• 제목/요약/키워드: s disease (AD)

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금은화, 연교 및 황련해독탕 약침이 아토피 피부염 유발 NC/Nga 생쥐에 미치는 영향 (The Effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacopuncture on Atopic Dermatitis in NC/Nga Mice)

  • 유정석;김종욱;이창현;이상룡;육태한
    • Journal of Acupuncture Research
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    • 제32권4호
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    • pp.119-131
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    • 2015
  • Objectives : Atopic dermatitis is a chronic inflammatory skin disease characterized by pruritic and erythematous skin lesions. The purpose of this study was to investigate the suppressive effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacopuncture on the development of atopic dermatitis-like skin lesions in NC/Nga Mice. Methods : The Atopic Dermatitis was induced by biostir AD on the mice's back skin. Experimental groups were divided into three including LFP(Lonicerae Flos Pharmacopuncture, EtOH extract), FFP(Forsythiae Fluctus Pharmacopuncture, EtOH extract) and HHP(Hwangryunhaedok Decoction Pharmacopuncture, Hydrodistillation extract). Every second day, the mice of three groups were treated with $0.1m{\ell}$ of pharmacopuncture using a syringe at right and left acupoints ($BL_{13}$), alternatively. On the control group, normal saline was used instead of pharmacopuncture. Subsequently optical observation with a handscope, a clinical skin score, Tissue(general/immune) mast cell, Serum IgE level, Serum histamine level, and Serum lymphokine(IL-2, IL-4, $IFN-{\gamma}{\gamma}$) were measured. Results : FFP and HHP decreased the clinical skin score, the total cell number of mast cells, and the Serum total IgE level and Serum histamine level. In Serum lymphokine levels, all groups were decreased to the IL-4 level, LFP and FFP were increased to the IL-2 level, and LFP was increased to the $IFN-{\gamma}$ level. Conclusions : From the above results, Forsythiae Fluctus Pharmacopuncture (EtOH extract) and Hwangryunhaedok Decoction Pharmacopuncture (Hydrodistillation extract) exerted anti-allergic and anti-inflammatory effects, suggesting a promising agent for improving atopic dermatitis related symptoms.

Intra-Rater and Inter-Rater Reliability of Brain Surface Intensity Model (BSIM)-Based Cortical Thickness Analysis Using 3T MRI

  • Jeon, Ji Young;Moon, Won-Jin;Moon, Yeon-Sil;Han, Seol-Heui
    • Investigative Magnetic Resonance Imaging
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    • 제19권3호
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    • pp.168-177
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    • 2015
  • Purpose: Brain surface intensity model (BSIM)-based cortical thickness analysis does not require complicated 3D segmentation of brain gray/white matters. Instead, this technique uses the local intensity profile to compute cortical thickness. The aim of the present study was to evaluate intra-rater and inter-rater reliability of BSIM-based cortical thickness analysis using images from elderly participants. Materials and Methods: Fifteen healthy elderly participants (ages, 55-84 years) were included in this study. High-resolution 3D T1-spoiled gradient recalled-echo (SPGR) images were obtained using 3T MRI. BSIM-based processing steps included an inhomogeneity correction, intensity normalization, skull stripping, atlas registration, extraction of intensity profiles, and calculation of cortical thickness. Processing steps were automatic, with the exception of semiautomatic skull stripping. Individual cortical thicknesses were compared to a database indicating mean cortical thickness of healthy adults, in order to produce Z-score thinning maps. Intra-class correlation coefficients (ICCs) were calculated in order to evaluate inter-rater and intra-rater reliabilities. Results: ICCs for intra-rater reliability were excellent, ranging from 0.751-0.940 in brain regions except the right occipital, left anterior cingulate, and left and right cerebellum (ICCs = 0.65-0.741). Although ICCs for inter-rater reliability were fair to excellent in most regions, poor inter-rater correlations were observed for the cingulate and occipital regions. Processing time, including manual skull stripping, was $17.07{\pm}3.43min$. Z-score maps for all participants indicated that cortical thicknesses were not significantly different from those in the comparison databases of healthy adults. Conclusion: BSIM-based cortical thickness measurements provide acceptable intra-rater and inter-rater reliability. We therefore suggest BSIM-based cortical thickness analysis as an adjunct clinical tool to detect cortical atrophy.

