• Journal of Life Science
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• v.15 no.5 s.72
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• pp.707-714
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• 2005

The conventional histochemical staining were used to study mucosubstances properties of intestinal striated border and goblet cells in four teleostean species, i. e., Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis. The following methods were used; PAS, AB pH 2.5, AB pH 1.0, AB pH 2.5-PAS, AF pH 1.7-AB pH 2.5 and HID-AB pH 2.5 stains. The mucosubstances of striated border in the proximal intestine and rectum of Sebastes schlegeli contained with neutral mucin, middle and distal intestine contained with neutral mucin and acid mucin. The striated border of all the intestines of Halichoeres poecilopterus contained with neutral mucin and acid mucin, and those of Bryzoichthys lysimus and Tnkifugu pardalis contained with neutral mucin only. The amounts of neutral mucin were moderate to considerable in Sebastes schlegeli and Halichoeres poecilopterus, minimal to small in Bryzoichthys lysimus and Tnkifugu pardalis. The amounts and properties in mucosubstances of intestinal goblet cells showed differences in species and regions. The intestinal goblet cells of Bryzoichthys lysimus, and Tnkifugu pardalis contained neutral mucin only while Sehastes schlegeli and Halichoeres poecilopterus contained mixture of neutral mucin, sulfomucin and sialomucin. The amounts of neutral mucin were considerable to large in distal intestine and rectum of Tnkifugu pardalis, while moderate to considerable in all intestines of Sehastes schlegeli, all the intestines except for middle intestine of Bryzoichthys lysimus, and proximal and middle intestine of Tnkifugu pardalis. Also it was minimal to small in middle intestine of Halichoeres poecilopterus. The intestinal goblet cells of Sehastes schlegeli contained mixture of minimal amounts of strong sulfmucin, weak sulfomucin and minimal to small amounts of sialomucin, and those of Halichoeres poecilopterus except for rectum contained mixture of minimal to small amounts of strong sulfomucin and sialomucin.

• Radiation Oncology Journal
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• v.10 no.2
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• pp.237-245
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• 1992

Five hundred and fifty patients treated for carcinoma of the uterine cervix at the Department of Therapeutic Radiology, Seoul National University Hospital from 1979 to 1986, were analyzed retrospectively for late rectal complications. Of them, 468 patients received primary radiotherapy for the cervix cancer in intact uterus, and the other eighty two patients were treated postoperatively. The cumulative incidence of radiation induced rectal complication of grade 2 or 3 was 6.7% at five years. The mean rectal dose for the group of patients with complication was higher than the group without complication, and the difference was statistically significant (p<0.01). But relationship between mean rectal dose and severity of complication was not found. The frequency of complication (grade 1, 2, 3) increased as a function of radiation dose to rectum; from 16.1% for patients with rectal dose less than 6,000 cGy to 71.2% for patients with rectal dose higher than 8,000 cGy. Among various factors analyzed, history of diabetes mellitus, number of intracavitary irradiation, size of ovoid, retroflexion of uterus and the total dose delivered to rectum turned out to have significant effect on the complication.

• Progress in Medical Physics
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• v.13 no.2
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• pp.79-84
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• 2002

In this study, a cylindrical ultrasound applicator is developed for the treatment of vagina and rectum in combination with high dose rate brachytherapy. A cylindrical transducer (PZT-8, 1=1.5 cm, thickness=1.5mm OD=2.5 cm) was used as an energy source for induction of hyperthermia. Three single-element applicators were constructed to examine the performance of the PZT material. Vector impedance was measured to determine driving frequency. The efficiencies of the elements were determined using a radiation force technique to evaluate the feasibility of using the applicator as a hyperthermia source. A multi-element ultrasound applicator was designed using the PZT-8 material for the treatment of vagina. Results from the vector impedance measurements showed maximum magnitude at 1.78, 1.77, and 1.77 MHz for applicator 1,2, and 3, respectively. The radiation force measurements showed that the acoustic power of 40 watts was obtained in all three elements. The average efficiencies of the elements were 61.4, 65.2, and 54.0% for element 1, 2, and 3, respectively. The designed ultrasound hyperthermia applicator could be used in combination with high dose rate brachytherapy for the treatment of vagina and rectum. The use of this applicator with intracavitary brachytherapy could offer improved tumor control by increasing radiosensitiyity of the tumor.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.4
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• pp.438-449
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• 1996

