• KSBB Journal
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• 제8권1호
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• pp.42-48
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• 1993

Continuous degradation of formaldehyde by using a rotating disc contactor was investigated in this study. Peudomonas putida H-5K was selected as a mutant using N-methyl N'-nitro N-nitrosoguandine (250$\mu\textrm{g}$/$m\ell$), which showed 1.5 times higher ability of formaldehyde degradation than that of the parent strain. Enzyme activity for formaldehyde degradation released form Peudomonas putida H-5K showed the highest level of 6.2mo1/min/mg protein in the 2% glucose mineral medium containing 0.02% formaldehyde. Degradation of formaldehyde from the first stage in rotating disc contactor was 95% and 5% from the 4th stage when the reactor was fed with 0.02% or 0.04% formaldehyde solution at a rate of 20$m\ell$ per hour. Continuous degradation of formaldehyde using rotating disc contactor was above 95%o in the medium containing 0.04% formalchyde, at the medium feed ratc of 20$m\ell$ per hour.

• 한국환경보건학회지
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• 제21권4호
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• pp.49-55
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• 1995

Dyes are released into the environment as industrial wasterwater. Dyes are considered to be a pollution problem because of the wide spread into environment with a variety of colors. Continuous biodegradation of reactive dyes such as Rifacion Red H-3EB, Rifazol Blue BT, Rifacion Yellow P4G and Rifacion Brown RT were demonstrated using multistage rotating disc contactor immobilized by Aspergillus sojae B-10. Aspergillus sojae B-10 was cultivated the optimal medium containing 2.0% glucose, 0.08% $NaNO_3$, 0.1% $KH_2PO_4$ and 0.5% $MgSO_4\cdot 7H_2O$, pH 5 at 32$\circ$C. Mycelium of Aspergillus sojae B-10 were guck to the rotating disc for 10 days until steady state. For continuous biodegradation of reactive dyes by using rotating disc contactor, it was most effective biodegradation in the medium containing 1,000 ppm each dyes at the medium feeding rate of 20 ml per hour. Under the conditions biodegradation of each dyes on 2, 4 and 6 days were 20~50%, 75% and 96%, respectively. Therefore, practical application of reactive dyes were carried out at the feeding rate of 20 ml/h as synthetic wasterwater containing 500 ppm of mixture reactive dyes. It was found the highest levels of 94% biodegradation during 20 days.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권4호
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• pp.309-312
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• 2004

Aspergillus sojae B-l0 was immobilized and used to treat model dye compounds. The model wastewater, containing 10 ppm of azo dyes such as Amaranth, Sudan III, and Congo Red, was treated with cells attached to a rotating disc contactor (RDC). Amaranth was decolorized more easily than were Sudan III and Congo Red. Decolorization of Amaranth began within a day, and the dye was completely decolorized within 5 days of incubation. Both Sudan III and Congo Red were almost completely decolorized after 5 days of incubation. Semicontinuous decolorization of azo by reusing attached mycelia resulted in almost complete decolorization in 20 days. This experiment indicated that decolorization was successfully conducted by removing azo dyes with Aspergillus sojae B-10.

• 생명과학회지
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• 제7권2호
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• pp.112-118
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• 1997

Gallic acid로부터 pyrogallol로 전환하는 균주를 토양에서 분리 동정하여 얻는 Pseudomonas sp. KS-96의 균체를 회전원판 반응조에 고정화시킨 후 연속전환을 시도하여 다음과 같은 결과를 얻었다. Gallate decarboxylase의 활성은 배양 24시간에서 가장 좋았다. 반응조의 원판에 gallic acid가 함유된 액체 배양액을 시간당 20m${\ell}$씩 공급하면서 균체막의 두께가 steady state에 도달할때까지 공급하였다. 균체를 고정화 시킨 후 시간 경과에 따른 전환율은 18시간부터 36시간에서 전환율이 높았다. 균체 고정화 후에 gallic acid를 15g/${\ell}$ 함유하는 기질을 시간당 30m${\ell}$씩 주입했을 때 pyrogallol의 전환율이 가장 좋았으며 회전원판 반응조에 의한 연속적 전환은 6일에서 8일까지가 각각 81%, 80%로서 가장 높았다.