초고압 발효 더덕 추출물의 인지능력 개선 효과 (Memory Enhancing Effect of Codonopsis lanceolata by High Hydrostatic Pressure Process and Fermentation)

  • 원진배;이보형;윤보라;이지우;이현용;박동식;정희철;정재윤;마충제
    • 생약학회지
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    • 제44권1호
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    • pp.41-46
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    • 2013
  • Alzheimer's disease (AD), most common form of dementia is characterized that memory deficit and loss of cognitive function. The Codonopsis lanceolata (C.lanceolata) was treated by high hydrostatic pressure process and fermentation. This study was evaluated cognitive enhancing effect C.lanceolata extract by high hydrostatic pressure process and fermentation and compared with common C.lanceolata extract using Morris water maze and passive avoidance test. And their neuroprotective effect on glutamate induced oxidative stress in HT22 cell was investigated by MTT assay. High hydrostatic pressure process and fermented C.lanceolata extract (HFCE) and common C.lanceolata extract (CCE) (100 and 300 mg/kg) were administered to mice. Results showed HFCE enhanced cognitive function than CCE as shown by decrease in escape latency time. HFCE increased the latency time of the passive avoidance test compared to CCE. Furthermore, HFCE showed significant neuroprotective effect against glutamate cytotoxicity in HT22 cells. These results indicate that high hydrostatic pressure process and fermented more improve spatial cognitive ability of C. laanceolata.

The Chloroform Fraction of Carpinus tschonoskii Leaves Inhibits the Production of Inflammatory Mediators in HaCaT Keratinocytes and RAW264.7 Macrophages

  • Kang, Gyeoung-Jin;Kang, Na-Jin;Han, Sang-Chul;Koo, Dong-Hwan;Kang, Hee-Kyoung;Yoo, Byoung-Sam;Yoo, Eun-Sook
    • Toxicological Research
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    • 제28권4호
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    • pp.255-262
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    • 2012
  • Inflammation is the immune system's response to infection and injury-related disorders, and is related to pro-inflammatory factors (NO, $PGE_2$, cytokines, etc.) produced by inflammatory cells. Atopic dermatitis (AD) is a representative inflammatory skin disease that is characterized by increasing serum levels of inflammatory chemokines, including macrophage-derived chemokine (MDC). Carpinus tschonoskii is a member of the genus Carpinus. We investigated the anti-inflammatory activity of C. tschonoskii by studying the effects of various solvent fractions prepared from its leaves on inflammatory mediators in HaCaT and RAW264.7 cells. We found that the chloroform fraction of C. tschonoskii inhibited MDC at both the protein and mRNA levels in HaCaT cells, acting via the inhibition of STAT1 in the IFN-${\gamma}$ signaling pathway. In addition, the chloroform fraction significantly suppressed the expression of inflammatory factors induced by lipopolysaccharide stimulation, except COX-2 and TNF-${\alpha}$. These results suggest that the chloroform fraction of C. tschonoskii leaves may include a component with potential anti-inflammatory activity.

