• Journal of Korean Society of Water and Wastewater
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• v.20 no.2
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• pp.281-287
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• 2006

Maintaining adequate chlorine residual is crucial in water treatment facilities, Treatment technique, newly promulgated regulation, requires sufficient disinfection in order to control more resistant microorganisms such as Viruses and Giardia lamblia. Each water treatment plant should report various water qualities including chlorine residual and disinfection by-products, thus plenty of data has been generated. Even though statistical analysis using these data are forced to investigate the status and effect of water qualities in water facilities very few researches have been performed in korea. This study performed statistical analysis of chlorine residual during three years in Korean drinking water. The average chlorine residual concentrations were 0.701mg/L, 0.738mg/L, 0.763mg/L in 2002, 2003, 2004, respectively. Monthly variations of chlorine residual was not significant. ANOVA result showed that yearly variance of chlorine residual is different in only less than $5000m^3/day$ of water treatment capacity. The statistical analysis can help government to establish new regulation with scientific basis.

• Steel and Composite Structures
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• v.37 no.3
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• pp.307-321
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• 2020

The slim-floor solution provides an efficient alternative to the classic slab-over-beam configuration due to architectural and structural benefits. Two deficiencies can be identified in the current state-of-art: (i) the technique is limited to nonseismic applications and (ii) the lack of information on moment-resisting slim-floor beam-to-column joints. In the seismic design of framed structures, continuous beam-to-column joints are required for plastic hinges to form at the ends of the beams. The present paper proposes a slim-floor technical solution capable of expanding the current application of slim-floor joints to seismic-resistant composite construction. The proposed solution relies on a moment-resisting connection with a thick end-plate and large-diameter bolts, which are used to fulfill the required strength and stiffness characteristics of continuous connections, while maintaining a reduced height of the configuration. Considering the proposed novel solution and the variety of parameters that could affect the behavior of the joint, experimental and numerical validations are compulsory. Consequently, the current paper presents the experimental and numerical investigation of two slim-floor beam-to-column joint assemblies. The results are discussed in terms of moment-rotation curves, available rotational capacity and failure modes. The study focuses on developing reliable slim-floor beam joints that are applicable to steel building frame structures located in seismic regions.

• Journal of Microbiology and Biotechnology
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• v.30 no.4
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• pp.583-590
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• 2020

Deinococcus actinosclerus BM2^T (GenBank: KT448814) is a radio-resistant bacterium that is newly isolated from the soil of a rocky hillside in Seoul. As an extremophile, D. actinosclerus BM2^T may possess anti-inflammatory properties that may be beneficial to human health. In this study, we evaluated the anti-inflammatory effects of BM2U, an aqueous extract of D. actinosclerus BM2^T, on lipopolysaccharide (LPS)-mediated inflammatory responses in RAW264.7 macrophage cells. BM2U showed antioxidant capacity, as determined by the DPPH radical scavenging (IC₅₀ = 349.3 ㎍/ml) and ORAC (IC₅₀ = 50.24 ㎍/ml) assays. At 20 ㎍/ml, BM2U induced a significant increase in heme oxygenase-1 (HO-1) expression (p < 0.05). BM2U treatment (0.2-20 ㎍/ml) significantly suppressed LPS-induced increase in the mRNA expression of proinflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 (p < 0.05). BM2U treatment also suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), which are involved in the production of inflammatory mediators. BM2U treatment also inhibited the activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs): JNK, ERK, and p-38 (p < 0.05). Collectively, BM2U exhibited anti-inflammatory potential that can be exploited in attenuating inflammatory responses.

