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Studies on the Development of Natural Preservatives from Natural Products (전통식품 및 천연물에서 천연보존료 개발에 관한 연구)

  • Kim, Hee-Yun;Lee, Young-Ja;Hong, Ki-Hyoung;Kwon, Yong-Kwan;Lee, Ju-Yeun;Kim, So-Hee;Ha, Sang-Chunl;Cho, Hong-Yon;Chang, Ih-Seop;Lee, Chul-Won;Kim, Kil-Saeng
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1667-1678
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    • 1999
  • Certain parts of 190 kinds of medicinal herbs and 171 kinds of original materials of food were extracted by methanol. The extracts were tested their microbial inhibition activities against several food spoilage microorganisms, Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli, Saccharomyces cerevisiae, Candida albicans, Penicillium citrinum, Aspergillus flavus and Aspergillus niger. The methanol extracts of Cornus officinalis, Evodia officinalis, Glycyrrhiza glabra, Salvia miltiorrhiza. Schizandrae fructus, Coptidis rhizoma, aroma hop and bitter hop were shown inhibitory effect on certain species of gram(+) bacteria. Aroma hop and bitter hop were shown inhibitory effect on certain species of gram(-) bacteria. The methanol extract of Salvia miltiorrhiza exhibited a strong antibacterial activities. It was purified by solvent fractionation, silicagel column chromatography, prep. TLC, prep. HPLC. The purified active substance was identified as cryptotanshinone by EIMS, $1^H-NMR,\;{13}^C-NMR$ and DEPT. Cryptotanshinone showed a strong antibacterial activity against gram positive bacteria $(MIC\;:\;3.91{\sim}62.50\;{\mu}g/mL)$. Especially, this compound was the most strong activity against Bacillus subtilis $(MIC\;:\;3.91\;{\mu}g/mL)$.

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Development of Analysis Method of Caffeine and Content Survey in Commercial Foods by HPLC (HPLC를 이용한 카페인의 분석법 개발 및 시판 식품중 함유량 조사)

  • Kim, Hee-Yun;Lee, Young-Ja;Hong, Ki-Hyoung;Lee, Chul-Won;Kim, Kil-Saeng;Ha, Sang-Chul
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1471-1476
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    • 1999
  • A simple and practical method for determination of caffeine in foods was developed. The analysis of caffeine was performed by reverse phase high performance liquid chromatography using a ${\mu}-Bondapak\;C_{18}$ column at isocratic condition with methanol-acetic acid-water(20 : 1 : 79) on UV detector at 280 nm. The clean-up and extraction of caffeine in samples were based on a simple pretreatment using a Sep-Pak $C_{18}$ cartridge. Recovery rates obtained with this method for cider, candy, cookie, milk, ice cream and persimmon leaf tea were 99.23%, 99.50%, 99.17%, 99.37%, 98.93% and 99.10% respectively. And the detection limit of caffeine was $0.1\;{\mu}g/mL$. With this method, the range of caffeine contents extracted from coffee, green tea, black tea, Oolong tea(tea bag), soft drinks, ice cream, milk and commercial confectionery were $3.38{\sim}37.50\;mg/g,\;16.30{\sim}26.10\;mg/g,\;10.80{\sim}16.65\;mg/g,\;11.25\;mg/g,\;0.06{\sim}0.11\;mg/g,\;0.04{\sim}0.44\;mg/g,\;0.04{\sim}0.39\;mg/g\;and\;0.10{\sim}1.80\;mg/g$, respectively. But caffeine was not detected in the other tea such as Acanthopanax sessiliflorum tea, Angelica gigas tea, Angelica tea, Arrow root tea, Duchu'ng tea, Dunggulle tea, Ganoerma lucidum tea, Ginger tea powder, Persimmon leaf tea, Ssanghwa tea and Cocoa mix powder.