더덕 물 추출물과 에탄올 추출물의 인지능 개선 활성 비교 (Effect of Water and Ethanol Extracts Codonopsis lanceolata on Spatial Learning and Memory in Mice)

  • 원진배;이지우;엄민례;정윤식;고현정;이현용;박동식;정희철;정재윤;마충제
    • 약학회지
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    • 제58권5호
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    • pp.287-293
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    • 2014
  • Alzheimer's disease (AD), most common form of dementia is characterized that memory deficit and loss of cognitive function. This study was evaluated cognitive enhancing effect of water and ethanol extracts of Codonopsis lanceolata and compared using Morris water maze and passive avoidance test. The water and 70% ethanol extracts (100, 300 and 500 mg/kg) were administered to mice. The neuroprotective effect on glutamate-induced cell death in HT22 cells was additionally investigated using MTT assay. Results showed 70% ethanol extract of Codonopsis lanceolata enhanced cognitive function than water extract, as shown by decrease in escape latency time in Morris water maze test. In passive avoidance test, 70% ethanol extract also increased the latency time compared to the water extract. Furthermore, 70% ethanol extract significantly protected neuronal cell against glutamate cytotoxicity and showed higher than neuroprotective effect of water extract. These results indicate that 70% ethanol extract more improve spatial cognitive ability and protected neuronal cells than water extract.

머신러닝을 이용한 의료 및 광고 블로그 분류 (A Classification of Medical and Advertising Blogs Using Machine Learning)

  • 이기성;이종찬
    • 한국산학기술학회논문지
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    • 제19권11호
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    • pp.730-737
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    • 2018
  • 행복한 삶의 질을 목적으로 하는 의료소비자가 증가하면서 웹에 분산되어 있는 블로그의 의료 정보를 바탕으로 신뢰성 있는 의료 시설을 선택하고 고품질의 의료 서비스를 받음으로서, 시간과 비용을 절약할 수 있는 O2O 의료 마케팅 시장이 활성화 되고 있다. 인터넷, 모바일, SNS 등에서 증가하는 비정형 텍스트 데이터는 전문 의료 지식 이외에 작성자의 관심, 선호, 예상 등을 직간접적으로 반영하고 있기 때문에 의료정보의 신뢰성을 담보하기 어렵다. 본 연구에서는 빅데이터 및 MLP를 사용하여 의료정보 블로그를 분류 (의료블로그, 광고블로그)함으로서 사용자에게 보다 고품질의 의료정보 서비스를 제공하는 블로그 판단 시스템을 제안한다. 제안된 빅데이터 및 머신러닝 기술을 통해 인터넷상에 존재하는 국내의 다수 의료정보 블로그를 종합, 분석한 후 질환별 개인 맞춤형 건강정보 추천 시스템을 개발한다. 이를 통하여 사용자는 자신의 건강문제를 지속적으로 점검하고 가장 적절한 조치를 취함으로서 자신의 건강 상태를 유지하는 것이 가능할 것으로 기대된다.

18F-THK5351 PET Imaging in the Behavioral Variant of Frontotemporal Dementia

  • Nam, Gijin;Jeong, Hye Jin;Kang, Jae Myeong;Lee, Sang-Yoon;Seo, Seongho;Seo, Ha-Eun;Park, Kee Hyung;Yeon, Byeong Kil;Ido, Tatsuo;Shin, Dong Jin;Noh, Young
    • 대한치매학회지
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    • 제17권4호
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    • pp.163-173
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    • 2018
  • Background and Purpose: Behavioral variant frontotemporal dementia (bvFTD) is a subtype of frontotemporal dementia, which has clinical symptoms of progressive personality and behavioral changes with deterioration of social cognition and executive functions. The pathology of bvFTD is known to be tauopathy or TDP-43 equally. We analyzed the $^{18}F-THK5351$ positron emission tomography (PET) scans, which were recently developed tau PET, in patients with clinically-diagnosed bvFTD. Methods: Forty-eight participants, including participants with behavioral variant frontotemporal dementia (bvFTD, n=3), Alzheimer's disease (AD, n=21) and normal cognition (NC, n=24) who completed 3T magnetic resonance images, $^{18}F-THK5351$ PET scans, and detailed neuropsychological tests were included in the study. Voxel-wise statistical analysis and region of interest (ROI)-based analyses were performed to evaluate the retention of THK in bvFTD patients. Results: In the voxel-based and ROI-based analyses, patients with bvFTD showed greater THK retention in the prefrontal, medial frontal, orbitofrontal, anterior cingulate, insula, anterior inferior temporal and striatum regions compared to NC participants. Left-right asymmetry was noted in the bvFTD patients. A patient with extrapyramidal symptoms showed much greater THK retention in the brainstem. Conclusions: The distribution of THK retention in the bvFTD patients was mainly in the frontal, insula, anterior temporal, and striatum regions which are known to be the brain regions corresponding to the clinical symptoms of bvFTD. Our study suggests that $^{18}F-THK5351$ PET imaging could be a supportive tool for diagnosis of bvFTD.

