Purpose : Toll-like receptor 2 (TLR2) is critical in the immune response to mycobacterial infections. The purpose of this study was to analyze TLR2 surface expressions and TLR2-mediated tumor necrosis factor-alpha ($TNF-{\alpha}$) and interleukin-6 (IL-6) production in patients with BCG vaccine-associated suppurative lymphadenitis. Methods : Peripheral monocytes were separated from 16 patients with BCG vaccine-associated suppurative lymphadenitis and 10 healthy controls using a magnet cell isolation kit. Monocytes ($1{\times}10^6$ cells/well) were incubated with a constant amount of $Pam_3CSK_4$ ($100{\mu}g/mL$) for 24 hours. TLR2 surface expression on monocytes was analyzed by FACS analysis and TLR-2 mRNA expression was determined by RT-PCR. TLR2-mediated $TNF-{\alpha}$ and IL-6 production were measured by ELISA. Results : In patients with BCG vaccine-associated suppurative lymphadenitis, low TLR2 expression on monocytes ($3.39{\pm}$1.2% versus $4.64{\pm}2.6%$) together with significantly lower TLR2 mRNA expression than in the healthy controls was seen after $Pam_3CSK_4$ stimulation. TLR2-mediated $TNF-{\alpha}$ and IL-6 production in patients with BCG vaccine-associated suppurative lymphadenitis ($TNF-{\alpha}$, $775.5{\pm}60.8pg/mL$; IL-6, $4,645.8{\pm}583.9pg/mL$) were also lesser than that in healthy controls ($TNF-{\alpha}$, $1,098.5{\pm}94.3pg/mL$; IL-6, $6,696.3{\pm}544.3pg/mL$). Conclusion : These findings suggest that low TLR2 expression on monocytes might be associated with increased susceptibility to BCG vaccine-associated suppurative lymphadenitis.
This study was conducted to investigate the response of growth and yield of rice under five different cultural methods, machine transplanting(MTNT), puddled drill seeding (PDSNT), drill seeding on soil surface (DSNT) , broadcasting on soil surface (BSNT) under no-tillage paddy condition and conventional machine transplanting(MTT) in Jeonbuk series(siltyloam soil) from 1993 to 1995. Soil hardness was higher in no-tillage soil and increased with highly difference between tillaged and no-tillage soil with deeper soil depth. Bulk density was heavier in no-tillage soil and porosity was higher in tilled soil than that of the control. The rate of effective tiller was higher in MTT, following MTNT, PDSNT, DSNT and BSNT. Weed occurrence was more serious in no-tillage soil, than that of tillaged soil. The rate of lower internode length was lower in DSNT and BSNT and was similar with MTT in PDSNT and MTNT. Height of center gravity in terms of lodging tolerance was lower in direct seeding than in machine transplanting. Depth of buried culm was shorter in no-tillage soil, especially in DSNT and BSNT. Total amount of root was higher in MTT, following MTNT, PDSNT, BSNT and BSNT and the distribution rate of root in shallower soil layer was higher in no-tillage soil, especially in BSNT and DSNT. Field lodging occured highly in BSNT, following DSNA, PDSNT and MTNT with high lodging scale in DSNT and BSNT. Panicle number per unit land square meter was the highest in MTT and the least in BSNT. Ripened grain ratio was low in BSNT and DSNT due to heavy lodging. Yield of milled rice was 93% in PDSNT, 87% in DSNT, 81% in BSNT and 96% in MTNT, compared with 534kg /10a in MTT.
