• Journal of Biosystems Engineering
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• v.2 no.1
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• pp.7-24
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• 1977

This paper is the forth and fifth one of the study on balanced type oscillating mole drainer. In the light of the results from previous reports about the model tests, some design criteria were established and a prototype machine was set up for experimental purpose. Motion characteristics and functionof the each parts of the machine were checked and analyzed. After that, performance tests of the prototype machine were carried out in thefield. Obtained results are summarized as follows ; 1. Ten centimeter of the bullet diameter was determined so as to be able to attach it to the tractors with capacity of 30 PS to 40 PS. 2. To maintain the balance between the moments of the front shank and rear shank, the oscillating amplitude of the rear bullet was determined to be larger than that of the front bullet. At the same time , the oscillating direction of the rear bullet was designed with the inclines of ten to thirty degrees. 3. An octagonal dynamo transduced was developed for measuring the compressive force of the upper link is measuring the draft force of the machine. Acceptable linear relationship between forces and strain responses from O.D.T. was obtained. 4. Analysing the balancing mechanism of the acting part of the machine , it was found that the total draft force of the machine was equal to the difference between the sum of the draft force produced from the right and left side bending moments of the lower drawber and the compressive force on the upper link. 5. There are acceptable linear relationship between the strain and twisting moment by driving shaft, and between strain and shank moment. Above results enable us to carry out the field experiment with prototype machine. 6. When the test machine was used in the field, it was possible to reduce the oscillating acceleration by forty percent in average as compared it with the single bullet mole drainer. 7. When the test machine was used under the oscillating condition, the dratt torce was reduced by 27 percent to 59 percent as compared it with the test machine under non-oscillating condition, while the draft force was increased by 7 percent to 20 percent as compared it with the mole drainer having oscillating single bullet. The reasoning behind this fact was considered as the resistance force due to the rear shank and bullet. 8. As the amplitude and frequency of the bullet were increased, the torque was increased accordingly. This tendency could be varied with the various characteristics of the given soils. And the larger frequency and amplitute, the more increasing oscil\ulcornerlating power but decreasing draft brce were needed, and draft force was increased as the velocity was increased.9. When the amplitude of the rear bullet was designed to be larger than that of the front bullet, the minimum value of the moment was lowered and oscillating acceleration was reduced. And when the oscillating direction of the rear bullet was declined back\ulcornerwards, oscillating acceleration was increased along with the increasing angle of decli\ulcornernation. When the test machine was operated in high speed, the difference between maximum moments and minimum ones became narrow. This varying magnitude of moments appeared on the moment oscillogram seems to be correlated to the oscillating acceleration and draft force. 10. From the analysis of variance, it was found that those factors such as frequency, amplitude, and operating velocity significantly affected in the oscillating acceleration, the draft resistance, the torque, the moment, and the total power required. And interaction between frequency and amplitude affected in the oscillating acceleration. 11. Within the given situation of this study, the most preferable operating conditions of the test machine were 7 Hz in oscillating frequency, 0.54 m/sec in operating velocity, and 39.1 mm in oscillating amplitude of front and rear bullets. However, it is necessary to select the proper frequency and magnitude of oscillation depending on the soil properties of the field in which the mole drainer is practiced by use of a bal1nced type oscillating mole drainer. 12. It is recommended that a comparative study of the mole drainers would be performed in the near future using two separate balanced oscillating bullet with the one which is operated by oscillating the movable bullet in a single cylinder or other balanced type which may be single oscillating bullet with spring, damper or balancing weight, and that of thing. To expand the applicability of the balanced type oscillating mole drainer in practical use, it is suggested to develop a new mechanism which perform mole drain with vinyl pipe or filling material such as rice hull.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.450-456
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• 2016

