• Restorative Dentistry and Endodontics
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• v.19 no.2
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• pp.499-512
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• 1994

The purpose of this study was to evaluate the microleakage electrochemically using different retrograde filling materials, different root resection angle, and different cavity preparation instruments. 104 extracted single-rooted teeth were selected for this study. 100 teeth were used as experimental groups and four was used as controls. Anatomical crowns were resected, root canals were prepared, and the apical 2 mm of roots were removed. The experimental roots were randomly divided into five equal groups. Experimental groups : Group 1. no bevel, cavity preparation with ultrasonic instruments, amalgam filling Group 2. no bevel, cavity preparation with ultrasonic instruments, SuperEBA cement filling Group 3. no bevel, cavity preparation with ultrasonic instruments, desiccated ZOE filling Group 4. $45^{\circ}$ bevel, cavity preparation with ultrasonic instruments, amalgam filling Group 5. no bevel, cavity preparation with conventional bur, amalgam filling Microleakage was measured once a day for 30 days using electrochemical method and were analyzed statistically. The results were as follows : 1. The group with Super EBA cement filling showed the least marginal leakage from second to fourth day(p<0.05), there was no significant difference between the group with amalgam filling during eighth to eighteenth day(p>0.05), but after the nineteenth day here was a higher marginal leakage than the group with amalgam filling(p<0.05). 2. The group with desiccated ZOE filling demostrated that the highest marginal leakage, started on the eighth day(p<0.05). 3. The group using ultrasonic instrument showed lower marginal leakage than the group using bur until the nineteenth day(p<0.05), but there was no significant differnce with the group using bur after twentythird day(p>0.05). 4. The group without bevel showed lower marginal leakage than the group with bevel (p<0.05). 5. Whether bevel or nor had much more effect on marginal leakage than with cavity perparation instrument when the cavity was retrogrdefilled with amalgam(stepwise regression).

• Restorative Dentistry and Endodontics
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• v.19 no.2
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• pp.409-428
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• 1994

Endodontic surgery is performed when conventional endodontic therapy fails or is contraindicated. In such cases, retrograde filling materials including amalgam, composite resin, and various cements have been used. Biocompatibilty and margin sealing ability of retrograde filling materials are important for the long term success of endodontic surgery. In vitro cell culture is frequently used as the method of measuring the biocompatibilty of dental materials. The purpose of this study was to evaluate the cytotoxicity of six kinds of retrograde filling materials including newly developed light curing glass ionomer cements. Each material was mixed according to. the manufacture's instruction and evaluated as : freshly mixed, 24-hour after mixing, and 168-hour after mixing respectively. The elution solution was extracted after 24-hour contact with materials using media. Cytotoxicity was evaluated by direct contact, or elution contact. Test results of radiochromium($^{51}Cr$) release, cell viability using tetrazolium dye (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl dimethyltetrazolium bromide(MTT) test and lactate dehydrogenase(LD) of damaged L929 cells were analyzed. In the $^{51}Cr$ release of direct contact, all experimental retrograde filling materials except amalgam and glass ionomer cement showed increased cytotoxicity compared to control. In the $^{51}Cr$ release of elution solution, the released $^{51}Cr$ was so minimal that it was impossible. to evlauate the cytotoxicity exactly. The elution solutions of glass ionomer cement and IRM showed marked cytotoxicity in MTT test. LD enzyme activity was highest in tests of direct contact with composite, light curing composite, and light curing glass ionomer cement and IRM. Amalgam revealed least cytotoxicity while IRM showed cytotoxicity using all three methods. Composite, light curing composite and light curing glass iomomer cement were cytotoxic in the tests of $^{51}Cr$ release and LD activity. Glass ionomer cement showed cytotoxic effect only in the MTT method. From these results it is suggested that the standardization and optimization of cytotoxicity testing, especially using elution solutions, should be strongly advised.

