• Journal of Food Hygiene and Safety
• /
• v.8 no.4
• /
• pp.205-214
• /
• 1993

The monoclonal antibody to chloramphenicol(CAP) was produced to develop an enzyme-linked immunosorbent assay(ELISA) for residual CAP. An immunogen(CAP-BSA) was prepared by immunogen, antibody titer was measured by indirect ELISA. Spleen cells form the immunized mouse were fused with SP2/OAg14 myeloma cells. Among hybridomas selected in HAT media, 6 clones shown high antibody titer to CAP were subjected to cloning by limit dilution, and all of the monoclonal antibodies(MCA1, 2, 3, 4, 5, 7 and 9) produced by each clone were identified as IgG1 by ELISA isotyping analysis. Competitive ELISA condition was established by using the purified monoclonal antibody MCA1 as primary antibody and CAP-HSA conjugate as coating antigen. Standard curve of CAP(n=28) showed that the lowest detection limit of CAP is 20ng/ml level. The cross-reactivities of the 6 monoclonal antibodies showed that CAP sodium succinate. CAP base, P-nitrophenol, and p-nitrobenzyl alcohol were 89∼178, 0.050∼2.237, 0.056∼0.794 and 0.013∼7.939%, respectively. No cross-reactivities were observed with phenylalanine, tyrosine, glutamine, thiamphenicol, neomycin, streptomycin, gentamicin, sulfamethazine, sulfathiazole, chlortetracycline and p-aminobenzoic acid.

• Corrosion Science and Technology
• /
• v.11 no.4
• /
• pp.151-156
• /
• 2012

The cavity formed by the ultrasonic generation in the fluid with the application of water cavitation peening collides into the metal surface. At this time, the surface modification effect such as the work hardening presents by the compressive residual stress formed due to the localized plastic deformation. In this investigation, the water cavitation peening technology in the distilled water with the lapse of time was applied to 5052-O aluminum alloy for aluminum ship of a high value. So, the optimum water cavitation peening time on the effect for surface hardening and anti-corrosion property was investigated. Consequently, the water cavitatin peening time on excellent hardness and corrosion resistance characteristic presented 3.5 min. and 5.0 min, respectively. The surface hardness in the optimum water cavitation peening time was improved approximately 45% compared to the non-WCPed condition. In addition, corrosion current density was decreased.

• Corrosion Science and Technology
• /
• v.16 no.6
• /
• pp.298-304
• /
• 2017

Coil spring steels from the automobile suspension part after field exposure for 10 years and those after anti-corrosion validation test in proving ground of 5,000 ~ 10,000 km were examined for corrosion damages. Partial loss of paint, accumulation of corrosion product, and cracking of paint and superficial material were observed. The surface and subsurface region of spring steels had compressive residual stress and high hardness by shot peening. The surface hardness values of the specimens were 620 ~ 670 Hv. They were 60 ~ 80 Hv higher than those of the samples taken from the middle part of the spring. The maximum compressive stress was -916 ~ -1208 MPa measured at depth of about $100{\mu}m$. Electrochemical impedance spectroscopy showed that the resistances of charge transfer and the paint layer of the spring steels ranged from several tens to millions ${\Omega}{\cdot}cm^2$. The resistance of the field samples was much higher than that of the proving ground samples used in this study, implying that the proving ground test condition would be more corrosive than the field environment.

• Journal of Welding and Joining
• /
• v.31 no.4
• /
• pp.73-79
• /
• 2013

Burr grinding, Tungsten Inert Gas (TIG) dressing, ultrasonic impact treatment, and peening are used to improve fatigue life in steel structures. These methods improve the fatigue life of weld joints by hardening the weld toe, by improving the bead shape, and by creating the compressive residual stress. In this study, a new post-weld treatment method improving the weld bead shape and metal structure at the welding zone using Friction Stir Processing (FSP), a welding process, is proposed to enhance fatigue life. For that, a pin-shaped tool and processing condition employing Friction Stir Processing (FSP) is established through experiments. Experimental results revealed that fatigue life is improved by around 50% compared to as-welded fatigue specimens by reducing the stress concentration at the weld toe and by generating a metal structure finer than that of flux-cored arc welding (FCAW).

