• Title/Summary/Keyword: s I

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Generation of a monoclonal antibody against AgI/II, a cellular surface protein of Streptococcus mutans GS5 (Streptococcus mutans GS5의 세포막 단백질 Ag I/II에 대한 단항체의 생산)

  • Cheon, Cheol-Wan;Baik, Byeong-Ju;Yang, Yeon-Mi;Han, Ji-Hye;Kim, Jae-Gon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.4
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    • pp.587-596
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    • 2006
  • Most of oral streptococci express the Antigen I/II (AgI/II) proteins, cell wall anchored adhesions. AgI/II protein binds to salivary agglutinin glycoprotein, a component of tooth pellicle and to ligands in other bacteria. These associations play important roles in bacterial colonization. Recently, it was reported that diverse host molecules also interact with AgI/II protein and that these interactions induce inflammatory responses from host cells. Among mutans streptococci containing -type hemolytic activity, Streptococcus mutans is a causative agent for dental caries. Compared with many other strains of S. mutans, GS-5 strain is unique in that this bacterium expresses truncated secretory AgI/II protein due to the nonsense mutation in the agI/II gene. This indicates that S. mutans GS-5 has a different clinical role and a recent report supported this idea based on the results from clinically isolated S. mutans strains. Previously, we had cloned agI/II gene from S. mutans GS-5 and generated recombinant N-terminal AgI/II protein. In this study, we further produced a hybridoma line expressing anti-AgI/II monoclonal antibodies named as 1C11A. This antibody showed high sensitivity to AgI/II protein in Western blot and ELISA. This new reagent will provide a basis for investigating the mechanisms of AgI/II-related diseases.

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A Nursing College Women's Meaning of Labor and Delivery : Phenomenological Method (간호학 전공 여대생이 인지한 분만의 의미에 대한 연구)

  • Yeo, Jung-Hee
    • Women's Health Nursing
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    • v.7 no.2
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    • pp.203-211
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    • 2001
  • A women's meaning about labor and delivery may influence the process of childbirth and performance of the maternal role. Therefore, negative meaning about the birth experience may have a negative impact on the childrearing. The purpose of this study is to explore the meaning of labor and delivery for the strategies of nursing intervention in positive childbirth experience. The data were collected through the in-depth interviews of 10 nursing college women on Jeju Island from October 2000 to January 2001. The interviews were conducted by investigator in the class room after school hours. Each interview lasted for about 30 minutes on average. Subjects were interviewed one at a time. The interviews were recorded with the consent of the subject. The data were analyzed by means of Giorgi's phenomenological analysis methods and catagorized according to the similarities of its contents. The investigator read the data repeatedly to identify the themes and the main meanings. Eight main meanings were identified as follows : 1)pain 2) shame and disgust 3)load 4)naturality 5)unreality 6)happiness 7)anxiety 8)maturity. Under these main meanings there were seventeen themes. I. Pain : (a) It's too painful (b) I don't want to deliver II. Shame and Disgust : (a) I am shameful (b) I am hateful III. Load : (a) I don't feel free (b) I have responsibility IV. Naturality : (a) It's in order to obtain a child (b) It's natural for women (c) It's a destiny of women V. Unreality : I don't feel real VI. Happiness : (a) I am glad (b) I am happy (c) It's miraculous VII. Anxiety : (a) I am anxious (b) I am worried VIII. Maturity : (a) I understand parents (b) I feel great. The results of the study will provide basic data for positive childbirth experience.

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PCR and Restriction Fragment Pattern of 16S rRNA gene of Vibrio vulnificus (Vibrio vulnificus ATCC 27562의 16S rRNA 유전자의 PCR과 제한효소절단 방식)

  • 허문수;정초록
    • Journal of Life Science
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    • v.8 no.2
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    • pp.126-130
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    • 1998
  • A pair of designed primers (sequences from Gene Bank) amplified 16S fRNA gene of V. vulnificus within polymerase chain reaction (PCR) machine. This PCR product is about 1.3kb DNA fragment. Six enzymes (BamH I, Alu I, Sau3A I, Hind III, Sal I, Sma I) were used for restriction pattern analysis of amplified 16S rRNA gene of V. vulnificus ATCC 27562. Digested fragments are resolved by 3% agarose gel. BamH I did not show digested fragment so, there was no cutting site of BamH I in PCR product. Alu I produced three small fragments from 400 bp to 200 bp. Sau3A I produced three fragments larger than Alu I from 70 bp and 500 bp. One of fragments of Sal I was same with 500 bp of Hind III fragment and the other was 750 bp. Sma I showed two fragments of 800 bp and 470 bp. The profile of digested fragments of 16S rRNA of V.vulnificus ATCC 27562 will may be able to use standard profile for identification of V. vulnificus.

