• Osong Public Health and Research Perspectives
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• 제9권6호
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• pp.334-339
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• 2018

Objectives: Human rhinoviruses (HRVs) are the major cause of the common cold. Currently there is no registered, clinically effective, antiviral chemotherapeutic agent to treat diseases caused by HRVs. In this study, the antiviral activity of dexamethasone (DEX) against HRV1B was examined. Methods: The anti-HRV1B activity of DEX was assessed by sulforhodamine B assay in HeLa cells, and by RT-PCR in the lungs of HRV1B-infected mice. Histological evaluation of HRV1B-infected lungs was performed and a histological score was given. Anti-HRV1B activity of DEX via the glucocorticoid receptor (GCR)-dependent autophagy activation was assessed by blocking with chloroquine diphosphate salt or bafilomycin A1 treatment. Results: In HRV1B-infected HeLa cells, treatment with DEX in a dose-dependent manner, resulted in a cell viability of > 70% indicating that HRV1B viral replication was reduced by DEX treatment. HRV1B infected mice treated with DEX, had evidence of reduced inflammation and a moderate histological score. DEX treatment showed antiviral activity against HRV1B via GCR-dependent autophagy activation. Conclusion: This study demonstrated that DEX treatment showed anti-HRV1B activity via GCR-dependent autophagy activation in HeLa cells and HRV1B infected mice. Further investigation assessing the development of topical formulations may enable the development of improved DEX effectiveness.

• 유기물자원화
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• 제14권4호
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• pp.121-131
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• 2006

본 연구는 산란계에서 남은 음식물과 녹차 부산물이 산란계의 산란성적과 계란품질에 미치는 영향을 연구하였다. 공시동물은 50주령 Tetran Brown종 산란계 210수를 7처리 5반복 (반복당 6수)으로 임의 배치하였다. 시험설계는 대조구와 옥수수 곡류와 대두박을 대용한 건조된 남은음식물 10, 20, 30 및 40% 첨가구 (10% DLF, 20% DLF, 30% DLF 및 40% DLF)와 1.0%의 녹차 부산물 첨가구 (1.0% GTB)와 30% DLF와 1.0% GTB를 첨가한 혼합 첨가구를 두어 사양실험을 8주 동안 실시하였다. 산란율은 대조구에 비하여 10, 20 및 40% DLF 처리구에서 유의적으로 증가하였다 (P<0.05). 난중은 대조구에 비하여 10% DLF 처리구에서 유의적으로 감소하였다 (P<0.05). 사료 섭취량은 대조구보다 20% DLF 처리구와 30% DLF와 1.0% GTB의 혼합 첨가구에서 높게 나타났다 (P<0.05). 사료 요구율은 대조구에 비하여 10% DLF 처리구와 1.0% GTB 처리구에서 유의적으로 감소하였다 (P<0.05). 난황 콜레스테롤은 DLF 처리구와 대조구 사이에서 변화가 없었다 (P>0.05). 난황의 리놀렌산은 DLF 처리구와 1.0% GTB 첨가한 처리구에서 유의적으로 증가하였다.

• Journal of Animal Science and Technology
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• 제44권4호
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• pp.419-426
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• 2002

