• 한국작물학회지
• /
• 제44권4호
• /
• pp.345-349
• /
• 1999

This study was carried out to map Bphl and bph2 gene in Mudgo and Sangju13 (Oryza sativa L.) respectively conferring resistance to brown plan-thopper (BPH) and to establish the marker-assisted selection (MAS) system. Bulked seedling (grown for 20 days) test was conducted with the 73 F4 lines derived from a cross between Nagdongbyeo and Mudgo for Bphl and with 53 BC3F5 lines derived from the Milyang95/Sangju13 cross for bph2. Bph1 was mapped between RG413 and RG901 on chromo-some 12 at a distance of 7.5 cM from RG413 and 8.4 cM from RG90l. A recessive gene bph2 was located near RZ76 on chromosome 12 at a distance of 14.4 cM. Bphl and bph2 were linked to each other with a distance of about 30 cM. An RFLP marker, RG413 linked to Bphl, was converted to an STS marker to facilitate the marker-assisted selection. BPH resistant genotypes could be selected with 92% accuracy in a population derived from a line of NM47-B-B.

• The Plant Pathology Journal
• /
• 제26권2호
• /
• pp.159-169
• /
• 2010

Lesion mimic mutant (LMM) plants display spontaneous necrotic lesions on their leaves without any pathogenic infection. Specific rice LMMs designated as spotted leaf (spl) including spl1, spl3, spl4, spl5 and spl6 are genetically known as lesion resembling disease (lrd) mutant. The inheritance patterns in the $F_1$ and $F_2$ progenies of these mutants are controlled by recessive genetic factors. Lesion development in the rice LMMs were controlled by both development stages and environmental factors. The rice LMMs exhibited higher numbers of spots under $45^{\circ}C$ temperature than those under $30^{\circ}C$. Contents of chlorophyll were drastically reduced at 60 days old LMM leaves when the spot formation was severe. The levels of endogenous hydrogen peroxide were highest at 45 days old mutants but reduced at 60 days old. Transcription levels of stress related genes including thioredoxin peroxidase and protein disulfide isomerase were reduced in spotted leaves than those of non spotted leaves. It could be suggested that scavenging system against reactive oxygen species induced by either stresses or innate metabolisms may not work properly in the rice LMMs. As these rice LMMs autonomously expressed clear lesions of lrd phenotype without pathogen infection, it could be useful to understand stresses responses in plants.

• Journal of Korean Neurosurgical Society
• /
• 제49권1호
• /
• pp.8-12
• /
• 2011

Objective: Ossification of the posterior longitudinal ligament (OPLL) has a strong genetic component. Specific gene polymorphisms may be associated with OPLL in several genes which regulate calcification in chondrocytes, change of extracellular collagen matrix and secretions of many growth factors and cytokines controlling bone morphogenesis. Toll-like receptor 5 (TLR5) may playa role in the pathogenesis of OPLL by intermediate nuclear factor-kappa B (NF-${\kappa}B$). The current study focused on coding single nucleotide polymorphisms (SNPs) of TLR5 for a case-control study investigating the relationship between TLR5 and OPLL in a Korean population. Methods: A total of 166 patients with OPLL and 231 controls were recruited for a case-control association study investigating the relationship between SNPs of TLR5 gene and OPLL. Four SNPs were genotyped by direct sequencing (rs5744168, rs5744169, rs2072493, and rs5744174). SNP data were analyzed using the SNPStats, SNPAnalyzer, Haploview, and Helixtree programs. Multiple logistic regression analysis with adjustment for age and gender was performed to calculate an odds ratio (OR). Results: None of SNPs were associated with OPLL in three alternative models (codominant, dominant, and recessive models; p> 0.05). A strong linkage disequilibrium block, including all 4 SNPs, was constructed using the Gabriel method. No haplotype was significantly associated with OPLL in three alternative models. Conclusion: These results suggest that Toll-like receptor 5 gene may not be associated with ossification of the posterior longitudinal ligament risk in Korean population.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권7호
• /
• pp.2713-2717
• /
• 2015