Ji, Gwang-Su;Yu, Dae-Heon;Lee, Seong-Gu;Kim, Jae-Hyu;Ji, Yeong-Hun
The Journal of Korean Society for Radiation Therapy
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v.8
no.1
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pp.19-27
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1996
I. Project Title A Study of Brachytherapy for intraocular tumor II. Objective and Importance of the project The eye enucleation or external-beam radiation therapy that has been commonly used for the treatment of intraocular tumor have demerits of visual loss and in deficiency of effective tumor dose. Recently, brachytherapy using the plaques containing radioisotope-now treatment method that decrease the demerits of the above mentioned treatment methods and increase the treatment effect-is introduced and performed in the countries, Our purpose of this research is to design suitable shape of plaque for the ophthalmic brachytherapy, and to measure absorbed doses of Ir-192 ophthalmic plaque and thereby calculate the exact radiation dose of tumor and it's adjacent normal tissue. III. Scope and Contents of the project In order to brachytherapy for intraocular tumor, 1. to determine the eye model and selected suitable radioisotope 2. to design the suitable shape of plaque 3. to measure transmission factor and dose distribution for custom made plaques 4. to compare with the these data and results of computer dose calculation models IV. Results and Proposal for Applications The result were as followed. 1. Eye model was determined as a 25mm diameter sphere, Ir-192 was considered the most appropriate as radioisotope for brachytherapy, because of the size, half, energy and availability. 2. Considering the biological response with human tissue and protection of exposed dose, we made the plaques with gold, of which size were 15mm, 17mm and 20mm in diameter, and 1.5mm in thickness. 3. Transmission factor of plaques are all 0.71 with TLD and film dosimetry at the surface of plaques and 0.45, 0.49 at 1.5mm distance of surface, respectively. 4. As compared the measured data for the plaque with Ir-192 seeds to results of computer dose calculation model by Gary Luxton et al. and CAP-PLAN (Radiation Treatment Planning System), absorbed doses are within ${\pm}10\%$ and distance deviations are within 0.4mm Maximum error is $-11.3\%$ and 0.8mm, respectively. As a result of it, we can treat the intraocular tumor more effectively by using custom made gold plaque and Ir-192 seeds.
Fruit bodies similar to the Phellinus sp. residing on the mulberry were collected at Yang-yang in Kang-won-do province and one strain of Phellinus sp. was isolated from the fruit bodies. For mass production of the isolated mycelia in a submerged culture, the culture conditions, medium composition, and the effect of various culture systems on the mycelial growth, were investigated. The morphological characteristics of the fruit body were as follows: covered with blackish to black and rough, lower surface with yellowish-brown to dull-brown and smooth, 5-7 cm thick and hard woody. Also, the pure cultured mycelia showed yellowish-brown color, capability of purplish-brown pigment production on the PDA plate media, no-formation of clamp-connection, much binding branch, and enzyme activities such as laccase, tyrosinase and peroxidase. Therefore, pure cultured strain was identified to be Phellinus sp. In the flask culture, the optimum culture conditions for the mycelial production were obtained after cultivation of 8 days at inoculum level of 5%(v/v), media volume of 70 mL, 150 rpm, initial pH 6, and temperature of $30^{\circ}C$. Optimum medium composition from the response surface analysis were determined to be glucose 12.12 g/L, sucrose 12.12 g/L, yeast extract 11.15 g/L, malt extract 11.15 g/L, $KH_2PO_4$ 0.855 g/L and $CaCl_2$ 0.855 g/L. The production of the mycelia after 4 and 8 days of cultivation was 1.95 and 9.89 g/L, respectively. The maximum specific growth rate and productivity were $0.020\;hr^{-1}$ and 1.25 g/L/day, respectively. Among the three different culture systems for the growth of mycelia, the maximum mycelial dry weight of 7.5 g/L was obtained after cultivation of 4 days in the air-lift fermentor under aeration rate of 2.5 vvm. The maximum specific growth rate and productivity were $0.033\;hr^{-1}$ and 1.9 g/L/day, respectively, which were about 1.7 and 4.2 times higher than those of flask culture.
Modeling of longterm runoff is theoritically based on waterbalance analysis. Simplified equation of water balance with rainfall, evapotranspiration and soil moisture storage could be formulated into regression model with variables of rainfall, pan evaporation and previous-month streamflow. The hydrologic response of water shed could be represented lumpedly, qualitatively and deductively by regression coefficients of water-balance regression model. Characteristics of regression modeling of water-balance were summarized as follows; 1. Regression coefficient $b_1$ represents the rate of direct runoff component of precipitation. The bigger the drainage area, the less $b_1$ value. This means that there are more losses of interception, surface detension and transmission in the downstream watershed. 2. Regression coefficient $b_2$ represents the rate of baseflow due to changes of soil moisture storage. The bigger the drainage area and the milder the watershed slope, the bigger b, value. This means that there are more storage capacity of watershed in mild downstream watershed. 3. Regression coefficient $b_3$ represents the rate of watershed evaporation. This depends on the s oil type, soil coverage and soil moisture status. The bigger the drainage area, the bigger $b_3$ value. This means that there are more watershed evaporation loss since more storage of surface and subsurface water would be in down stream watershed. 4. It was possible to explain the seasonal variation of streamflow reasonably through regress ion coefficients. 5. Percentages of beta coefficients what is a relative measure of the importance of rainfall, evaporation and soil moisture storage to month streamflow are approximately 89%, 9% and 11% respectively.
