• Journal of Microbiology and Biotechnology
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• 제28권2호
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• pp.338-346
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• 2018

Two molecular epidemiologic methods, IS6110 restriction fragment length polymorphism (IS6110-RFLP) and 24-locus mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR), are used worldwide in studies of Mycobacterium tuberculosis (MTB). Conversely, because of its poor resolution, pulsed-field gel electrophoresis (PFGE) is not widely used for MTB. In this study, we improved the 24-locus MIRU-VNTR and PFGE protocols and compared the effectiveness of these approaches for the molecular typing of MTB using 75 clinical isolates obtained from a cohort investigation of high-risk populations infected with MTB. The 24-locus MIRU-VNTR method demonstrated superior discriminatory ability, followed by PFGE and IS6110-RFLP. Next, we analyzed six isolates with clear epidemiologic connections; that is, isolates from patients who attended the same school. IS6110-RFLP and PFGE identified these samples as the same type. By contrast, according to MIRU-VNTR, two isolates differed from four other isolates at one locus each; one isolate was identified as Mtub29 and the other as QUB-26. In summary, the 24-locus MIRU-VNTR assay was the most useful molecular typing method among the three methods investigated due to its discriminatory power, short time required, and availability as an epidemiologic investigation tool. PFGE was the second-best method. Compared with the other loci assessed in the 24-locus MIRU-VNTR assay, the Mtub29 and QUB-26 loci appeared to exhibit greater variability during transmission.

• 한국세라믹학회지
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• 제41권4호
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• pp.302-312
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• 2004

메톡시폴리에틸렌글리콜모노메타크릴레이트(MPEGMAA)와 메타크릴산(MAA)의 몰비와 MPEGMAA의 폴리에틸렌옥사이드(PEO)의 길이를 변화시켜 합성한 폴리카복실레이트계 고유동화제(PCA)를 시멘트 모르타르에 첨가한 경우. 그 물성에 미치는 영향에 대하여 조사하였다. PEO의 길이가 긴 경우 ［MPEGMAA］/［MAA］ 몰비가 낮을수록 모르타르의 유동성은 향상되었다. PCA를 첨가했을 때 응결시간은 지연되며, PEO의 길이가 짧고 ［MPEGMAA］/［MAA］ 몰비가 낮을수록 응결은 더 지연되었다. 특히 MPEGMAA의 분자량이 475인 경우(에틸렌옥사이드 반복단위는 10이다) 응결시간의 지연이 가장 컸다. PCA 분자내 존재하는 PEO의 영향으로 표면장력은 크게 낮아지며, 몰탈의 기공크기분포를 크게 변화시쳤다. PCA에 의해 생성되는 기공은 대부분 7∼370$\mu\textrm{m}$ 범위의 거대 기공이며, 이 기공들에 의해 몰탈의 밀도와 압축강도가 낮아지게 되었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 1999년도 제17회 학술발표회 논문개요집
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• pp.204-204
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• 1999

The plasma source ion implantation(PSII) technique which is a method using high negative voltage pulse in plasma system has the potential to change the surface properties of polymer. PSII technique increase the surface free energy by introducing polar functional groups on the surface so that it improves reactivity, hydrophilicity, adhension, biocompatability, etc. However, the mobility of polymer chains enables the modified surface layers to adapt their composition to interfacial force. This hydrophobic recovery interrupts the stability of modified surfaces to keep for the long time. In this study, poly(methyl methacrylate)(PMMA), poly(2-hydroxyethyl methacrylate)(PHEMA), and polu(2-hydroxypropyl methacylate)(PHPMA) for contact lens application, were modified to improve the wettability with PSII technique and were investigated the surface stabilities. Polymer film was prepared with solution casting(3 wt.% solution) and was annealed at 11$0^{\circ}C$ under vacuum oven to remove solvent completely and to eliminate physical ageing. The thickness of the film measured by scanning electron microscopy (SEM) and surface profilometer was about 10${\mu}{\textrm}{m}$. Polymers were treated with different kinds of gases, pulse frequency, pulse with, pulse voltage, and treatment time. Even though PMMA, PHEMA, and PHPMA have similar repeat unit structure, the optimal treatment conditions and the tendency to hydrophobic recovery were different. PHPMA, more hydrophilic polymer than PMMA and PHEMA showd better wettability and stability after mild treatment. Surface tensions were obtained by water and diiodomethane contact angle measurements to monitor the relation between hydrophobic recovery and polymer structure. Different ion species in plasma change the polar component and dispersion component of polymer surface. For better wettability surface, the increase of polar component was a dominant factor. We also characterized modified polymer surfaces using x-ray photoelectron spectroscopy(XPS), secondary ion mass spectrometry(SIMS), Fourier Transform infrared spectroscopy(FT-IR), and SEM.

