Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant Components and Antioxidant Activities of Methanolic Extract from Adzuki Beans (Vigna angularis var. nipponensis)

품종별 팥 메탄올 추출물의 항산화성분 및 항산화활성

  • Woo, Koan-Sik (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Song, Seuk-Bo (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Ko, Jee-Yeon (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Seo, Myung-Chul (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jae-Saeng (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Kang, Jong-Rae (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Byeong-Geun (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Nam, Min-Hee (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Department of Food Science and Technology, Chungbuk National University)
  • 우관식 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 송석보 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 고지연 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 서명철 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이재생 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 강종래 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 오병근 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 남민희 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 이준수 (충북대학교 식품공학과)
  • Received : 2010.09.13
  • Accepted : 2010.10.07
  • Published : 2010.12.31
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Abstract

In this study, the antioxidant compounds and antioxidant activities of adzuki beans were measured to evaluate their functional properties and to compare them to Daepung and Taekwang. Proximate compositions and mineral contents of the various adzuki beans were not significantly different. A high level of dietary fiber (14.81%) was found in Chilbopat. The extraction yields of Daepung and Taekwang were 38.70 and 34.61%, respectively; however the yields of the various adzuki beans were lower at 13.78-20.76%. The total polyphenol and flavonoid content of the various adzuki beans were 19.00-34.75 and 12.97-28.95 mg/g, respectively (Daepung: 14.40 and 3.96 mg/g, Taekwang: 10.7 and 2.61 mg/g). High levels of polyphenols (34.75 mg/g) and flavonoids (28.95 mg/g) were found in Miryang 6. The total anthocyanin and proanthocyanidin contents of the various adzuki beans ranged from 5.89-23.77 and 1.83-3.29 mg/g, respectively. A high level of total tocopherol content (7.66 mg/100 g) was found in Yungum-pat. High levels of ABTS radical (2.44 mg TEAC/mg extract residue) and DPPH radical scavenging activities (4.46 mg TEAC/mg extract residue) were found in Miryang 6. A significant correlation was also noted between free radical scavenging activity and polyphenolic compound content. The results of this study suggest that notable antioxidant activities in various adzuki beans could have significant health benefits.

본 연구에서는 품종별 팥 메탄올 추출물의 항산화성분과 항산화활성을 비교 분석하고자 하였다. 항산화성분으로 총 polyphenol, flavonoid, anthocyanin 및 proanthocyanidin 함량을 측정하였으며, 항산화활성은 ABTS와 DPPH radical 소거활성을 측정하였다. 품종별 팥의 일반성분과 무기성분의 함량은 품종간에 큰차이를 보이지 않았으며, 식이섬유 함량은 품종간에 유의적인 차이를 보였고 특히 칠보팥(14.81%)의 식이섬유 함량이 가장 높게 나타났다. 추출수율은 태광콩과 대풍콩이 각각 38.70 및 34.61%로 높은 추출수율을 보였으며, 칠보팥, 금실팥 및 밀양8호가 각각 20.76, 20.33 및 20.16%로 비교적 높은 수율을 보였고 중부팥이 13.78%로 가장 낮은 수율을 나타내었다. 품종별 팥 추출물의 총 polyphenol 및 flavonoid 함량은 각각 19.00-34.75 및 12.97-28.95 mg/g으로 대조군보다 높은 함량을 보이는 것으로 나타났으며, 특히 밀양6호는 각각 34.75 및 28.95mg/g으로 가장 높은 함량을 보였다. 품종별 팥 추출물의 총 anthocyanin 및 proanthocyanidin 함량은 각각 5.89-23.77 및 1.83-3.29 mg/g으로 나타나 대조군보다 높은 함량을 보였다. 총 tocopherol 함량은 5.71-7.66 mg/100 g으로 나타났으며, 연금팥이 7.66 mg/100 g으로 가장 높은 함량을 보였다. ABTS와 DPPH radical 소거활성은 밀양6호 추출물이 각각 2.44 및 4.46 mg TEAC/mg extract residue로 가장 높은 활성을 보였다. 이상의 결과를 볼 때 국내에서 개발된 팥 품종은 높은 항산화성분과 우수한 항산화활성을 가지는 것으로 나타나 천연항산화제 개발에 이용될 수 있을 것으로 생각한다.