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Environmental Condition for the Butt-Rot of Conifers by Cauliflower Mushroom (Sparassis crispa) and Wood Quality of Larix kaempferi Damaged by the Fungus (꽃송이버섯에 의한 침엽수 심재부후 발생환경 및 낙엽송 피해목의 재질 특성)

  • Park, Hyun;Oh, Deuk-Sil;Ka, Kang Hyeon;Ryu, Sung-Ryul;Park, Joo-Saeng;Hwang, Jaehong;Park, Jun-Mo
    • Journal of Korean Society of Forest Science
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    • v.98 no.1
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    • pp.16-25
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    • 2009
  • Cauliflower mushroom (Sparassis crispa) is recently recognized as a new edible and/or medicinal mushroom cultivated with conifers. By the way, the mushroom is notorious as a brown-rot fungus that causes a buttrot of larch. So, there should be a careful consideration to apply the mushroom cultivation in coniferous stand. This study was conducted to clarify the seriousness of heartwood decay on conifers such as larch by cauliflower mushroom with surveying the mushroom producing environment and to examine whether the cultivation of cauliflower mushroom produce any problem in conifer stands or not. The mushroom occurred in various coniferous stands such as Larix kaempferi, Pinus koraiensis, P. densiflora and Abies holophylla on fertile soils with adequate moisture. Soil texture of the mushroom producing site was comparatively fine compared to general forest soils; sandy loam, loam and silty loam. Soil pH ranged from 4.6 to 5.2, and organic matter contents were 4~11%, which showed relatively wide range. We could find S. crispa by a DNA technique from the wood that seemed to have no heartwood decay by naked eyes. The damaged wood showed 30% higher moisture contents than that of sound wood, while the compressive strength was 30% lowered down compared to that of sound wood. The fungus may invade conifers through the scars occurred on roots or stems, in this case spore dispersion of the mushroom takes a great role. Thus, we concluded that forest tending activities need to be applied with considering the invasion of S. crispa, and cultivation of cauliflower mushroom in forest should be attempted very carefully. By the way, we also infer that conifer stands can be nurtured without heartwood decay by S. crispa if the stand be managed in good aeration conditions by proper silvicultural practices such as sanitary thinning.

Antioxidant Components and Antioxidant Activities of Methanolic Extract from Adzuki Beans (Vigna angularis var. nipponensis) (품종별 팥 메탄올 추출물의 항산화성분 및 항산화활성)

  • Woo, Koan-Sik;Song, Seuk-Bo;Ko, Jee-Yeon;Seo, Myung-Chul;Lee, Jae-Saeng;Kang, Jong-Rae;Oh, Byeong-Geun;Nam, Min-Hee;Jeong, Heon-Sang;Lee, Jun-Soo
    • Korean Journal of Food Science and Technology
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    • v.42 no.6
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    • pp.693-698
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    • 2010
  • In this study, the antioxidant compounds and antioxidant activities of adzuki beans were measured to evaluate their functional properties and to compare them to Daepung and Taekwang. Proximate compositions and mineral contents of the various adzuki beans were not significantly different. A high level of dietary fiber (14.81%) was found in Chilbopat. The extraction yields of Daepung and Taekwang were 38.70 and 34.61%, respectively; however the yields of the various adzuki beans were lower at 13.78-20.76%. The total polyphenol and flavonoid content of the various adzuki beans were 19.00-34.75 and 12.97-28.95 mg/g, respectively (Daepung: 14.40 and 3.96 mg/g, Taekwang: 10.7 and 2.61 mg/g). High levels of polyphenols (34.75 mg/g) and flavonoids (28.95 mg/g) were found in Miryang 6. The total anthocyanin and proanthocyanidin contents of the various adzuki beans ranged from 5.89-23.77 and 1.83-3.29 mg/g, respectively. A high level of total tocopherol content (7.66 mg/100 g) was found in Yungum-pat. High levels of ABTS radical (2.44 mg TEAC/mg extract residue) and DPPH radical scavenging activities (4.46 mg TEAC/mg extract residue) were found in Miryang 6. A significant correlation was also noted between free radical scavenging activity and polyphenolic compound content. The results of this study suggest that notable antioxidant activities in various adzuki beans could have significant health benefits.