Rhizoctonia leaf blight (large patch) has become a serious problem in Korean lawn grass, which is extremely hard to treat and develops mostly from the roots of lawn grass to wither it away. Rhizoctonia leaf blight (large patch) is caused by Rhizoctonia solani AG2-2 (IV). To develop zoysia japonica with strong disease tolerance against this pathogenic bacterium, ${\beta}-1,3-glucanase$ was cloned from zoysia japonica, which is one of the PR-Proteins known to play a critical role in plant defense reaction. ${\beta}-1,3-glucanase$ is known to be generated within the cells when plant tissues have a hypersensitive reaction due to virus or bacterium infection and secreted outside the cells to play mainly the function of resistance against pathogenic bacteria in the space between the cells. This study utilized the commonly preserved part in the sequence of corn, wheat, barley, and rice which had been researched for their disease tolerance among the ${\beta}-1,3-glucanase$ monocotyledonous plants. Based on the part, degenerate PCR was performed to find out the sequence and full-length cDNA was cloned. E.coli over-expression was conducted in this study to mass purify target protein and implement in vitro activation measurement and antibacterial test. In addition, to interpret the functions of ZjGlu1 gene, each gene-incorporating plant transformation vectors were produced to make lawn grass transformant. Based on ZjGlu1 protein, antibacterial activity test was conducted on 9 strains. As a result, R. cerealis, F. culmorum, R.solani AG-1 (1B), and T. atroviride were found to have antibacterial activity. The gene-specific expression amount in each organ showed no huge difference in the organs based upon the transformant and against 18s gene expression amount.

• The Korean Journal of Mycology
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• v.17 no.1
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• pp.1-6
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• 1989

The mycelia of Pyricularia oryzae were treated with enzyme solution mixture consisting of Driselase, ${\beta}-Glucuronidase$, Cellulase, and Macerozyme R-10 for the production of protoplasts. More protoplasts were formed from mycelia of race KJ 101 of P. oryzae than that of race KI 315a in the enzyme mixtures. The number of protoplasts was decreased in the untreated control three hrs after the enzyme treatment, whereas the number was increased in the treatments with 10, 50 and 100 mM 2-mercaptoethanol, respectively. The number of protoplasts increased to reach maximum at five hrs after treatment of 10 mM 2-mercaptoethanol, but the least was found in 200 mM. The protoplasts of P. oryzae in a liquid medium containing 2.5% yeast extract, and 2% dextrose reverted to the mycelia after five hrs shaking incubation at $27^{\circ}C$. Some protoplasts produced yeast like buds and the buds were developed to irregularly shaped chains of cell protruded a germ tube like hypha from the distal cell. Once in a while a germ tube like hypha protruded directly from the protoplasts. Except in the first type of reversion, other protoplasts reverted to the normal mycelia. The reversion frequency was highest on PDA with stabilizer of 0.6 M KCl. No reversion of protoplasts occurred on water agar regaardless oftreaatments.

• Food Science and Preservation
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• v.23 no.3
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• pp.402-412
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• 2016

Generation of biogenic amines (BAs) and physiological activities of Makgeolli, Korean rice wine, were investigated during fermentation under different temperatures (20, 25, and $30^{\circ}C$) and time (3, 5, and 7 days). The pH was 3.96~4.36 during fermentation and the acidity increased proportionally with temperature and time. Alcohol and total phenolic contents peaked on day 5 at 25 by 9.0~9.8% and $3.01{\pm}0.07mg/g$, respectively. The organic acid mostly produced was lactic acid, which increased gradually with temperature and time. Formation of biogenic amines comprised of tryptamine and putrescine was observed during fermentation: 6.91, 11.70, and 15.63 mg/mL of tyramine on day 7 at 20, 25, and $30^{\circ}C$, respectively. Antioxidant activities from DPPH and FRAP assay were high in the order of $25^{\circ}C$>$20^{\circ}C$>$30^{\circ}C$ while that from ABTS assay was high regardless of temperature. Processed at 1 mg/mL and $30^{\circ}C$, the inhibitory effect on ${\alpha}$-amylase was $67.99{\pm}0.11$, $73.64{\pm}0.43$, and $75.51{\pm}0.26%$ on days 3, 5, and 7, respectively, which increased proportionally with temperature and time. Inhibitory activity on lipid accumulation in 3T3-L1 adipocytes was the highest in Makgeolli fermented on day 5 at $25^{\circ}C$. This study shows that fermentation at $25^{\circ}C$ for 5 days yields Makgeolli of the best quality with high bioactivities but no biogenic amines.