Keywords

References

  1. Rho CW, Son SY, Hong ST, Lee KH, Ryu IM. Agronomic characters of Korean adzuki beans (Vigna angularis (Willd.) Ohwi & Ohashi). Korean J. Plant Res. 16: 147-154 (2003)
  2. Chang KY Han KS, Park JC. Studies on the selection in adzuki bean breeding. III. Phenotypic and genotypic correlations among some characters in the population of adzuki bean varieties. Res. Bull. Chinju Agric. College, Korea 7: 39-44 (1968)
  3. Koh KJ, Shin DB, Lee YC. Phsicochemical properties of aqueous extracts in small red bean, mung bean and black soybean. Korean J. Food Sci. Technol. 29: 854-859 (1997)
  4. Hwang CS, Jeong DY, Kim YS, Na JM, Shin DH. Effects of enzyme treatment on physicochemical characteristics of small red bean percolate. Korean J. Food Sci. Technol. 37: 189-193 (2005)
  5. Kim CG, Oh BH, Na JM, Sin DH. Comparison of physicochemical properties of Korean and Chinese red bean starches. Korean J. Food Sci. Technol. 35: 551-555 (2003)
  6. Chang HG. Information on Food for the Health of Modern People. Shinkwang Publishing Co., Seoul, Korea. pp. 46-50 (1999)
  7. Choi SY, Jeong YJ, Lee SJ, Chi OH, Chegal SA. Food and Health for Modern People. Dongmyungsa, Seoul, Korea. pp. 244- 246 (2002)
  8. Yoshida K, Sato Y, Okuno R, Kameda K, Isobe M, Kondo T. Structural analysis and measurement of anthocyanin from colored seed coats of Vigna, Phaseolus, and Glycine Lugumes Biosci. Biotech. Bioch. 60: 589-593 (1996) https://doi.org/10.1271/bbb.60.589
  9. Ariga T, Koshiyama I, Fukushima D. Antioxidative properties of procyanidins B-1 and B-3 from azuki beans in aqueous systems. Agr. Biol. Chem. 52: 2717-2722 (1988) https://doi.org/10.1271/bbb1961.52.2717
  10. Koide T, Hashimoto Y, Kamei H, Kojima T, Hasegawa M, Terabe K. Antitumor effect of anthocyanin fractions extracted from red soybeans and red beans in vitro and in vivo. Cancer Biother. Radio. 12: 277-280 (1997)
  11. Kim HJ, Sohn KH, Park HK. Emulsion properties of small red bean protein isolates. Korean J. Food Cookery Sci. 6: 9-14 (1990)
  12. Meng GT, Ma CY. Flow property of globulin from red bean (Phaseolus angularis). Food Res. Int. 34: 401-407 (2001) https://doi.org/10.1016/S0963-9969(00)00184-8
  13. Meng GT, Ma CY. Thermal properties of Phaseolus angularis (red bean) globulin. Food Chem. 73: 453-460 (2001) https://doi.org/10.1016/S0308-8146(00)00329-0
  14. Abu-Ghannam N. Modelling textural changes during the hydration process of red beans. J. Food Eng. 38: 341-352 (1998) https://doi.org/10.1016/S0260-8774(98)00127-7
  15. Dewanto V, Xianzhong W, Liu RH. Processed sweet corn has higher antioxidant activity. J. Agr. Food Chem. 50: 4959-4964 (2002) https://doi.org/10.1021/jf0255937
  16. Turker N, Erdogdu F. Effects of pH and temperature of extraction medium on effective diffusion coefficient of anthocyanin pigments of black carrot (Daucus carota var. L.). J. Food Eng. 76: 579-583 (2006) https://doi.org/10.1016/j.jfoodeng.2005.06.005
  17. Baoshan S, Jorge M, Ricardo DS, Isabel S. Critical factors of vanillin assay for catechins and proanthocyanidins. J. Agr. Food Chem. 46: 4267-4274 (1998) https://doi.org/10.1021/jf980366j
  18. Lee SM, Lee J. Tocopherol and tocotrienol contents of vegetable oils, margarines, butters, and peanut butters consumed in Korean diet. Food Sci. Biotechnol. 15: 183-188 (2006)
  19. Choi Y, Lee SM, Chun J, Lee HB, Lee J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake (Lentinus edodes) mushroom. Food Chem. 99: 381-387 (2006) https://doi.org/10.1016/j.foodchem.2005.08.004
  20. Kong S, Choi Y, Kim Y, Kim DJ, Lee J. Antioxidant activity and antioxidant components in methanolic extract from 'Geumjong' rice. J. Korean Soc. Food Sci. Nutr. 38: 807-811 (2009) https://doi.org/10.3746/jkfn.2009.38.6.807
  21. Zielinski H, Kozlowska H. Antioxidant activity and total phenolics in selected grains and their different morphological fractions. J. Agr. Food Chem. 48: 2008-2016 (2000) https://doi.org/10.1021/jf990619o
  22. Middleton E, Kandaswami C. Potential health-promoting properties of citrus flavonoids. Food Technol. -Chicago 48: 115-119 (1994)
  23. Elisia I, Hu C, Popovich DG, Kitts DD. Antioxidant assessment of an anthocyanin-enriched blackberry extract. Food Chem. 101: 1052-1058 (2007) https://doi.org/10.1016/j.foodchem.2006.02.060
  24. Hu C, Zawistowski J, Ling W, Kitts DD. Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J. Agr. Food Chem. 51: 5271-5277 (2003) https://doi.org/10.1021/jf034466n
  25. John S, Jianmel Y, Joseph E, Yukio K. Polyphenolics in grape seeds-biochemistry and functionality. J. Med. Food 6: 291-299 (2003) https://doi.org/10.1089/109662003772519831
  26. Hwang IW, Lee HR, Kim SK, Zheng H Z, Choi JU, Lee SH, Lee SH, Chung SK. Proanthocyanidin content and antioxidant characteristics of grape seeds. Korean J. Food Preserv. 15: 859-863 (2008)
  27. Qureshi AA, Mo H, Packer L, Peterson DM. Isolation and identification of novel tocotrienols from rice bran with hypocholesterolemic, antioxidant, and antitumor properties. J. Agr. Food Chem. 48: 3130-3140 (2000) https://doi.org/10.1021/jf000099t
  28. Kim JE, Joo SI, Seo JH, Lee SP. Antioxidant and a-glucosidase inhibitory effect of tartary buckwheat extract obtained by the treatment of different solvents and enzymes. J. Korean Soc. Food Sci. Nutr. 38: 989-995 (2009) https://doi.org/10.3746/jkfn.2009.38.8.989
  29. Nieva MM, Sampietro AR, Vattuone MA. Comparison of the free radical-savenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71: 109-114 (2000) https://doi.org/10.1016/S0378-8741(99)00189-0
  30. Kim SM, Cho YS, Sung SK. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J. Food Sci. Technol. 33: 626-632 (2001)
  31. Kang YH, Park YK, Lee GD. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J. Food Sci. Technol. 28: 624-630 (1996)