• Membrane Journal
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• v.27 no.5
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• pp.441-448
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• 2017

In this study, the effects of membrane characteristics on the power generation performance in reverse electrodialysis (RED) have been investigated with pore-filled ion-exchange membranes (PFIEMs) prepared by employing a porous polyethylene substrate and the mixtures of three cross-linking agents. As a result, it was confirmed through the correlation analyses that the cross-linking degree and free volume of the PFIEMs were effectively controlled by mixing the cross-linking agents having different molecular sizes, influencing complexly the electrochemical characteristics of the membranes and the power generation performance in RED. In particular, the pore-filled cation-exchange membranes at the optimum cross-linking conditions exhibited the power generation performance superior to that of the commercial membranes and the pore-filled anion-exchange membranes also showed the excellent performance close to that of the commercial membrane.

• Applied Microscopy
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• v.23 no.1
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• pp.15-24
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• 1993

The toxic effects of methylmercury on the ultrastructures of the brain and gill tissues of fightingfish and compensative effects of red ginseng were investigated by means of electron microscopy. The brain neuron of methylmercury exposure group showed dilatation of dendrite and axon, numerical decrease of ribosomes, partial loss of nucleoplasm and cytoplasm and considerable swelling of mitochondria as compared with the normal neuron. And necrotic cell with ruptured nucleus and vacuolated mitochondria was noticeable. While, slight swelling of mitochondria, some dilation of dendrite and axon and numerical increase of ribosome occurred in the neuron of methylmercury-red ginseng treatment group as compared with the methylmercury exposure group. In the gill lamella of methylmercury exposure group, collapse of pillar cells and arms, dilated epithelial cell and thickened membrane were observed. While, in the gill lamella of methylmercury-red ginseng treatment group, arms were slightly disintegrated and basement membrane was some thickend as compared with the methylmercury exposure group. From the above results, it is concluded that red ginseng has detoxication effect on methylmercury toxicity and so takes compensative effect on injured tissues caused by methylmercury intoxication.

• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.320-324
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• 2002

To establish a rapidly immunochemical identification on a dinoflagellate, Cochlodinium polykrikoides, a specific antigenic protein as a maker on the cell membrane was isolated. The cell membranes of C. polykrikoides and Gymnodinium sangineum were harvested by centrifugation after osmotic shock. The membrane proteins of both cells were solubilized in 50 mM Na-carbonate contained 1 mM DTT, and separated the proteins on SDS-PACE. Immune-blot on the solubilized membrane proteins of the both cells was performed with antiserum against the solubilized membrane proteins of C. polykrikoides. A 120 kDa membrane protein of C. polykrikoides had remarkablely different antigenicity from that of G. sangineum.

• Analytical Science and Technology
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• v.8 no.1
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• pp.79-84
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• 1995

Ion-selective poly(vinyl chloride) membrane electrodes for the determination of the anticholinergic drug alverine in pure substances is described. Ion-pair complex of alverine with anionic counter ion acid red 97 is investigated as electroactive compound for the electrode membrane. On the optimal composition of membrane, its slope was 55.35mV/decade, relative standard deviation was 0.61 and lower limit of linear range was $1.0{\times}10^{-5}M$. The observed potentiometric selectivity coefficient was -2.625 toward methylephedrine and -2.216 toward histidine. We can got the stable potential at pH 7.0~4.0. Response time was 20~30 seconds for solutions${\leq}10^{-4}M$, about 10 seconds for solutions ${\geq}10^{-4}M$ of these compounds.

• The Plant Pathology Journal
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• v.29 no.1
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• pp.17-30
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• 2013

Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treat-ments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.

• Journal of Ginseng Research
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• v.46 no.1
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• pp.104-114
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• 2022

Background: Abnormalities of myelin, which increases the efficiency of action potential conduction, are found in neurological disorders. Korean Red Ginseng (KRG) demonstrates therapeutic efficacy against some of these conditions, however effects on oligodendrocyte (OL)s are not well known. Here, we examined the effects of KRG-derived components on development and protection of OL-lineage cells. Methods: Primary OL precursor cell (OPC) cultures were prepared from neonatal mouse cortex. The protective efficacies of the KRG components were examined against inhibitors of mitochondrial respiratory chain activity. For in vivo function of Rb1 on myelination, after 10 days of oral gavage into adult male mice, forebrains were collected. OPC proliferation were assessed by BrdU incorporation, and differentiation and myelination were examined by qPCR, western blot and immunocytochemistry. Results: The non-saponin promoted OPC proliferation, while the saponin promoted differentiation. Both processes were mediated by AKT and extracellular regulated kinase (ERK) signaling. KRG extract, the saponin and non-saponin protected OPCs against oxidative stress, and both KRG extract and the saponin significantly increased the expression of the antioxidant enzyme. Among 11 major ginsenosides tested, Rb1 significantly increased OL membrane size in vitro. Moreover, Rb1 significantly increased myelin formation in adult mouse brain. Conclusion: All KRG components prevented OPC deaths under oxidative stress. While non-saponin promoted proliferation, saponin fraction increased differentiation and OL membrane size. Furthermore, among all the tested ginsenosides, Rb1 showed the biggest increase in the membrane size and significantly enhanced myelination in vivo. These results imply therapeutic potentials of KRG and Rb1 for myelin-related disorders.

