriction lage band. Short arm of 3B has three heavily interstitial bands. Both arms of chromosome 4B has a lagc centromeric band and a very lage proximal band. 5B had heavilycentromeric band and the long arm showed prominent two interstitial bands. Chromo-somes 25 and 7B has a small terminal band of both arms.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.123-125
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• 2005

Many researchers conducted the effort for improving the classification accuracy of satellite image. Most of the study has used optical spectrum information of each pixel for image classification. By applying this method for high resolution satellite image, number of class becomes increase. This situation is remarkable for house, because the roof of house has variety of many colors. Even if the classification is carried out for many classes, roof color information of each house is not necessary. Most of the case, we need the information that object is house or not. In this study, we propose the method for detecting the object by using Genetic Algorithms (GA). Aircraft was selected as object. It is easy for this object to detect in the airport. An aircraft was taken as a template. Object image was taken from QuickBird. Target image includes an aircraft and Haneda Airport. Chromosome has four or five parameters which are composed of number of template, position (x,y), rotation angle, rate of enlarge. Good results were obtained in the experiment.

• Journal of Genetic Medicine
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• v.8 no.2
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• pp.135-138
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• 2011

The authors of the present study report the prenatal detection of a chromosomal abnormality with additional satellites on the distal short arm of chromosome 8. A 35-year-old woman was referred for amniocentesis because of her advanced maternal age and positive result for maternal serum screening test. Cytogenetic analysis of cultured amniocytes showed a satellite 8p chromosome. The satellite 8p chromosome was positive for nucleolus organizer region (NOR) staining. The parents' karyotypes were normal. Fluorescence in situ hybridization (FISH) study for metaphases of fetal amniocytes revealed a cryptic translocation of chromosomes 8p and 22p. The fetal karyotype was described as 46,XY,8ps.ish t(8;22)(p23.3;p11.2) (D8S504-;D8S504+)dn. The parents decided to continue the pregnancy and a phenotypically normal boy was born at 38 weeks of gestation. In case of de novo terminal NORs detected prenatally, more accurate cytogenetic and molecular analysis should be performed in order to rule out cryptic chromosomal rearrangement among other chromosomes.

• Korean Journal of Head & Neck Oncology
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• v.16 no.1
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• pp.3-8
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• 2000

Background and Objectives: Adenoid cystic carcinoma of salivary glands is characterized by insidious growth over many years, local recurrences, and distant metastasis and classified to three distinct histologic subtypes: tubular, cribriform, and solid. The solid type is known to have the worst prognosis. However, histopathologic heterogeneity is observed in tumors from the same patient. We have attempted to elucidate the genotypic differences, characterized by polysomies of chromosome 17, in adenoid cystic carcinoma according to the phenotypic histopathologic heterogeneity. Materials and Methods: Fluorescence in situ hybridization was performed on formalin-fixed paraffin blocks from seven patients with head and neck adenoid cystic carcinoma, using the centromeric $\alpha$-satellite probe of chromosome 17 to detect nuclei exhibiting polysomy. The difference in polysomeric chromosome expression in cribriform, tubular, solid type and type I, II, III according to the Szanto classification was analyzed. Results: Polysomy of chromosome 17 was found in 15.28% of the cribriform type, in 15.68% of the tubular type, and in 18.87% of the solid type. The proportion of polysomy was statistically higher in the solid type than in the cribriform type(p<0.05), and the proportion of polysomy increased progressively from type 1 to type 3, but this trend was statistically insignificant(p>0.05). Conclusion: We suggest that there may be genetic variations in tumor from the same patient depending on the histopathologic heterogenetiy in adenoid cystic carcinomas.

• Journal of Genetic Medicine
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• v.1 no.1
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• pp.11-16
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• 1997

A nine month old male child presenting degenerating right ulna (massive osteolysis) has been followed up for two years. The bone completely disappeared due to abscesses on the right forearm and without orthopedic or haematological complications. Repeated lymphocyte cultures showed somatic pairing (mostly chromosome pair 5), end to end association involving chromosome 14, 21, 21 and 16, and satellite enlargement in a high proportion of cells with an otherwise normal 46,XY karyotype. These observations are compared with 13 other types of orthopaedic patients, and we opine that cumulative picture of chromosomal aberrations appears to correspond with the present rare anomaly "Mono Ostolic Osteolysis" involving right ulna. None of the controls or any other orthopaedic anomaly studied hereunder exhibits this chromosomal picture.