• Korean Journal of Poultry Science
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• v.18 no.1
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• pp.33-42
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• 1991

In order to study effects of the 9round charcoal and organic acid on the egg production, White Leghorn strain(Hyline) layer of 113 week-old were fed control diet during a week of previous feeding and subsquent experimental diets during 12 weeks of experimental feeding . The experimental diets were the basal (control) diet composed of mainly corn-soybean meal, diet (CPB) substituted 0.5％ of ground charcoal with the defatted rice bran of the control, diet (PWV) added 0.1 mM(based on the acetic acid) of wood vinegar in the CPD and diet(PFA) added 0.1 mM (based on the acetic acid) of fermented acetic acid in the CPD. During experimental feeding period, birds (ed control, CPD and PWV diets showed similar feed intake which was shown a decreasing tendency in birds fed PFA diet as the passage of experimental feeding period. Birds fed experimental diets containing ground charcoal were tended to increase egg weight (g/egg) , while which of birds fed CPD was higher(P＜0.05) than that fed control diet. Chicks fed the control, CPD and PWV diets produced 75.9, 75.0 and 75. 9％ of egg, respectively, which were significantly(P＜0.05) higher than those of birds fed PFA. Daily egg mass(g/bird) were reached to 48.6, 49.9 and 49.5g i,1 layer fed the control, CPD and PWV diets, respectively, showing an increasing tendency in layer fed CPD and PWV compared with those of birds fed control, which were significantly (P＜0.05) higher than those of birds fed PFA . Although the effect of experimental diets on the feed efficiency (egg/feed) was not found, which was shown an increasing tendency in layer fed CPD compared with those in control diet. Compared with the incidence of cracked or shelless eggs in birds fed control diet, which were lowered to 37％ in birds fed CPD diet and 70％ in PWV-fed birds and increased to two times in birds fed PFA . The contents of cholesterol in egg was not affected by experimental diets, while birds fed CPD diet increased significantly(P＜0.05) the daily biosynthesis of egg protein and showed a trend to increase the thickness of eggshell and eggshell contents per eggs compared with that of control diet-fed birds. The results indicated that the ground charcoal powder improved the performance of layer.

• Journal of the Korean association of regional geographers
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• v.6 no.3
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• pp.1-36
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• 2000

Periodic markets of the later Choson dynasty had undergone fundamental changes during the late Chosun dynasty and Japanese colony period. This paper aims to analyse the spatial distributions and changes of the periodic markets in the middle stream of Han-River in this era in the use of the survey of documentary records and fieldwork. Before the early 1910s, long distance transportation was made by riverboats, short distance transportation was done by porters and pack animals. Because goods such as rice, soybean and salt were very heavy and needed long distance transport, they were mainly transported by riverboats. Accordingly, riverports on the shore of river played important roles in exporting and importing goods as nodes of long distance transportation. The opening of railroad Seoul-Busan, the construction of new roads(Sinjakno) of 1910s and the use of oxcarts produced striking changes in the spatial distribution and hierarchy structure of periodic markets. These changes also had influence on the outflow and inflow of goods in the middle stream of Han-River. In the parr of outflow of goods, it seems that the line linking Icheon city-Yongin city-Anseong city played a role in making the boundaries of the marketing areas between goods trams ported by the rail road Seoul-Busan and goods done by Han River river boats. Anseong, Osan and Suwon periodic markets located around railroad stations occupied the higher positions than those of other regions in the hierarchial structure of periodic markets. Their marketing areas could nearly overwhelm those of riverports located in the middle stream region of Han River and extend to the middle stream region of Han River as a result of decrease of transport cost through using of oxcarts and railroads. the opening of railroad Suwon-Yeoju(Suryoson) and railroad Cheonan-Janghawon(Cheonjangson) brought about changes of the structure of long distance trade again. In a part of outflow of goods, it seems that as a result of the new opening of their railroads periodic markets around railroads seized a large portion of the marketing area of the southern part of Yoju and Ichon area and therefore made extreme change in rivershipping of Han River. In the inflow part of goods, goods transported by riverboats from the downstream of Han River before the opening of railroad were imported directly from railroad stations. Accordingly, rivershipping and riverports declined. And because goods were imported by way of great periodic markets and supplied to small periodic markets and consumers before the opening of railroad, but supplied from railroad stations to small periodic markets and consumers after the opening of railroad. The volume of turnover of such great periodic markets as Anseong, Osan and Suwon periodic markets therefore declined. On the other hand, because Yangpyong area had not been yet included within the sphere of influence of railroad until 1930s, it heavily depended on rivershipping of Han River as before. But the opening of railroad Seoul-Wonju(jungangson) brought about decline of rivershipping in Yangpyong and Wonju area.

