• Journal of Microbiology and Biotechnology
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• v.32 no.8
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• pp.1054-1063
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• 2022

Trehalose is a non-conventional sugar with potent applications in the food, healthcare and biopharma industries. In this study, trehalose was synthesized from maltose using whole-cell Pseudomonas monteilii TBRC 1196 producing trehalose synthase (TreS) as the biocatalyst. The reaction condition was optimized using 1% Triton X-100 permeabilized cells. According to our central composite design (CCD) experiment, the optimal process was achieved at 35℃ and pH 8.0 for 24 h, resulting in the maximum trehalose yield of 51.60 g/g after 12 h using an initial cell loading of 94 g/l. Scale-up production in a lab-scale bioreactor led to the final trehalose concentration of 51.91 g/l with a yield of 51.60 g/g and productivity of 4.37 g/l/h together with 8.24 g/l glucose as a byproduct. A one-pot process integrating trehalose production and byproduct bioremoval showed 53.35% trehalose yield from 107.4 g/l after 15 h by permeabilized P. moteilii cells. The residual maltose and glucose were subsequently removed by Saccharomyces cerevisiae TBRC 12153, resulting in trehalose recovery of 99.23% with 24.85 g/l ethanol obtained as a co-product. The present work provides an integrated alternative process for trehalose production from maltose syrup in bio-industry.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.10a
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• pp.403-410
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• 1995

A loss of the residual heat removal (RHR) accident during mid-loop operation of Yong-gwang Nuclear Units 3/4 was analyzed using the RELAP5/MOD3.1.2 code. In this work the following assumptions are used; (i) initially the reactor coolant system (RCS) above the hot leg center line is filled with nitrogen gas, (ii) two 3/4-inch diameter vent valves on the reactor vessel head and the top of pressurizer in the reactor coolant system are always open, and a level indicator is connected to the RMR suction line, (iii) the two steam generators are in wet layup status and the steam generator atmospheric dump valve assemblies are removed so that the secondary side pressure remains at nearly atmospheric condition throughout the accident, and (iv) the loss of RHR is presumed to occur at 48 hours after reactor shutdown. Findings from the RELAP5 calculations are (i) the core boiling begins at ∼5 min, (ii) the peak RCS pressure is ∼3.0 bar, which implies a possibility of temporary seal break, (iii) ∼94 % of the decay heat is removed by reflux condensation in the steam generator U-tubes in spite of the presence of noncondensable gas, (iv) the core uncovery time is evaluated to be 7.2 hours. Significant mass errors were observed in the calculations.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2022.11a
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• pp.149-150
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• 2022

In Korea, there is no clearly established technology for diagnosing and evaluating the damage depth of concrete structures created by fire. There have been cases of repairing, reinforcing, and demolishing degraded structures without diagnosing the damage depth. The commonly used phenolphthalein-based carbonate thickness measurement does not satisfy the complete disappearance condition of Ca(OH)₂ in the interval where the relatively stationary flow declines after exposure to high temperatures. Even a small amount of Portlandite[Ca(OH)₂] reacts with a red color, and the damage depth is misjudged. It was confirmed by X-ray diffraction and scanning electron microscope that Ca(OH)₂ existed from 300℃ to 500℃ where the residual compressive strength decreased.

• Journal of IKEEE
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• v.28 no.2
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• pp.158-167
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• 2024

In this study, U-net model based on electrical capacitance is applied to monitor the condition inside the pipeline of semiconductor rear end process implemented in the numerical simulation. Capacitance values measured from electrodes attached to the pipeline is used as input data for the U-net network model and estimated permittivity distribution by the U-net model is used to reconstructed cross-sectional image at the pipeline. In the numerical simulation, images reconstructed by U-net model, Fully-connected neural network (FCNN) model and Newton-Raphson method are compared for evaluation. U-net model shows good results as compared to other models.