Background: Possible associations between the single nucleotide polymorphism (SNP) rs8034191 in the aminoglycosidephosphotransferase domain containing 1 (AGPHD1) gene and lung cancer risk have been studied by many researchers but the results have been contradictory. Materials and Methods: A computerized search for publications on rs8034191 and lung cancer risk was performed. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the association between rs8034191 and lung cancer risk with 13 selected case-control studies. Sensitivity analysis, test of heterogeneity, cumulative meta-analysis, and assessment of bias were also performed. Results: A significant association between rs8034191 and lung cancer susceptibility was found using the dominant genetic model (OR=1.344, 95% CI: 1.285-1.406), the additive genetic model (OR=1.613, 95% CI: 1.503-1.730), and the recessive genetic model (OR=1.408, 95% CI: 1.319-1.503). Moreover, an increased lung cancer risk was found with all genetic models after stratification of ethnicity. Conclusions: The association between rs8034191 and lung cancer risk was significant using multiple genetic models, suggesting that rs8034191 is a risk factor for lung cancer. Further functional studies of this polymorphism and lung cancer risk are warranted.

• Journal of Genetic Medicine
• /
• 제6권2호
• /
• pp.170-174
• /
• 2009

저자들은 생후 3개월에 전형적인 페닐케톤뇨증으로 진단받고 식사요법을 유지하였으나 지연된 발달 소견 및 지능 저하를 보이고 경련 증상이 있었던 9세 남자 환아에서 효소 검사와 유전자분석으로 dihydropterine reductase (DHPR) 결핍증을 진단하였다. 그리고 $BH_4$, 신경전달물질 전구체 투여 및 엽산 보충으로 DHPR 결핍증을 치료한 1례를 경험하였기에 문헌고찰과 함께 보고한다.

• 한국응용곤충학회지
• /
• 제45권3호
• /
• pp.283-291
• /
• 2006

누에의 가시 돌연변이 형질을 이용한 환경변이원 검색계 수립을 위하여 MMC, EMS, PCB에 대한 누에알 변이원 검색계의 감수성을 조사한 결과, 난모세포계는 MMC, 정세포계는 EMS에 대하여 특정적으로 높은 감수성을 보였고, PCB와 dioxin에 대해서도 양성반응을 얻을 수 있었다. 수나방의 사정횟수에 따른 정세포의 변이원 감수성 변화를 시험한 결과, 1교와 3교 때 사정된 정자 간에는 큰 차이가 없었다. 변이원 감수성은 높으나, 산란성, 우화율 등 검색계로서 주요형질이 극히 불량한 선3호는 C5, Nl2, 한삼면과의 교잡으로 주요형질이 개선되고 높은 변이원 감수성을 유지하였다.

• 한국응용곤충학회지
• /
• 제20권1호
• /
• pp.15-20
• /
• 1981

벼 품종에 대한 가해능력을 달리하는 벼멸구 생태형의 저항성품종에서의 우화율 유전양상을 분석하고자 실내에서 저항성 품종(Mudgo, ASD 7)을 식이로 이대사육해 인위적으로 유발시킨 유발생태형 2 및 3과 기존생태형 1 사이의 상호 정역교배를 실시하여 $P_1,\;P_2,\;F_l\; F_2,\;BC_1$ 및 $BC_2$의 각세대 $1\~2$령 약충을 공시품종인 Mudgo, ASD 7 $1\~2$엽기 유묘에 접종하여 그 우화율을 조사하였다. 우화율에 대한 유전분석 결과, Mudgo에서의 생태형 2와 생태형 1사이의 조합에서는 생태형 2가 불완전 우성의 단일유전자 ASD 7에서의 생태형 3과 생태형 1 사이의 조합에서는 생태형 3이 불완전열성의 단일 유전자에 의해 우화율이 지배되며, 생태형 2와 생태형 3의 대립관계는 불명확하였다.