Physicochemical Characteristics of Korean Traditional Wine Made from Proso Millet (Panicum miliaceum L.) at Different Addition Rates with Two Kinds of Nuruk (누룩 및 기장 첨가량을 달리한 발효주의 이화학적 특성)

  • Woo, Koan-Sik;Song, Seuk-Bo;Lee, Jea-Saeng;Ko, Jee-Yeon;Kang, Jong-Rae;Oh, Byeong-Geun;Nam, Min-Hee;Ryu, In-Soo;Seo, Myung-Chul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.2
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    • pp.119-125
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    • 2010
  • This study was carried out to compare the physicochemical characteristics of Korean traditional wine fermented from proso millet and nuruk at different mixing rate. The alcohol contents of the fermented wine ranged from 12.36 to 13.21%. Two kinds of nuruk, SH and BS nuruks were used. The brix degrees of proso millet wine fermented by SH and BS nuruks were 21.6 and 22.4 $^{\circ}Bx$, respectively. The pH, total acidity, and turbidity the wines fermented by SH and BS nuruks were 3.74 and 3.40, 1.40 and 1.51%, and 0.441 and 0.149, respectively. With increasing the addition amount of proso millet, brix degree, pH, turbidity L-value decreased, and total acidity and avalue increased. Total color difference (${\Delta}Eab$) parameter of the wine fermented by SH nuruk were 4.33, 6.63 and 26.13, and by BS nuruk were 4.08, 5.29 and 10.59 in 30, 70 and 100% addition rates of proso millet. With increasing the addition amount of proso millet, glucose content decreased. The organic acids such as lactic acid and acetic acid were predominantly detected in the fermented wine. Finally, based on sensory evaluations, the wine fermented by SH nuruk showed the best overall quality at 30% addition rate of proso millet.

Changes of Soil-Emission Gases and Microbial Diversity by Different Fertilizers Supplemented after Application of Livestock-Manure Compost in Greenhouse Soil (시설재배지(施設栽培地)에서 축분퇴비(畜糞堆肥) 시용시(施用時) 보충비종(補充費種)에 따른 토양배출(土壤排出)가스 및 미생물다양성(微生物多樣性)의 변화(變化))

  • Kang, Hang-Won;Ko, Jee-Yeon;Park, Hyang-Mee;Lee, Jae-Saeng;Rang, Ui-Gum;Park, Kyeong-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.1
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    • pp.52-60
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    • 2000
  • This study was conducted to gain basic data for alleviation of gas emission and conservation of healthy soil environment by investigating an aspect of gas emission and microbial diversity due to the supplement of different fertilizers after application with a livestock manure compost in greenhouse soils. Green pepper was cultivated in clay loamy soil from April to August. Before planting, a livestock manure compost was applied with $741mg\;ha^{-1}$ on the basis of the phosphate content contained in compost. And then, deficient nitrogen for cropping was supplemented with either quick-acting fertilizer of urea or a controlled slow release fertilizer made from urea formaldehyde(U/F). $NH_3$ and R $NH_2$ gases emitted from soil showed a low concentration in the early stage but a maximum in 27 days after planting, then decreased rapidly and not detected after 33 days. Their average concentrations were 42% and 85% lower in the treatment of slow release fertilizer than that of urea fertilizer, respectively. $CO_2$ gas emitted under urea fertilization was ranged from 1,200 to $3,200mg{\ell}^{-1}$ and that in slow release fertilizer was $900{\sim}2,650mg\;{\ell}^{-1}$. The average concentration of urea treatment was $2,260mg{\ell}^{-1}$ and 30% higher than that of slow release fertilizer. The treatment of slow release fertilizer with the lapse of cropping time populated larger in numbers of bacteria, actinomycetes, nitrate bacteria and nitrate reduction bacteria, and ratios of bacteria and actinmycetes to fungi than that of urea fertilizer. But the number of fungi was higher in the treatment of urea fertilizer and denitrifying bacteria showed a similar trend in both treatments. The microbial diversity index, which calculated with numbers of 6 species of microorganisms, was decreased with increasing of growing stage in the range of 0.1 to 0.35 and that was higher in the tratment of slow release fertilizer than urea.

