Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacopuncture on Atopic Dermatitis in NC/Nga Mice

금은화, 연교 및 황련해독탕 약침이 아토피 피부염 유발 NC/Nga 생쥐에 미치는 영향

  • Yu, Jung Suk (Department of Acupuncture & Moxibustion Medicine, Korean Medicine Hospital of Woosuk University) ;
  • Kim, Jong Uk (Department of Acupuncture & Moxibustion Medicine, Korean Medicine Hospital of Woosuk University) ;
  • Lee, Chang Hyun (Department of Anatomy, College of Korean Medicine, Woosuk University) ;
  • Lee, Sang Ryong (Department of Meridian & Acupoint, College of Korean Medicine, Woosuk University) ;
  • Yook, Tae Han (Department of Acupuncture & Moxibustion Medicine, Korean Medicine Hospital of Woosuk University)
  • 유정석 (우석대학교 부속한방병원 침구의학과) ;
  • 김종욱 (우석대학교 부속한방병원 침구의학과) ;
  • 이창현 (우석대학교 해부학교실) ;
  • 이상룡 (우석대학교 경혈학교실) ;
  • 육태한 (우석대학교 부속한방병원 침구의학과)
  • Received : 2015.12.12
  • Accepted : 2015.12.15
  • Published : 2015.12.18
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Abstract

Objectives : Atopic dermatitis is a chronic inflammatory skin disease characterized by pruritic and erythematous skin lesions. The purpose of this study was to investigate the suppressive effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacopuncture on the development of atopic dermatitis-like skin lesions in NC/Nga Mice. Methods : The Atopic Dermatitis was induced by biostir AD on the mice's back skin. Experimental groups were divided into three including LFP(Lonicerae Flos Pharmacopuncture, EtOH extract), FFP(Forsythiae Fluctus Pharmacopuncture, EtOH extract) and HHP(Hwangryunhaedok Decoction Pharmacopuncture, Hydrodistillation extract). Every second day, the mice of three groups were treated with $0.1m{\ell}$ of pharmacopuncture using a syringe at right and left acupoints ($BL_{13}$), alternatively. On the control group, normal saline was used instead of pharmacopuncture. Subsequently optical observation with a handscope, a clinical skin score, Tissue(general/immune) mast cell, Serum IgE level, Serum histamine level, and Serum lymphokine(IL-2, IL-4, $IFN-{\gamma}{\gamma}$) were measured. Results : FFP and HHP decreased the clinical skin score, the total cell number of mast cells, and the Serum total IgE level and Serum histamine level. In Serum lymphokine levels, all groups were decreased to the IL-4 level, LFP and FFP were increased to the IL-2 level, and LFP was increased to the $IFN-{\gamma}$ level. Conclusions : From the above results, Forsythiae Fluctus Pharmacopuncture (EtOH extract) and Hwangryunhaedok Decoction Pharmacopuncture (Hydrodistillation extract) exerted anti-allergic and anti-inflammatory effects, suggesting a promising agent for improving atopic dermatitis related symptoms.