• 한국연초학회지
• /
• 제22권1호
• /
• pp.71-75
• /
• 2000

The genetic makeup could be the most important among many factors affecting yield and quality of tobacco(Nicotiana tabacum L.). The mammoth gene found in N. tabacum is associated with greater leaf number and poor leaf quality. This study was carried out to obtain the basic information about the inheritance of mammoth gene and white flower color. Two flue-cured breeding lines, KF 9373-2 and KF 8832-85, F$_1$, F$_2$, two parents backcrossed with F$_1$, and F$_3$ lines derived from cross of above two lines were investigated for flowering type(mammoth gene) and flower color. All plants of F$_1$ population revealed normal flowering type and pink flower color. The progeny of F$_2$ generation was segregated into the phenotypic ratio of 9 : 3 : 3 : 1 with normal flowering type and pink flower color, normal and white, non flowering type(NF) and pink, and NF and white, respectively. Among the progenies of back-crossing populations, the flowering type showed a segregation ratio of 1 : 1 as normal and NF in BP$_1$ and flower color did also 1 : 1 as pink and white in BP$_2$. All lines have the mammoth gene in F$_3$. that were selected in F$_2$ progeny as non flowering. But 9 lines among 14 were segregated with 3 : 1 as pink and white flower in F$_3$. which were selected in F$_2$ as pink flower color. These results indicated that the characters of mammoth gene and white flower were controlled by a pair of recessive genes, respectively.

• 생명과학회지
• /
• 제7권1호
• /
• pp.49-58
• /
• 1997

Engralied 5.7kb upstream sequence와 E. colilacZ의 융합 유전자를 가진 P[en-lacZ] 인자를 jumpstart 기법을 이용하여, ryXho25 strain의 초파리 48A 염색체 위치로부터 새로운 위치로 삽입하였다. 총 3315의 유전적 교배를 통해서, P[en-lacZ] 가 다른 염색체 상으로 삽인된 113 계통을 얻었다. X-gal 염색으로 이들 113 계통의 3령기 유충 조직에서의 $\beta$-galactosidase 발현을 조사하였다. 도한 113 계통 중 7계통이 열성치사돌연변이인 것으로 동정되었다. 이들 7 계통 중 초기 배발생 과정에서 치사하는 것으로 조사된 #1119의 초기 배발생 과정에서의 ${\beta}$-galactosidase 발현과 핵의 이동 및 세포화 양상을 조사하였다. 본 연구에서 얻어진 P[en-lacZ] 삽입 돌연변이체들은 앞으로 Drosophila 발생에 관련된 유전자들의 구조와 기능을 연구하는데 활용될 수 있을 것이다.

• Genomics & Informatics
• /
• 제7권1호
• /
• pp.13-19
• /
• 2009

Osteoporosis is characterized by impaired osteogenesis. BMD is a major determinant of bone strength. The role of the VDR gene in predisposition to primary osteoporosis has been recognized. However, population-based case-control studies have been reported controversial results for known candidate genes in an ethnically distinct group. To determine the genetic effects of VDR variants on osteoporosis and BMD, we directly sequenced the VDR gene in 24 unrelated Korean individuals and identified eighteen sequence variants. We investigated the potential involvement of eight SNPs in osteoporosis in postmenopausal women (n = 729). Two SNPs (LD) in intron 2, -5294G>C (rs2238135) and -4817G>A (rs17882443) showed the evidence of association with enhanced BMD of the femoral neck ($p_{additive}$=0.031 for rs2238135; $p_{additive}$=0.017 and $p_{dominant}$= 0.019 for 17882443). Moreover, VDR -4817G>A was significantly associated with protective effect on all fracture risk ($p_{recessive}$=0.035, OR=0.2, 95% CI=$0.05{\sim}0.89$), and tended to be higher BMD values at various proximal femur sites. Therefore, we suggest that the -4817G>A may be useful genetic marker for vitamin D-related metabolism and may have an important role in the increased BMD of the proximal femur in postmenopausal Korean women.