Development of alimentary tract and nutrient absorption in embryo of the viviparous teleost surfperch, Ditrema temmincki were investigated with histochemical methods. Just after hatching mouth of the embryo was opened, and the end part of alimentary tract was opened in TL 4.0 mm. Mucosal folds in the alimentary tract appeared at posterior region from TL 5.0 mm. In TL 30.0 mm, the alimentary tract of the embryo could be distinguished into pharynx, esophagus, anterior intestine, mid intestine, posterior intestine, rectum and anus. From over TL 50.0 mm, the internal histological patterns of the alimentary tract showed similar structures as seen in the adult. The mucous cells in the pharynx were positively reacted to PAS in TL 7.0 mm. PAS positive goblet cell appeared in the intestine from 25.0 mm, from TL 30.0 mm in the rectum, from TL 40.0 mm in the anus and from 50.0 mm in the esophagus. Yolky materials were absorbed completely in TL 6.0 mm. PAS positive polysaccharide absorptive cells began to appear at the posterior parts of the intestine in TL 7.0 mm and appeared in TL 13.0 mm at the rectum, in TL 15.0 mm at the anterior intestine and in TL 40.0 mm at the anterior part of anus. During the gestation period, nutrient absorptive type of the embryo within the maternal body is a placental analogues type of metrotrophy.

• Korean Journal of Veterinary Research
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• v.38 no.1
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• pp.107-117
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• 1998

The cultivation for development of Ascaris suum from the second-stage larvae($L_2$) embryonated egg and the third-stage of rat-derived larvae($L_3$) recovered from lung of rats were performed to use the screening test of anthelmintics in vitro. The preparations of larvae for cultivation were that the artificially-hatched $L_2$ incubated the embryonated eggs of Ascaris suum in 0.1% formalin solution at $25^{\circ}C$ for 28 days and the rat-derived larvae($L_3$) recovered from the lung of rat infected with the embryonated eggs of Ascaris suum on 7 days after infection(DAI). The cultivation for development of Ascaris suum from the embryonated eggs($L_2$) and the rat-derived larvae($L_3$) for 14 days in RPMI medium 1640(with 5% bovine calf serum) were as follows : 1. The sizes of the liberated larvae($L_2$) which were artificially hatched from embryonated eggs with glass beads(diameter 5mm) were $190{\sim}250{\mu}m$ on 1 days in culture(DIC). The second-stage larvae were molted into third-stage larvae(early $L_3$; $250{\sim}300{\mu}m$) and the features of these larvae were first observed such as cephalic cuticle, esophageal lumen and anus etc. on 5 DIC and the sizes of late third-stage larvae were $250{\sim}450{\mu}m$ on 10 DIC. The sizes of early fourth-stage larvae($L_4$) were $500{\sim}700{\mu}m$ and the features of these larvae were more pronounced in internal organs on 15 DIC. 2. The sizes of third-stage larvae($L_3$) recovered from the lung of rats were $1,340{\sim}1,370{\mu}m$ and the feartures of cephalic cuticle, esophageal lumen, intestine, rectum, anus were visualized by inverted microscope on 1 DIC. The fourth-stage larva($L_4$) completed by third ecdysis were recognizable and sizes of early fourth-stage larvae were developed as $1,400{\sim}2,200{\mu}m$ on 5 DIC. The sizes of middle fourth-stage of larva were $1,900{\sim}2,300{\mu}m$ and the thickened epithelial rectum was observed on 10 DIC. The rectum and anus of late fourth-stage larva($L_4$ $2,500{\sim}3,200{\mu}m$) had developed completely in RPMI medium 1640 on 15 DIC.

• Korean Journal of Veterinary Research
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• v.40 no.2
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• pp.247-256
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• 2000

The regional distributions and relative frequencies of endocrine cells were studied histochemically (Grimelius and Masson-Hamperl (M-H) silver methods) in the alimentary tract of the red-eared slider, Trachemys scripta elegans. Samples were taken from the esophagus, fundus, pylorus, duodenum, jejunum, ileum and large intestine. Argyrophil (Grimelius-positive) cells and argentaffin (M-H-positive) cells were found in the whole alimentary tract in this study. Spherical to spindle and/or oval to round-shaped argyrophil or argentaffin cells were located in the gastric glands of the stomach regions, in the basal portion of the epithelium of intestinal tract or the esophagus with variable frequencies. Argentaffin cells were more numerously detected in the whole alimentary tract compared with those of argyrophil cells in this study. Argyrophil cells were observed in the whole alimentary tract including the esophagus and the most predominant region was the rectum with moderate frequency. The relative frequency of these cells was rare in the esophagus, fundus, duodenum, jejunum and ileum, respectively and a few frequency in the pylorus. Argentaffin cells were also observed in the whole alimentary tract including the esophagus and the most numerously demonstrated region was the rectum with numerous frequency. They were observed with a few frequencies in the remaining regions of the alimentary tract except for the rectum, respectively. However, to know the exact type of the argyrophil cells and argentaffin cells that were observed in this study, more developmental methods such as immunohistochemistry were needed.

