Continuous Degradation of Formaldehyde by Peudomonas putida H-5 Using Rotation Disc Contactor

회전원판반응조에 의한 Peudomonas putida H-5의 포름알데히드의 연속적 분해

Continuous degradation of formaldehyde by using a rotating disc contactor was investigated in this study. Peudomonas putida H-5K was selected as a mutant using N-methyl N'-nitro N-nitrosoguandine (250$\mu\textrm{g}$/$m\ell$), which showed 1.5 times higher ability of formaldehyde degradation than that of the parent strain. Enzyme activity for formaldehyde degradation released form Peudomonas putida H-5K showed the highest level of 6.2mo1/min/mg protein in the 2% glucose mineral medium containing 0.02% formaldehyde. Degradation of formaldehyde from the first stage in rotating disc contactor was 95% and 5% from the 4th stage when the reactor was fed with 0.02% or 0.04% formaldehyde solution at a rate of 20$m\ell$ per hour. Continuous degradation of formaldehyde using rotating disc contactor was above 95%o in the medium containing 0.04% formalchyde, at the medium feed ratc of 20$m\ell$ per hour.