• Korean Journal of Ichthyology
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• v.7 no.1
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• pp.79-83
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• 1995

Karyotypic analysis was performed for Labridae fishes, Pseudolabrus japonicus, Halicho eres tenuispinis, H. poecilopterus and Pteragogus flagellifera from coastal area of Cheju Island in Korea. The chromosome numbers(Karyotypes) were 42(4M+24SM+14ST, A), 48(2M+2SM+44ST, A), 48(2M+46ST, A) and 42(4M+24SM+14ST, A) in P. japonicus, H. tenuispinis, H. poecilopterus and P. flagellifera, respectively. Heteromorphic sex chromosome was not found in both sexes of each Labridae fishes. However, large satellites were located on the largest subtelocentrics in P. japonicus and P. flagellifera.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.369-375
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• 1997

Studies were conducted to determine the efficiency of decondensation protocols. Sperm obtained from seven normal donors was immediately washed after liquefaction and then decondensed using the method of West et al. (1989) and my original protocol. My optimized protocol entailed mixing 1 ml aliquots of semen with 4 ml phosphate buffered saline (PBS). Following centrifugation, pellets were resuspended in 1 ml PBS containing 6 mM EDTA. After centrifugation, pellets were resuspended in 1 ml PBS containing 2 mM dithiothreitol at $37^{\circ}C$ for 45 min. Following mixing with 2 ml PBS and centrifugation, pellets were resuspended by vortexing. While vortexing, 5 ml of fixative were gently added. Slide preparation was accomplished using the smear method and it was stored at $4^{\circ}C$. When comparing these protocols, the degree of sperm decondensation and head swelling was monitored by measuring nuclear length, area, perimeter, and degree of roundness using FISH analysis software. Apparent copy number for chromosome 1 and, separately, for the sex chromosomes was determined by FISH using satellite DNA probes for loci DIZ1, DXZ1 and DYZ3. Sperm treated by my decondensation protocol showed significant increases (p<0.05) in length, area, perimeter, and degree of roundness. There was a significant decrease (p<0.05) in the frequency of nuclei displaying no signal but no change in the frequency of nuclei with two signals in samples decondensed by my protocol. My data suggested that decondensation using my original protocol may lower the frequency of cells with spurious "nullisomy" due to hybridization failure without inducing spurious "disomy" resulting from increased distances between split signals.

• Clinical and Experimental Reproductive Medicine
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• v.16 no.1
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• pp.41-55
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• 1989

Lymphocyte chromosome preparations obtained by the micromethod (Arakaki and Sparkes, 1963) from 234 our patients (165 females and 69 males) were analysed by C-, NOR-and GC-bandings for chromosome heteromorphisms. The centromeric regions of chromosomes 1,9,16 and the long arm of the Y chromosomes were tested for C heteromorphism. Minor variations found in this study such as inv(9), prominant short arms and large satellites of acrocentrics were also examined by appropriate banding techniques. Of the 234 probands, a total of 125 different C-variants were detected, and the average frequency of the variants per individual was estimated to be 0.53. The observed variations were as follows : 99 qh variants, 5 pericentric inversions of chromosome 9, and 21 satellite and/or short arm variants.

• Journal of Life Science
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• v.15 no.1 s.68
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• pp.45-48
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• 2005

The aim of this study was to construct a correct pedigree of miniature horses (MH). The sex of MH was detected by PCR amplification of the sex determining region of the Y chromosome gene (SRY) prior to parentage testing. Ten random MH samples for parentage testing were genotyped by using 16 micro satellite markers. Since the SRY band (430 bp) was detected in horses No.1, 2, 6, 7, 8, 9, 10, these are male. However, the DNA segment was not identified in horses No.3, 4, and 5, which therefore are female. After genotyping, parentage testing was performed according to Mendelian fashion and International Society for Animal Genetics (ISAG) guideline. Of the 10 MH, 3 were qualified by the compatibility of 16 markers according to Mendelian fashion in the present DNA typing for parentage verification. These results can provide basic information for developing parentage verification and an individual identification system in MH.

• Molecules and Cells
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• v.26 no.3
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• pp.319-322
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• 2008

Little is known about the chromosomal variability and polymorphism existing in mitotic chromosomes of Citrus, mainly due to lack of reliable chromosomal markers and small chromosome size. To test the hypothesis of chromosomal polymorphism and provide the foundation of the genome organization in the Citrus cultivars, we have developed molecular cytogenetic markers for 13 Citrus species collected from Jeju island, Korea. In this study, we demonstrated that the chromosomal locations of cytogenetic markers are quite variable and extremely polymorphic, in contrast to the previous studies. The data obtained in this study will be of utmost importance in cytological systematics and karyotyping of the Citrus species.