Korean Journal of Microbiology (미생물학회지)
- Volume 25 Issue 1
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- Pages.73-79
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- 1987
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Isolation of kpn I restriction endonuclease from klebsiella pneumonia
Klebsiella pneumonia로 부터 제한효소 Kpn I의 분리
Abstract
A restriction endonuclease, Kpn I has been isolated from Klebsiella pneumonia. Cells were broken by sonication. After ultracentrifugation the supernatant containing Kpn I activity was further purified by Sepharose-6B gel filtration, DEAD-Cellulose, Heparin-Agarose, and Aminohexyl-Agarose column chromatography. Final enzyme preparation was essentially free of contamination exonuclease and phosphatase, as judged by ligation-recut test. Total activity of the enzyme recovered from 10 grams of cells was