• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.890-894
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• 2001

The isolation of genomic DNA from mammalian cells is usually performed by cell lysis followed by protein digestion, extraction, and finally, ethanol precipitation of the chromosomal DNA. However, in the case of large sample numbers or when only small amounts of starting materials are available, such conventional methods are not efficient and are cumbersome to be applied. Some alternative methods have been described as well as having commercial DNA isolation kits to be available, nevertheless, there is room left for much improvement. In the present study, a novel method is introduced, where it simplifies conventional protocols by omitting some time-consuming steps such as protease incubation or DNA precipitation and its resuspension. Using paramagnetic silica resins, the genomic DNA was purified over a magnetic field, and the bound DNA was eluted with a low-salt buffer. The fidelity and effectiveness of this novel method was determined by using normal and apoptotic cells as a starting material and then compared to other protocols. The high speed and convenience along with its high efficiency in detecting apoptotic chromosomal DNA will prove this method to be an improved alternative in the isolation of genomic DNA from mammalian cells.

• 한국신재생에너지학회:학술대회논문집
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• 2008.10a
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• pp.203-207
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• 2008

Prestack depth migration is used to image for complex geological structure such as faults, folds, and subsalt. In this case, it is widely used the surface reflection data as a input data. However, the surface reflection data have intrinsic problems to image the subsalt and the salt flank due to the complex wavefields and multiples which come from overburden. For overcoming the structural defect of the surface reflection data in the imaging, I used the virtual sources in terms of seismic interferometry to image the subsurface and suppress the multiples using the velocity model of the lower part of the virtual sources. The results of the prestack depth migration using virtual source gathers and velocity model below receivers are similar geological interfaces to the results from shot gathers of the conventional ocean bottom seismic survey. And especially artificial interfaces by multiples were suppressed without applying any other data processing to eliminate multiples. This study results by numerical modeling can make a valuable imaging tool when it is applied to satisfied field data for specific condition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.4
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• pp.267-274
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• 1996

This experiment was carried out to investigate the effect of pasture types(mixture and mono tall fescue pasture) and endophyte infection on the animal behavior of Korean native cattle at the Experimental Field of Grassland and Forage Crops Division, National Livestock Research Institute, Suweon, in 1994. The results obtained are sumunarized as follows: DM intake of Korean native cattle was increased with mixture and endophyte-free tall fescue for both pasture types. Mixture and endophyte-free tall fescue showed good animal preference. Daily grazing time was increased with mixture and endophyte-free tall fescue in monoculture, while annual grazing period was increased with monoculture and endophyte-infect tall fescue. Amount of drinking water of Korean native cattle was increased with tall fescue monoculture rather than mixture, however the amount of drinking water was higher with endophyte-infect tall fescue in monoculture. Salt intake of Korean native cattle was increased with mixture and endophyte-6ee tall fescue in monoculture. Rectal temperature of Korean native cattle was not influenced by pasture types and endophyte infection. Based on the results of this experiment, DM intake of Korean native cattle and daily grazing time were increased by mixture and endophyte-free tall fescue with good quality.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.3
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• pp.252-258
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• 2013

The quality and quantity of food organisms in fish seed production are important. The marine microalgae Nannochloropsis oculata are used as initial food organisms in the field. We investigated the effects of salinity (0, 10, 20, 30, 40 and 50 psu) on the lipid and fatty acid composition of N. oculata. Cultivation of N. oculata at varying salinities showed the highest growth rate at 20 psu. Total lipid content ranged from 17.26 to 18.63% at salinities from 0 to 50 psu). The nonpolar lipid content increased markedly at 30 psu and was highest at 15.55%. The polar lipid content was lowest at 30 psu, by 84.45%. It was also found that the omega-3 and EPA contents were inversely proportional to salt concentration. For the polar and nonpolar lipid compositions, there was no significant effect of salinity. Omega-3 polyunsaturated fatty acid content especially the content of EPA in the seawater larvae is the essential fatty acid in this food organism. It is thus advantageous to culture N. oculata at 20 psu.

• Magazine of the Korean Society of Agricultural Engineers
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• v.42 no.3
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• pp.66-75
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• 2000

Effect of reclaimed sewage on the paddy rice culture was examined by field experiment for two consecutive years. The domestic sewage was treated by the constructed wetland and the effluent of the treatment wetland was used for irrigation water. The reclaimed sewage was diluted before irrigation in the first year and it was used without dilution in the second year experiment. Growth components and yields were compared against the control plot where conventional method was applied. And also soil characteristics of the plots before and after reclaimed sewage irrigation were analyzed Generally addition of the reclaimed sewage irrigation didn't affect paddy rice culture adversely and even enhancement was observed. Fertilization was thought to be important factor for rice culture rather than irrigation water quality. Conventional fertilization and reclaimed sewage irrigation which contained high nutrient concentration resulted in better growth and more yield. Unlike widespread concern lodging did not happen even in the case of irrigation with average 90mg/L of T-N and conventional fertilization. Soil characteristics changed after irrigation and significant EC increasing was observed for the reclaimed sewage irrigation plots. From soil analyses salt accumulation could be a more potential problem than nutrients like nitrogen and phosphorus in the use of reclaimed sewage irrigation. Overall reclaimed sewage irrigation was thought to be one of practical alternatives for the ultimate disposal of sewage in rural area.