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SOME RESULTS ON S-ACCR PAIRS

  • Hamed, Ahmed;Malek, Achraf
    • Communications of the Korean Mathematical Society
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    • v.37 no.2
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    • pp.337-345
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    • 2022
  • Let R ⊆ T be an extension of a commutative ring and S ⊆ R a multiplicative subset. We say that (R, T) is an S-accr (a commutative ring R is said to be S-accr if every ascending chain of residuals of the form (I : B) ⊆ (I : B2) ⊆ (I : B3) ⊆ ⋯ is S-stationary, where I is an ideal of R and B is a finitely generated ideal of R) pair if every ring A with R ⊆ A ⊆ T satisfies S-accr. Using this concept, we give an S-version of several different known results.

Preponderant Occurrence of sl natural methane hydrates: Spectroscopic analysis of crystalline structure transition (sI 천연 메탄하이드레이드의 존재 : 결정구조 변화의 분광학적 분석)

  • Yeon, Sun-Hwa;Seol, Ji-Woong;Lee, Huen
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.06a
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    • pp.387-390
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    • 2006
  • We report here that under strong attacksof external $CH_4$ guest molecules the sII and sH methane hydrates are structurally transformed to the crystalline me framework of sI, leading to favorable change of the lattice dimension of the host-guest networks. The High Power Decoupling $^{13}C$ NMR and Raman spectroscopies were used to identify structure transitions of the mixed $CH_4+C_2H_6$ hydrates (sIIl) and hydrocarbons (methylcyclohexane, isopentane) + $CH_4$ hydrates (sH). The resulting spectra indicate that most of the synthesized sII and sH hydrates were transformed to methane hydrate of sl under 110 bar and particularly the coexistence of sl with sII or sH appear according to the surrounding methane-rich gas conditions. The present findings might be expected to Provide rational evidences regarding the preponderant occurrence of naturally-occurring sI methane hydrates in marine sediments.

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Rheological Properties of the Solutions of Incompatible Polymer Blends

  • Sohn, Jeong-In;Ree, Taik-Yue
    • Bulletin of the Korean Chemical Society
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    • v.2 no.4
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    • pp.142-147
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    • 1981
  • A blend polymeric system composed of poly(methyl methacrylate) (PMMA or PM) and polystyrene (PS) dissolved in chloroform was rheologically studied. The viscosities ${\eta}_{bl}$ of the blend system with various blending ratios ${\chi}$ changing from zero (pure PS solution) to unity (pure PMMA solution) were measured at $25{\circ}C$ as a function of shear rates ${\dot{s}}$ by using a Couette type viscometer. ${\eta}_{bl}$ at a given ${\dot{s}}$ decreased exponentially with ${\chi}$ reaching asymptotic constant value of ${\eta}_{bl}$ ; ${\eta}_{bl}$ at a given ${\chi}$ is greater at a smaller ${\dot{s}}$. These results are explained by using Ree-Erying's theory of viscosity, ${\eta}_{bl}=(x_1{\beta}_1/{\alpha}_1)_{b}_1+ (x_2{\beta}_2/{\alpha}_2)_{bl}[sinh^{-1}{\beta}_2(bl) {\dot{s}}]/{\beta}_2(bl){\dot{s}}$. The Gibbs activation energy ${\Delta}G_i^\neq$(i = 2 for non-Newtonian units) entering into the intrinsic relaxation time ${\beta}$ is represented by a linear combination ${\Delta}G_i^\neq(bl) ={\chi}{\Delta}G_i^{\neq}_{iPM}+(1-{\chi}){\Delta}G_i^{\neq}_{iPS}$;the intrinsic shear modulus$[[\alpha}_i]^{-1}$ is also represented by $[{\alpha}_i(bl)]^{-1}={\chi}[{\alpha}_{iPM}]^{-1}+(1-{\chi})[{\alpha}_{iPS}]^{-1}$ and the fraction of area on a shear surface occupied by the ith flow units $x_i(bl)$ is similarly represented, i.e., $x_i(bl) = {\chi}x_{iPM}+(1-{\chi})x_{iPS}$. By using these ideas the Ree-Eyring equation was rewritten which explained the experimental results satisfactorily.