• 한국물환경학회지
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• 제20권2호
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• pp.190-195
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• 2004

A new technology for advanced wastewater treatment was developed using a modified Rotating Biological Contactor (RBC) process, named as Rotating Activated Bacillus Contactor (RABC) process that utilizes Bacillus sp., the facultatively anaerobic or activated microaerophilic bacteria on multiple-stage reticular rotating carriers, as a predominant species. The RABC process for a municipal wastewater with relatively low concentrations of organics, nitrogen, and phosphorus showed stable and high removal efficiencies, less than $BOD_5$ 10 mg/L, T-N 15 mg/L, and T-P 1.5 mg/L in final effluent. The performance load of RABC process was shown to be $1.23kg{\cdot}BOD/m^2{\cdot}day$ for the first stage (average $0.31kg{\cdot}BOD/m^2{\cdot}day$ for the total stages) based on both removed BOD and converted disc area corresponding to the reticular one. The sludge produced in the RABC process is characterized by low generation rate (about $0.18kg{\cdot}MLSS/kg{\cdot}BOD$) and excellent settleability. The number ratio of Bacillus ($2.4{\times}10^6CFU/ml$) to heterotrophic bacteria ($3.6{\times}10^7CFU/ml$) inhabiting in the biofilms of the RABC process was 6.7 %, indicating that Bacillus sp. was a predominant species in the biofilms. The RABC process with reticular rotating carriers showed its excellent performance for the advanced wastewater treatment without any offensive odor problem due to organic overloading.

• 대한토목학회논문집
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• 제4권3호
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• pp.1-9
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• 1984

본(本) 연구(硏究)는 고정미생물막(固定微生物膜)을 이용한 회전원판법(回轉圓板法)이 저농도(低濃度)의 유입수(流入水)로부터 고농도(高濃度) 유입수(流入水)로 단계별(段階別)로 변(變)하는 오수(汚水)에 대해 각(各) 단계별(段階別) 처리효율(處理効率)과 회전원판(回轉圓板)의 회전속도(回轉速度)와 체류시간(滯留時間)이 처리효율(處理効率)에 어떤 영향을 미치는지를 분석(分析)하여 국내오수처리(國內汚水處理)에 대한 회전원판법(回轉圓板法)이 개발(開發)에 기여코자 한다. 고농도(高濃度)의 유입수(流入水)는 저농도(低濃度)의 일반오수(一般汚水)에 분뇨(糞尿)를 투입(投入)하여 그 농도(濃度)를 인위적(人爲的)으로 만들어 사용했으며, 이들 유입수(流入水)에 대한 pilot plant의 실험결과(實驗結果)는 원판(圓板)의 회전속도(回轉速度)가 3 rpm, 체류시간(滯留時間)이 120 분(分)인 경우가 경제성(經濟性)이 있는 것으로 나타났으며, 단계별(段階別) 처리효율(處理効率)은 첫 단계(段階)에서 유입수(流入水)의 농도(濃度) 관계(關係)없이 40~50%정도의 $BOD_5$ 제거율(除去率)을 나타내었고, 용적부하(容積負荷) $2.0kgBOD/m^2$ 일(日)이 고농도(高濃度)에서도 방류수(放流水)의 $BOD_5$ 제거율(除去率)이 88~90% 정도로 양호(良好)하였다.

• 한국환경농학회지
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• 제4권1호
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• pp.37-42
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• 1985