본 시험은 성장단계별로 서로 다른 수준의 CrP를 첨가하여 성장성적, 혈액성상 및 도체품질에 미치는 영향을 규명하고자 실시하였다. 시험Ⅰ에서 일당증체, 일당건물섭취량 및 사료효율에는 유의적인 차이가 없었다(p>0.05). 혈액성상에서 혈장 Glucose와 PUN의 농도에는 영향이 없었지만 혈중 인슐린의 농도는 0.05% CrP첨가구가 대조구의 2배되는 1.47ng/ml 인데 반하여 혈중 NEFA의 농도는 59.00 mEq/dl 로 유의적으로 낮게 나타났다(p<0.05). 이는 인슐린의 농도의 증가 및 인슐린의 활성의 증가로 인하여 지방합성대사가 활발히 진행된 결과로 사료된다. 이러한 결과를 도체분석결과가 뒤받침 해주고 있는데 비록 통계적인 유의차는 없지만 0.05% CrP 첨가구의 등지방두께가 대조구에 비해 22.33% 높은 3.67cm로 나타났다. 시험Ⅱ에서 비록 일당증체, 일당건물섭취량 및 사료효율에는 유의적인 차이가 없었지만(p>0.05), 첨가초기 즉 0.05% CrP 첨가구에서는 첫 60일간, 0.025% CrP 첨가구에서는 첫 120일간 대조구보다 우수한 성적을 나타냈다. 혈장 Glucose와 PUN의 농도에는 영향이 없었다. 통계적인 유의차는 없었지만 혈 중 인슐린의 농도는 0.025% CrP 첨가구는 대조구보다 8.88% 높은 1.35ng/ml로 나타났고 0.05% CrP 첨가구는 오히려 1.13ng/ml로 대조구보다 낮게 나타났다. NEFA의 농도는 시험Ⅰ에서와 같은 차이는 없었지만 CrP 첨가수준이 증가할수록 감소하는 추세를 나타냈고 등지방두께는 일정한 변화추세를 보이지 않았다.

• 한국정보과학회논문지:데이타베이스
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• 제31권5호
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• pp.457-467
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• 2004

데이타 캐쉬를 관리하는 알고리즘의 효율성은 클라이언트에 데이타 캐쉬가 있는 시스템의 성능에 매우 큰 영향을 미친다. 클라이언트에 데이타 캐쉬가 있는 시스템에서는 서버에 추가 스페이스 부담없이 한 데이타에 대해서 두 개의 버전을 유지 관리할 수 있다. 그 이유는 하나의 데이타가 동시에 서버의 버퍼와 클라이언트의 캐쉬에 저장될 수 있기 때문이다. 본 논문에서는 버전 기법을 활용하는 캐쉬 일관성 알고리즘인 Two Versions-Callback Locking(2V-CBL)을 제안하고, 실험을 통해서 2V-CBL라 기존에 가장 높은 성능을 보였던 Asynchronous Avoidance-based Cache Consistency(AACC)의 성능을 비교하였다. 실험 결과에 의하면 2V-CBL은 최소한 AACC와 같은 성능을 보이며 일부의 트랜잭션이 데이타를 수정하지 않고 읽기만 하는 경우에는 AACC 보다 훨씬 높은 성능을 보인다.

• Journal of Microbiology
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• 제45권2호
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• pp.133-138
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• 2007

This study addresses the susceptibility of Spodoptera frugiperda (Sf9 and Sf21), Trichoplusia ni (Hi5), and S. exigua (Se301) cells to the Bombyx mori nucleopolyhedrovirus (BmNPV). Although these cells have classically been considered nonpermissive to BmNPV, the cytopathic effect, an increase in viral yield, and viral DNA synthesis by BmNPV were observed in Sf9, Sf21, and Hi5 cells, but not in Se301 cells. Very late gene expression by BmNPV in these cell lines was also detected via ${\beta}-galactosidase$ expression under the control of the polyhedrin promoter. Sf9 cells were most susceptible to BmNPV in all respects, followed by Sf21 and Hi5 cells in decreasing order, while the Se301 cells evidenced no distinct viral replication. This particular difference in viral susceptibility in each of the cell lines can be utilized for our understanding of the mechanisms underlying the host specificity of NPVs.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.75.2-75
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• 2003

To develop an effective protection strategy against tomato bushy stunt virus (TBSV), tobacco plants expressing single-chain Fv antibodies (scFv), were established. A previous had shown that the replication activity of viral replicase was inhibited by the selected scFvs. Moreover, no systemic symptom was found after virus inoculation on leaves of wt N. benthamiana infiltrated with an Agrobacterium suspension resulting i3l expression of the scFvs. However, control plants showed systemic symptoms. In this study the localization of the scFvs within two transgenic plant lines, (CP28H3, CP-P55) was demonstrated using immunogold labelling. The gold particles, indicating the presence of scFv, were mostly found In the cytoplasm of the plant cells including chloroplasts and in the cell walls. However, they were hardly found in the vacuole, nucleoplasm and intercellular spaces. Gold particles often accumulated in either the cytosol or chloroplasts showing a specific labeling, There was no difference in type of gold labeling between both transgenic lines. The localization of the scFv in the cytoplasm further conforms the inhibition of the RNA-dependent RNA polymerase (RdRp) by the selected scFv because it is known that the RdRp is localized to membraneous cytosolic structures.