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Effects of Cultural Practices on Methane Emission in Tillage and No-tillage Practice from Rice Paddy Fields (논토양에서 경운 및 무경운재배시 재배방법별 메탄 배출 양상)

  • Ko, Jee-Yeon;Lee, Jae-Saeng;Kim, Min-Tae;Kang, Hang-Won;Kang, Ui-Gum;Lee, Dong-Chang;Shin, Yong-Gwang;Kim, Kun-Yeop;Lee, Kyeong-Bo
    • Korean Journal of Soil Science and Fertilizer
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    • v.35 no.4
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    • pp.216-222
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    • 2002
  • Field experiments were conducted to investigate the effect of various cultural practices on methane($CH_4$) emission in tillage and no-tillage practice in a clayey paddy soil from 1998 to 2000. The factors evaluated in tillage and no-tillage methods were types of nitrogen fertilizers, application method of chemical fertilizers, rice straw application and cultivation method. Of the nitrogen fertilizers, the amount of $CH_4$ emission in ammonium sulfate plot was the lowest, regardless of tillage and the application method. 26.4~41.1% of reduction by ammonium sulfate compared with urea. But in no-tillage which have problem of poor rice yield than tillage, coated urea was more effective nitrogen fertilizer because that showed similar $CH_4$ emission and highest rice yield at 80% of dosage of nitrogen. No-tillage plot emitted lower $CH_4$ than tillage plot where the fertilizers were incorporated. On the contrary, no-tillage plot showed a little higher $CH_4$ emission compared with tillage plot for the surface application. When rice straw was applied, no-tillage practice reduced methane emission by 26.6% compared with tillage practice, but showing a little difference of 10.7% in no application. With cultivation method, no-tillage practice reduced methane emission 26.6% compared with tillage for the 30-d-old seedling transplanting. But for the dry direct seeding practice, no-tillage was a less effective because considerable amounts of rice straw incorporated by tillage were more decomposed aerobically in the soil and emitted as $CO_2$ to the atmosphere with flooding in no-tillage soil.

Intake of Food Additives in Foods by Total Diet (식이를 통한 식품첨가물의 섭취량)

  • Kim, Hee-Yun;Lee, Young-Ja;Hong, Ki-Hyoung;Ha, Sang-Chul;Ahn, Myung-Su;Jo, Jae-Sun;Kim, Kil-Saeng
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.767-774
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    • 1998
  • This study has been carried out to measure the amount of the daily intake by Korean on preservatives (Dehydroacetic acid, Benzoic acid and ρ-Hydroxybenzoic acid asters) in foods. The amounts of preservatives were determined by HPLC in foods such as butter, cheese, margarine, aloe gel, carbonated beverages, mixed beverage, ginseng beverage, red ginseng drink, soy sauce, fruit and vegetable juices (except unheated fruit-vegetable juice), vineger, seasonings, fruit and vegetables only on the peel, fruit wine, rice wine and unrefined rice wine. The recovery ranges were found to be 99.2% for dehydroacetic Acid, 95.8% for benzoic acid and $94.2{\sim}97.2%$ for ${\rho}-hydroxybenzoic$ acid in foods, respectively. The data of average food intake for each food items per capita per day were obtained from the report of national nutrition survey carried by the Ministry of Health and Welfare in 1997. The detected number of samples and average intake range of preservatives have been figured cut to $ND{\sim}290.0{\;}ppm$ for dehydroacetic acid, $ND{\sim}400.0{\;}ppm$ for benzoic acid and $ND{\sim}93.9{\;}ppm$ for ${\rho}-hydroxybenzoic$ acid esters, respectively. Estimated daily intake (EDI) of each additives per capita per day were shown as follows; 1.56 mg for dehydroacetic acid, 2.25 mg for benzoic acid and 0.44 mg for ${\rho}-hydroxybenzoic$ acid esters in total respectively and these values were evaluated to be much lower than that of FAO/WHO's acceptable daily intake (ADI) $0{\sim}5{\;}mg/kg$ b.w./day for benzoic acid and $0{\sim}10{\;}mg/kg$ b.w./day for ${\rho}-hydroxybenzoic$ acid esters.

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The Modes of Place Rootedness on Geochang Mohyeonjeong and Supodae (거창 모현정과 수포대의 장소착근(場所着根) 방식)