Keywords

References

  1. Lee JH, Kim KH, Kim MN et al. Report from ADRG : A Study on the Diagnostic Criteria of Korean Atopic Dermatitis. Korean J Dermatology. 2006 ; 44(8) : 907-13.
  2. Kim KH, Park CW, Eun HC, Cho SH. Atopic dermatitis. Dermatology. 5th ed. Seoul : Ryo Mon Gak . 2008 : 170-8.
  3. Park JH, Choi IH. The Effect of Herbal-Acupuncture Using Ursi Fel into Zusanli(ST36) to Recover Function of Stratum Corneum on Mice Model after Atopic Dermatitis Elicitation. J Korean Oriental Med. 2005 ; 26(3) : 13-26.
  4. Seo BJ. Effect of Bee Venom Acupuncture on Trimellitic Anhydride- induced Atopic Dermatitis in Mice [dissertation].Seoul : Kyunghee Univ. 2010. Korean.
  5. Kim MC. The Effects of Soyeom and Rehmanniae radix Pharmacopuncture on Atopic Dermatitis in NC/Nga Mice[dissertation]. Jeounju: Woosuk Univ. 2011. Korean.
  6. The Acupuncture and Moxibustion Medicine textbook compilation committee. The Acupuncture and Moxibustion Medicine(上). Paju : Jipmoondang. 2008 : 130-1.
  7. Vestergaard C, Yoneyama H, Matsushima K. The NC/Nga mouse : a model for atopic dermatitis. Molecular Medecine Today. 2000 ; 6(5) : 209-10. https://doi.org/10.1016/S1357-4310(00)01683-X
  8. Youn DH, Choi GJ, Chae WS, Na CS. Effects of Cordyceps militaris Mycelia(CMM) herbal acupuncture at BL13, LU4 on airway smooth muscle, airway inflammation, IgE and Interleukin-4 in mouse model of allergic bronchial asthma. Korean J Acupunct. 2004 ; 21(2) : 111-23.
  9. Jun HK, Chung JS, Seo YM, Park SK, Lee YW, Kim DH. Treatment of Canine Tracheal Collapse by Injection-Acupuncture and Herbal Medicine. J Vet Clin. 2007 ; 24(3) : 419-21.
  10. Yamamoto,M., Haruna, T., Ueda, C. et al.Contribution of itch-associated scratch behavior to the development of skin lesions in Dermatophagoides farinae-induced dermatitis model in NC/Nga mice. Arch Dermatol Res. 2009 ; 301(10) : 739-46. https://doi.org/10.1007/s00403-008-0912-8
  11. Enavall E, Perlmann P. Enzyme-linked immuno-sorbent assay III, Quantitation of specific antibodies of enzyme labelled anti- Immunoglobulin in antigen-coatedtubes. J Immunol. 1972 ; 109 : 129-35.
  12. Kim JJ, Yang SW, Son NW, Ann KS. Anti-inflammatory action by Gami-angryosamul- tang and the effect of Ziyang-Go on atopic dermatitis-like lesionand pruritus in NC/Nga mice. KJOMPP. 2003 ; 17(2) : 428-35.
  13. Hanifin JM, Rajka G. Diagnostic features of atopic dermatitis. Acta Dermato-Venereologica. Supplementum. 1980 ; 92 : 44-7.
  14. Leung DY, Boguniewicz M, Howell MD, Nomura I, Hamid QA. New insights into atopic dermatitis. J Clin Invest. 2004 ; 113(7) : 651-7. https://doi.org/10.1172/JCI21060
  15. The Korean Academy of Pediatric Allergy and Respiratory Disease. Pediatric Allergy and Respiratory disease. Seoul : Koonja publishing company. 2005 : 145-73.
  16. Kim DH, Kang KH, Kim KW, Yoo IY. Management of Children with Atopic Dermatitis. AARD. 2008 ; 18(2) : 148-57.
  17. Kim SC, An KJ, Han SG et al. Clinico-Epidemiologic study on the Abuse, Misuse, and Adverse Effects of Topical Dermatologic Drugs. Korean J Dermatol. 2003 ; 41(9) : 1129-35.
  18. Lee SJ, Lee MJ, Jung JE et al.Antimicrobial Effects of Fermented Coptidis rhizoma and Lonicerae Flos against pathogen. J Korean Med Obes Res. 2011 ; 11(1) : 35-46.
  19. Chung KJ, Jung HJ, Jung SK, Rhee HK. Lonicerae Flos contributes to the chemotaxis of eosinophils and secretion of cytokines in A549 human epithelial cells. Korean J. Orient. Int. Med. 2005 ; 26(1) : 129-42.
