• Title/Summary/Keyword: restriction

Search Result 4,051, Processing Time 0.03 seconds

Tests of Land Boundary Using Orthoimages (정사영상을 이용한 토지경계설정 실험)

  • Lee, Young-Jin;Ryu, Su-Hyun;Kim, Ho-Young;Park, Jung-Hwan
    • Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
    • /
    • 2010.04a
    • /
    • pp.289-290
    • /
    • 2010
  • Orthoimage, digital map, cadastral map, and areas and district were converted and overlaid so as to apply legal restrictions on boundaries set by a land object concept restriction line and to analyze boundaries formed by overlaying areas and districts and characteristics of restrictions. With the restriction lines made on the basis of orthoimage.

  • PDF

Isolation of kpn I restriction endonuclease from klebsiella pneumonia (Klebsiella pneumonia로 부터 제한효소 Kpn I의 분리)

  • 이상철;이대실;유명희
    • Korean Journal of Microbiology
    • /
    • v.25 no.1
    • /
    • pp.73-79
    • /
    • 1987
  • A restriction endonuclease, Kpn I has been isolated from Klebsiella pneumonia. Cells were broken by sonication. After ultracentrifugation the supernatant containing Kpn I activity was further purified by Sepharose-6B gel filtration, DEAD-Cellulose, Heparin-Agarose, and Aminohexyl-Agarose column chromatography. Final enzyme preparation was essentially free of contamination exonuclease and phosphatase, as judged by ligation-recut test. Total activity of the enzyme recovered from 10 grams of cells was $4.7\times 10^5$ units.

  • PDF

Rapid plasmid mapping computer program (Plasmid의 제한효소 지도 작성을 위한 콤퓨터 프로그램)

  • 이동훈;김영준;이승택;강현삼
    • Korean Journal of Microbiology
    • /
    • v.24 no.1
    • /
    • pp.12-17
    • /
    • 1986
  • A new computer algorithm is described to order the restriction fragments of plasmid DNA which has been cleaved with several restriction endonucleases in single or double digestions rapidly with realistic error rates. The permutation and high weight on small fragments methods construct all logical circular map solutions. The program is written in Apple BASIC and run on an Apple II plus microcomputer with 64K memory. Several examples are presented which indicate the high efficiency of the profram in construction possible restriction map for YEp24.

  • PDF

RESTRICTION OF SCALARS WITH SIMPLE ENDOMORPHISM ALGEBRA

  • Yu, Hoseog
    • Korean Journal of Mathematics
    • /
    • v.30 no.3
    • /
    • pp.555-560
    • /
    • 2022
  • Suppose L/K be a finite abelian extension of number fields of odd degree and suppose an abelian variety A defined over L is a K-variety. If the endomorphism algebra of A/L is a field F, the followings are equivalent : (1) The enodomorphiam algebra of the restriction of scalars from L to K is simple. (2) There is no proper subfield of L containing LGF on which A has a K-variety descent.

A Study on the Train Speed Restriction to Prevent Track Buckling in the Hot Summer (혹서기 좌굴사고 방지를 위한 합리적인 열차속도 규제에 관한 연구)

  • Bae, Hyun-Ung;Choi, Jin-Yu;Kim, Myoung-Su;Lee, Chin-Ok;Lim, Nam-Hyoung
    • Proceedings of the KSR Conference
    • /
    • 2011.10a
    • /
    • pp.2891-2894
    • /
    • 2011
  • Recently, the rise of the rail temperature is accelerating further due to the global warming, and the track stability(buckling) problem result from the axial force in the continuous welded rail(CWR) have become the most important issue. This track stability threatens the security of running trains in the hot summer. In order to prevent the track buckling in the hot summer and ensure the safety of the running train, as a part of the safety control plan for KORAIL high-speed railway, the train speed restriction according to the rail temperature was introduced in 2004. However, the conceptual and theoretical background of train-speed restriction is uncertain. In this paper, the theoretical study about the reasonable train-speed restriction is performed. For this purpose, the risk-based probabilistic stability evaluation of the track buckling is applied.

  • PDF

Effect of Type of Body Shape Perception on Health Concern, Depression, Dietary Restriction, and Exercise Practice among University Students (대학생의 체형지각 유형이 건강관심, 우울, 식이제한 및 운동실천에 미치는 영향)

  • Ham, Young Lim;Park, Mijeong
    • Journal of muscle and joint health
    • /
    • v.20 no.2
    • /
    • pp.151-160
    • /
    • 2013
  • Purpose: The purpose of this study was to identify the effect of type of body shape perception on health concern, depression, dietary restriction and exercise practice in university students. Methods: A descriptive design was used. Data were collected from 308 university students by using structured questionnaires. Results: The results showed that 65.6% of the students believe that they are overweight. There are no differences in health concern, depression, and exercise practice among the skinny perception group, satisfaction group, and obesity perception group. However, there was a significant difference in dietary restriction among the three groups (p<.001). The type of body shape perception was significantly associated with dietary restriction (p=.007). Conclusion: Integrated and effective interventions are necessary for university students to establish healthy body image and learn healthy weight control.