• Textile Coloration and Finishing
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• v.9 no.1
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• pp.23-32
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• 1997

Composite membranes having different mixing ratio of Wool(SCMK) and poly(ethylene terephthalate) (PET) were prepared by dissolving wool/PET in hexafluoro-2-propanol(HFIP), casting the obtained solution on a glass plate and evaporation the solvent in the presence and absence of an electric field. The internal structure of the prepared membrane was investigated using polarise microscope dyeing and dye permeation method. In the composite membrane prepared under electric field, both components were micro mixing, while in the membranes prepared under nonelectric field, the two components formed a random sea/island structure according to different mixing ratio. Such characteristic membrane structure was influenced the permeation behavior of C.I. Acid Red 118 through the membranes from an aqueous solution.

• Membrane Journal
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• v.31 no.3
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• pp.228-240
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• 2021

The organic solvent robust polybenzimidazole (PBI) membranes were prepared as organic solvent nanofiltration (OSN) membrane for the recycling of alcoholic solvents using non-solvent induced phase separation with different dope solution concentration and coagulant composition of water/ethanol mixtures to control the membrane morphology and permeation performance. Investigation on crosslinking of polybenzimidazole indicated that the membrane crosslinked with dibromoxylene (DBX) had enough mechanical strength and solvent resistance to be applied as a OSN membranes. The crosslinked PBI membrane prepared by more than 20wt% dope concentration coagulated in water showed a rejection of > 90% to Congo Red (MW of 696.66 g/mol) while pure ethanol permeances was more than 22.5 LMH/bar at 5 bar. Investigation on coagulant composition indicated that ethanol permeance through crosslinked PBI OSN membrane increased with increasing of ethanol concentration in water/ethanol mixture coagulants.

• Bulletin of the Korean Chemical Society
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• v.27 no.9
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• pp.1439-1444
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• 2006

A $Cu^{2+}$ ion-selective membrane microelectrode has been fabricated from poly vinyl chloride (PVC) matrix membrane containing a new symmetrical hexadentate Schiff,s base 2-{1-(E)-2-((Z)-2-{(E)-2-[(Z)-1-(2-hydroxyphenyl)ethylidene]hydrazono}-1-methylpropylidene)hydrazono]ethyl}phenol (HDNOS) as a neutral carrier, Potassium tetrakis(4-chlorophenyl) borate (KTpClPB) as an anionic excluder and o-nitrophenyloctyl ether (NPOE) as a plasticizing solvent mediator. The microelectrode displays linear potential response in the concentration range of $1.0\;{\times}\;10^{-5}-1.0\;{\times}\;10^{-11}$ M of $Cu^{2+}$. The microelectrode exhibits a nice Nernstian slope of 25.9 ${\pm}$ 0.3 mV $decade^{-1}$ in the pH range of 3.1-8.1. The sensor has a relatively short response time in whole concentration ranges ($\sim$5 s). The detection limit of proposed sensor is $5.0\;{\times}\;10^{-12}$ M (320 pg/L), and it can be used over a period of eight weeks. The practical utility of the sensor has been demonstrated by using it as an indicator electrode in the potentiometric titration of $Cu^{2+}$ with EDTA. The proposed membrane electrode was used for the direct determining of $Cu^{2+}$ content in black and red pepper, and in waste water samples.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.208.2-208.2
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• 2016

Many researchers have paid attention to the studies on the interaction between non-thermal plasma and aqueous solutions for biomedical applications. The gas composition in the plasma is very important. Oxygen and nitrogen are the main gases of interest in biological applications. Especially, we focus on the oxygen concentration. In this experiment, we studied the role of oxygen concentration in plasma induced chemical reactions in solution. At first, the amount of ions are measured according to changing the oxygen concentration. And we checked the relationship between these ions and pH value. Secondly, when the oxygen concentration is changed, it identified the type and amount of radical generated by the plasma. In order to confirm the effect of these chemical property change to biological material, hemoglobin and RBCs are chosen. RBCs are one of the common basic biological cells. Thirdly, when plasma treated according to oxygen concentration in nitrogen feeding gas, oxidation of hemoglobin and RBC is checked. Finally, membrane oxidation of RBC is measured to examine the relation between hemoglobin oxidation and membrane damage through relative hemolysis and Young's modulus. Our results suggest that reactive species generated by the plasma differsdepending on the oxygen concentration changes. The pH values are decreased when oxygen concentration increased. OH decrease and NO increase are also observed. These reactive species makes change of chemical properties of solution. We also able to confirm that the difference in these reactive species to affect the oxidation of the Hb and RBCs. The Hb and RBCs are more oxidized with the high oxygen concentration conditions. But membrane is damaged more by plasma treatment with only nitrogen gas. It is shown that red blood cells membrane damage and oxidation of hemoglobin are not directly related.