• 한국미생물·생명공학회지
• /
• 제44권2호
• /
• pp.163-170
• /
• 2016

전국 각지에서 수집된 발표식품으로부터 젖산균을 분리하고 유해균인 B. cereus, L. monocytogenes, S. Typhimurium, S. aureus 및 S. epidermidis 균주에 대하여 생육 억제효능이 강한 L2167 균주를 선발하고, 16S rDNA 분석법을 이용하여 L. plantarum로 동정하였다. L2167 균주의 단백질 분획물을 제작하여 S. aureus 등 5종의 유해균 배양액에 첨가한 후, 전자현미경으로 관찰하였다. L. plantarum L2167 균주의 균체 단백질 분획물은 유해균의 생육을 억제하였으며, 생육 억제활성은 균체 내부에 함유된 단백질이 유해균의 세포막을 파괴하는 것에서 기인하는 것으로 추정되었다. L. plantarum L2167은 pH 5.5로 조절한 MRS 배지에서 35℃로 배양하였을 때, 가장 우수한 비성장속도를 나타냈으며, 대조구로 사용된 L. plantarum KCTC21004보다 NaCl에 대하여 민감한 특징을 나타내었다. 본 연구를 통하여 선발한 L. plantarum L2167 균주는 추가연구를 통하여 유해균의 생육을 효율적으로 억제할 수 있는 스타터 균주로서 개발될 수 있을 것으로 기대된다.

• 한국전기전자재료학회논문지
• /
• 제33권3호
• /
• pp.219-224
• /
• 2020

Al₂O₃ powders with particle sizes of 0.35 ㎛, 0.5 ㎛, 1.5 ㎛, and 2.5 ㎛ are deposited onto glass and Cu substrates using the aerosol deposition (AD) process. The deposition characteristics of Al₂O₃ films using those four types of Al₂O₃ powders are investigated to determine the influence of the particle size on the films. To observe detailed micro-structures of the films, the cross-section and surface morphology are observed. Then, the crystalline size and internal strain are calculated from X-ray diffraction peaks in order to confirm the hammering effect as well as the micro-strain during the AD deposition. From the above results, deposition mechanisms related to the particle size are studied. The results of this study indicate the optimal particle size and formation mechanisms for dense Al₂O₃ film with a smooth surface roughness as well as for a porous Al₂O₃ film with a rough surface roughness.

• 대한미생물학회지
• /
• 제15권1호
• /
• pp.9-17
• /
• 1980

In the ecology and epidemiologic studies on various serotypes of atypical mycobacteria(AM), Schaefer's bacterial agglutination test(BA) provided the basis of the serologic procedures. Recently, attempts have been made to modify and to simplify the Schaefer's BA such as a slide agglutination test(Engel & Beerwald, 1970), a "simplified" BA(Reznikov & Leggo, 1972), an agglutination inhibition test(Richards & Eacret, 1972) and "micromethod"(Thoen et al., 1975). The BA, however, was not widely applied as a routine laboratory test mainly because it requires much times and labors to perform and partley because it is not applicable to hydrophobic strains either often encountered in the isolation of AM in the clinical bacteriology or stock strains maintained in the laboratory. On the contrary, fluorescent antibody technique with mycobacteria may have advantages over the BA because it is far more simpler in serologic procedures and is applicable to all strains of mycobacteria regardless of smooth or rough types of cultures. At the present, it is well known that the type-specific antigens are lacking on the surface of rough type of AM compared to that on smooth type of strain, but the antigenicity on the surface of the hydrophobic strains of AM which resulted from a series of subculture and the strain in the laboratory for 3 to 6 months has not been clarified. In this study, an attempt to serotype the hydrophobic strains of M. scrofulaceum serotype 41, 42 and 43 by fluorescent anti-complement(FAC) technique was made. The FAC technique with mycobacteria was also described in detail. In the summary, the complement fixing antibody titres of reference sera to smooth types of homologous serotype was highest, but the antibody titres of reference sera to hydrophobic strains of serotypes, 41, 42 and 43 gave two-to 8-folds lower than those to smooth type of strains. Although the sensitivity of type-specific antigens on the hydrophobic strains to reference sera was much lower, using the two units of reference sera determined by titration with hydrophobic strains, three serotypes, i. e., 41, 42 and 43 were specifically differentiated one another by FAC technique. This result indicated that the hydrophobic strains which were maintained in the laboratory at least for 6 months still retain type-specific antigen detectable by FAC technique.