• Advances in pediatric surgery
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• v.8 no.2
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• pp.119-125
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• 2002

The aim of this study was to evaluate the posterior sagittal anorectoplasty (PSARP) as a re-do operation in patients who failed initial repair of anorectal malformation. Nine patients (4 boys and 5 girls) who had previous failed surgery for anorectal malformation underwent secondary operations through posterior sagittal approach. The main reasons of surgery were constipation (n=3) and persistent anatomical derangement in spite of previous correction surgery (n=6). In addition to constipation, the former group (n=3) had various anatomical defects, and the latter group (n=6), of course, had constipation in some degrees. Patients ranged in age from 2 to 19 years (median 3 years) with only one over the age of 6 years. The primary procedures included PSARP (n=8) and anoplasty (n=1). The rectum was mobilized from surrounding structures through posterior sagittal approach and anatomical defects were corrected. The rectum underwent reconstruction, which involved relocation of the rectum and anus within the limits of the intact muscle complex. Patients underwent follow-up for periods ranging from 6 to 77 months (mean 37 months) after surgery. Anatomical corrections of all the defects were successfully fulfilled in 9 patients. All the patients were satisfied with the functional results after redo-PSARP compared with the preoperative defecatory function. This study suggests that (1) some of the patients with troublesome constipation may have anatomical defects, prominent or hidden, (2) surgeons should suspect the possibility of anatomical defect as the cause of incontinence and (3) preoperative thorough investigation to reveal the anatomical defects should be included in estimating patients with severe incontinence after previous surgery and planning the correction for failed previous surgery as well.

• Journal of radiological science and technology
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• v.36 no.1
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• pp.31-38
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• 2013

The prostate cancer is the most common malignant tumor in males. Prostate cancer is the most common malignant tumor that occurs in the male in Korea in 2007 to an annual average of 5,292 cases and 3.3% of the total cancer incidence seventh occurred. Our study compared property for tomotherapy and proton therapy in radiotherapy of prostate cancer patients. We analyzed DVH(Dose Volume Histogram) and dose distribution for prostate, bladder and rectum for radiation treatment planning of prostate cancer with 11 patients in Ilsan K cancer hospital from June to November 2011. There was no differences between tomotherapy and proton therapy in the purpose of prostate cancer therapy for PTV. The adjacent organs of bladder and rectum of average dose-volume were 2port proton therapy that it was low dose treatment comparing with tomotherapy and 5port proton therapy. $H{\cdot}I$ of proton therapy was less than $H{\cdot}I$ of tomotherapy. Also, 5port was less than 2port in $H{\cdot}I$ of proton therapy. However, 2port proton therapy has more advantage over 5port proton therapy that the bladder and rectum of average dose-volume and control time of equipment in radiotherapy of prostate cancer.

• Journal of Yeungnam Medical Science
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• v.6 no.1
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• pp.87-98
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• 1989

Clinical estimation of bladder and rectal doses from high dose rate intracavitary irradiation in carcinoma of the cervix uteri has been initiated on a routine basis in an effort to obtain the optimum radiotherapeutic dose. Simulation films with contrast media are used to image the bladder and rectum, and dose rates are estimated at various interesting points with the aid of treatment planning computer, NEC Therac-2300. Fifty-three patients have been reviewed in order to ascertain the correlation between radiation dose at interesting points in the bladder and rectum and the dose at Point A and B. The dose ratio between doses at Point A 'and interesting points is an important clinical factor in evaluating the treatment planning. This also serves as documentation of the dose to normal structures within the treatment volume. Authors conclude that obtained data are within acceptable ranges and routine simulation films of the bladder and rectum after administration of contrast media with dose calculations at interesting points provide important information for optimizing radiotherapy planning in the treatment of cervical carcinoma without increased time and effort or patient's discomfort.

• Journal of the Korean Society of Radiology
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• v.16 no.6
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• pp.735-740
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• 2022

In order to reduce side effects such as rectal bleeding in the Brachytherapy, the rectal retractor and wet gauze have been used to increase the distance between the rectum and the tandem. However, there were disadvantages that it was difficult to insert through a narrow entrance into the vagina and poor reproducibility. Also, based on the CT image of the selected catheter, the distance from the tandem to the rectum with or without the balloon catheter was checked and the reduction of the dose to the rectum was confirmed. As a result of the experiment, catheter No. 7 was selected considering the maximum balloon size at a level that does not affect the distance between the start point of the balloon and the end of the catheter, and the ovoid applicator. Based on the CT image of the selected catheter, the degree of expansion according to the presence or absence of the balloon catheter was compared, and it was found that the distance difference was 0.3 - 1 cm. In addition, it was confirmed that a decrease of about 32% was observed due to this distance difference. Therefore, the actual clinical application of the selected catheter can be used as a substitute for the existing rectal retractor and wet gauze.