The Baegdusan volcano is one of the most active volcanoes in northeastern Asia, and the 10th century eruption was the most voluminous eruption in the world in recent 2,000 years. During the period from 2002 to 2005, volcanic earthquakes and abnormal surface distortions by suspected subsurface magma intrusion beneath the volcano were observed in the Baegdusan area. Seismic activity has gradually increased with earthquake swarms during 2002-2003 and hundreds of seismic event in a day, especially annual peak of 2,100 in 2003. Then the number of seismic activity has declined since 2006 to the background level in 1999-2001. According to the typical frequency of volcanic earthquakes in the Baegdusan volcano, the frequency distribution of typical volcanic earthquakes between 2002 and 2005 indicates that all the main frequency of the earthquakes basically falls down less than 5 Hz and 5-10 Hz. These events are all the VT-B and LP events caused by the shallow localized fracture and intrusion of magma. The horizontal displacement measurement by GPS during the period from 2000 to 2007 of the Baegdusan stratovolcano area indicates that an inflated process has been centered at the summit caldera since 2002. The displacement between 2002 and 2003 reached at a maximum value of 4 cm. After 2003, the deformation rate of the volcano continued to decrease with unusual variation during the period from 2006 to 2007. After 2003 the vertical displacement uplift rate falls down gradually but still keeps in an uplift trend in northern slope. It is generally believed that when $^3He/^4He(R)$ in a gas sample from a hot spring exceeds $^3He/^4He(R)$ in the atmosphere, it can be concluded that mantle-source. And temperatures of hot springs are rising steadily to $83^{\circ}C$. It is unrest signals at the Baegdusan, which is potentially active. The Baegdusan volcano is now in unrest status, there is eruption threat in the near future. Intensified monitoring and emergency response plan for volcanic risk mitigation are urgent for the volcano.
Quercus acutissima ectomycorrhizae were classified as apical type, linear type, clavate type, diffuse type, pyramidal type, coralloid type, and nodular type. The surface texture of the fungal mantle at the initial stage of mycorrhizal formation was velvety. The surface texture of Pisolithus tinctorius(Pt) mycorrhizae at 30 September was well-developed felty mantle, Yellowish white. Except Pt all mycorrhizae formed by ectomycorrhizal fungi used to experiment were white with felty mantle. Mycorrhizae at the initial stage of mycorrhizae formation were creamy or creamy brown and swelled with thin mantle. Transverse and longitudinal sections showed radially-elongated cortical cell layers and epidermal cell with Hartig net. The transversal wideth of radially-elongated cortical and epidermal cells in the mycorrhizae with thick mantle on 30 September did not different with the mycorrhizae with thin mantle on the initial stage. Pt #250 formed coralloid mycorrizae but Pt KJ-1 did not although they are same species. On the mean length of linear type ectomycorrhizae of Pt KJ-1(2.21mm) was 1.5 times longer than that of Pt #250(1.32mm). The total dry weight of seedlings inoculated with Pt KJ-1, Pt #250, Lycoperdon pedicellatum, Scleroderma verrucosum were significantly heavier than those of suillus granulatus, Laccaria laccata.