최근(最近) 오(汚)${\cdot}$폐수처리(廢水處理)에 있어서 고정생물막(固定生物膜)을 이용(利用)한 방법(方法)이, 처리장(處理場) 운전(運轉)에 요구(要求)되는 동력(動力) 및 인력(人力)의 절감(節減)이라는 장점(長點)으로 해서, 활발(活發)히 추진(推進)되어 오고 있으며 또한 국내(國內)에서도, 이를 적용(適用)한 처리장(處理場)이 점차 증가(增加)하고 있다. 본(本) 연구(硏究)는 고정생물막법(固定生物膜法)의 하나인 RBC(roating biological contactor; 회전생물막(回轉生物膜) 접촉체(接觸體))에 의(依)한 오수처리방법(汚水處理方法)을 실규모(實規模)의 pilot plant를 현장(現場)에 설치(設置), 5개월(個月) 간(間)의 운전(運轉)에 의(依)해 얻어진 결과(結果)를 분석(分析), 1984. 5. 16 환경청(環境廳)에서 고시(告示)(제(第)$84{\sim}8$호(號))한 오수정화시설중(汚水淨化施設中), 회전원판(回轉圓板) 접촉방법(接觸方法)에서 정(定)하는 설나기준(設羅基準)을 실제(實際) 처리장(處理場)에 어떻게 적용(適用)할 것인지에 대(對)한 검토(檢討)를 해보기 위(爲)해서였다. 회전판(回轉板)의 회전속도(回轉速度)와 체류시간(滯留時間)은 오수(汚水)의 BOD제거효율(除去效率)에 큰 상관관계(相關關係)를 가지며 회전판(回轉板)의 원주달도(圓周達度)가 $18{\sim}20m$/분(分)인 경우가 경제성(經濟性)이 있는 것으로 나타났으며 일반(一般) 가정하수(家庭下水)에 대(對)한 RBC조내(槽內)의 체류시간(滯留時間)은 120분(分)(2시(時) 간(間))정도(程度)에서도 BOD제거율(除去率)이 90% 이상(以上)이 됨을 알 수 있었다. 그리고 고농도(高濃度) 오빙(汚氷)(BOD $250{\sim}400mg/l$)인데 대(對)해서도 제거율(除去率) $85{\sim}90%$정도(程度)로 양호하였다.

• 한국양식학회지
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• 제16권3호
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• pp.142-150
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• 2003

Performance of a biological filter, the rotating biological contactor (RBC), is affected by rotational speed and hydraulic residence time (HRT). A RBC with a disc diameter of 62 cm, total surface area of 48.28 $m^2$, volume of 0.34 ㎥, and submergence ratio of 35.4% was tested for the combinations of five rotational speeds (1, 2, 3, 4 & 5 rpm) and three HRT (0.5, 1.0 & 2.0 hr) to find out the maximum removal efficiencies of total ammonia nitrogen (TAN) and nitrite nitrogen of a simulated recirculating aquaculture system. Ammonia loading rate in the system was 25 g of TAN/ ㎥. day. Removal efficiencies were checked when TAN concentrations in the system stabilized for 3 days in each treatment. The concentration of TAN in the system decreased with increasing rotational speed of the RBC up to 4 rpm in all HRT (P<0.05). At the rotational speed of 5 rpm, the efficiencies decreased in all HRT (P<0.05). When the rotational speeds were 1, 2, 3, 4, and 5 rpm, TAN concentrations in the system were 1.35, 0.94, 0.69, 0.66, and 0.76 mg/L at the 0.5 hr HRT, 2.86, 1.18, 0.96, 0.87, and 1.11 mg/L at the 1.0 hr HRT, and 5.30, 2.44, 1.99, 1.77, and 2.01 mg/L at the 2.0 hr HRT, respectively. The TAN removal efficiencies of the RBC at the rotational speeds of 1, 2, 3, 4, and 5 rpm were 32.9, 49.5, 65.1, 72.9, and 62.9% in 0.5 hr HRT,33.1, 74.1, 87.1, 95.8, and 78.5% in 1.0 hr HRT, and 35.5, 76.7, 89.6, 97.0, and 85.5% in 2.0 hr HRT, respectively. TAN removal efficiency of RBC per pass increased with increasing HRT. However, TAN concentration in the system also increased. The best operating condition among the treatments was obtained at the treatment of 0.5 hr HRT and 4 rpm (P<0.05). The TAN concentration was 0.66 mg/L. Concentrations of nitrite nitrogen (NO$_2$$^{[-10]}$ -N) in the system decreased with increasing rotational speed in all HRT while that in the system increased with increasing HRT in all rotational speeds. The ranges of NO$_2$$^{[-10]}$ -N concentrations at HRT of 0.5, 1.0, and 2.0 hr in the system were 0.26~0.32, 0.31~0.56, and 0.43~l.45 mg/L, respectively. The ranges of daily removal rates of TAN in this system were 20.03~23.0 g TAN/㎥ㆍday and those of nitrite nitrogen were 19.65~30.25 g NO$_2$$^{[-10]}$ -N/㎥ㆍday.