Scopolamine으로 유도된 인지장애 마우스의 기억력 개선 효과에 대한 인삼, 생지황, 발효홍삼 추출물의 비교 연구 (Comparative Study of Ginseng Radix, Rehmanniae Radix and Fermented Red-ginseng Extracts Effects on Memory Improvement in Scopolamine-induced Memory Impaired Mice)

  • 김지현;김종욱;이창현
    • 동의생리병리학회지
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    • 제36권1호
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    • pp.11-17
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    • 2022
  • In this study, we investigated the effects of Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng extracts (FRG) on cognitive function in scopolamine-induced memory-impaired mice. We measured the effects of G, R, and FRG on the improvement of memory and cognition via behavior analysis. In addition, we measured the acetylcholinesterase (AChE) activity in the hippocampus of each group of mice. The expression of β-amyloid, Tau, and BDNF in the brain tissues were observed through immunohistochemical staining. Ginseng Radix (G) and Fermented red-ginseng (FRG) have effectively improved cognitive function in the water maze test. Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) have improved the willingness of mice to explore the new environment, as confirmed by Y maze test. In addition, immunohistochemical staining revealed that Ginseng Radix (G) decreased the expression of β-amyloid and Tau in the hippocampus. In addition, fermented red-ginseng (FRG) increased the expression of BDNF. Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) have decreased the concentration of acetylcholinesterase in the hippocampus as compared with the control group of mice. In conclusion, Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) are considered to have the potential for development as candidate drugs to control the progression of Alzheimer's disease (AD).

알츠하이머 치매 및 경도인지기능장애 환자에서 나이, 성별, 유전자형을 고려한 뇌 회백질 부피와 표준신경심리검사와의 상관관계 연구 (Investigation of the Correlation between Seoul Neuropsychological Screening Battery Scores and the Gray Matter Volume after Correction of Covariates of the Age, Gender, and Genotypes in Patients with AD and MCI)

  • 이승연;윤수영;김민지;이학영;류창우;장건호
    • Investigative Magnetic Resonance Imaging
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    • 제17권4호
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    • pp.294-307
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    • 2013
  • 목적 : 본 연구의 목적은 다양한 신경심리검사를 통해 알츠하이머 치매 및 경도인지장애 환자군과 정상 노인 대조군에서 뇌회색질 부피와 신경심리검사 (SNSB) 결과의 복셀 기반분석을 이용한 상관관계를 알아내는데 있다. 대상 및 방법 : 총 피험자는 75명으로, 정상노인 25명, 경도인지장애 환자 25명, 그리고 알츠하이머 치매 환자 25명이었다. 모든 피험자로부터 유전자검사, 표준신경심리검사 (SNSB), 해부학적인 삼차원 T1 강조영상을 자화준비 고속경사에코 시퀀스를 이용하여 얻었다. 각 피험자군에서 뇌 회색질의 용적변화와 신경심리검사 점수와의 상관관계를 관찰하기 위하여, 복셀기반과 관심영역 기반 방법을 이용하여 분할한 회색질 영상을 다중회기방식 (multiple regression)으로 통계처리 하였다. 이때 피험자 각각의 성별과 나이 및 유전자 보유형태를 공변량 (covariate) 값으로 넣어 그 차이를 고려하였다. 결과 : 알츠하이머 환자군에서는 레이 복합도형 그리기 지연회상 검사 (RCFT delayed recall) 점수가 낮을수록 뇌 회색질 용적이 감소했다. 경도인지 장애군에서는 서울 언어학습 검사 (SVLT) 점수가 낮을수록 뇌회색질 용적이 감소했다. 정상 피험자 군에서 한국형 보스턴 이름대기 검사 (K-BNT) 점수 및 한국형 간이정신상태 검사 (K-MMSE) 점수와 뇌 회색질 부피가 음의 상관관계가 있음을 보였고, 레이 복합도형 그리기 검사 (RCFT) 점수와는 양의 상관관계를 보여주고 있다. 결론 : 나이, 성별, 유전자형태를 공변량으로 사용하였을 때 뇌에서 신경심리검사 결과와 3D T1 강조영상에서 얻은 뇌 회색질 부피 사이에 통계적으로 유의한 상관관계가 있음을 밝혔다. 이들 피험자를 대상으로 하는 종적 연구가 이루어 져야 한다고 생각이 든다.