• 한국건축시공학회지
• /
• 제19권3호
• /
• pp.201-207
• /
• 2019

본 연구에서는 변형속도에 따른 비정질 강섬유보강 시멘트복합체의 직접인장특성에 대하여 평가하였다. 길이 15, 30mm의 박판형 비정질 강섬유를 각각 1.0, 1.5, 2.0% 혼입한 섬유보강 시멘트복합체를 제작하였으며, 변형속도 $10^{-6}/s$(정적), $10^1/s$(동적)의 조건에서 직접인장시험을 수행하였다. 그 결과, 길이 15mm의 비정질 강섬유는 섬유의 섬유의 길이가 짧고 혼입개체수가 많기 때문에 섬유가 매트릭스로부터 인발되었다. 반면, 길이 30mm의 비정질 강섬유는 섬유의 표면이 거칠고 비표면적이 크기 때문에 매트릭스와의 부착성능이 우수하지만, 박판형의 섬유 형상이 전단력에 약하기 때문에 섬유가 인발되지 않고 파단 되었다. 섬유의 길이가 길수록 인장강도, 변형능력 및 인성이 큰 것으로 나타났다. 반면, 길이 30mm의 비정질 강섬유는 매트릭스로부터 인발되지 않고 파단 되지만 길이 15mm의 비정질 강섬유는 매트릭스로부터 인발되기 때문에 변형속도의 영향을 받는 섬유-매트릭스 계면의 부착효율이 크게 되어, 인장강도, 변형능력 및 인성에 대한 동적증가계수가 큰 것으로 나타났다.

• Fibers and Polymers
• /
• 제7권4호
• /
• pp.372-379
• /
• 2006

The surface topography, tensile properties, and thermal properties of ramie fibers were investigated as reinforcement for fully biodegradable and environmental-friendly 'green' composites. SEM micrographs of a longitudinal and cross sectional view of a single ramie fiber showed a fibrillar structure and rough surface with irregular cross-section, which is considered to provide good interfacial adhesion with polymer resin in composites. An average tensile strength, Young's modulus, and fracture strain of ramie fibers were measured to be 627 MPa, 31.8 GPa, and 2.7 %, respectively. The specific tensile properties of the ramie fiber calculated per unit density were found to be comparable to those of E-glass fibers. Ramie fibers exhibited good thermal stability after aging up to $160^{\circ}C$ with no decrease in tensile strength or Young's modulus. However, at temperatures higher than $160^{\circ}C$ the tensile strength decreased significantly and its fracture behavior was also affected. The moisture content of the ramie fiber was 9.9 %. These properties make ramie fibers suitable as reinforcement for 'green' composites. Also, the green composites can be fabricated at temperatures up to $160^{\circ}C$ without reducing the fiber properties.

• 한국윤활학회:학술대회논문집
• /
• 한국윤활학회 1999년도 제29회 춘계학술대회
• /
• pp.67-72
• /
• 1999

Scanning probe Microscope(SPM) such as Scanning Tunneling Microscope(STM) and Atomic Force Microscope(AFM) was shown to be the powerful tool for nano-scale characterization of material surfaces Using this technique, surface morphology of the cyclically deformed Cu or Cu-Al single crystal was observed. The surface became proportionately rough as the number of cycles increased, but after some number of cycles no further change was observed. Slip steps with the heights of 100 to 200 nm and the widths of 1000 to 2000 nm were prevailing at the stage. The slipped distance of one slip system at the surface was not uniform. and formation of the extrusions or intrusions was assumed to occur such place. By comparing the morphological change caused by crystallographic orientation, strain amplitude, number of cycles or stacking fault energy, some interesting results which help to clarify the basic mechanism of fatigue damage were obtained. Furthermore, applicability of the scanning tunneling microscopy to fatigue damage is discussed.