• Journal of Korean Society for Atmospheric Environment
• /
• v.11 no.4
• /
• pp.361-367
• /
• 1995

This study has been carried out to investigate the filteration performance of Electrostatically Stimulated Fabric Filter(ESFF) at high temperature condition. The electric field was maintained parallel to the fabric surface. The benefits of ESFF are lower residual pressure drop, improvement of fine particle removal efficiency and increasing reduced rate of pressure drop during a filteration cycle, stable operation at higher filtering velocities. According to the variance of filtering velocities and dust loadings, the results are summarized as follows; By imposing an electric field on the filter, the reduced rate of pressure drop was 7.sim.18% at room temperature, and when filtering velocity was 1.8m/min and dust loading was 1g/m$^{3}$, the value of reduced rate of pressure drop was shown the highest. Under the electric field around the filter, the reduced rate of pressure drop was 10.sim.35% at high temperature, and when filtering velocity was 1.8m/min and dust loading was 1g/m$^{3}$, the value of reduced rate of pressure drop was shown the highest. Most of all, at high temperature, the value of reduced rate of pressure drop was resulted to 25%. Also the collecting efficiency was shown clearly improved. By the SEM photo analysis, the number of penetrated particles at the Conventional Fabric Filter was approximately two times that of Electrostatically Stimulated Fabric Filter.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.18 no.6
• /
• pp.856-862
• /
• 2008

This paper propose a advanced technique for exciting and receiving the guided torsional wave to detect flaws in pipe systems. There are some difficulties in selecting and exciting of modes by using the nickel strip attached on pipe systems, such as qualification of residual magnetic field and multi-exciting of the unwanted modes etc. In order to there difficulties we propose the new sensor, so called Crossed-coils sensor. We will prove that it is possible to select the modes to be excited and to find a optimal excitation condition for torsional mode by using the proposed sensor.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2008.10a
• /
• pp.1712-1716
• /
• 2008

Performance of ground improvement project using prefabricated vertical drains of condition, in which approximately 10m dredged fill overlies original soft foundation layer in the coastal area has been conducted. From field monitoring results, excessive ground settlement compared to predicted settlement in design stage developed during the following one year. In order to predict the final consolidation behavior, recalculation of consolidation settlements and back analysis using observed settlements were conducted. Field monitoring results of surface settlements were evaluated, and then corrected because large shear deformation was occurred by construction events in the early stages of consolidation. To predict the consolidation behavior, material functions and in-situ conditions from laboratory consolidation test were re-analyzed. Using these results, height of additional embankment is estimated to satisfy residual settlement limit and maintain an adequate ground elevation. The recalculated time-settlement curve has been compared to field monitoring results after additional surcharge was applied.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2008.10a
• /
• pp.966-973
• /
• 2008

In recent, the collapses of cut-slope is gradually increased due to the heavy rains accompanied by typhoon. Specially, many cut-slope failures and landslides was happened to Goheung, Yeosu, Suncheon region, Jeonranamdo in the middle of September 2007. The slope of investigation is width 20 m, height 22 m, and the circular failure was occurred. The parent rocks of the slope are pyroclastic rock, namely andesite, andesitic tuff et al. and the weathering grade is completely weathered to residual soils owing to rapid weathering process and has the existence of fault zone and mafic dyke. Also, lots of extension cracks are presented and the hydrologic condition is very deteriorated. As a result of the limit equilibrium analysis, the safety factor is 1.09(in dry season) and 0.64(in wet season). For the stabilization of the cut-slope, we decided to use the retaining wall, anchors and drainage apparatus.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.26 no.4
• /
• pp.28-34
• /
• 2012

Installing surge protection devices for a low-voltage system is important to ensure the survival of electric or electronic devices and systems. If surge protection devices (SPD) are installed without consideration of the concept of lightning protection zones, the equipment to be protected might be damaged despite the correct energy coordination of SPDs. This damage is induced by the reflection phenomena on the cable connecting an external SPD and the load protected. These reflection phenomena depend on the characteristics of the output of the external SPD, the input of the loads, and the cables between the load and the external SPD. Therefore, the SPD has an effective protection distance under the condition of the specific load and the specific voltage protection level of SPD. In this paper, PSCAD/EMTDC software is used to simulate the residual voltage characteristics of SPD Entering the low-voltage device. And by applying a certain voltage level, the effective protection distances of SPD were analyzed according to the each load and length of connecting cable, and the effectiveness of SPD were verified.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.23 no.2
• /
• pp.131-137
• /
• 2009

This presents the equivalent circuit model of solar cell with irradiation and temperature condition. Based on solar cell model, the photovoltaic module specification of manufacturer compare with photovoltaic module simulation and is investigated by simulation results. The obtained results indicate that residual of simulation value and specification value about photovoltaic module is lower. There is considerable validity in simulation of photovoltaic module. Thus, the optimum simulation of photovoltaic module array are studied in this paper. This paper propose the sizing optimization of photovoltaic module array for stand-alone photovoltaic system. Also, the proposed stand-alone photovoltaic system is setting in special region(in seoul). This paper presents simulation characteristic of optimization output power in seoul.