Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Extensive Chromosomal Polymorphism Revealed by Ribosomal DNA and Satellite DNA Loci in 13 Citrus Species

  • Kang, Sung-Ku (Department of Fruit Tree Science, Rural Development Administraion, Korea National Agricultural College) ;
  • Lee, Dong-Hoon (Citrus Experiment Station, National Institute of Subtropical Agriculture Rural Development Administration) ;
  • An, Hyun-Ju (Citrus Experiment Station, National Institute of Subtropical Agriculture Rural Development Administration) ;
  • Park, Jae-Ho (Citrus Experiment Station, National Institute of Subtropical Agriculture Rural Development Administration) ;
  • Yun, Su-Hyun (Citrus Experiment Station, National Institute of Subtropical Agriculture Rural Development Administration) ;
  • Moon, Young-eel (Citrus Experiment Station, National Institute of Subtropical Agriculture Rural Development Administration) ;
  • Bang, Jae-Wook (School of Bioscience and Biotechnology, Chungnam National University) ;
  • Hur, Yoonkang (School of Bioscience and Biotechnology, Chungnam National University) ;
  • Koo, Dal-Hoe (School of Bioscience and Biotechnology, Chungnam National University)
  • Received : 2008.01.11
  • Accepted : 2008.04.08
  • Published : 2008.09.30
⟨ Previous Next ⟩

Abstract

Little is known about the chromosomal variability and polymorphism existing in mitotic chromosomes of Citrus, mainly due to lack of reliable chromosomal markers and small chromosome size. To test the hypothesis of chromosomal polymorphism and provide the foundation of the genome organization in the Citrus cultivars, we have developed molecular cytogenetic markers for 13 Citrus species collected from Jeju island, Korea. In this study, we demonstrated that the chromosomal locations of cytogenetic markers are quite variable and extremely polymorphic, in contrast to the previous studies. The data obtained in this study will be of utmost importance in cytological systematics and karyotyping of the Citrus species.