• 한국콘텐츠학회논문지
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• 제2권4호
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• pp.1-8
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• 2002

본 논문에서는 디지털 컨텐츠 저작에 있어 멀티미디어 개념단위를 설정하여 사용자의 필요에 따라 임의로 분기할 수 있도록 설계함으로써 그 학습효과를 개선하는 새로운 분기방법을 제안한다. 이 분기방법은 임의시간에 사용자의 간단한 인터페이스에 의하여 컨텐츠페이지의 일부나 여러 컨텐츠페이지에 걸쳐 존재하는 개념단위 오브젝트의 시점으로 분기ㆍ접속하여 이를 반복, 재생, 환원 등 자유롭게 재생함으로써 디지털 컨텐츠의 상호작용을 크게 향상시킨다. 이와 같은 분기방법에 의하여 접속되는 개념단위 오브젝트는 모든 종류의 디지털 컨텐츠 저작에 적용될 수 있으나, 구조도나 모델의 각 블록, 난해한 수식 등 하나의 개념적인 단위로 독립시켜 설정할 수 있는 부분을 다수 포함하는 수학적 기반의 이공계 교과목 컨텐츠나 암기단위를 다수 포함하는 언어 교육용 컨텐츠 등에 특히 효과적이다. 또한, 본 논문의 새로운 분기방법을 기존의 페이지별 혹은 목차별 분기방법과 함께 구현하면, 사용자의 상호작용을 극대화하여 컨텐츠의 학습효과를 크게 향상시킨다

• 한국산업융합학회 논문집
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• 제8권1호
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• pp.11-17
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• 2005

This study was performed to analysis the operation process when fabric pattern design was done by the use of CAD system compared with the manual work in order to determine the operation efficiency with the application of fabric design CAD system. The results of the study were as follows: 1. since 160,000 colors were supported by CAD system, color proposed by consumer was able to match exactly according to the its design. However, exact color matching was not possible by manual work. 2. Woven state of back of pattern design could be identified simultaneously with face of it for CAD system, while face and back of the fabrics should be designed separatedly in case of manual work. 3. Since the combination of warp and filling yarn was compatible with the fabric density in one repeat unit in CAD system, exact size of pattern design to be woven was able to expressed. 4. Only simple graphical expression by manual work was seen, while with the CAD system, texture and shade effect as well as graphical expression could be expressed and so fault could be reduced in advance with the simulation of actual feeling of fabrics in the screen. In conclusion, when CAD system will be introduced to the textile industry, operation time of designing pattern can be reduced. Since the operation is easy and simple, a beginner can operate CAD system easily. Thus, production and wage costs can be saved and this can be related directly with the improvement of productivity which is the main purpose of introducing CAD system.

• Mycobiology
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• 제29권3호
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• pp.121-131
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• 2001

This study was carried out to identify the phylogenetic relationships among several caterpillar fungi by comparing the sequences of internal transcribed spacer regions(ITS1 and ITS2) and 5.8S ribosomal DNA(rDNA) repeat unit. The sequences of ITS1, ITS2, and the 5.8S rDNA from 10 strains of Cordyceps species, 12 strains of Paecilomyces, 3 strains of Beauveria, 2 strains of Metarhizium and 1 strains of Hirsutella were amplified, determined and compared with the previously known Cordyceps species. The sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites could be found. In the phylogenetic tree, the species generally divided into three clusters, supported by their morphology and/or host ranges. The 5.8S rDNA and TTS1 sequences among 10 species of Cordyceps militaris were identical and only one base pair in ITS2 sequence was different. Cordyceps sinensis and Cordyceps ophioglossoides were also clearly different, although they belonged to the same cluster. The Geniank database search of species revealed sister taxa of an entomogenous fungus. Metarhizium was used as an putgroup in all taxa.

• 영어어문교육
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• 제8권2호
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• pp.105-115
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• 2003

The four skills of a language are basically required for a communication. They are very important for a learner to develop the balanced language acquisition. Today both listening and speaking skills are emphasized in the global era rather than reading and writing proficiencies. The reason is really why the learners' communicative competence is more needed than the accurate knowledge of a structure in the language. For this reason, the listening comprehension should be taught effectively using the following strategies. First, the sound difference of a language must be taught. Language is a complicated process to convey the comprehensive meaning combined with the internal and external factors of a language. In other words, the meaning for the sound of language should be transmitted by the unit of vocabulary and syntax. Second, a good listening comprehension requires the familiarity and much experience with a lot of English words to understand English sentences unconsciously. Third, as understanding the structure of language is effective for the listening comprehension, the better listening comprehension can be possible through the meaningful exercise. Fourth, the compound process of listening comprehension requires the comprehensive understanding of language, but not the separate understanding of language. Fifth, the appropriate application of the multimedia courseware helps improve the listening comprehension better than that of the existing audio, video, tape recorder and so on. Using multimedia courseware is useful as follows: A learner is able to take as much lesson as he/she wants. It does take little time to repeat about what he/she takes a lesson. It gives the lively picture with the native speakers' voices. It gives him/her(a learner) a feedback effect continuously through the interaction of computer. It controls his/her lesson in accordance with the level of a learner.