  20. Lee DE, Lee JR, Kim YW. Inhibition of Lipopolysaccharide-Inducible Nitric Oxide Synthase, TNF-${\alpha}$, IL-1${\beta}$and COX-2 Expression by Flower and Whole Plant of Lonicera japonica. KJOMPP. 2005 ; 19(2) : 481-9.
  21. Park HS. The Effects of Anti-cancer Response of Lonicerae Flos Herbal-acupuncture. The Acupuncture. 2005 ; 22(5) : 91-7.
  22. Lee JM, Choi SW, Cho SH, Rhee SJ. Effect of Forsythia Viridissima Extracts on Antioxidative System and Lipid Peroxidation of Liver in Rats Fed High-Cholesterol Diet. J Nutr Health. 2003 ; 36(10) : 990-6.
  23. Schinella GR, Tournier HA, Prieto JM, Mordujovich D, Rios JL. Antioxidant activity of anti-inflammatory plant extracts. Life Sciences. 2002 ; 70(9) : 1023-33. https://doi.org/10.1016/S0024-3205(01)01482-5
  24. Seo HS. The Experimental Study on Anti-bacterial Potency of Hwangryunhaedok-tang -acupuncture & extract on Staphylococcus aureus & Staphylococcus epidermidis. Journal of Pharmacopuncture. 2006 ; 9(2) : 99-103. https://doi.org/10.3831/KPI.2006.9.2.099
  25. Kim SM, Choi HS, Seo BI. The Effect of Hwangryunhaedoktang on the Toxicity of Dried Mylabris phalerata Extract. Kor. J. Herbology. 2010 ; 25(2) : 41-54.
  26. Yang HJ, Joo HA, Baek SC, Park JS, Hong SH. Anti-inflammatory effects of Hwangnyeonhaedok-tang and Fermented Hwangnyeonhaedok-tang. J Korean Med Ophthalmol Otolaryngol Dermatol. 2011 ; 24(2) : 1-15.
  27. Choi JM, Kim HT. Effects of Hwangryunhaedok-tang on TNF-$\alpha$ and IL-4 Stimulated TARC, eotaxin, RANTES in the Human Bronchial Epithelial A549 Cells. KJOMPP. 2006 ; 20(6) : 1649-53.
  28. Seol H, Yook TH. Effects of Hwangryunhaedok-tang -acupuncture at G21(Kyonjong) on the Heart Rate Variability. The Acupuncture. 2004 ; 21(6) : 37-50.
  29. Weber A, KnopJ, Mauer M. Patternanalysis of human cutaneous mast cell populations by total body surface mapping. BJD. 2003 ; 148(2) : 224-8. https://doi.org/10.1046/j.1365-2133.2003.05090.x
  30. The Korean society of pathologists. Pathology 3rd ed. Seoul : Komoonsa. 1997 : 56, 159-60, 169, 222.
  31. Lawrence M Tierney, Stephen J McPhee, Maxine A Papadakis. Current Medical Diagnosis & Treatment. Seoul : Hanuri. 1999 : 846-74.
  32. Humbett M, Grant JA, Taborda-Barata L, Durham SR, Pfister R, Menz G. High-affinity IgE receptor-bearing cells inbronchial biopsies from atopic and nonatopic asthma. Am J Respir Crit Care Med. 1996 ; 153(6) : 1931-7. https://doi.org/10.1164/ajrccm.153.6.8665058
  33. Marshall JS. Mast-cell responses to pathogens. Nat Rev Immunol. 2004 ; 4(10) : 787-99. https://doi.org/10.1038/nri1460
  34. Abbs AK, Murphy KM, Sher A. Functional diversity of helper T lymphocytes. Nature. 1996 ; 383(6603) : 787-93. https://doi.org/10.1038/383787a0
  35. Borich L. Allergic rhinitis : systemic inflammation and implications for management. J Allergy Clin Immunol. 2003 ; 112(6) : 1021-31. https://doi.org/10.1016/j.jaci.2003.09.015
  36. Richard A. Goldsby, Thomas J. Kindt, Barbara A.Osborne, Janis Kuby. KUBY Immunology 5th ed. Seoul : World Science. 2006 : 104-5, 310, 327-30.
  37. Yun CS, Joo HA, Hwang CY. The Effects of Samulsopungeum and Prednisolone on NC/Nga Atopic Mice. J Korean Med Ophthalmol Otolaryngol Dermatol. 2010 ; 23(1) : 59-74.
  38. Pene J, Rousset F, Briere F et al. IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by Interferons gamma and alpha and prostaglandin E2. Proc. Natl. Acad. Sci. USA. 1988 ; 85(18) : 6880-4. https://doi.org/10.1073/pnas.85.18.6880
  39. Lee EH, Lee SE, Lee SH. Updates on the diagnosis and treatment of Atopic dermatitis. J Skin Bar Res. 2010 ; 12(1) : 103-10.

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