  • Rho, Jae-Hyun;Kim, Hong-Gyun;Lee, Hyun-Woo
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.30 no.3
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    • pp.87-96
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    • 2012
  • This study aimed at empirically identifying how the cultural phenomena of localisation and attachment are implemented through Mohyeonjeong and Supodae at Gajo-myeon, Geochang-gun, Gyeongnam. 'Daehakdong', the name of the place where the Mohyeon-jeong and Supodae is located, has a meaning of the place where Geong-Pil Kim(金宏弼) the Hanhwondang(寒暄堂), one of the 5 eastern sages, and Yeo-Chang Jeong(鄭汝昌) the Ildu tought Neo-Confucianism. In addition, in case of Mt. Odo(1,134m) embracing the garden, the meaning of Odo is the five virtues in Confucianism, so we can see that Confucianism was strong in that area. The meaning of 'Mohyeon(慕賢)', "missing and thinking of sages", reflects the emotion of attachment to the place where people pay a tribute to the memory of Seon-Saeng Yang the Hwondu, one of the 5 eastern sages and the creator of Neo-Confucianism in Kyeongnam, and Suk-Ryang Choi(崔淑梁) the ancestors Pyeongchon. In addition, Odojae(吾道齋), Kijeok monument to pay a tribute to the memory of Pyeongchon, the persimmon tree symbolizing Hanhwondang, and Jidongam(志同巖) standing in front of Mohyeon pavillion represent the united wills of the above 3 people to show their Dohak(道學) spirit by practicing it, and also a reiterated expression of attachment to the place. 'Hwonduyangseonsaeng janggujiso' and 'Pyeongchonchoigong ganghakjiso(坪村崔公講學地所)' engraved on the rock of Myeongso Supodae where they gave a lecture of Neo-Confucianism to local Confucianists for many years and enjoyed nature make us to identify the intrinsic meaning of the location that was inherited in the memory of people. Along with this, most of the content of poetry, restoration records, and Sangryang articles are filled with the content reminding of the historical meaning and origin of Mohyeon-jeong and Supodae, so we can see from this that the place had the spatial meaning of Jangsujiso(藏修之所), 'the place of lecturing and communicating' and respecting ancient sages. This spatial tradition is the result of positive attachment to the place, and Mohyeon-jeong and Supodae is the place where the attachment to the place was made spontaneously througth the localisation. To sum it up, Mohyeon-jeong and Supodae was the place of attachment where one paid a tribute to the memory of ancient sages, and Mohyeonjeong and Supodae rocks were the representative examples of the localisation to show the meaning of the place by implication. Studying the process of attachment and localisation of the place does not only enable us to infer the genuine form of the traditional memorial space and park, but also to reproduce the place with the modern concept.

Peptide Properties of Rapid Salted and Fermented Anchovy Sauce Using Various Pretenses 1. Hydrolysis of Anchovy Sauce and Actomyosin by Various Pretenses (단백질 분해효소를 이용하여 제조한 속성 멸치 액젓의 펩티드 특성 1. 단백질 분해효소에 의한 멸치 액젓 및 Actomyosin의 가수분해)

  • KIM In-Soo;CHOI Young-Joon;HEU Min-Soo;CHO Young-Je;IM Yeong-Sun;GU Yeun-Suk;YEO Saeng-Gyu;PARK Jae-Woon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.4
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    • pp.481-487
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    • 1999
  • The optimal conditions of enzymatic hydrolysis for preparation of rapid salted and fermented anchovy sauce (SFAS) using various pretenses such as trypsin, chymotrypsin, crude enzyme from squid liver and viscera, Alcalase, Neutrase and Protamex were studied. SFAS prepared with squid viscera had higher level of VBN (173.6 mg/100 g) when stored for 70 days than other samples, and peroxide values were almost equal among all samples during fermentation period. Total amino acids and nonprotein nitrogenous compounds remarkably increased as SFAS treated with Alcalase or Protamex which exhibited higher the hydrolysis rate of $57\%$ at 60 day than others. The optimal pHs of trypsin, chymotryosin, Alcalase, Neutrase and Protamex on anchovy actomyosin were 7.5, 6.5, 6.5, 7.0 and 5.0, respectively. Optimal temperatures of trypsin, chymotryosin, Alcalase and Neutrase were 55, 45, 60 and $55^{\circ}C$, respectively. Otherwise, Protamex activity increased as temperature increased from 20 to $70^{\circ}C$. Protamex had higher $K_m$ (3.545) and $V_{max}$ value (2.688) than others. Protamex affected less by NaCl had $52.5\%$ activity at the fermentation condition of $20^{\circ}C$ and $25\%$ NaCl. Protamex appeared to be very effective for the hydrolysis of crude actomyosin from ancnovy.

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