• Magazine of the Korean Society of Agricultural Engineers
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• v.33 no.2
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• pp.104-111
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• 1991

In Korea, small-scale reclaimed areas have been suffering from many problems because of the lack of comprehensive developing strategy although considerable investments have been inputed by the public sector since 1970's. For 3 reclaimed sites in Chonnam Province choser as case study areas, the analysis, the second attempt of widely-spanned studies on areal conditions, concentrated on their farming and economic conditions. Its results were as follows ; 1. Although farming pattern has been transferred to the full4ime rice cropping type by the creation of reclaimed paddy field, farming size in the areas has not been increased more than that in existing agricultural areas. This means that agricultural planning should be included in the initial stage of reclamation projects, especially with reference to the substantial enlargement of farming size. 2. Block parcelling of severely fragmented holdings in new and old lands should be carried out, which can make farming activities efficient and farming route shortened. In large-scale reclaimed areas, new village planning could be considered in its central zone for efficent farming. 3. Because soil in the areas contains much more salt and water than that in other areas, new design methodology should be introduced for the efficient use of agricultural machines in reclaimed areas. 4. There are deep-seated economic problems in reclaimed area, which have been caused by very poor level and agriculturally dominated structure of household income. These problems should motivate farmers to give up positive action for qualitative and quantitative improvement in farming.

• Journal of the Korean Ceramic Society
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• v.56 no.5
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• pp.474-482
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• 2019

Chromium-doped zinc ferrite nanoparticles with the general formula Cr_yZnFe_2-yO₄ (y = 0, 0.025, 0.05, 0.075, and 0.1) were synthesized by a surfactant-assisted chemical co-precipitation route using metal nitrate salt precursors. The phase purity and structural parameters were determined by powder X-ray diffraction. The concentration of Cr³⁺ doped into ZnFe₂O₄ (ZF) noticeably affected the crystallite size, which was in the range of 22 nm to 36 nm, and all samples showed a single cubic spinel structure without any secondary phase or impurities. The lattice parameter, X-ray density, and skeletal density increased with an increase in the Cr-doping concentration; on the other hand, a decreasing trend was observed for the particle size and porosity. The influence of Cr³⁺ substitution on ZF magnetic properties were studied under an applied field of 15 kOe. The overall results revealed that the incorporation of a small amount of Cr dopant changed the structural, electrical, and magnetic properties of ZF.

• Bulletin of the Korean Chemical Society
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• v.33 no.6
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• pp.1949-1954
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• 2012

We have developed a novel and eco-friendly synthetic route for the preparation of a two-dimensional layered zinc hydroxide with intercalated nitrate anions. The layered zinc hydroxide nitrate, called 'zinc basic salt', was, in general, successfully synthesized, using an electron beam irradiation technique. The 2-propanol solutions containing hydrated zinc nitrate were directly irradiated with an electron-beam at room temperature, under atmospheric conditions, without stabilizers or base molecules. Under electron beam irradiation, the reactive OH radicals were generated by radiolysis of water molecules in precursor metal salts. After further radiolytic processes, the hydroxyl anions might be formed by the reaction of solvated electrons and the OH radical. Finally, the $Zn_5(OH)_8(NO_3)_2{\cdot}2H_2O$ was precipitated by the reaction of zinc cation and hydroxyl anions. Structure and morphology of obtained compounds were characterized by powder X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and high resolution transmission electron microscopy (HR-TEM). The chemical components of the products were determined by Fourier transform infrared spectroscopy (FT-IR) and elemental analysis (EA). The thermal behavior of products was studied by thermogravimetric (TG) and differential thermal analysis (DTA).

• Journal of the Korean Institute of Landscape Architecture
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• v.26 no.3
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• pp.278-287
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• 1998

The halophytes are considered less important in our field, as the land reclamation executed continuously by public or individual. So this research is to use plants more active on the reclaimed land and to verify their utility in the aspect of the landscape planning, design and complementaton. This research is performed at the construction site of Incheon International Airport in Youngjong-do located in West-sea of Korea. Following proposals are the major conclusions drawn from the research:1. The halophytes to be used in the vicinity of the water reservoir in orer to improve the ground for the introduction of new plants and prevent the water contaminatioin. 2. The halophytes to be used as a gound-cover which protects major structures nearby from being damaged by the salt content in the air. 3. Thd halophytes to be used as a unique and panoramic landscape element. They have various colors which change throughout a year. 4. Thd halophytes to be considered as a outdoor recreation resource. The halophytes planted area may be utilized as picnic area, outdoor event plaza, ball game ground, rough in the golf cource and etc. Additional usage of the land include the future rearch site for sea fronted ecological studies and pasture for cows and other livestock.

• Nuclear Engineering and Technology
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• v.51 no.5
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• pp.1279-1288
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• 2019

The vortex-type fluidic diode (FD) is a key safety component for inherent safety in various advanced reactors such as the sodium fast reactor (SFR) and the molten salt reactor (MSR). In this study, topology optimization is conducted to optimize the design of the vortex-type fluidic diode. The optimization domain is simplified to 2-dimensional geometry for a tangential port and chamber. As a result, a design with a circular chamber and a restrictor at the tangential port is obtained. To verify the new design, experimental study and computational fluid dynamics (CFD) analysis were conducted for inlet Reynolds numbers between 2000 and 6000. However, the results show that the performance of the new design is no better than the original reference design. To analyze the cause of this result, detailed analysis is performed on the velocity and pressure field using flow visualization experiments and 3-D CFD analysis. The results show that the discrepancy between the optimization results in 2-D and the experimental results in 3-D originated from exclusion of an important pressure loss contributor in the optimization process. This study also concludes that the junction design of the axial port and chamber offers potential for improvement of fluidic diode performance.