• 한국지반공학회지:지반
• /
• 제10권1호
• /
• pp.103-118
• /
• 1994

세주응력을 서로 독립적으로 조절할 수 있는 입방체형삼축시험기를 이용하여 모래시료에 대한 일련의 배수삼축시험이 실시되었다. 그리고 Lade에 의해 제안된 등방단일경화구성모델을 사용하여 입방체형삼축시험시와 동일한 응력경로에 대한 응력-변형률 거동을 해석하였다. 등방단일경화구성모델에 의한 거동 예측치는 초기재하단계(primary loading)시 입방체형삼축시험결과와 좋은 일치를 보이고 있는 것으로 나타났다. 그러나 반복재하단계(unloading과 reloading)의 경우 거동예측치는 시험결과와 많은 차이를 보이고 있다. 이것은 등방단일경화구성모델의 소성일경화계수가 시험결과의 경화부분을 근거로 하여 결정되었기 때문에 연화부분에서 많은 오차가 있는 것으로 판단된다.

• 치과방사선
• /
• 제24권2호
• /
• pp.403-410
• /
• 1994

The purpose of this study was to investigate the irradiation effects on the secretory granules of the rat submandibular gland acinar cell. For this study, Sprague-Dawley strain rats were irradiated to their neck region with the dose of 5Gy and l0Gy by 6MV X-radiation, and sacrificed on the experimental periods after irradiation. The authors observed the ultrastructural changes of the secretory granules of the rat submandibular gland acinar cell under a trasmission electron microscope. The results were as follows: In the transmission electron microscopic examination, secretory granules were blurred in limiting membrane on the 3hours groups after irradiation. And they showed decrease in number, irregularities in shape and distributional pattern, and inhomogeneous internal electron density on the Iday and 3days groups. After then, these changes were recovered in shape and distributional pattern on the 14days groups, and changes of internal electron density and limiting membrane were recovered on the 28days groups after irradiation. Among the intracellular organelles, rough endoplasmic reticulum was scattered, but golgi complex was not changed. And such pathologic changes were earlier and more prominent in 10Gy irradiated groups than in 5Gy irradiated groups.

• 열처리공학회지
• /
• 제11권4호
• /
• pp.274-282
• /
• 1998

The effect of Al addition on the fatigue properties of austenitic Fe-25Mn-Al-0.5C steels was studied. When Al was not added to the Fe-25Mn 0.5C steel, the strain induced ${\varepsilon}$ martensites, deformation twins and slip bands were formed during fatigue deformation. When 2wt% of Al was added to the steel, the deformation twins and slip bands were formed during fatigue deformation. When 5wt% of Al was added, only slip bands were formed. In low cycle fatigue test, the alloys containing 0wt% and 2wt%Al showed the cyclic hardening due to ${\varepsilon}$ martensites and deformation twins, resulting in shorter fatigue lives than the alloy containing 5wt%Al. In fatigue crack propagation test, the alloy without Al showed the highest crack propagation rate. The fracture surface of the alloy without Al was flat, whereas that of the alloy with 2% or 5%Al was rough. The ${\Delta}K_{th}$, values of the alloys with 0%, 2% and 5%Al were 16, 17.5, and $20.5MPam^{1/2}$, respectively.

• 한국식품영양과학회지
• /
• 제26권6호
• /
• pp.1077-1085
• /
• 1997

This study was carried out to elucidate the utilization of the fusant for shortening the ripening time by making an observation of the microstructure and the profile of component change. In ripening cheese, moisture content of the sample treated with tested strain is not a remarkable difference among the test samples. With an increase of the ripening time, L. helveticus showed the highest increase in protein content, followed by fusant, and then L. bulgaricus. The fat content of all starters was gradually decreased while it was it was rapidly decreased after 7 days. The pH of all starters was gradually decreased when the ripening time increased. The titratable acidity was greatly increased between a 9th day and a 15th day ripening. In investigating the light microscopic microstructure of ripened cheese samples, the sample treated with fusant indicated little difference from the other starters in decomposition of protein and fat components by microbial enzymes. In SEM observation, the structure of all cheese samples was uniform and the rough texture was converted into smooth texture by the interaction of cheese components and the abscission of single bond in casein matrix when the ripening time is increased. The fusant showed similar results in the examination of component change and its microstructure compared with the other starters. Therefore, it was revealed that the fusant can be partially used as a cheese starter instead of conventional starters by replacing them or combining them together with the other starters for shortening the ripening time.