• 생명과학회지
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• 제13권4호
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• pp.400-411
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• 2003

5.8S rDNA를 포함한 ITS부위에 대한 염기서열 분석결과, 종에 따라 다양한 염기서열을 가지고 있어 분류에 이용될 수 있었으며, 특히 ITS2부위보다 ITS1부위에서 종에 대한 변이율이 높은 것으로 나타났다. 아울러 균종에 따라 정도의 차이는 있으나 사용된 모든 종들이 서로 계통분류학적 거리가 멀어서 종간의 구분이 명확하게 나타났다. P. tenuipes, I. japonica, P. japonicus는 multiple alignment분석에서 매우 유사한 염기서열을 가지고 있어, 이들 세종은 같은 종이지만 다른 이름으로 불리고 있는 것으로 나타났으며, 아울러 Paecilomyces sp. KACC 40220과 KACC 40656도 동일한 염기서열을 가지고 있어 p. tenuipes로 판단된다. 국내에서 유통되는 동충하초제품 35건과 중국산 1건에 대해 실험한 결과 23건은 P. tenuipes / japonica로, 11건은 C. militaris로, 1건은 B. bassiana로 분류되었으며, 중국산 제품 1건은 C. multiaxialis로 분류되었다.

• 한국지반공학회논문집
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• 제24권4호
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• pp.37-46
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• 2008

포장재료의 역학적 특성을 대표하는 회복탄성계수는 '86 AASHTO 설계법에서 노상의 기본입력 물성치로 적용한 이후 포장 설계, 해석뿐 아니라 포장 평가, 유지관리 등에도 보편적 개념으로 정착되어 가고 있다. 국내 노상토의 회복탄성계수는 함수비와 건조단위중량으로 대표되는 재료적 요인과 축차응력 및 구속응력에 대단히 큰 영향을 받고 있고, 하중주파수의 영향은 있으나 그 정도가 크지 않고, 하중반복횟수의 영향은 무시 가능한 것으로 알려져 있다(건설교통부 2003). 따라서 국내 포장재료에 따른 회복탄성계수의 적절한 평가는 국내 포장구조체의 역학거동 및 공용성 평가를 위해 매우 중요한 사항이다. 이와 같은 배경으로 본 연구에서는 국내 노상토 재료의 역학적 특성 변화를 포장 단면설계에 합리적으로 반영하기 위한 노상토 해석모델을 제안하였고, 제안된 모델을 적용한 유한요소해석 결과와 시험도로 실측치와의 비교 검증을 통해 제안된 모델의 신뢰성을 검증하였다.

• Journal of Chest Surgery
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• 제56권6호
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• pp.374-386
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• 2023

Background: The heightened morbidity and mortality associated with repeat cardiac surgery are well documented. Redo median sternotomy (MS) and minimally invasive valve surgery are options for patients with prior cardiac surgery who require mitral valve surgery (MVS). We conducted a systematic review and meta-analysis comparing the outcomes of redo MS and minimally invasive MVS (MIMVS) in this population. Methods: We searched PubMed, EMBASE, and Scopus for studies comparing outcomes of redo MS and MIMVS for MVS. To calculate risk ratios (RRs) for binary outcomes and weighted mean differences (MDs) for continuous data, we employed a random-effects model. Results: We included 12 retrospective observational studies, comprising 4157 participants (675 for MIMVS; 3482 for redo MS). Reductions in mortality (RR, 0.54; 95% confidence interval [CI], 0.37-0.80), length of hospital stay (MD, -4.23; 95% CI, -5.77 to -2.68), length of intensive care unit (ICU) stay (MD, -2.02; 95% CI, -3.17 to -0.88), and new-onset acute kidney injury (AKI) risk (odds ratio, 0.34; 95% CI, 0.19 to 0.61) were statistically significant and favored MIMVS (p<0.05). No significant differences were observed in aortic cross-clamp time, cardiopulmonary bypass time, or risk of perioperative stroke, new-onset atrial fibrillation, surgical site infection, or reoperation for bleeding (p>0.05). Conclusion: The current literature, which primarily consists of retrospective comparisons, underscores certain benefits of MIMVS over redo MS. These include decreased mortality, shorter hospital and ICU stays, and reduced AKI risk. Given the lack of high-quality evidence, prospective randomized control trials with adequate power are necessary to investigate long-term outcomes.