Reduction of Nitro and Nitroso Compounds by Glucose and Baker's Yeast (Glucose와 Baker's Yeast에 의한 니트로와 니트로소 화합물의 환원반응)

  • 김경순;박종옥류병호최희숙
    • KSBB Journal
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    • v.11 no.6
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    • pp.623-629
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    • 1996
  • m-Bromonitrobenzene was reduced to m-bromoaniline with a 86∼91% yield by either baker's yeast (Sigma type I) or glucose and baker's yeast in conjunction with glucose. Nitrosobenzene was reduced to aniline in good yields using baker's yeast(Sigma type I) only.

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Taxonomic Studies on Genus Incilaria ( Pulmonata , Philomycidae ) in Korea (한국산 민달팽이속 ( Incilaria ) 육산패류의 분류학적 연구)

  • 이준상;권오길
    • The Korean Journal of Malacology
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    • v.13 no.1
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    • pp.29-36
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    • 1997
  • Incilaria속 육삼패류의 분류를 위해 국재 7지역에서 채집된 100개체를 대상으로 수평전분전기 영동법을 이용한 동위효소 분석을 실시하였다. I. fruhstorferi와 I. bilineata 종내 집단의 유전적 근연치는 S=0.635와 S=0.482의 유전적 근연치를 나타내는 Incilaria sp. group이 형성되어 이들의 분류학적 위치에 대한 추가 연구가 요구되었다. 각 종간 유전적 근연치는 I. fruhstorferi와 I. bilineata 간에는 S=0.364이며, I. fruhstorferi와 Incilaria sp.는 S=0.427, I. bilineata와 Incilaria sp.는 S=0.3999로 나타나 Incilaria sp. group은 I. bilineata보다 I. fruhstorferi에 가까운 유전적 유연관계를 나타냈다.

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국제양돈수의학회(I.P.V.S.) -수의업무 세계화의 길이 보인다.-

  • 강영배
    • Journal of the korean veterinary medical association
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    • v.32 no.8
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    • pp.535-539
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    • 1996
  • 국제양돈수의학회(International Pig Veterinary Society ; I.P.V.S) 제14차 총회 및 학술대회가 1996년 7월 7일부터 11일까지 이태리 중북부의 대학도시 볼로냐(Bologna, Italy)에서 개최되었다. I.P.V.S.는 1969년에 영국의 캠브리지 (Cambridge, United Kingdom)에서 창립된 이래 1996년 현재 53개 회원국 1,700 여명의 회원을 확보하고 있으며, 세계 각국의 양돈과 수의학에 관심을 가지고 있는 학자, 생산자 그리고 관련업계 인사들의 학술$\cdot$기술 정보교류와 협력을 목적으로 매 2년마다 세계 주요도시를 순회 하면서 개최되고 있다. 앞으로 2년후(1998년도)에는 I.P.V.S.의 탄생국인 영국의 버밍햄(Birmingham, United Kingdom)에서 제 15차 총회 및 학술대회를 개최할 예정이며 2000년대에는 시드니 올림픽과 때를 맞추어 호주의 멜보른(Melboume, Australia)에서 제16차 총회를 개최하기로 결정되어 있다. 금년(1996년도) I.P.V.S 총회에는 우리나라에서 30개 기관/업체로부터 50여명이 참여한 바 있으며 총 27편 (구두 9편, 포스터 18편)의 학술논문을 발표하는 등 눈부신 활약을 보인 바 있다. 금년도 I.P.V.S. 총회의 특징은 돼지의 전염성 질병중 근래에 우리나라에서도 문제시 되고 있는 '돼지 생식기 호흡기 중후군 (Porcine Reproductive and Respiratory Syndrome ; PRRS)'이 세계적인 관심의 대상이었으며, 이태리 양돈병리축산학회 (Societa Italiana di Patologia ed Allevamento dei Suini ; S.I.P.A.S) 주관으로 '돼지 적리 (Swine Dysentery)'에 관한 Satellite Symposium이 개최되었다는 점이다. WTO 이래 어려워지고만 있는 우리나라 축산업 분야중 그래도 비젼이 보이는 양돈산업과 관련하여, I.P.V.S.를 통하여 우리나라 수의업무의 세계화의 길을 찾아보고자, 1 국제양돈수의학회(I.P.V.S)의 역사 (양돈과 수의업무 전문가의 새로운 만남의 장), 2 I.P.V.S.에의 참여(제13차 방콕대회), 3 한국 수의사의 활약(제14차 볼로냐대회), 4 세계화의 길(제15차 버밍햄대회, 제16차 멜보른대회), 5 국내양돈의 문제와 우리가 해야할 일(수의업무 세계화의 길을 찾자)등의 순으로 적어보고자 한다.

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