• Korean Journal of Weed Science
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• v.13 no.2
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• pp.138-150
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• 1993

Experiments were carried out to understand how much do the environmental factors affect growth and tuber formation of arrowhead, Sagittaria trifolia L. The more the light transmittance decreased, the more the numbers of leaves and floral axes decreased. The dry matter weight of tops and the number and the fresh weight of formated tuber were significantly different between the light transmittance of more than 50% and that of less than 30% at the 5% level of DMRT. Plant height, number and width of leaves, and number of floral axis were affected by the Light spectra. And the degree of their effects on growth of arrowhead was different form the light spectrum. The natural light and the clear cellophane film were the most effective to increase the number and the fresh weight of formated tuber and the green spectrum was the least effective to do those. Plant height grown at 0-5cm water depth was shorter than that at 10-20cm water depth. The deeper the water depth was, the lower the leaves number was. The fresh weight and the number of arrowhead tuber were most produced at 0cm water depth and theose were least at 20cm water depth. The shoot growth and the tuber formation of arrowhead was much increased with increase of the application rate of fertilizer. The difference of the transplanted tuber size was not affected at the shoot growth, but tuber formation of arrowhead was increased with increase of the transplanted tuber size. From viewing the effect of temperature after rice heading, the shoot growth and the tuber formation at $35^{\circ}C$ were also higher than those at $25^{\circ}C$.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.3
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• pp.251-259
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• 1987

Humic acids extracted from decomposing plant residues were characterized by infrared(IR) spectra. The IR spectra were further interpreted by chemical analyses for oxygen-containing functional groups such as carboxyl, phenolic, alcoholic, carbonyl, and quinionic groups. 1. The IR spectra obtained in this study were divied into three categories: spectra of humic acids from grain crop straws of rice, barley, wheat and rye produced Type I, while that from wild grass hay yielded Type II, and those from forest tree litter of the deciduous and conifers were led to give Type III. 2. There were no significant changes in the absorption bands observed among humic acids extracted at various stages of decomposition of a given Plant material. 3. The absorption band at about $3,430cm^{-1}$ represents the presence of hydrogen-bonded hydroxyl groups, phenolic-OH groups being the major component. 4. A close relationship was found between the total acidity and the content of phenolic-OH groups of humic acids. The content of carboxyl groups maintains a direct relationship with the content of total hydroxyl groups, and such a close relationship also exists between the content of alcoholic hydroxyls and that of total hydroxyl groups. 5. Overlapping of the absorption bands of carbonyl groups and quinones renders it difficult to make differentiation between the two. 6. A variety of non-armoatic cyclic hydrocarbons appears to be a structural component as evidenced by a sharp absorption peak near $995-1000cm^{-1}$.

• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.196-203
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• 2006

Objective of this research was to determine the effect of application level of fused superphosphate (FSPP) in root media containing granular micronutrient fertilizers (MF) on growth of marigold 'Orange boy' in plug culture. To achieve this, three granular micronutrient mixes such as MF 1, MF 2, and MF 3 were produced and incorporated into three root media, peatmoss+composted pine bark (1:1, v/v, PB), peatmoss+composted rice-hull (1:1, v/v, PR), and peatmoss+composted saw-dust (1:1, v/v, PS), at a rate of $0.3g{\cdot}L^{-1}$. Elevated application level of FSPP in PS medium containing each of MF resulted in increase of plant growth such as plant height, plant width, stem diameter, fresh weight and dry weight at 35days after sowing. The dry weight in the treatment of MF 1, MF 2, MF 3, and control fertilized with $9.0g{\cdot}L^{-1}$ of FSPP were 0.066g, 0.103g, 0.077g, and 0.095g per plant, respectively. These results indicated that each of MF affected marigold plug seedlings differently. The application level of FSPP resulted in the heaviest fresh and dry weight in PR medium were $9.0g{\cdot}L^{-1}$ in MF 1, $3.0g{\cdot}L^{-1}$ in MF 2, $9.0g{\cdot}L^{-1}$ in MF 3, and $6.0g{\cdot}L^{-1}$ in control. Elevated application level of FSPP in PB medium containing each of the micronutrient mix increased fresh and dry weight. The general trends in response of plant growth to elevated FSPP were linear and quadratic. The dry weight in the treatment of MF 1, MF 2, MF 3, and control fertilized with $9.0g{\cdot}L^{-1}$ of FSPP in PB medium were 0.131g, 0.104g, 0.137g, and 0.111g per plant at 35days after sowing. These results indicated that MF 1 and MF 3 performed better than MF 2 and control treatments in growth of marigold plug seedlings.

• Applied Microscopy
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• v.40 no.2
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• pp.73-80
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• 2010

Liver regeneration is a result of highly coordinated proliferation of hepatocytes and nonparenchymal liver cells. Partial hepatectomy (PH) is the most often used stimulus to study liver regeneration because, compared with other methods that use hepatic toxins, it is not associated with the tissue injury and inflammation, and the initiation of the regenerative stimulus is precisely defined. Granulocyte macrophage-colony stimulating factor (GM-CSF), which is a cytokine able to regulate the proliferation and differentiation of epithelial cells, was first identified as the most potent mitogen for bone marrow. Particularly, rrhGM-CSF, which is highly glycosylated and sustained longer than any other types of GM-CSF in the blood circulation, was specifically produced from rice cell culture. In this experiment, effects of rrhGM-CSF administration were evaluated in the regenerating liver after 78% PH of rats. Morphological changes induced by PH were characterized by destroyed hepatocyte plate around the central vein and enlarged nuclear cytoplasmic ratio and increased hepatocytes with two nuclei. And then, proliferation of liver cells (parenchymal and nonparenchymal) and rearrangement of plates and lobules seemed to be carried out during liver regeneration. These alterations in the experimental group preceded those of the control. Since proliferating cell nuclear antigen (PCNA) is known to be a nuclear protein maximally elevated in the S phase of proliferating cells, the protein was used as a marker of liver regeneration after PH in rats. PCNA levels by western blot analysis and immunohistology were compared between the two groups. PCNA protein expression of two groups at 12 hr and 24 hr after injury showed similar pattern. The protein expression showed the peak at 3 days in both groups, however, the protein level of the experimental group was higher than that of the control. On immunohistochemical observations, the reaction product of PCNA was localized at the nuclei of proliferating cells and the positive reaction in experimental group at 3 days was clearly stronger than that in control group. The results by Western blotting and immunohistology for PCNA showed similar pattern in terms of the protein levels. In conclusion, rrhGM-CSF administration during liver regeneration after 78% PH accelerated breakdown and restoration of the hepatic plate and expression of PCNA. These results suggest that rrhGM-CSF might play an important role during liver regeneration in rats.