• Journal of the Earthquake Engineering Society of Korea
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• v.9 no.3 s.43
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• pp.23-32
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• 2005

Torsional behavior of eccentric structure under seismic leading may cause the stress and/or deformation concentration, which arouse the failure of the structure in an unexpected manner. This study suggests D-R relationship which shows the overall displacement and rotation of the system based on the ultimate displacement capacity of the each lateral load resistant member. Using the suggested D-R relationship and displacement spectrum, the seismic assessment is conducted and verified in comparison with the time history analysis result. Multi-level seismic assessment Is considered which takes multiple seismic hazard levels and respective performance levels into account. Finally, based on the seismic assessment result, seismic rehabilitation process is presented. In this research, two rehabilitation methods are considered. One is done by means of stiffening/strengthening the seismic resistant members, and the other is based on the member ductility. Especially, in the first method, to optimize the rehabilitation result, the rehabilitation problem is modeled as an optimization problem, and solved using BFGS quasi-Newton optimization method.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.5-8
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• 2008

In this dissertation, experimental research was carried out to study the hysteretic behavior of reinforced high-strength concrete beam-column joints designed by high performance techniques, such as application of high-strength concrete, reducing of joint regions damage, moving of beam plastic hinge, advanced reinforcing detailings and High Ductile Fiber-Reinforced Mortar.(HDFRM) Specimens(HJCI), designed by the development of earthquake-resistant performance, moving of beam plastic hinge, and new design approach, were attained the moving of beam plastic hinge and developed significantly earthquake-resistant performance of such joints. Specimens(HJRP), designed with HDFRM, were indicated more stable hysteresis behavior, high load carrying capacity, and distributed crack pattern of specimens HJRP when compared to the control specimen.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.645-658
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• 2021

Porins are essential for the viability of Gram-negative bacteria. They ensure the uptake of nutrients, can be involved in the maintenance of outer membrane integrity and define the antibiotic or drug resistance of organisms. The function and structure of porins in proteobacteria is well described, while their function in photoautotrophic cyanobacteria has not been systematically explored. We compared the domain architecture of nine putative porins in the filamentous cyanobacterium Anabaena sp. PCC 7120 and analyzed the seven candidates with predicted OprB-domain. Single recombinant mutants of the seven genes were created and their growth capacity under different conditions was analyzed. Most of the putative porins seem to be involved in the transport of salt and copper, as respective mutants were resistant to elevated concentrations of these substances. In turn, only the mutant of alr2231 was less sensitive to elevated zinc concentrations, while mutants of alr0834, alr4741 and all4499 were resistant to high manganese concentrations. Notably the mutant of alr4550 shows a high sensitivity against harmful compounds, which is indicative for a function related to the maintenance of outer membrane integrity. Moreover, the mutant of all5191 exhibited a phenotype which suggests either a higher nitrate demand or an inefficient nitrogen fixation. The dependency of porin membrane insertion on Omp85 proteins was tested exemplarily for Alr4550, and an enhanced aggregation of Alr4550 was observed in two omp85 mutants. The comparative analysis of porin mutants suggests that the proteins in parts perform distinct functions related to envelope integrity and solute uptake.

• Wind and Structures
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• v.32 no.1
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• pp.55-69
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• 2021

Ultrapure ferritic stainless steel provides a new generation of long-span metal roof systems with continuous welding technology, which exhibits many unknown behaviors during wind excitation. This study focuses on the wind-resistant capacity of a new continuous welding stainless steel roof (CWSSR) system. Full-scale testing on the welding joints and the CWSSR system is performed under uniaxial tension and static ultimate wind uplift loadings, respectively. A finite element model is developed with mesh refinement optimization and is further validated with the testing results, which provides a reliable way of investigating the parameter effect on the wind-induced structural responses, namely, the width and thickness of the roof sheeting and welding height. Research results show that the CWSSR system has predominant wind-resistant performance and can bear an ultimate wind uplift loading of 10.4 kPa without observable failures. The welding joints achieve equivalent mechanical behaviors as those of base material is produced with the current of 65 A. Independent structural responses can be found for the roof sheeting of the CWSSR system, and the maximum displacement appears at the middle of the roof sheeting, while the maximum stress appears at the connection supports between the roof sheeting with a significant stress concentration effect. The responses of the CWSSR system are greatly influenced by the width and thickness of the roof sheeting but are less influenced by the welding height.