• International conference on construction engineering and project management
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• 2024.07a
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• pp.1188-1194
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• 2024

High-energy manufacturing processes, including laser welding, are actively being adopted not only in precision machinery industries but also in the shipbuilding and construction sectors. Laser welding, in particular, is gaining prominence in the industry due to its faster welding speed and reduced distortion compared to conventional arc welding methods. Integration of automated welding systems is anticipated to address challenges in shipbuilding and construction industries, which are currently facing a shortage of skilled labor. For successful implementation of automated welding systems, it is essential to predict and design for the post-welding effects, such as residual deformation and stresses. However, in the case of high-energy manufacturing like laser welding, the welding bead morphology differs from that of arc welding, and the heat load conditions applied during simulation are distinct. To facilitate accurate simulation predictions, the development of a suitable heat source for predicting welding bead morphology in high-energy manufacturing processes is crucial. The Block-dumping method is proposed for rapid simulation and on-site application, with the shape of the welding bead being imperative for its effectiveness. In this study, data on the welding bead morphology of Nickel-based steel was obtained. Using Deep Learning techniques, we successfully predicted the bead morphology and confirmed minimal discrepancies when compared to actual results. This outcome allows for the simulation of welding under untested conditions, offering practical applicability in the field. Additionally, we present a heat source model (heat load condition) to ensure a highly accurate interpretation of the results.

• Journal of the Korea institute for structural maintenance and inspection
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• v.15 no.6
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• pp.135-146
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• 2011

Steel structures have been generally painted to prevent corrosion damage. However, the painted film is deteriorated with increase in service life, and then corrosion damage resulting in cross sectional area occurs on steel surface. As a result, the buckling strength of steel structures can be decreased due to the corrosion damages. The evaluation method of the axial buckling strength of columns about a variety of section shapes and supporting conditions have been presented, but evaluation method of buckling strength about irregular nonprismatic columns is not established. In this study, the axial buckling strength of corroded steels was evaluated based on the buckling test results of corroded steel specimens that were cut off at a temporary steel structure. The corroded specimens were picked up total 10 specimens according to various slenderness ratio from the web of a temporary structure's main beam. The length of specimens is 200, 300, 400, 500 and 600mm respectively. The rust productions were removed by the chemical treatment. Then, the surface geometry was measured at intervals of $1{\times}1mm$ by using the optical 3D digitizing system, and the residual thickness of the specimens was calculated. The axial buckling test was performed on 10 corroded specimens and 12 non-corroded specimens under the fixed-fixed support condition. From the test results, the effect of corrosion damages on axial buckling load was investigated. Regardless of corrosion damage degree, the axial buckling strength of corroded specimens and non-corroded specimens was evaluated identically by using minimum average residual thickness or average residual thickness to minus its standard deviation. Reasonable measuring intervals of residual thickness was proposed by using the results to apply for practical works.

• The Korean Journal of Pesticide Science
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• v.19 no.3
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• pp.204-209
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• 2015

This study was carried out to investigate residual characteristics of insecticide acetamiprid in asparagus under greenhouse condition from July to August and consequentially to obtain basic data for guideline on safe use of this pesticide in asparagus. Residues of acetamiprid in young stem of asparagus before and after removing foliage were analyzed from samples harvested at 0, 1, 3, 5 and 7 days after single application before harvest. As a result, residues of acetamiprid in young stem of asparagus before and after removing foliage at 0 day were 0.27 mg/kg and 0.14 mg/kg, respectively, which were higher than tentative limit (0.1 mg/kg). However, 3 days later residues of acetamiprid were lower than the tentative limit, representing 0.08 mg/kg and 0.03 mg/kg in the asparagus before and after removing foliage, respectively. Acetamiprid was undetectable in both samples at 5 days since the concentrations were less than detection limit (0.02 mg/kg) in this study. In summary, the half-life of acetamiprid in asparagus regardless of removing foliage was quite short under greenhouse condition from July to August, in the range of 1-3 days, and single application of acetamiprid water dispersible granule in/on asparagus at 7 days before harvest would have no problem on safety issues about pesticide residue. This result might be basic information to construct guideline for safe use of acetamiprid in asparagus.