Keywords

Acknowledgement

Supported by : Rural Development Administration

References

  1. Agarwal, P.K. (1987). Karyotype of Citrus tamurana (Tan.). Chromosome Inf. Serv. 42, 3-5
  2. Befu, M., Kitajima, A., and Hasegawa, K. (2001). Chromosome composition of some Citrus species and cultivars based on the chromomycin A3 (CMA). J. Jpn. Soc. Hort. Sci. 70, 83-88 https://doi.org/10.2503/jjshs.70.83
  3. Brasileiro-Vidal, A.C., Santos-Serejo, J., Soares Filho, W.S., and Guerra, M. (2007). A simple chromosomal marker can reliably distinguishes mçåÅáêìë from Citrus species. Genetica 129, 273-279 https://doi.org/10.1007/s10709-006-0007-4
  4. Carvalho, R., Soares Filho, W.S., Brasileiro-Vidal, A.C., and Guerra, M. (2005). The relationships among lemons, limes and citron: a chromosomal comparison. Cytogenetic Genome Res. 109, 276-282 https://doi.org/10.1159/000082410
  5. de Moraes, A.P., Soares Filho, W.S., and Guerra, M. (2007). Karyotype diversity and the origin of grapefruit. Chromosome Res. 15, 115-121 https://doi.org/10.1007/s10577-006-1101-2
  6. Do, G.S., Seo, B.B., Yamamoto, M., Suzuki, G., and Mukai, Y. (2001). Identification and chromosomal location of tandemly repeated DNA sequences in Allium cepa. Genes Genet. Syst. 76, 53-60 https://doi.org/10.1266/ggs.76.53
  7. Esen, A., and Soost, R.K. (1972). Aneuploidy in Citrus. Am. J. Botany 59, 473-477 https://doi.org/10.2307/2441528
  8. Fann, J.Y., Kovarik, A., Hemleben, V., Tsirekidze, N.I., and Beridze, T.G. (2001). Molecular and structural evolution of Citrus satellite DNA. Theor. Appl. Genet. 103, 1068-1073 https://doi.org/10.1007/s001220100719
  9. Frost, H.B. (1925). Tetraploidy in Citrus. Proc. Natl. Acad. Sci. USA 11, 535-537
  10. Gerlach, O.L., and Bedrock, J.R. (1979). Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res. 7, 1869-1885 https://doi.org/10.1093/nar/7.7.1869
  11. Guerra, M. (1987). Cytogenetics of rutaceae IV. Structure and systematic significance of interphase nuclei. Cytologia 52, 213-222 https://doi.org/10.1508/cytologia.52.213
  12. Guerra, M. (1993). Cytogenetics of rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining. Heredity 71, 234-241 https://doi.org/10.1038/hdy.1993.131
  13. Guerra, M., dos Santos, K.G.B., Silva, A.E.B.E., and Ehrendorfer, F. (2000). Heterchomain banding patterns in Rutaceae-Aurantioideae a case of parallel chromosomal evolution. Am. J. Botany 87, 735-747 https://doi.org/10.2307/2656860
  14. Hirai, M., and Kajiura, I. (1987). Genetic analysis of leaf isozymes in Citrus. Jpn. J. Breed. 37, 377-388 https://doi.org/10.1270/jsbbs1951.37.377
  15. Hizume, M., Shibata, F., Matsusaki, Y., and Garajova, Z. (2002). Chromosome identification and comparative karyotypic analyses of four máåìë species. Theor. Appl. Genet. 105, 491-497 https://doi.org/10.1007/s00122-002-0975-4
  16. Jiang, J., and Gill, B.S. (2006). Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research. Genome 49, 1057-1068 https://doi.org/10.1139/G06-076
  17. Kato, A., Lamb, J.C., and Birchler, J.A. (2004). Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc. Natl. Acad. Sci. USA 101, 13554-13559
  18. Koo, D.H., Jin, D.C., Hur, Y., and Bang, J.W. (2002). Karyotype analysis of a Korean cucumber cultivar Cucumis sativus L. cv. Winter Long) using C-banding and bicolor fluorescence in situ hybridization. Mol. Cells 13, 413-418
  19. Koo, D.H., Plaha, P., Lim, Y.P., Hur, Y., and Bang, J.W. (2004). A high resolution karyotype of Brassica rapa spp. pekinensis revealed by pachytene analysis and multicolor fluorescence in situ hybridization. Theor. Appl. Genet. 109, 1346-1352 https://doi.org/10.1007/s00122-004-1771-0
  20. Koo, D.H., Choi, H.W., Cho, J., Hur, Y., and Bang, J.W. (2005). A high-resolution karyotype of cucumber Cucumis sativus L . "Winter Long") revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization. Genome 48, 534-540 https://doi.org/10.1139/g04-128
  21. Krug, C.A., and Bacchi, O. (1943). Triploid varieties of Citrus . J. Hered. 34, 277-283 https://doi.org/10.1093/oxfordjournals.jhered.a105303
  22. Lengerova, M., Kejnovsky, E., Hobza, R., Macas, J., Grant, S.R., and Vyskot, B. (2004). Multicolor FISH mapping of the dioecious model plant, Silene latifolia. Theor. Appl. Genet. 108, 1193-1199 https://doi.org/10.1007/s00122-003-1568-6
  23. Lim, K.H., de Jong, H., Yang, T.J., Park, J.Y., Kwon, S.J., Kim, J.S., Lim, M.H., Kim, J.A., Jin, M., Jin, Y.M., et al. (2005). Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica rapa. Mol. Cells 19, 436-444
  24. Miranda, M., Ikeda, F., Endo, T., Moriguchi, T., and Omura, M. (1997). Chromosome markers and alterations in mitotic cells from inter-specific Citrus somatic hybrids analysed by fluorochrome staining. Plant Cell Rep. 16, 807-812 https://doi.org/10.1007/s002990050325
  25. Mukai, Y., Nakahara, Y., and Yamamoto, M. (1993). Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36, 489-494 https://doi.org/10.1139/g93-067
  26. Naithani, S.P., and Raghuvanshi, S.S. (1958). Cytogenetical studies in the genus Citrus . Nature 181, 1406-1407
  27. Roose, M.L., Schwarzacher, T., and Heslop-Harrison, J.S. (1998). The chromosomes of Citrus and Poncirus species and hybrids: Identification of characteristic chromosomes and physical mapping of rDNA loci using in situhybridization and fluorochrome banding. J. Hered. 89, 83-86 https://doi.org/10.1093/jhered/89.1.83
  28. Sadder, T., and Weber, G. (2001). Karyotype of maize (Zea maus L.) mitotic metaphase chromosomes as revealed by fluorescence in situ hybridization (FISH) with cytogenetic DNA markers. Plant Mol. Biol. Rep. 19, 117-123 https://doi.org/10.1007/BF02772153
  29. Yamamoto, M., Abkenar, A.A., Matsumoto, R., Nesumi, H., Yoshida, T., Kuniga, T., Kubo, T., and Tominaga, S. (2007). CMA banding patterns of chromosomes in major Citrus species. J. Jpn. Soc. Hort. Sci. 76, 36-40 https://doi.org/10.2503/jjshs.76.36
  30. Yamamoto, M., and Tominaga, S. (2003). High chromosomal variability of mandarins (Citrus spp.) revealed by CMA banding. Euphytica 129, 267-274 https://doi.org/10.1023/A:1022284908424
  31. Yamamoto, M., and Tominaga, S. (2004). CMA banding pattern of chromosomes is useful for the identification of chromosome doubling in haploid Citrus. Breed. Sci. 54, 351-354 https://doi.org/10.1270/jsbbs.54.351