• Restorative Dentistry and Endodontics
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• v.19 no.2
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• pp.384-408
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• 1994

The purpose of this study was to evaluate the fracture resistance of tooth restored with gold inlay. A profound understanding of the isthmus width factor, which is one of the several parameters of cavity designs, would facilitate the appropriate cavity preparation in a specific clinical situation. In this study, the cavities for gold inlay were prepared in maxillary left first premolar. A three-dimensional model was designed using I-DEAS program. The model was composed of 2515- nodes and 2172 isoparametric brick elements. In the model isthmus width was varied into 1/4, 1/3 and 1/2 of intercuspal width respectively, and numeric values of the material properties of enamel, dentin and gold was set. Three types of load : concentrated load, divided load and distributed load was 500N. The empty cavities in the model were also examined using divided load and distributed load. The three - dimensional Finite Element Method was used to analysis the displacement and stress distribution. The results were as follows : 1. All of the experimental models which were filled with gold inlay revealed similar direction of displacement to that of the natural tooth model under the same load type. But in the models with empty cavities, as the isthmus width increased, the degree of displacement increased in the case of divided load type. 2. All experimental models which were filled with gold inlay showed stress concentration at load points, but in the models with empty cavities at divided load type, as isthmus width increased, stress was concentrated at the comer of the pulpal floor. 3. In the models with empty cavities at divided load type, tooth fracture was expected regardless of isthmus width, but all experimental models which were filled with gold inlay after cavity preparation were not susceptible to fracture. 4. In all experimental models which were filled with gold inlay after cavity preparation, displacement patterns were similar under both concentrated and divided load types. In the models with empty cavities, a divided load resulted in a bucco-lingual cuspal displacemenat in both sides, but a distributed load resulted in a lingual displacement of the tooth.

• Restorative Dentistry and Endodontics
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• v.14 no.1
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• pp.179-188
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• 1989

The purpose of this study was to measure Micro vicker's hardness of 4 kinds of anterior Composite resins (Pyrofil light bond anterior, Lite-fil anterior, Photo clear fil anterior, Silux) and 6 kinds of posterior Composite resin (Pyrofil light bond posterior. Lite-fil posterior, Photo clear fil posterior, Occlusin posterior, Palfique light posterior, P-30, posterior) according to deference of depth and distance of light tip from surface of composite resin. Each composite resin was filled into Teflon tube of 5mm in diameter and 5mm in depth, celluloid matrix was covered and the light in accordance with each composite resin was irradiated in distance of zero millimeter and 1 cm from light tip to surface of composite resin for 30 seconds. Specimens were sectioned longitudinally with cutting device. Microvicker's hardness measurements ware made at the depth of surface, 1mm, 2mm, 3mm, 4mm and 5mm from the surface to deep portion. Vicker's hardness numbers were taken on each depth under 200gm load for 30 seconds with MVK-E. The following results were: 1. The highest hardness value was measured at 1 mm depth. Then the deeper the depth, the lesser the hardness was observed. 2. The hardness value of anterior composite resins is lower than one of posterior composite resins. 3. Hardness number of composite resin irradiated in distance of zero millimeter from surface of composite resin was higher than one of 1 cm from surface of composite resin. 4. The pattern of hardness change at varying depth was similar to all the experimental material with no relation to distance of light from specimen.

• Restorative Dentistry and Endodontics
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• v.14 no.1
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• pp.173-177
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• 1989

As widely known major cause of tooth discoloration is red blood cell in dentinal tubules after tooth trauma and extirpation. Hemoglobin in the red cells easily conbine with hydrogen sulfide and change natural tooth color into dark brown. To confirm and compare the effect of bleaching agents test material was made as fellows: No. 40 paper points were soaked in blood and put into the oven for 24 hours to dry. Dried paper points were again soaked in diluted yolk by distilled water for a minute and kept the points for a week to get fully sulfide. Hydrogen peroxide (25%), Sodium hypochlorite (2%), Sodium hyposulfate (2%), Sodium perborate and mixture of Hydrogen peroxide (25%) and Sodium perborate were employed as bleaching agents. About 0.2ml of each medicaments were put into the small test tubes and blood soaked paper points were gently placed vertically with the apex downward and kept 24 hours in the oven ($37^{\circ}C$). Every 24 hours the medicaments were renewed at 24 and 48 hours. Following are the results of the study: 1. Significant bleaching effect was found on the case of hydrogen peroxide (25%) after 24 hours. The area above 2mm from apex also fully bleached after 48 hours. 2. A mixture of hydrogen peroxide and sodium perborate treated case showed moderate effect at 24 hours and significant effect at 48 hours. High portion of the point (above 2mm) was bleached satisfactory at 72 hours. 3. The effect of sodium perborate at 48 hours showed moderate and significant at 72 hours. 4. Sodium hypochlorite and sodium hyposulfate were weak agent in bleaching. At 72 hours case revealed moderate effect and influenced on high portion of the point very weak effect.