• 대한음성학회지:말소리
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• 제57호
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• pp.175-189
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• 2006

Because a packet loss brings about degradation of speech quality, VoIP speech coders have PLC (Packet Loss Concealment) mechanism. G.711, which is a mandatory VoIP speech coder, also has the PLC algorithm based on pitch period replication. However, it is not robust to burst losses. Thus, we propose two methods to improve the performance of the original PLC algorithm in G.711. One adaptively utilizes voiced/unvoiced information of adjacent good frames regarding to the current lost frame. The other is based on adaptive gain control according to energy variation across the frames. We evaluate the performance of the proposed PLC algorithm by measuring a PESQ value under different random and burst packet loss simulating conditions. It is shown from the experiments that the performance of the proposed PLC algorithm outperforms that of PLC employed in ITU-T Recommendation G.711.

• Journal of Pharmaceutical Investigation
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• 제32권4호
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• pp.285-290
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• 2002

In order to assay the efficacy of newly synthesized antiviral compounds, in vitro studies of their active intracellular phosphorylated metabolites were established as compared with Zidovudine (ZDV). Antiviral base analogs require intracellular phosphorylation prior to the inhibition of HIV replication. Therefore, antiviral drugs concentrations in plasma have not reflected any direct relationship with activity or toxicity. A method has been developed to measure the concentration of total phosphorylated metabolites inside peripheral blood mononuclear cells using modified commercial radioimmunoassay (RIA). ZDV 5'-monophosphate was synthesized and used as a procedural control for RIA modification. PBMCs were isolated from whole blood and incubated with ZDV for 20 h to allow metabolic phosphorylation. Viable cells were extracted overnight with 60% methanol. After evaporation, the extract was reconstituted in Tris buffer. Samples were split into two fractions, one of which was treated with alkaline phosphatase (AP) to liberate phosphate groups. Concentrations of phosphorylated metabolites were determined by subtracting thε concentration of non-AP-treated fraction from that of the treated fraction. Recovery of phosphorylated ZDV from cell extracts was approximately 90%, and reproducibility was acceptable (coefficients of variation <15% for concentrations${\geq}$0.25 ng/mL). Intracellular concentrations $(0.135{\sim}5.019\;nmole/10^6\;cells)$ followed a nonlinear dose-response relationship over the range $0.015{\sim}2.996mM$ extracellular ZDV, with concentration-dependant saturation.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.334-334
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• 2006

Helical polymers like polyisocyanates with single screw sense are essential to exhibit sophisticated functions like molecular recognition, self-replication, chirality memory and catalytic activity. One approach that has not been explored is the effect on handedness of the polyisocyanates through end-capping with a chiral residue. Induction of chirality in poly(n-hexyl isocyanate) was studied by end-capping with chiral (R and S) 2-bromo-3-methylbutyryl chloride(R-BMBC and S-BMBC). We have shown that a control over living anionic polymerization of HIC by using a suitable initiator affords an opportunity to introduce chiral end-groups with 100% yield and in high purity. This has resulted in helicity induction through extended lengths several orders of magnitude.

• 미생물학회지
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• 제32권1호
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• pp.65-69
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• 1994

Synechococcus sp.의 cyanophage는 early, late viral function 모두 빛을 필요로 하는 것으로 나타났으며 증식 초기 2시간 동안 암처리한 경우 200%의 burst size를 나타내었다. Dark 상태에서의 Synechococcus sp.의 cyanophage증식은 대조군에 비해 11%의 burst size를 나타내었다. 광합성 억제제인 DCMU를 $10^{-6}$M로 처리했을 때 virus yield가 2%로 감소했으며 $10^{-4}$M의 CCCP는 cyanophage의 증식을 거의 중지시켰다. 또한 이러한 숙주세포의 광합성 의존도는 LPP-1, N-1과 AS-1보다는 크나 SM-1보다는 작은 것으로 나타났다.