• Proceedings of the Korean Association for Survey Research Conference
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• 2005.12a
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• pp.143-157
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• 2005

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1855-1862
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• 2016

Cronobacter sakazakii (C. sakazakii) is a foodborne pathogen, posing a high risk of disease to infants and immunocompromised individuals. In order to develop a quick, easy, and sensitive assay for detecting C. sakazakii, a rabbit anti-C. sakazakii immunoglobulin G (IgG) was developed using sonicated cell protein from C. sakazakii. The developed anti-C. sakazakii (IgG) was of good quality and purity, as well as species-specific. The developed rabbit anti-C. sakazakii IgG was attached to the surface of a sulforhodamine B-encapsulated liposome to form an immunoliposome. A test strip was then prepared by coating goat anti-rabbit IgG onto the control line and rabbit anti-C. sakazakii IgG onto the test line, respectively, of a plastic-backed nitrocellulose membrane. A purple color signal both on the test line and the control line indicated the presence of C. sakazakii in the sample, whereas purple color only on the control line indicated the absence of C. sakazakii in the sample. This immunochromatographic strip assay could produce results in 15 min with a limit of detection of $10^7CFU/ml$ in C. sakazakii culture. The immunochromatographic strip assay also showed very good specificity without cross-reactivity with other tested Cronobacter species. Based on these results, the developed immunochromatographic strip assay is efficient for the detection of C. sakazakii and has high potential for on-site detection.

• The Korean Journal of Food And Nutrition
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• v.12 no.1
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• pp.91-94
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• 1999

The amount of creatinine contained in the beef imported beef pork and chicken marketing four beef pork, and chicken, marketing four beef stocks seven ramyon soups and three hams and sausages were studied by spectrophotometry with alka-line picrate. Content of creatinine in beef was 252mg/100g of sample and it is higher than that of impor-ted beef. The amount of creatinine increases as follows; chicken

• Proceedings of the KSRS Conference
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• 2004.10a
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• pp.344-346
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• 2004

IKONOS 1m satellite imagery is particularly well suited for 3-D feature extraction and 1 :5,000 scale topographic mapping. Because the image line and sample calculated by given RPCs have the error of more than 11m, in order to be able to perform feature extraction and topographic mapping, rational polynomial coefficients(RPCs) camera model that are derived from the very complex IKONOS sensor model to describe the object-image geometry must be refined by several Ground Control Points(GCPs). This paper presents a quantitative evaluation of the geometric accuracy that can be achieved with IKONOS imagery by refining the offset and scaling factors of RPCs using several GCPs. If only two GCPs are available, the offsets and scale factors of image line and sample are updated. If we have more than three GCPs, four parameters of the offsets and scale factors of image line and sample are refined first, and then six parameters of the offsets and scale factors of latitude, longitude and height are updated. The stereo images acquired by IKONOS satellite are tested using six ground points. First, the RPCs model was refined using 2 GCPs and 4 check points acquired by GPS. The results from IKONOS stereo images are reported and these show that the RMSE of check point acquired from left images and right are 1.021m and 1.447m. And then we update the RPCs model using 4 GCPs and 2 check points. The RMSE of geometric accuracy is 0.621 m in left image and 0.816m in right image.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.901-905
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• 2005

Monte Carlo localization is known to be one of the most reliable methods for pose estimation of a mobile robot. Much research has been done to improve performance of MCL so far. Although MCL is capable of estimating the robot pose even for a completely unknown initial pose in the known environment, it takes considerable time to give an initial estimate because the number of random samples is usually very large especially for a large-scale environment. For practical implementation of the MCL, therefore, a reduction in sample size is desirable. This paper presents a novel approach to reducing the number of samples used in the particle filter for efficient implementation of MCL. To this end, the topological information generated off- line using a thinning method, which is commonly used in image processing, is employed. The topological map is first created from the given grid map for the environment. The robot scans the local environment using a laser rangefinder and generates a local topological map. The robot then navigates only on this local topological edge, which is likely to be the same as the one obtained off- line from the given grid map. Random samples are drawn near the off-line topological edge instead of being taken with uniform distribution, since the robot traverses along the edge. In this way, the sample size required for MCL can be drastically reduced, thus leading to reduced initial operation time. Experimental results using the proposed method show that the number of samples can be reduced considerably, and the time required for robot pose estimation can also be substantially decreased.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.2 s.33
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• pp.47-67
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• 2007