간호사 국가고시문제의 내용분석 (A Content Analysis of the test of the National Examination for Registration Nurses in Korea over 3 years)

  • 서문자;윤순녕;유지수;송지호;최경숙
    • 대한간호학회지
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    • 제26권1호
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    • pp.73-93
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    • 1996
  • This study aimed to analyse the test contents of the national examination for the registered nurses (NERN) over 3 years from 1991 to 1993 in Korea. In recent years in Korea, the MCQ(multiple choice question) has been showing to be a highly recognized method for assessing the qualification of registered nurses. Unfortunately, nursing faculties have found NERN had some bad MCQs through having evaluation workshop for Some MCQs often provide so many unwriting clues which become a bias of the results, and some items fell into the category of the lower level of educational taxonomy such as isolated recall a fact or data. Frequently the stems of the questions are ambigous, unclear, disputable, esoterical or trivial. Considering those fallacies of the national examination, it is very critical to review the test items to see whether it is of high quality, is more fair, reliable and objective in depth. Therefore, this study was done to provide data for the improvement of the test contents as well as the teachers's assessment skill. For this study, the ad hoc committee was composed of 16 members, including 5 education board members of Korean Academic Nurses Association and 11 nursing faculty members. This committee had one day panel discussion and filled the checklist for this study. The process of analysing data was held over 10 times during 1992-1994. The analysis focussed on educational taxonomy such as cognitive domain(knowledge), psychmotor domain (skill), affective domain(attitude) and the level of learning such as recall, understanding, problems solving, and learning area of theory and practice, and the learning content categorised by nursing process and disease process. The test analysed using difficulty index and the structure of the test items was analysed. The conclusions and suggestion as follows : 1. In learning area, the average ratio of the theory and practice was 1 : 1.1 which was less than 1 : 2 suggested by Korean National Health Institute, and the ratio was different by the 8 leaning subjects of nursing. 2. In category of the educational taxonomy, the knowledge domain was emphasized mostly(79. 7%), the skill domain was 14.9%, and the attitude domain was 5.4% only. 3. In the level of learning, generally, the test items of the level of recall(45.5%) and the understanding(46.3%) were covered almost and the problem solving was 8.1%. 4. In the learning contents, generally, the test items related to nursing process was 67.2% and that of disease process was 32.8%. However, this proportion was different by the 8 leaning subjects. Even though the nursing diagnosis has been emphasized in nursing curricula recently, the test items of this was identified very few. 5. In the structure of the test item, some were not clear, incorrect grammar, unclear description and some have clues to answer. 6. In the item analysis, the non-acceptable level of the difficulty index (means too easy) was 65.7%, and the acceptable level was 33.9%. Considering the results we would like to suggest the followings, 1. Since the test items of knowledge domain was dominant, the test items of the practice domain and attitude domain should be emphasized more. 2. The regular review and analysis of NERN should be arranged in order to improve the quality of the test items which will give influence to the nursing education positively.

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