• Restorative Dentistry and Endodontics
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• v.15 no.2
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• pp.1-16
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• 1990

This study was to evaluate the cytotoxic effect in vitro and the tissue response within the rat peritoneal cavity to high copper amalgam and glass ionomer-silver cement, suggested for use as a retrograde endodontic filling material. In the cytotoxicity experiment, the radioactively ($^{51}Cr$) labeled L929 mouse fibroblasts were employed to determine the relative cytotoxicity of two experimental materials. Those materials were evaluated immediately after set and after one and seven days setting. In the tissue response experiment, two experimental materials were to evaluate mean peritoneal cellular count, differential cell count and the content of silver and copper in pooled packed cells and eluate samples taken by peritoneal lavage technique, and compared with surgical control after one day. two, four and six weeks of implantation. The results were as following: 1. High copper amalgam exhibited significant cytotoxicity immediately after set but showed no sign of toxicity after one day and seven days setting materials. 2. Glass ionomer-silver cement showed no sign of toxicity immediately after set and after one day and seven days setting. 3. High copper amalgam and glass ionomer-silver cement groups produce no significant difference in the mean peritoneal cell count when compared with the surgical control group after one day, two and four weeks of implantation. Surgical control group exhibited significantly a greater cell count when compared with the High copper amalgam group after six weeks. 4. High copper amalgam group increased significantly in the percentage macrophages after four and six weeks of implantation when compared with surgical control group. 5. The trace metal analysis involved an increased silver content in the elutes and an increased copper content in the packed cells of high copper amalgam group, and an increased silver content in the packed cells and elutes of glass ionomer-silver cement group.

• Restorative Dentistry and Endodontics
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• v.23 no.2
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• pp.586-596
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• 1998

Amalgam is one of the most commonly used dental restoration material because of its convenience, economic and physical properties. But microleakage in the tooth and amalgam interface has been its major problem, and many efforts have been made to overcome this shortcoming. The purpose of this study is to compare the effect of various liners on microleakage of amalgam restoration. Cavities were prepared on the buccal or lingual surface of ninety sound, extracted human premolars and six different liners (Tubulitec$^{(R)}$, Superbond D-liner II Plus$^{(R)}$, Superbond D-liner II Plus$^{(R)}$ with polymer, Scotchbond Multipurpose Plus$^{(R)}$, Copalite$^{(R)}$, No liner) were unapplied according to manufacturer's instructions and amalgam had been condensed immediately. The specimens were thermocycled by dipping in methylene blue dye at $5^{\circ}C$ and $55^{\circ}C$ for 1500 cycles. The amalgam-tooth interface was examined under stereobinocular microscope and the dye penetration was scored. The results were as follows : 1. The Tubulitec$^{(R)}$ group showed less microleakage than no liner or Copalite$^{(R)}$ group (p<0.01). 2. The Tubulitec$^{(R)}$, Superbond D-liner II Plus$^{(R)}$, Superbond D-liner II Plus$^{(R)}$ with polymer and Scotch bond Multipurpose Plus$^{(R)}$ groups were not significantly different. 3. The Copalite$^{(R)}$ and Scotchbond Multipurpose Plus$^{(R)}$ groups were not significantly different. Using the polystyrene liner and resin liners under admixed type of high-copper amalgam restoration significantly reduced microleakage in the tooth-amalgam interface. Further clinical studies on polystyrene liner are recommended.