Objective : As a result of increasing amount of ultraviolet ray, skin problems including sunburn, rapid skin aging, melanoma, and even skin cancer continue to rise. In the present study, the effect of oriental herbal extract, Daehwanggo(大黃膏,DH) and Daehwanggogasnagbakpi(大黃膏加桑白皮,DS), as external application, on the skin damage, was investigated. Methods : 30 mice were equally distributed into 3 groups : control, UVB-control and UVB-irradiated and DS-treated group. Also mouse melanoma cell lines were cultured. Tyrosinase inhibition was measured to analyze the UN-protection effect. Melanogenesis in the UV-irradiated melanoma cell lines was compared in DS-treated cell line and control cell line. Sample skin from the ear tissue of the 3 groups were analyzed to observe the inflammatory response, T cell differentiation, apoptosis of keratinocytes. Results : The tyrosinase was more significantly inhibited in the DS group compared to DH group. Antioxidative effects was more prominent in DS group when superoxide dismutase was measured. Both the DS- and DH-treated cell lines showed significantly reduced melanogenesis. The reduction of external skin damage including erythematous papule, eczema, keratinocyte, pyopoiesis was observed in the DS- and DH-treated sample cells. In terms of the effect on the skin damage, sunburn cell, activated skin mast cells, secretion of IL-12, manifestation of HSP70, hyperplasia of epithelial cells, MMP-9 and destruction of the collagen were all significantly improved in the DS-treated sample cells. Melanin cells and the apoptosis in the melanoma cell line were decreased. Conclusion : DH and DS were traditionally applied externally for the scald in the oriental medicine. The present study elucidated the possibility of herbal extracts to be used as ultraviolet protectives. Further investigations are needed to assure the clinical application.

• Geotechnical Engineering
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• v.12 no.5
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• pp.17-26
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• 1996

The undrained behavior of Asan marine soil was investigated by using an automated triaxial testing device. The stress-strain behavior at the preand postfailure state of marine soil under undrained compression and eatension conditions was compared with the behavior of pure silt, pure clay and the overall behavior of Asan marine soil was predicted with the modified Camflay model and the bounding surface model. The marine soil sampled in Asan bay area was clayey silts with 70oA silt-30% clay content and the testing samples were prepared in both undisturbed and remolded conditions. All samples are normally consolidated with 400 kPa of effective mean confining pressure and each sample is unloaded to 200, 100, 67 kPa, respectively. And then the shear test was performed with different confining pressure. According to experimental results, there exists an unique failure line whose slope is lower than silt's and higher than clay's. It is identified that the undrained shear strength of normally consolidated samples increases after crossing the phase transformation line because of volume dilation tendency which is not seen in clay. Overconsolidated samples show different soil behavior compared with pure silt due to its tendency of change in volume. It is also found that the overall behavior of Asan marine soil cannot be predicted precisely with the modified Cam-clay model and the bounding surface model.

• 제어로봇시스템학회:학술대회논문집
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• 1992.10a
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• pp.567-571
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• 1992

A guidance loop of the missile system which uses a command to line of sight(CLOS) guidance law is designed based on LQG theory. In the environment of the severe tracking system noise, the system requires small aerodynamic control fin travel and small miss distance simultaneously. Results from a sample airframe shows good performance against a randomly maneuvering target.

• Proceedings of the IEEK Conference
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• 2003.07a
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• pp.310-313
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• 2003

Complex permittivities of human head simulating liquids were measured by a sample holder of terminated slotted coaxial line with a movable probe at mobile communication frequencies. The validity of the liquid measurement system was checked by experiments with the reference liquids. Liquids of ingredients for human brain suggested in IEEE draft and those made by the authors were measured by this slotted line system.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.32B no.9
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• pp.1231-1238
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• 1995

In on-line character recognition, to segment input character is important. This paper proposes an internal character segmentation algorithm. The internal segmentation algorithm produces candidate words by considering possible combinations of Korean alphabets. In this process, we make use of projections of strokes onto the horizontal axis to remove ambiguities among candidate words. As a result of experiments, the internal segmentation algorithm shows better performance than external segmentation algorithm as the gap between sample characters becomes smaller.