• Journal of the Korean GEO-environmental Society
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• v.23 no.5
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• pp.25-32
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• 2022

As recently, there have been two earthquakes with a magnitude of 5.0 or greater in Korea and the number of smaller earthquakes has increased, a lot of research and interest in earthquake-resistant design are increasing. Especially, the Pohang earthquake has also raised interest in earthquake-resistant design of port facilities. In this study, experiments and analysis were conducted on the dynamic behavior of upright and inclined breakwaters during earthquakes among port structures through the 1g shaking table test. To this end, three seismic waves were applied to the model to which the similarity law (scale effect) was applied: long period (Hachinohe), short period (Ofunato) and artificial seismic waves. The acceleration and displacement of the upright and inclined breakwaters were analyzed according to whether the DCM method was reinforced during earthquakes based on the results of shaking table test. As the result, the dynamic behavior of the upright and inclined breakwater shows a tendency to suppress the amplification of acceleration as bearing capacity and rigidity increase when DCM method is reinforced.

• Biomolecules & Therapeutics
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• v.30 no.4
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• pp.368-379
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• 2022

Hyaluronic acid (HA), a ligand of CD44, accumulates in some types of tumors and is responsible for tumor progression. The nuclear factor erythroid 2-like 2 (NRF2) regulates cytoprotective genes and drug transporters, which promotes therapy resistance in tumors. Previously, we showed that high levels of CD44 are associated with NRF2 activation in cancer stem like-cells. Herein, we demonstrate that HA production was increased in doxorubicin-resistant breast cancer MCF7 cells (MCF7-DR) via the upregulation of HA synthase-2 (HAS2). HA incubation increased NRF2, aldo-keto reductase 1C1 (AKR1C1), and multidrug resistance gene 1 (MDR1) levels. Silencing of HAS2 or CD44 suppressed NRF2 signaling in MCF7-DR, which was accompanied by increased doxorubicin sensitivity. The treatment with a HAS2 inhibitor, 4-methylumbelliferone (4-MU), decreased NRF2, AKR1C1, and MDR1 levels in MCF7-DR. Subsequently, 4-MU treatment inhibited sphere formation and doxorubicin resistance in MCF7-DR. The Cancer Genome Atlas (TCGA) data analysis across 32 types of tumors indicates the amplification of HAS2 gene is a common genetic alteration and is negatively correlated with the overall survival rate. In addition, high HAS2 mRNA levels are associated with increased NRF2 signaling and poor clinical outcome in breast cancer patients. Collectively, these indicate that HAS2 elevation contributes to chemoresistance and sphere formation capacity of drug-resistant MCF7 cells by activating CD44/NRF2 signaling, suggesting a potential benefit of HAS2 inhibition.

• Journal of The Korean Society of Agricultural Engineers
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• v.66 no.1
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• pp.39-48
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• 2024

Plastic greenhouses are simple structures consisting of lightweight materials such as steel pipes and polyvinyl chloride. However, serious damage occurs due to heavy winds and typhoon every year. To prevent a collapse of structural members, the Ministry of Agriculture and Rural Development has distributed plans and specifications for disaster-resistant standards. Despite these efforts, more than 50% of greenhouses still do not satisfy the disaster-resistant standards. Among the greenhouses that do not meet these standards, 85% are single-span greenhouses proposed 20 years ago. Consequently, there is a need to evaluate the safety of wind loads for the single-span greenhouse. Unfortunately, there are no design specifications for the greenhouses under wind loads. Therefore, a Korean design standard (KDS) has been utilized. KDS is defined with reference to wind speeds occurring once every 500 years, raising concerns about potential overdesign when considering the durability of plastic greenhouses. To address this, the modified wind load, considering the durability of the plastic greenhouse, was calculated, and a safety evaluation was conducted for sigle G-type plastic greenhouse. It was observed that the moment acting on the windward surface was substantial, and there was a risk of the foundation being pulled out if the basic wind speed exceeded 32 m/s. In terms of the combination strength ratio, it was less than 1.0 only on the leeward side when the basic wind speed was 24 m/s and 26 m/s. However, in all other cases, it exceeded 1.0, indicating an unsafe condition and highlighting the necessity for reinforcement.