• Restorative Dentistry and Endodontics
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• v.23 no.2
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• pp.682-689
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• 1998

The purpose of this study was to evaluate the sealing ability of the Microseal$^{(R)}$, which was new obturation system made by Tycom company, U.S.A. Forty-five extracted single-rooted human teeth were resected at cemento-enamel junction and divided three groups. All canals were prepared using Profile system, and then each group was obturated by lateral condensation technique (group 1), vertical condensation technique (group 2) and Microseal$^{(R)}$ condensation technique (group 3) with root canal sealer. Teeth were immersed in resorcinol-formaldehyde resin for 5 days at $4^{\circ}C$ and the resin was allowed to polymerize completely for 4 days at room temperature. Teeth were resected horizontally at 1 mm (level I), 2 mm (level II), 3 mm (level III) from the anatomical root apex using low speed microtome and examined with Image analyzer (IBASR, Zeiss co., Germany.) at ${\times}25$ magnification. The gab between the canal wall and the filling material, which was filled with the resin, was measured at each of the three levels. Each ratio of leakage was expressed percentage by calculating the ratio of the area of the resin to the total area of the canal and was analyzed statistically (one-way ANOVA). The results were as follows; 1. The mean ratio of leakage (%) was 6.46% at group 1, 3.06% at group 2, 11.27% at group 3. 2. When evaluating the ratio of leakage at the three levels, there was level I> level II> level III in all groups. Especially, the difference between level I and level III was statistically significant (p<0.05). 3. When evaluating the ratio of leakage at the three groups, there was group 3> group 1> group 2 at all levels. Especially the difference between group 2 and group 3 was statistically significant (p<0.05).

• Restorative Dentistry and Endodontics
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• v.22 no.1
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• pp.93-109
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• 1997

Finding a right repair material for furcation perforation is one of the major issues in clinical endodontics. In this experiment, three materials, calcium sulfate, amalgam, and calcium hydroxide were tested for perforated furcation repair. Sixty premolars and molars of five dogs were used. A #4 round bur was used to create the perforation. All experimental teeth were divided into two repair-time groups. One was immediate-repair group, where the perforation was repaired immediately, the other was delayed-repair group, where the perforation was left open for four weeks and then repaired with the same manner as in the immediate-repiar group. All chamber openings were sealed with amalgam and then radiographed. The animals were sacrificed at eighth week following the repair procedure. Radiographic evaluation for furcal bone destruction was done. Histologic evaluation was ranked as 0,1,2,3 according to the inflammation degrees. New bone formation was also recorded. The following conclusions were drawn within the limits of the experimental results: 1. In immediate-repair group, no significant differences existed between the materials. 2. In delayed-repair group, calcium sulfate showed significantly less furcal bone destruction and lower inflammation degree than amalgam.(p<0.05) 3. Overextruded specimens showed more severe inflammation than unextruded specimens. 4. Most of the specimens showed certain degrees of inflammatory reaction and incomplete hard tissue healing. 5. In delayed-repair group, treated group showed less inflammation than untreated control group.

• Restorative Dentistry and Endodontics
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• v.39 no.3
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• pp.180-186
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• 2014

Objectives: The purpose of this in vitro study was to evaluate the microleakage and penetration of fissure sealant in permanent molar teeth with fluorosis after pretreatment of the occlusal surface. Materials and Methods: A total of 120 third molars with mild dental fluorosis were randomly divided into 6 groups (n = 20). The tooth surfaces were sealed with an unfilled resin fissure sealant (FS) material. The experimental groups included: 1) phosphoric acid etching (AE) + FS (control); 2) AE + One-Step Plus (OS, Bisco) + FS; 3) bur + AE + FS; 4) bur + AE + OS + FS; 5) Er:YAG laser + AE + FS; and 6) Er:YAG laser + AE + OS + FS. After thermocycling, the teeth were immersed in 0.5% fuchsin and sectioned. Proportions of mircoleakage (PM) and unfilled area (PUA) were measured by digital microscope. Results: Overall, there were significant differences among all groups in the PM (p = 0.00). Group 3 showed the greatest PM, and was significantly different from groups 2 to 6 (p < 0.05). Group 6 showed the lowest PM. Pretreatment with Er:YAG with or without adhesive led to less PM than bur pretreatment. There were no significant differences among groups in PUA. Conclusions: Conventional acid etching provided a similar degree of occlusal seal in teeth with fluorosis compared to those pretreated with a bur or Er:YAG laser. Pretreatment of pits and fissures with Er:YAG in teeth with fluorosis may be an alternative method before fissure sealant application.