Study on PDMS/Class Microthermostat Fabrication and Evaluation for Restriction Enzyme Reaction (제한효소 반응용 PDMS/유리 마이크로 항온조 제작 및 특성평가)

  • Jin Seok-Ho;Cho Yong-Jin;Ahn Yoomin
    • Transactions of the Korean Society of Mechanical Engineers A
    • /
    • v.28 no.10
    • /
    • pp.1598-1602
    • /
    • 2004
  • In this paper, we report a microthermostat using PDMS (poly-dimethylsiloxane) and glass. This PDMS/glass chip is able to maintain constant temperature that is necessary for restriction enzyme reaction. Since PDMS is the low-cost and the mass-producible material and has very good biochemical compatibility, PDMS chip has more benefit than general Si chip. Heater was made of Au wiring patterned on Pyrex glass. A reaction chamber has a capacity of about 3 ${mu}ell$. We performed a restriction enzyme reaction by using the fabricated microthermostat and conventional method. Then, with the electrophoresis, we made a comparison between the result from the micro reactor and the one from conventional method.

PDMS/Glass Based DNA Microbiochip for Restriction Enzyme Reaction and Electrophoresis Detection (DNA의 제한효소 반응 및 전기영동 검출용 PDMS/유리 마이크로바이오칩)

  • Choi Joon-Young;Ahn Yoo Min;Hwang Seung-Yong
    • Transactions of the Korean Society of Mechanical Engineers A
    • /
    • v.30 no.1 s.244
    • /
    • pp.26-31
    • /
    • 2006
  • This paper reports low-cost PDMS/glass based DNA microbiochip for the restriction enzyme reaction and its products detection using the capillary electrophoresis. The microbiochip ($25mm{\times}75mm$) has the heater integrated reactor ($5{\mu}{\ell}$) for DNA restriction enzyme reaction at $37^{\circ}C$ and the microchannel ($80\;{\mu}m{\times}100\;{\mu}m{\times}58mm$) for the capillary electrophoresis detection. It is experimentally confirmed that the digestion of the plasmid ($pGEM^{(R)}-4Z$) by the enzyme (Hind III and Sca I) is performed for less than 10 min and its electrophoresis detection is able to sequentially on the fabricated microbiochip.

Restriction and transcription maps of mitochondrial DNA of trimorphomyces papilionaceus

  • Jeoung, Won-Jin;Hong, Soon-Gyu;Won, Kang-Young;Jung, Hack-Sung
    • Journal of Microbiology
    • /
    • v.33 no.2
    • /
    • pp.149-153
    • /
    • 1995
  • Mitochondrial DNA has been isolated from Trimorphomyces papilionaceus. By analyzing DNA fragments digested by restriction enzymes, a restriction site map has been constructured. The mtDNA of T. papilionaceus amounts to 48.5 kb in size and is circular in structure. Entire mitochondrial DNA was cloned in E coli plasmids and Northern blot hybridization was done using cloned and subcloned DNAs as probes. Based on hybridization results of mitochondrial RNA transcripts, a transcription map was prepared.

  • PDF

Effects of an Anabolic Steroid, Nandrolone Phenylpropionate, on Reductions in Body and Muscle Proteins Under the Dietary Regimens of Feeding a Low-Protein Diet and of 50% Food Restriction in Rats

  • Choo, Jong-Jae
    • Nutritional Sciences
    • /
    • v.1 no.1
    • /
    • pp.3-7
    • /
    • 1998
  • The aim of the present investigation was to see whether an anabolic steroid, nandrolone phenylpropionate (NPP), exerts protienanabolic effects under such adverse nutritional conditions as protein deficiency and protein-energy malnutrition in male rats. feeding on a low-protein (8% casein) diet resulted in a marked reduction in body weight gain that was associated with reductions in body protein and protein content of gastrocnemius muscle. Administration of NPP (4 mg/kg body weight) did not alter muscle and body protein depletion induced by a low-protein diet. 50% food restriction caused reductions in body protein and in protein content of gastrocnemius muscle. These reductions were partially prevented by NPP (4 mg/kg body weight). Food restriction did not affect plasma concentration of corticosterone, insulin, or tetosterone plus dihydrotestosterone. On the other hand, neither plasma concentration of corticosterone nor insulin were affected by NPP. The present results show that anabolic steroids do not express anabolic effects under conditions of protein deficiency, but in protein-energy malnutrition, anabolic steroids exert their anabolic effects even in male rats.

  • PDF