• The Journal of Korean Academy of Prosthodontics
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• v.45 no.4
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• pp.506-521
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• 2007

Statement of problem: The cumulative success rate of wide implant is still controversial. Some previous reports have shown high success rate, and some other reports shown high failure rate. Purpose: The aim of this study was to analyze, and compare the biomechanics in wide implant system embeded in different width of crestal bone under different occlusal forces by finite element approach. Material and methods: Three-dimensional finite element models were created based on tracing of CT image of second premolar section of mandible with one implant embedded. One standard model (6mm-crestal bone width, 4.0mm implant diameter central position) was created. Varied crestal dimension(4, 6, 8 mm), different diameter of implants(3.3, 4.0, 5.5, 6.0mm), and buccal position implant models were generated. A 100-N vertical(L1) and 30 degree oblique load from lingual(L2) and buccal(L3) direction were applied to the occlusal surface of the crown. The analysis was performed for each load by means of the ANSYS V.9.0 program. Conclusion: 1. In all cases, maximum equivalent stress that applied $30^{\circ}$ oblique load around the alveolar bone crest was larger than that of the vertical load. Especially the equivalent stress that loaded obliquely in buccal side was larger. 2. In study of implant fixture diameter, stress around alveolar bone was decreased with the increase of implant diameter. In the vertical load, as the diameter of implant increased the equivalent stress decreased, but equivalent stress increased in case of the wide implant that have a little cortical bone in the buccal side. In the lateral oblique loading condition, the diameter of implant increased the equivalent stress decreased, but in the buccal oblique load, there was not significant difference between the 5.5mm and 6.0mm as the wide diameter implant. 3. In study of alveolar bone width, equivalent stress was decreased with the increase of alveolar bone width. In the vertical and oblique loading condition, the width of alveolar bone increased 6.0mm the equivalent stress decreased. But in the oblique loading condition, there was not a difference equivalent stress at more than 6.0mm of alveolar bone width. 4. In study of insertion position of implant fixture, even though the insertion position of implant fixture move there was not a difference equivalent stress, but in the case of little cortical bone in the buccal side, value of the equivalent stress was most unfavorable. 5. In all cases, it showed high stress around the top of fixture that contact cortical bone, but there was not a portion on the bottom of fixture that concentrate highly stress and play the role of stress dispersion. These results demonstrated that obtaining the more contact from the bucco-lingual cortical bone by installing wide diameter implant plays an important role in biomechanics.

• Journal of Soil and Groundwater Environment
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• v.10 no.5
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• pp.18-24
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• 2005

Water quality changes of wastewater effluent in the shallow aquifier condition was investigated using laboratory unsaturated and saturated SAT columns for over five months. Average DOC removal was 31.9% in the unsaturated SAT column whereas no removal occurred in the saturated SAT column. Under the shallow aquifer condition, nitrification was not completed in the unsaturated SAT column, releasing residual ammonium nitrogen into the saturated SAT column. Short retention time (one day) in the shallow unsaturated SAT column rendered DO of about 2 mg/L to the influent of the saturated SAT column. Phosphate was not removed at all in the unsaturated SAT column while complete removal was achieved in the saturated column. Consequently, organic and inorganic compounds were removed under the shallow aquifer condition as effectively as was in deep aquifer, except for the release of ammonium and relatively high DO into the saturated SAT column.

• Korean Journal of Environmental Agriculture
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• v.28 no.3
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• pp.281-288
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• 2009

The strobilurin fungicides, azoxystrobin and kresoxim-methyl, were investigated to know the biological half-lives and dissipation patterns in Korean melon under plastic film house condition. Used pesticides for field application were 20% azoxystrobin of suspension concentrate and 47% kresoxim-methyl of water dispersible granule. Two pesticides were sprayed at recommended and double dose rate. Pesticide residues in Korean melon were analyzed until 14 days after application. The azoxystrobin was analyzed by HPLC equipped with UV detector after cleanup with florisil glass column. Initial residue concentrations of azoxystrobin in Korean melon at recommended and double dose rate were 0.09 mg/kg and 0.14 mg/kg, respectively. Those were less than 0.2 mg/kg maximum residue limit of Korean melon established by KFDA. The biological half-lives of azoxystrobin in Korean melon were 4.7 days at recommended dose rate and 7.8 days at double dose rate. Initial concentrations of kresoxim-methyl which was analyzed by GLC-ECD in Korean melon at recommended and double dose rate were 0.10 mg/kg and 0.23 mg/kg, respectively. Those were less than 1.0 mg/kg, MRL. The biological half-lives of kresoxim-methyl in Korean melon were 4.1 days at recommended dose rate and 4.8 days at double dose rate. The residue amounts of both pesticide was lower than MRL and biological half-lives were not so long. Because the weight of Korean melon under plastic film house condition was fast increased during cultivation.