Park, Byung-Jun;Choi, Ju-Hyeon;Kim, Chan-Sub;Im, Geon-Jae;Oh, Byung-Youl;Shim, Jae-Han
The Korean Journal of Pesticide Science
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v.9
no.1
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pp.70-80
/
2005
To evaluate the exposure of molinate in agricultural environment and its effect against the non-target crop in air, this experiment was conducted to elucidate volatilization characteristics of molinate in aquatic condition and to determine critical concentration of molinate in the air causing phytotoxicity to Chili pepper. Cumulative volatilized rate of molinate from water was 22.7% at $35^{\circ}C$ for water temperature and 20 L/min for air velocity while 3.2% at $25^{\circ}C$ and 10 L/min within 47 hour after applied under closed system, respectively. The molinate concentrations in air above 60 cm height from soil surface of valley and open paddy rice field were reached the highest value of 18.17 and $11.59{\mu}g/m^3$, respectively within 24 hours after applying granular formulation at dose rate of molinate 150 g/1,000 $m^2$. However, their concentrations were drastically diminished to around 0.18 and $0.51{\mu}g/m^3$ level in 20 days after application, which volatilization pattern were similar to both regions. Also, the concentration of molinate in air above 60 cm height from soil surface was distributed higher 2 times than that above 180 cm height. Meanwhile, a phytotoxic symptom against the nearby chili pepper was revealed within three days after applied and molinate was detected $0.004{\sim}0.006$ mg/kg level from severe damaged leaves. The dose and exposure relations of molinate in the air against the non-target crop was also investigated in lab trial. The phytotoxic symptom, shriveled leaves, of the chili pepper was encountered by exposing two days with concentration of $13.6{\mu}g/m^3$, three days with $6.8{\mu}g/m^3$ or four days with $3.4{\mu}g/m^3$. The symptom was still recovered within four weeks after the plants had received fresh air. On the other hand, the phytotoxic response through root uptake of the herbicide in water culture was relatively insensitive, in which the symptom is observed ten days with the concentration of 300 ${\mu}g/L$.
Journal of the Korean Society of Food Science and Nutrition
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v.34
no.8
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pp.1274-1283
/
2005
The sensory acceptability, texture profile analysis and nutritional evaluation were peformed in Korean traditional mungbean pancake (MPC) and modified MPC containing squid meat and soybean to standardize the recipes for healthy fast food market potentiality. Optimal ingredient formulations were revealed as mung-bean 55$\%$, pork 13$\%$ and vegetables 32$ \%$ for traditional MPC, and pork 3$\%$, squid 42$\%$ and soybean 55$\%$ for modified MPC using response surface methodology. Flavor and hardness correlated highly with overall accept-ability rather than appearance and color of traditional MPC. Higher squid levels raised adhesiveness, springi-ness and resiliences of modified MPC, but soybean decreased these textural attributes. Protein, lipid and total calorie of modified MPC were lower than those of traditional MPC. Computed protein efficiency ratio (C-PER) and degree of gelatinization of modified MPC were superior than traditional MPC.
This paper attempts to divide the narrative into three levels and review the approach methodology to understand Princess Bari as a narrative. If the stratification of the narrative, the analysis of each levels, and the integrated approach to them are made, this can contribute to suggesting new directions and ways to understand and study Princess Bari. The story level of Princess Bari, the surface structure, is shaped by the space movement and the chronological sequential structure of the life task that started from the birth of the main character. This story shows how a woman who was denied her existence by her father as soon as she was born finds an ontological transformation and identities through a process. Especially, the journey of finding identity is mainly formed through the events that occur through the relationship with family members. This structure, which can be found in the narrative level, forms a deep structure with the oppositional paradigm of family members' conflict and reconciliation, life and death. The thought structure revealed in this story is the problem of life is the problem of family composition, and the problem of death is also the same. In response to how to look at the unified world of coexistence of life and death, this tradition group of myths makes a relationship with man and God. This story is mainly communicated in the Korean shamanistic ritual(Gut) that sent the dead to the afterlife. Although the shaman is the sender and the participants in the ritual are the receivers, the story is well known a message that does not have new information repeated in certain situations. In gut, the patrons and participants do not simply accept the narrative as a message, but accept themselves as codes for reconstructing their lives and behavior through autocommunication. By accepting the characters and events of as a homeomorphism relationship with their lives, people accept the everyday life as an integrated view of life and death, disjunction and communication, conflict and reconciliation, and the present viewpoint. It can not change the real world, but it changes the attitude of 'I' about life. And it is a change and transformation that can be achieved through personal communication like the transformation of Princess Bari into god in myth. Thus, Princess Bari shows that each meaning and function in the story level, composition level, and communication level is related to each other. In addition, the structure revealed by this narrative on three levels is also effective in revealing the collective consciousness and cultural system of the transmission group.
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